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The Journal of Dermatology Oct 2018
Topics: Administration, Cutaneous; Combined Modality Therapy; Cryotherapy; DNA, Viral; Humans; Imiquimod; Male; Middle Aged; Molluscum Contagiosum; Molluscum contagiosum virus; Nitrogen; Penis; Skin; Treatment Outcome
PubMed: 29667708
DOI: 10.1111/1346-8138.14319 -
Virus Genes Aug 2017The molluscum contagiosum virus (MCV) uses a variety of immune evasion strategies to antagonize host immune responses. Two MCV proteins, MC159 and MC160, contain tandem...
The molluscum contagiosum virus (MCV) uses a variety of immune evasion strategies to antagonize host immune responses. Two MCV proteins, MC159 and MC160, contain tandem death effector domains (DEDs). They are reported to inhibit innate immune signaling events such as NF-κB and IRF3 activation, and apoptosis. The RxDL motif of MC159 is required for inhibition of both apoptosis and NF-κB activation. However, the role of the conserved RxDL motif in the MC160 DEDs remained unknown. To answer this question, we performed alanine mutations to neutralize the arginine and aspartate residues present in the MC160 RxDL in both DED1 and DED2. These mutations were further modeled against the structure of the MC159 protein. Surprisingly, the RxDL motif was not required for MC160's ability to inhibit MAVS-induced IFNβ activation. Further, unlike previous results with the MC159 protein, mutations within the RxDL motif of MC160 had no effect on the ability of MC160 to dampen TNF-α-induced NF-κB activation. Molecular modeling predictions revealed no overall changes to the structure in the MC160 protein when the amino acids of both RxDL motifs were mutated to alanine (DED1 = R67A D69A; DED2 = R160A D162A). Taken together, our results demonstrate that the RxDL motifs present in the MC160 DEDs are not required for known functions of the viral protein.
Topics: Amino Acid Motifs; Apoptosis; Humans; Immune Evasion; Interferon-beta; Molluscum Contagiosum; Molluscum contagiosum virus; Protein Domains; Tumor Necrosis Factor-alpha; Viral Proteins
PubMed: 28425034
DOI: 10.1007/s11262-017-1456-9 -
The British Journal of Dermatology Aug 2019
Topics: Adult; Antibodies, Monoclonal, Humanized; Cantharidin; Cryotherapy; Dermatitis, Atopic; Drug Resistance, Viral; Drug Substitution; Humans; Imiquimod; Immunosuppressive Agents; Interleukin-4 Receptor alpha Subunit; Male; Molluscum Contagiosum; Molluscum contagiosum virus; Treatment Outcome
PubMed: 30719708
DOI: 10.1111/bjd.17712 -
Virology May 2017Apoptosis is a powerful host cell defense to prevent viruses from completing replication. Poxviruses have evolved complex means to dampen cellular apoptotic responses....
Apoptosis is a powerful host cell defense to prevent viruses from completing replication. Poxviruses have evolved complex means to dampen cellular apoptotic responses. The poxvirus, Molluscum Contagiosum Virus (MCV), encodes numerous host interacting molecules predicted to antagonize immune responses. However, the function of the majority of these MCV products has not been characterized. Here, we show that the MCV MC163 protein localized to the mitochondria via an N-terminal mitochondrial localization sequence and transmembrane domain. Transient expression of the MC163 protein prevented mitochondrial membrane permeabilization (MMP), an event central to cellular apoptotic responses, induced by either Tumor Necrosis Factor alpha (TNF-α) or carbonyl cyanide 3-chlorophenylhydrazone (CCCP). MC163 expression prevented the release of a mitochondrial intermembrane space reporter protein when cells were challenged with TNF-α. Inhibition of MMP was also observed in cell lines stably expressing MC163. MC163 expression may contribute to the persistence of MCV lesions by dampening cellular apoptotic responses.
Topics: Apoptosis; Caspase 3; Cell Line, Tumor; Cell Membrane Permeability; HeLa Cells; Humans; Hydrazones; Mitochondria; Mitochondrial Membranes; Molluscum Contagiosum; Molluscum contagiosum virus; Poly (ADP-Ribose) Polymerase-1; Staurosporine; Tumor Necrosis Factor-alpha; Viral Proteins
PubMed: 28235685
DOI: 10.1016/j.virol.2017.02.017 -
Autoinoculation as a treatment modality for molluscum contagiosum: A preliminary uncontrolled trial.Indian Journal of Dermatology,... 2018
Topics: Follow-Up Studies; Humans; Immunotherapy; Molluscum Contagiosum; Molluscum contagiosum virus; Prospective Studies; Treatment Outcome
PubMed: 29251278
DOI: 10.4103/ijdvl.IJDVL_1033_16 -
Dermatology Online Journal Jan 2016A three-year-old boy presented to our oculoplastic clinic with complaints of painless gradually increasing right upper lid mass for the last 6 months. On examination a...
A three-year-old boy presented to our oculoplastic clinic with complaints of painless gradually increasing right upper lid mass for the last 6 months. On examination a firm mass measuring roughly 1x1 cm was present on the upper lid. The mass was non tender with fine superficial vessels running over it. A differential diagnosis of epidermoid cyst, vascular malformation, pilomatrixoma, and juvenile xanthogranuloma was considered. The patient underwent excisional biopsy of the mass. On gross examination the mass had a brain like appearance. Histopathological examination confirmed the diagnosis of molluscum contagiosum. It is rare for molluscum contagiosum to present as a solitary lid tumor. A brain like appearance of the excised mass can provide a clue towards the diagnosis.
Topics: Antibodies, Viral; Child, Preschool; Dermatologic Surgical Procedures; Diagnosis, Differential; Eyelid Neoplasms; Eyelids; Humans; Immunocompromised Host; Male; Molluscum Contagiosum; Molluscum contagiosum virus; Skin Diseases, Viral
PubMed: 26990479
DOI: No ID Found -
The British Journal of Dermatology Mar 2019
Topics: Child; Dermoscopy; Female; Humans; Microscopy, Electron, Transmission; Molluscum Contagiosum; Molluscum contagiosum virus; Perineum; Skin
PubMed: 30821395
DOI: 10.1111/bjd.17401 -
Journal Francais D'ophtalmologie May 2020
Topics: Adult; Conjunctivitis, Viral; Female; Humans; Immunocompetence; Molluscum Contagiosum; Molluscum contagiosum virus
PubMed: 32327278
DOI: 10.1016/j.jfo.2019.08.021 -
International Journal of STD & AIDS Sep 2015
Topics: Adolescent; Anti-Bacterial Agents; Contact Tracing; Disease Management; Female; HIV Infections; Humans; Male; Molluscum Contagiosum; Molluscum contagiosum virus; Practice Guidelines as Topic; Reproductive Health; United Kingdom
PubMed: 25332225
DOI: 10.1177/0956462414554435