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Brazilian Dental Journal 2021The aim of this review is to discuss the digital planning and the use of guided technology in Endodontics. The complexity of the root canals anatomy and the challenges... (Review)
Review
The aim of this review is to discuss the digital planning and the use of guided technology in Endodontics. The complexity of the root canals anatomy and the challenges in the microorganism's control represent risk factors for failure after the infected root canal's treatment. Scientific improvements associated with technological advances have enabled better predictability of therapeutic procedures results. The development of efficient and modern devices provided safer root canal treatments, with shorter clinical visits and greater patient comfort. Digital endodontics incorporated different tools and developed its own, advancing even further in resolving complex cases. The faithful copy of the internal anatomy provided by the advancement of CBCT devices and software's, associated with the digital resources of 3D planning and printing, enabled the advent of guided endodontics. This technique is used at different stages of endodontic treatment, with specific indications and greater result predictability. Therefore, this study critically reviewed the potential clinical application of this guided access technique, and the operative steps for its safe performance in managing complex endodontic cases. The main indications are accessing calcified root canals, performing endodontic surgeries in difficult access areas, removing fiberglass posts, and accessing teeth with developmental anomalies. In summary, guided endodontics has been a precise strategy, effective, safe, and clinically applicable. This procedure represents incorporating technological resources and digital planning in the Endodontist clinical practice, increasing predictability to complex cases.
Topics: Cone-Beam Computed Tomography; Dental Pulp Cavity; Endodontics; Humans; Root Canal Therapy; Technology
PubMed: 34877975
DOI: 10.1590/0103-6440202104740 -
Methods in Molecular Biology (Clifton,... 2017Computational approaches are useful tools to interpret and guide experiments to expedite the antibiotic drug design process. Structure-based drug design (SBDD) and...
Computational approaches are useful tools to interpret and guide experiments to expedite the antibiotic drug design process. Structure-based drug design (SBDD) and ligand-based drug design (LBDD) are the two general types of computer-aided drug design (CADD) approaches in existence. SBDD methods analyze macromolecular target 3-dimensional structural information, typically of proteins or RNA, to identify key sites and interactions that are important for their respective biological functions. Such information can then be utilized to design antibiotic drugs that can compete with essential interactions involving the target and thus interrupt the biological pathways essential for survival of the microorganism(s). LBDD methods focus on known antibiotic ligands for a target to establish a relationship between their physiochemical properties and antibiotic activities, referred to as a structure-activity relationship (SAR), information that can be used for optimization of known drugs or guide the design of new drugs with improved activity. In this chapter, standard CADD protocols for both SBDD and LBDD will be presented with a special focus on methodologies and targets routinely studied in our laboratory for antibiotic drug discoveries.
Topics: Computer-Aided Design; Databases as Topic; Drug Design; Drug Evaluation, Preclinical; Ligands; Molecular Docking Simulation; Quantitative Structure-Activity Relationship; Thermodynamics; User-Computer Interface
PubMed: 27873247
DOI: 10.1007/978-1-4939-6634-9_5 -
Microorganisms Oct 2023Nanomaterials are used to develop simpler, cheaper, and faster methods for disease diagnosis [...].
Nanomaterials are used to develop simpler, cheaper, and faster methods for disease diagnosis [...].
PubMed: 37894187
DOI: 10.3390/microorganisms11102530 -
Metabolites Nov 2022In the face of climate change, progressive degradation of the environment, including agricultural land negatively affecting plant growth and development, endangers plant... (Review)
Review
In the face of climate change, progressive degradation of the environment, including agricultural land negatively affecting plant growth and development, endangers plant productivity. Seeking efficient and sustainable agricultural techniques to replace agricultural chemicals is one of the most important challenges nowadays. The use of plant growth-promoting microorganisms is among the most promising approaches; however, molecular mechanisms underneath plant-microbe interactions are still poorly understood. In this review, we summarized the knowledge on plant-microbe interactions, highlighting the role of microbial and plant proteins and metabolites in the formation of symbiotic relationships. This review covers rhizosphere and phyllosphere microbiomes, the role of root exudates in plant-microorganism interactions, the functioning of the plant's immune system during the plant-microorganism interactions. We also emphasized the possible role of the stringent response and the evolutionarily conserved mechanism during the established interaction between plants and microorganisms. As a case study, we discussed fungi belonging to the genus . Our review aims to summarize the existing knowledge about plant-microorganism interactions and to highlight molecular pathways that need further investigation.
PubMed: 36422239
DOI: 10.3390/metabo12111100 -
Briefings in Bioinformatics Mar 2021Humans have coexisted with pathogenic microorganisms throughout its history of evolution. We have never halted the exploration of pathogenic microorganisms. With the... (Review)
Review
Humans have coexisted with pathogenic microorganisms throughout its history of evolution. We have never halted the exploration of pathogenic microorganisms. With the improvement of genome-sequencing technology and the continuous reduction of sequencing costs, an increasing number of complete genome sequences of pathogenic microorganisms have become available. Genome annotation of this massive sequence information has become a daunting task in biological research. This paper summarizes the approaches to the genome annotation of pathogenic microorganisms and the available popular genome annotation tools for prokaryotes, eukaryotes and viruses. Furthermore, real-world comparisons of different annotation tools using 12 genomes from prokaryotes, eukaryotes and viruses were conducted. Current challenges and problems were also discussed.
Topics: Eukaryota; Genome, Bacterial; Genome, Viral; Humans; Molecular Sequence Annotation; Virulence
PubMed: 33537706
DOI: 10.1093/bib/bbab004 -
Biotechnology Letters May 2019Mupirocin is an antibiotic from monocarboxylic acid class used as antibacterial agent against methicillin-resistant Staphylococcus aureus (MRSA) and can be obtained as a... (Review)
Review
Mupirocin is an antibiotic from monocarboxylic acid class used as antibacterial agent against methicillin-resistant Staphylococcus aureus (MRSA) and can be obtained as a mixture of four pseudomonic acids by Pseudomonas fluorescens biosynthesis. Nowadays improving antibiotics occupies an important place in the pharmaceutical industry as more and more resistant microorganisms are developing. Mupirocin is used to control the MRSA outbreaks, for infections of soft tissue or skin and for nasal decolonization. Due to its wide use without prescription, the microorganism's resistance to Mupirocin increased from up to 81%, thus becoming imperative its control or improvement. As the biotechnological production of Mupirocin has not been previously reviewed, in the present paper we summarize some consideration on the biochemical process for the production of pseudomonic acids (submerged fermentation and product recovery). Different strains of Pseudomonas, different culture medium and different conditions for the fermentation were analysed related to the antibiotics yield and the product recovery step is analysed in relation to the final purity. However, many challenges have to be overcome in order to obtain pseudomonic acid new versions with better properties related to antibacterial activity.
Topics: Anti-Bacterial Agents; Carrier State; Drug Resistance, Bacterial; Fermentation; Humans; Methicillin-Resistant Staphylococcus aureus; Mupirocin; Pseudomonas fluorescens; Soft Tissue Infections; Staphylococcal Infections; Staphylococcal Skin Infections; Technology, Pharmaceutical
PubMed: 30927135
DOI: 10.1007/s10529-019-02670-w -
Sheng Wu Gong Cheng Xue Bao = Chinese... Feb 2022Monoterpenoids that belong to the terpenoids family are usually volatile and have strong aroma. Some monoterpenoids also have antioxidant, antibacterial and... (Review)
Review
Monoterpenoids that belong to the terpenoids family are usually volatile and have strong aroma. Some monoterpenoids also have antioxidant, antibacterial and anti-inflammatory activities, which make them important raw materials for medicine, food and cosmetics industry. In recent years, the heterologous synthesis of monoterpenoids by microorganisms has attracted extensive attention. However, its large-scale application is greatly hampered by the low yield and high production cost. Nowadays, the rapid development of synthetic biology provides new approaches for enhancing the production of monoterpenoids by microorganisms. Different kinds of recombinant strains can be obtained via engineering of microbial cells to produce a variety of monoterpenoids with different properties. This paper summarized the latest strategies and progress in the application of synthetic biology to produce monoterpenoids by microorganisms, including the design and modification of biosynthetic pathway, as well as the design and optimization of high-yield monoterpenoids producing chassis cells.
Topics: Biosynthetic Pathways; Metabolic Engineering; Monoterpenes; Synthetic Biology; Terpenes
PubMed: 35234374
DOI: 10.13345/j.cjb.210145 -
Nanoscale Mar 2023This review presents a comprehensive summary of the material-microorganism interface in microbial hybrid electrocatalysis systems. Microbial hybrid electrocatalysis has... (Review)
Review
This review presents a comprehensive summary of the material-microorganism interface in microbial hybrid electrocatalysis systems. Microbial hybrid electrocatalysis has been developed to combine the advantages of inorganic electrocatalysis and microbial catalysis. However, electron transfer at the interfaces between microorganisms and materials is a very critical issue that affects the efficiency of the system. Therefore, this review focuses on the electron transfer at the material-microorganism interface and the strategies for building efficient microorganism and material interfaces. We begin with a brief introduction of the electron transfer mechanism in both the bioanode and biocathode of bioelectrochemical systems to understand the material-microorganism interface. Next, we summarise the strategies for constructing efficient material-microorganism interfaces including material design and modification and bacterial engineering. We also discuss emerging studies on the bio-inorganic hybrid electrocatalysis system. Understanding the interface between electrode/active materials and the microorganisms, especially the electron transfer processes, could help to drive the evolution of material-microorganism hybrid electrocatalysis systems towards maturity.
Topics: Electron Transport; Catalysis; Electrodes; Bacteria
PubMed: 36912348
DOI: 10.1039/d3nr00742a -
International Journal of Environmental... 2022The pollutants have become ubiquitous in the total environment (water, soil and air) due to human activities and they are hazardous to all forms of life on the earth.... (Review)
Review
The pollutants have become ubiquitous in the total environment (water, soil and air) due to human activities and they are hazardous to all forms of life on the earth. This problem has made scientists focus on mitigating or complete reduction in pollutants by several means. Microorganism and plants are known to scavenge pollutants. Both are studied enormously in reducing, refining, and removing pollutants from the environment successfully. But, their slow process for removal is disadvantage. However, according to recent advancements in the abatement of pollutants, a combined system of both microorganisms and plant has shown to enhance the remediation of pollutants to an efficient level. In a nutrient-depleted pollutant-rich environment, when suitable plant and microorganisms are introduced, the plant interacts with the rhizosphere and root associate with microorganisms to survive in toxic conditions. The chemicals released by plants signal the microorganisms for interactions. This interaction leads in higher germination efficiency and enhanced root elongation which results in enhanced degradation of pollutants in both rhizosphere and phyllosphere. In this background, the current review article provides an overview of the recent advancement in microorganisms plant combined systems in enhanced removal of several recalcitrant pollutants. The conclusion highlights the challenges and future perspectives in this area of research.
PubMed: 34122578
DOI: 10.1007/s13762-021-03354-7 -
International Journal of Molecular... Nov 2023Pesticides are chemicals used in agriculture, forestry, and, to some extent, public health. As effective as they can be, due to the limited biodegradability and toxicity... (Review)
Review
Pesticides are chemicals used in agriculture, forestry, and, to some extent, public health. As effective as they can be, due to the limited biodegradability and toxicity of some of them, they can also have negative environmental and health impacts. Pesticide biodegradation is important because it can help mitigate the negative effects of pesticides. Many types of microorganisms, including bacteria, fungi, and algae, can degrade pesticides; microorganisms are able to bioremediate pesticides using diverse metabolic pathways where enzymatic degradation plays a crucial role in achieving chemical transformation of the pesticides. The growing concern about the environmental and health impacts of pesticides is pushing the industry of these products to develop more sustainable alternatives, such as high biodegradable chemicals. The degradative properties of microorganisms could be fully exploited using the advances in genetic engineering and biotechnology, paving the way for more effective bioremediation strategies, new technologies, and novel applications. The purpose of the current review is to discuss the microorganisms that have demonstrated their capacity to degrade pesticides and those categorized by the World Health Organization as important for the impact they may have on human health. A comprehensive list of microorganisms is presented, and some metabolic pathways and enzymes for pesticide degradation and the genetics behind this process are discussed. Due to the high number of microorganisms known to be capable of degrading pesticides and the low number of metabolic pathways that are fully described for this purpose, more research must be conducted in this field, and more enzymes and genes are yet to be discovered with the possibility of finding more efficient metabolic pathways for pesticide biodegradation.
Topics: Humans; Pesticides; Biodegradation, Environmental; Bacteria; Fungi; Agriculture
PubMed: 37958952
DOI: 10.3390/ijms242115969