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The Journal of Foot and Ankle Surgery :... 2017Osteomyelitis due to Ochrobactrum anthropi, a new genus Ochrobacterum widely distributed in the environment and occasionally associated with human infection, has been... (Review)
Review
Osteomyelitis due to Ochrobactrum anthropi, a new genus Ochrobacterum widely distributed in the environment and occasionally associated with human infection, has been described in only a few case reports. We present a report of an unusual case of osteomyelitis caused by O. anthropi that was identified 9 years after a nail puncture to the lateral cuneiform bone. The patient was an 18-year-old male with a painful foot lesion that had originally been misdiagnosed as an osteolytic tumor. He underwent surgery and 2 firm pieces of rubber measuring 7 and 10 mm were removed from the lower portion of the lateral cuneiform bone, which appeared to be affected by an infection. After surgical debridement, O. anthropi was isolated from the bone cultures. The patient was successfully treated with a 6-week course of oral ciprofloxacin and clindamycin. At 1 year after the corrected diagnosis and appropriate treatment, he was symptom free and had resumed regular activities and an athletic lifestyle.
Topics: Adolescent; Anti-Bacterial Agents; Bone Neoplasms; Ciprofloxacin; Clindamycin; Debridement; Diagnosis, Differential; Gram-Negative Bacterial Infections; Humans; Male; Ochrobactrum anthropi; Osteomyelitis; Wounds, Penetrating
PubMed: 28633790
DOI: 10.1053/j.jfas.2017.02.008 -
3 Biotech Feb 2019An bacterial strain named as NC-1, capable of utilizing phenmedipham (PMP) herbicide as the sole of carbon source and energy for growth was isolated from...
An bacterial strain named as NC-1, capable of utilizing phenmedipham (PMP) herbicide as the sole of carbon source and energy for growth was isolated from pesticide-contaminated soil sample by enrichment culture technique. The isolated bacterial strain was identified as NC-1 (MH 796134) based on its morphological, cultural, biochemical characteristics and analysis of 16S rRNA gene sequence. The strain NC-1 could degrade more than 98.5% of PMP (2 mM) within 168 h. The optimal degradation pH and temperature were 7.0 and 30-35 °C, respectively. The strain NC-1 degraded PMP by a pathway involving its initial hydrolysis of their central amide carbamate linkage to yield -aminophenol via methyl--(3-hydroxyphenyl) carbamate and -toluidine were the major intermediates. However, -aminophenol was not further metabolized, because they neither supported the growth of organism nor stimulated oxygen uptake. But -toluidine released by dealkylation was followed by hydrolysis. Further, results also revealed that degradation of 4-methyl catechol proceeded via 2-hydroxy-5-methyl-6-oxohexa-2, 4-dienoate through cleavage ring processes. The formation of these compounds was confirmed by UV, TLC, HPLC, IR, NMR, and GC-MS spectral analysis. The cell-free extracts of NC-1 grown on PMP contained the activities of PMP hydrolase, toluidine dioxygenase, and 4-methyl catechol 1, 2-dioxygenase. These results demonstrate the biodegradation of PMP and promote the potential use of strain NC-1 to bioremediate PMP-contaminated environment.
PubMed: 30729076
DOI: 10.1007/s13205-019-1589-8 -
Carbohydrate Polymers Oct 2023Lipopolysaccharides (LPS) are major players in bacterial infection through the recognition by Toll-like receptor 4 (TLR4). The LPS chemical structure, including the...
Lipopolysaccharides (LPS) are major players in bacterial infection through the recognition by Toll-like receptor 4 (TLR4). The LPS chemical structure, including the oligosaccharide core and the lipid A moiety, can be strongly influenced by adaptation and modulated to assure bacteria protection, evade immune surveillance, or reduce host immune responses. Deep structural understanding of TLRs signaling is essential for the modulation of the innate immune system in sepsis control and inflammation, during bacterial infection. To advance this knowledge, we have employed computational techniques to characterize the TLR4 molecular recognition of atypical LPSs from different opportunistic members of α2-Proteobacteria, including Brucella melitensis, Ochrobactrum anthropi, and Ochrobactrum intermedium, with diverse immunostimulatory activities. We contribute to unraveling the role of uncommon lipid A chemical features such as bearing very long-chain fatty acid chains, whose presence has been rarely reported, on modulating the proper heterodimerization of the TLR4 receptor complex. Moreover, we further evaluated the influence of the different oligosaccharide cores, including sugar composition and net charge, on TLR4 activation. Our studies contribute to elucidating, from the molecular and biological perspectives, the impact of the α2-Proteobacteria LPS cores and the chemical structure of the atypical lipid A for immune system evasion in opportunistic bacteria.
Topics: Humans; Lipopolysaccharides; Toll-Like Receptor 4; Lipid A; Proteobacteria; Immune Evasion; Bacteria; Bacterial Infections; Oligosaccharides
PubMed: 37479429
DOI: 10.1016/j.carbpol.2023.121094 -
New Microbes and New Infections Nov 2018a rare human pathogen, has been isolated predominantly from patients with catheter-related bacteraemia and rarely from other infections. In 2016, six cases of...
a rare human pathogen, has been isolated predominantly from patients with catheter-related bacteraemia and rarely from other infections. In 2016, six cases of pseudo-bacteraemia caused by carbapenem-resistant isolates were recovered from an Argentinian hospital. The resistant phenotype exposed by the isolates caught our attention and led to an extensive epidemiologic investigation. Here we describe the characterization of a carbapenem-resistant outbreak whose probable cause was by contaminated collection tubes. The genome analysis of one strain revealed the presence of various resistant determinants. Among them, a metal-dependent hydrolase of the β-lactamase superfamily I, was found. Lately the recovery of unusual multidrug-resistant pathogens in the clinical setting has increased, thus emphasizing the need to implement standardized infection control practice and epidemiologic investigation to identify the real cause of hospital outbreaks.
PubMed: 30345061
DOI: 10.1016/j.nmni.2018.09.002 -
Proteomics Dec 2016Ochrobactrum anthropi is a Gram-negative rod belonging to the Brucellaceae family, able to colonize a variety of environments, and actually reported as a human...
Ochrobactrum anthropi is a Gram-negative rod belonging to the Brucellaceae family, able to colonize a variety of environments, and actually reported as a human opportunistic pathogen. Despite its low virulence, the bacterium causes a growing number of hospital-acquired infections mainly, but not exclusively, in immunocompromised patients. The aim of this study was to obtain an overview of the global proteome changes occurring in O. anthropi in response to different growth temperatures, in order to achieve a major understanding of the mechanisms by which the bacterium adapts to different habitats and to identify some potential virulence factors. Combined quantitative mass spectrometry-based proteomics and bioinformatics approaches were carried out on two O. anthropi strains grown at temperatures miming soil/plants habitat (25°C) and human host environment (37°C), respectively. Proteomic analysis led to the identification of over 150 differentially expressed proteins in both strains, out of over 1200 total protein identifications. Among them, proteins responsible for heat shock response (DnaK, GrpE), motility (FliC, FlgG, FlgE), and putative virulence factors (TolB) were identified. The study represents the first quantitative proteomic analysis of O. anthropi performed by high-resolution quantitative mass spectrometry.
Topics: Bacterial Proteins; Ecosystem; Host-Pathogen Interactions; Humans; Ochrobactrum anthropi; Temperature; Virulence Factors
PubMed: 27753207
DOI: 10.1002/pmic.201600048 -
Archives of Microbiology Jul 2017Four bacterial strains from glyphosate- or alkylphosphonates-contaminated soils were tested for ability to utilize different organophosphonates. All studied strains...
Four bacterial strains from glyphosate- or alkylphosphonates-contaminated soils were tested for ability to utilize different organophosphonates. All studied strains readily utilized methylphosphonic acid and a number of other phosphonates, but differed in their ability to degrade glyphosate. Only strains Ochrobactrum anthropi GPK 3 and Achromobacter sp. Kg 16 utilized this compound after isolation from enrichment cultures with glyphosate. Achromobacter sp. MPK 7 from the same enrichment culture, similar to Achromobacter sp. MPS 12 from methylphosphonate-polluted source, required adaptation to growth on GP. Studied strains varied significantly in their growth parameters, efficiency of phosphonates degradation and characteristic products of this process, as well as in their energy metabolism. These differences give grounds to propose a possible model of interaction between these strains in microbial consortium in phosphonate-contaminated soils.
Topics: Achromobacter; Biodegradation, Environmental; Glycine; Microbial Consortia; Ochrobactrum anthropi; Organophosphonates; Organophosphorus Compounds; Soil; Soil Microbiology; Soil Pollutants; Glyphosate
PubMed: 28184965
DOI: 10.1007/s00203-017-1343-8 -
Applied Biochemistry and Biotechnology Jan 2015Fluoranthene and pyrene are polycyclic aromatic hydrocarbons of high molecular weight that are recalcitrant and toxic to humans; therefore, their removal from the...
Fluoranthene and pyrene are polycyclic aromatic hydrocarbons of high molecular weight that are recalcitrant and toxic to humans; therefore, their removal from the environment is crucial. From hydrocarbon-contaminated soil, 25 bacteria and 12 filamentous fungi capable of growth on pyrene and fluoranthene as the sole carbon and energy source were isolated. From these isolates, Ochrobactrum anthropi BPyF3 and Fusarium sp. FPyF1 were selected and identified because they grew quickly and abundantly in both hydrocarbons. Furthermore, O. anthropi BPyF3 and Fusarium sp. FPyF1 were most efficient at removing pyrene (50.39 and 51.32 %, respectively) and fluoranthene (49.85 and 49.36 %, respectively) from an initial concentration of 50 mg L(-1) after 7 days of incubation. Based on this and on the fact that there was no antagonism between the two microorganisms, a coculture composed of O. anthropi BPyF3 and Fusarium sp. FPyF1 was formed to remove fluoranthene and pyrene at an initial concentration of 100 mg L(-1) in a removal kinetic assay during 21 days. Fluoranthene removal by the coculture was higher (87.95 %) compared with removal from the individual cultures (68.95 % for Fusarium sp. FPyF1 and 64.59 % for O. anthropi BPyF3). In contrast, pyrene removal by the coculture (99.68 %) was similar to that obtained by the pure culture of Fusarium sp. FPyF1 (99.75 %). The kinetics of removal for both compounds was adjusted to a first-order model. This work demonstrates that the coculture formed by Fusarium sp. FPyF1 and O. anthropi BPyF3 has greater potential to remove fluoranthene than individual cultures; however, pyrene can be removed efficiently by Fusarium sp. FPyF1 alone.
Topics: Biodegradation, Environmental; Coculture Techniques; Culture Media; Environmental Pollutants; Fluorenes; Fusarium; Kinetics; Ochrobactrum anthropi; Pyrenes
PubMed: 25369894
DOI: 10.1007/s12010-014-1336-x -
International Journal of Systematic and... Aug 2019The genus Ochrobactrum belongs to the family Brucellaceae and its members are known to be adapted to a wide range of ecological niches. Ochrobactrum anthropi ATCC 49188...
The genus Ochrobactrum belongs to the family Brucellaceae and its members are known to be adapted to a wide range of ecological niches. Ochrobactrum anthropi ATCC 49188 and Ochrobactrum lupini LUP21 are strains isolated from human clinical and plant root nodule samples, respectively, which share high similarity for phylogenetic markers (i.e 100 % for 16S rRNA, 99.9 % for dnaK and 99.35 % for rpoB). In this work, multiple genome average nucleotide identity (ANI) approaches, digital DNA-DNA hybridization (dDDH) and phylogenetic analysis were performed in order to investigate the taxonomic relationship between O. anthropi ATCC 49188, O. lupini LUP21, and other five type strains from the genus Ochrobactrum. Whole-genome comparisons demonstrated that O. lupini LUP21 and the Ochrobactrum genus type species, O. anthropi ATCC 49188, share 97.55 % of ANIb, 98.25 % of ANIm, 97.99 % of gANI, 97.94 % of OrthoANI and 83.9 % of dDDH, which exceed the species delineation thresholds. These strains are also closely related in phylogenies reconstructed from a concatenation of 1193 sequences from single-copy ortholog genes. A review of their profiles revealed that O. anthropi ATCC 49188 and O. lupini LUP21 do not present pronounced differences at phenotypic and chemotaxonomic levels. Considering phylogenetic, genomic, phenotypic and chemotaxonomic data, O. lupini should be considered a later heterotypic synonym of O. anthropi.
Topics: Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Humans; Nucleic Acid Hybridization; Ochrobactrum; Ochrobactrum anthropi; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 31120414
DOI: 10.1099/ijsem.0.003465 -
Journal of Laboratory Physicians Mar 2023, due to its robust survival abilities, has been known to cause nosocomial and opportunistic infections, posing both diagnostic and therapeutic challenges. Low...
, due to its robust survival abilities, has been known to cause nosocomial and opportunistic infections, posing both diagnostic and therapeutic challenges. Low virulence, indolent clinical presentation, and lack of awareness on their clinical significance attribute to the underreporting of the same. We report two cases of bacteremia in oncology patients presented to us in a short span of 6 months, which indicates that such infections might be quite common in immunocompromised hosts. Both our strains were susceptible to carbapenems, trimethoprim/sulfamethoxazole, and minocycline, and recovered with monotherapy. More vigilant and accurate diagnostic techniques need to be followed not to miss such pathogens. Early identification and administration of appropriate antibiotics have been associated with a good outcome.
PubMed: 37064972
DOI: 10.1055/s-0042-1757235 -
BMC Biotechnology Sep 2021The unnatural amino acid, L-2-aminobutyric acid (L-ABA) is an essential chiral building block for various pharmaceutical drugs, such as the antiepileptic drug...
BACKGROUND
The unnatural amino acid, L-2-aminobutyric acid (L-ABA) is an essential chiral building block for various pharmaceutical drugs, such as the antiepileptic drug levetiracetam and the antituberculosis drug ethambutol. The present study aims at obtaining variants of ω-transaminase from Ochrobactrum anthropi (OATA) with high catalytic activity to α-ketobutyric acid through protein engineering.
RESULTS
Based on the docking model using α-ketobutyric acid as the ligand, 6 amino acid residues, consisting of Y20, L57, W58, G229, A230 and M419, were chosen for saturation mutagenesis. The results indicated that L57C, M419I, and A230S substitutions demonstrated the highest elevation of enzymatic activity among 114 variants. Subsequently, double substitutions combining L57C and M419I caused a further increase of the catalytic efficiency to 3.2-fold. This variant was applied for threonine deaminase/OATA coupled reaction in a 50-mL reaction system with 300 mM L-threonine as the substrate. The reaction was finished in 12 h and the conversion efficiency of L-threonine into L-ABA was 94%. The purity of L-ABA is 75%, > 99% ee. The yield of L-ABA was 1.15 g.
CONCLUSION
This study provides a basis for further engineering of ω-transaminase for producing chiral amines from keto acids substrates.
Topics: Aminobutyrates; Catalytic Domain; Ochrobactrum anthropi; Transaminases
PubMed: 34563172
DOI: 10.1186/s12896-021-00713-7