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Environmental Science and Pollution... Jan 2020Monocrotophos (MCP) is a broad spectrum organophosphorus insecticide, which is widely used as foliar spray to the different important crops. MCP may reach the soil and...
Establishment of resveratrol and its derivatives as neuroprotectant against monocrotophos-induced alteration in NIPBL and POU4F1 protein through molecular docking studies.
Monocrotophos (MCP) is a broad spectrum organophosphorus insecticide, which is widely used as foliar spray to the different important crops. MCP may reach the soil and the aquatic environment directly or indirectly during and after the application, which leads to the different environmental issues. MCP is found to be associated with neurotoxicity and its toxic effects have been monitored during different stages of neuronal development. Identification of gene expression in MCP-induced neurotoxicity during neuronal developmental stage is a major area of genomic research interest. In accordance with this identification, screening of potential neuroprotective, natural resources are also required as a preventive aspects by targeting the impaired genes. In this current course of work, microarray experiment has been used to identify genes that were expressed in monocrotophos (MCP)-induced mesenchymal stem cells (MSC) and also the neuroprotectant activity of RV on MCP-exposed MSCs. Microarray experiment data have been deposited in NCBI's Gene Expression Omnibus database and are accessible through GEO Series accession number GSE121261. In this paper, we have discussed two important genes NIPBL (nipped-B-like protein) and POU4F1 (POU domain, class 4, transcription factor 1). These genes were found to be significantly expressed in MCP-exposed MSC and show minimum expression in presence of RV. Homology modelling and docking study was done to identify the interaction and binding affinity of resveratrol and its derivatives with NIPBL and POU4F1 protein. Docking analysis shows that RV and its derivatives have strong interaction with NIPBL and POU4F1 protein hence proves the significance of resveratrol as potential neuroprotectant. This paper highlights the hazardous impact of MCP on neuronal development disorders and repairing potentiality of RV and its derivatives on altered genes involved in neuronal diseases. Graphical Abstract.
Topics: Cell Cycle Proteins; Genes, cdc; Humans; Insecticides; Mesenchymal Stem Cells; Molecular Docking Simulation; Monocrotophos; Neurons; Neuroprotective Agents; Resveratrol; Transcription Factor Brn-3A
PubMed: 31786755
DOI: 10.1007/s11356-019-06806-3 -
International Journal of Environmental... Apr 2020Earthworms are an ideal biological model in toxicity assays and environmental monitoring studies. In the present study, the reproductive toxicity and histopathological...
Earthworms are an ideal biological model in toxicity assays and environmental monitoring studies. In the present study, the reproductive toxicity and histopathological effects of Monocrotophos pesticide on an exotic epigeic and an indigenous epigeic earthworm were studied. Earthworm species were exposed to different concentrations of pesticide like 450 ppm, 500 ppm, and 650 ppm for 45 days and the mortality rate and reproductive activity was recorded every 15 days of exposure. There was an increase in mortality and abnormal sperm (asthenospermia, necrospermia, and oligospermia) and defective cocoons in earthworms with increasing concentrations of the pesticide. Histopathological changes like rupture of chloragogenous tissue, longitudinal muscle, fused and extra-villous growth and necrotic cell rupture in earthworm's body wall (epidermis, circular and longitudinal muscles) were observed. Fluorescent probes have detected cell death in pesticide-treated earthworms when compared to the control group after 45 days. The present findings show that Monocrotophos pesticide on exposure to epigeic earthworm species causes significant reproductive toxicity and histopathological abnormalities and these changes could be used as a tool in environmental risk assessment of pesticides.: DDT: Dichlorodiphenyltrichloroethane; MCP: Monocrotophos; EPA: Environment Protection Act; SL: Soluble Liquid; C: N (Carbon: Nitrogen); C: P (Carbon: Phosphorus); LC: Lethal Concentration; PBS: Phosphate Buffer Solution; WHO: World Health Organization; H&E: Hematoxylin and Eosin; SV: seminal vesicles; O: ovary; GP: genital papillae; Ch: chloragogenous tissue; EL: epithelial layer; CM: circular muscle; LM: longitudinal muscle; CD: cell debris.
Topics: Animals; Insecticides; Introduced Species; Monocrotophos; Oligochaeta; Reproduction; Sexual Behavior, Animal; Soil Pollutants
PubMed: 30897939
DOI: 10.1080/09603123.2019.1590538 -
Molecular Neurobiology Jul 2017Expression of various cytochrome P450s (CYPs) in mammalian brain cells is well documented. However, such studies are hampered in neural/glial cells of human origin due...
Expression of various cytochrome P450s (CYPs) in mammalian brain cells is well documented. However, such studies are hampered in neural/glial cells of human origin due to nonavailability of human brain cells. To address this issue, we investigated the expression and inducibility of CYP2C8 and CYP3A4 and their responsiveness against cyclophosphamide (CPA) and organophosphorus pesticide monocrotophos (MCP), a known developmental neurotoxicant in human neural (SH-SY5Y) and glial (U373-MG) cell lines. CPA induced significant expression of CYP2C8 and CYP3A4 in both types of cells in a time-dependent manner. Neural cell line exhibited relatively higher constitutive and inducible expression of CYPs than the glial cell line. MCP exposure alone could not induce the significant expression of CYPs, whereas the cells preexposed to CPA showed a significant response to MCP. Similar to the case of CPA induced expressions, neural cells were found to be more vulnerable than glial cells. Our data indicate differential expressions of CYPs in cultured human neural and glial cell lines. The findings were synchronized with protein ligand docking studies, which showed a significant modulatory capacity of MCP by strong interaction with CYP regulators-CAR and PXR. Similarly, the known CYP inducer CPA has also shown significant high docking scores with the two studied CYP regulators. We also observed a significant induction in reactive oxygen species (ROS), lipid peroxides (LPO), micronucleus (MN), chromosomal aberration (CA), and reduction in reduced glutathione (GSH) and catalase following the exposure of MCP. Moreover, the expressions of apoptotic markers such as caspase-3, caspase-9, Bax, and p53 were significantly upregulated, whereas the levels of antiapoptotic marker, Bcl2, was downregulated after the exposure of MCP in both cell lines. These findings confirm the involvement of ROS-mediated oxidative stress, which subsequently triggers apoptosis pathways in both human neural (SH-SY5Y) and glial (U373-MG) cell lines following the exposure of MCP.
Topics: Apoptosis; Brain; Caspases; Catalase; Cell Death; Cell Line; Constitutive Androstane Receptor; Cyclophosphamide; Cytochrome P-450 CYP2C8; Cytochrome P-450 CYP3A; Cytochromes c; Enzyme Induction; Humans; Ligands; Lipid Peroxidation; Micronucleus Tests; Molecular Docking Simulation; Monocrotophos; Neurotoxins; Oxidative Stress; Pregnane X Receptor; Protein Biosynthesis; Reactive Oxygen Species; Receptors, Cytoplasmic and Nuclear; Receptors, Steroid; Transcription, Genetic; Xenobiotics; bcl-2-Associated X Protein
PubMed: 27206429
DOI: 10.1007/s12035-016-9938-7 -
Chemosphere Jul 2023With the aim of monocrotophos pesticides detection in environmental and food samples at point-of-care (PoC) application, this research, for the first time, explores...
With the aim of monocrotophos pesticides detection in environmental and food samples at point-of-care (PoC) application, this research, for the first time, explores silica alcogel as an immobilization matrix to support the development of in-house customized nano-enabled "chromagrid-lighbox" as a sensing system. This system is fabricated using laboratory waste materials and demonstrates the detection of highly hazardous monocrotophos pesticide using a smartphone. Nano-enabled chromagrid is a chip-like assembly filled with silica alcogel -a nanomaterial (hence the name "nano-enabled" chromagrid), and "chromogenic reagents" which is required for the enzymatic detection of monocrotophos. Lightbox is the imaging station fabricated to provide constant lighting conditions to the chromagrid to capture accurate colorimetric data. The silica alcogel used in this system was synthesized from Tetraethyl orthosilicate (TEOS) via a sol-gel method and characterized using advanced analytical techniques. Further, three chromagrid assays were developed for the optical detection of monocrotophos with a low detection limit (LOD) at 0.421 ng ml (by α-NAc chromagrid assay), 0.493 ng ml (by DTNB chromagrid assay) and 0.811 ng ml (by IDA chromagrid assay). The developed novel PoC chromagrid-lightbox system is capable of on-site detection of monocrotophos in environmental as well as food samples. This system is able to be manufacture prudently using recyclable waste plastic. Overall, such developed eco-friendly PoC testing system will surely manage rapid detection of monocrotophos pesticide needed for environmental and sustainable agricultural management.
Topics: Pesticides; Monocrotophos; Point-of-Care Systems; Smartphone; Silicon Dioxide
PubMed: 37100248
DOI: 10.1016/j.chemosphere.2023.138704 -
Environmental Technology Feb 2020In the present study, a biosensor was developed for the detection of organophosphorous compounds. Core electrode of a working electrode was obtained by depositing the...
In the present study, a biosensor was developed for the detection of organophosphorous compounds. Core electrode of a working electrode was obtained by depositing the paste of Gold nanoparticles and Multi-walled Carbon Nanotubes on a gold wire. The acetylcholinesterase enzyme was immobilized on carboxylated Single-walled Carbon Nanotubes and pasted onto a core of electrode followed by coating with a nafion layer to prevent enzyme leaching from the electrode. This electrode was further used as a working electrode in the sensor. This sensor worked on the AChE inhibition mechanism where the signal is inversely proportional to the amount of organophosphorous compounds. The electrocatalytic activity of this sensor was observed at a potential of +0.360 mV. The standardized conditions for this sensor were pH at 7.0, temperature at 30°C and response time at less than 10s. The linear working range of this biosensor was 0.1-130 µM with the lowest detection limit (LOD) of 1.9, 2.3, 2.2 and 2.5 nM for Methyl Parathion, Monocrotophos, Chlorpyrifos and Endosulfan, respectively. The biosensor showed excellent reusability (upto 55 times) and can be stored stably for 2 months.
Topics: Biosensing Techniques; Electrodes; Enzymes, Immobilized; Fluorocarbon Polymers; Gold; Metal Nanoparticles; Nanotubes, Carbon
PubMed: 30052145
DOI: 10.1080/09593330.2018.1505964 -
Toxicology and Industrial Health Dec 2017This study aims to evaluate the genotoxic potential of four commercial pesticides with diverse health categorizations by different world associations currently in use....
This study aims to evaluate the genotoxic potential of four commercial pesticides with diverse health categorizations by different world associations currently in use. We tested the fungicide mancozeb and the insecticides pirimicarb, monocrotophos and permethrin. The research was done with in vitro human peripheral blood lymphocytes using the DNA single gel electrophoresis assay and the cytokinesis-block micronucleus (MN) test, where we analysed common parameters such as the tail moment and the frequency of MN formation. We also measured other parameters like frequency of nucleoplasmic bridges, nuclear buds, apoptosis and necrosis with the MN test. Each pesticide induced significant differences in all of these parameters when compared with the negative control and showed different behaviours in the concentration-dependent response. This could be attributed to their genotoxic potential where mancozeb and monocrotophos induced the highest genetic damage, permethrin caused mainly cell death and pirimicarb had the least impact upon cells. This research provides valuable data about the harmful effects of these pesticides on human cells and may be an important contribution in the construction of a unique international classification of health and to reinforce the use of genotoxic analyses to regulate the use of pesticides.
Topics: Cells, Cultured; Comet Assay; DNA; Humans; Leukocytes, Mononuclear; Micronucleus Tests; Mutagens; Pesticides
PubMed: 29137571
DOI: 10.1177/0748233717736121 -
Clinical Toxicology (Philadelphia, Pa.) 2015Monocrotophos, implicated in about 1/4th of organophosphate poisonings in our centre, is associated with the highest mortality (24%). Yet data on its pharmacokinetics in...
INTRODUCTION
Monocrotophos, implicated in about 1/4th of organophosphate poisonings in our centre, is associated with the highest mortality (24%). Yet data on its pharmacokinetics in humans is limited. We estimated the renal elimination half-life of monocrotophos.
PATIENTS AND METHODS
Consecutive patients presenting with monocrotophos overdose over a 2-month period who had normal renal function were recruited. Monocrotophos in plasma and urine were quantitated by high-performance liquid chromatography. Urine was obtained from catheterised samples at 0-2, 2-4, 4-6, 6-8, 8-12 and 12-24 h. Plasma specimens were collected at the time of admission, and at the midpoint of the urine sample collections at 1, 3, 5, 7, 10, 15 and 21 h. Renal elimination half-life was calculated from the cumulative amount excreted in the urine.
RESULTS
The cohort of 5 male patients, aged 35.8 ± 2.94 years, presented with typical organophosphate (cholinergic) toxidrome following intentional monocrotophos overdose. All patients required mechanical ventilation; one patient died. Plasma data was available from 5 patients and urine data from 3 patients. The median renal elimination half-life was 3.3 (range: 1.9-5.0 h). Plasma monocrotophos values, as natural log, fell in a linear fashion up to around 10 h after admission. After the 10-hour period, there was a secondary rise in values in all the 3 patients in whom sampling was continued after 10 h.
CONCLUSION
A renal elimination half-life of 3.3 h for monocrotophos is consistent with a water-soluble compound which is rapidly cleared from the plasma. The secondary rise in plasma monocrotophos values suggests possible re-distribution. Determining the elimination profile of this compound will help develop better strategies for treatment.
Topics: Adult; Chromatography, High Pressure Liquid; Half-Life; Humans; Intensive Care Units; Kidney; Male; Monocrotophos; Organophosphate Poisoning; Renal Elimination; Specimen Handling
PubMed: 26065438
DOI: 10.3109/15563650.2015.1054500 -
Journal of Hazardous Materials Aug 2024Organophosphorus pesticides (OPPs) are important chemical stressors in aquatic ecosystems, and they attract increasing more attentions recently. However, the impacts of...
Organophosphorus pesticides (OPPs) are important chemical stressors in aquatic ecosystems, and they attract increasing more attentions recently. However, the impacts of different OPPs on carbon cycling remain unclear, particularly for those functional-yet-uncultivable microbes. This study investigated the change in lake aquatic microbial communities in the presence of dichlorvos, monocrotophos, omethoate and parathion. All OPPs significantly inhibited biomass (p < 0.05) and the expression of carbon cycle-related cbbLG gene (p < 0.01), and altered aquatic microbial community structure, interaction, and assembly. Variance partitioning analysis showed a stronger impact of pesticide type on microbial biomass and community structure, where pesticide concentration played more significant roles in carbon cycling. From analysis of cbbLG gene and PICRUSt2, Luteolibacter and Verrucomicrobiaceae assimilated inorganic carbon through Wood-Ljungdahl pathway, whereas it was Calvin-Benson-Bassham cycle for Cyanobium PCC-6307. This work provides a deeper insight into the behavior and mechanisms of microbial community change in aquatic system in response to OPPs, and explicitly unravels the impacts of OPPs on their carbon-cycling functions.
Topics: Pesticides; Bacteria; Water Pollutants, Chemical; Carbon Cycle; Water Microbiology; Lakes; Microbiota; Organophosphorus Compounds; Biomass; Monocrotophos
PubMed: 38876013
DOI: 10.1016/j.jhazmat.2024.134824 -
Environmental Research Jun 2024The existence of multiple pesticide residues in fruits and vegetables constitutes a direct peril to living organisms. Therefore, it is crucial to develop a low-cost...
The existence of multiple pesticide residues in fruits and vegetables constitutes a direct peril to living organisms. Therefore, it is crucial to develop a low-cost screening method for determining organophosphate pesticides (OPPs) in food samples. This study describes the solvothermal synthesis of a ternary composite comprising multi-walled carbon nanotubes (MWCNT), zirconium oxide, and a zirconium-metal-organic framework (Zr-MOF). The ternary composite was characterised using XRD, FESEM, FTIR, and BET. The ternary composite provides a large surface area (1158 m/g) compared with the pristine Zr-MOF (868 m/g). The composite-modified glassy carbon electrode was used to determine nine pesticides, including organophosphate (malathion, dimethoate, chlorpyrifos, monocrotophos, and glyphosate) and non-organophosphate (thiophanate methyl, carbendazim, atrazine, and 2,4, D). In particular, various chemical combinations of OPPs were selected, such as S-P=S, P=S, P=O, and non-OPPs such as C=S (with sulphur), and without sulphur. The sensor results show that the sensor selectivity is high for OPPs containing both phosphorus and sulphur molecules. The low detection limit of the sensor was 2.02, 2.8, 2.5, 1.11, and 2.01 nM for malathion, chlorpyrifos, dimethoate, monocrotophos, and glyphosate, respectively. The electrode exhibited significant chemical stability (93%) after 100 cycles, good repeatability, and a long shelf life. The sensor is reliable for qualitative real-time applications.
Topics: Zirconium; Pesticides; Nanotubes, Carbon; Electrochemical Techniques; Organophosphorus Compounds
PubMed: 38462090
DOI: 10.1016/j.envres.2024.118648 -
Analytical and Bioanalytical Chemistry Sep 2014The present study on in vitro formation and characterization of lysozyme adduct with monocrotophos (MP) evaluates the potential of lysozyme to be used as a sensitive...
The present study on in vitro formation and characterization of lysozyme adduct with monocrotophos (MP) evaluates the potential of lysozyme to be used as a sensitive biomarker to monitor exposure levels to the commonly used organophosphorus pesticide monocrotophos. Crystallization of lysozyme protein adduct with monocrotophos was also undertaken to understand the adduct formation mechanism at a molecular level. The binding of organophosphorus pesticides to lysozyme is one of the key steps in their mutagenicity. The formation and structural characterization of lysozyme adduct with monocrotophos was done using MALDI-TOFMS, fluorescence, UV/Vis spectroscopy, circular dichroism, and X-ray diffraction studies. We report the crystal structure of lysozyme adduct with monocrotophos at 1.9 Å. It crystallized in the P43 space group with two monomers in one asymmetric unit having one molecule of monocrotophos bound to each protein chain. The results proved that the fluorescence quenching of lysozyme by monocrotophos is due to binding of monocrotophos with a tryptophan residue of lysozyme. Monocrotophos interacts most strongly with the Trp-108 and Asp-52 of lysozyme. The interactions of the monocrotophos molecule with the lysozyme suggest the formation of a stable adduct. In addition, the alteration of lysozyme secondary structure in the presence of monocrotophos was confirmed by circular dichroism and fluorescence inhibition of lysozyme by increasing monocrotophos and UV/Vis spectrophotometry. The formation of lysozyme adduct with monocrotophos was confirmed by MALDI-TOFMS.
Topics: Animals; Binding Sites; Biomarkers; Buffers; Chickens; Circular Dichroism; Humans; Hydrogen-Ion Concentration; Monocrotophos; Muramidase; Pesticides; Protein Binding; Protein Structure, Secondary; Spectrometry, Fluorescence; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Ultraviolet; Tryptophan; X-Ray Diffraction
PubMed: 24969463
DOI: 10.1007/s00216-014-7953-y