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Journal of Global Antimicrobial... Jun 2022To explore the genomic characterization of an IMP-8-producing Ochrobactrum anthropic and give suggestions for the application of antibiotics.
OBEJECTIVES
To explore the genomic characterization of an IMP-8-producing Ochrobactrum anthropic and give suggestions for the application of antibiotics.
METHODS
In 2021, the infection caused by CRKP was under control after nearly three months of using CAV, however, carbapenem-resistant O. anthropi isolates were collected from a rectal swab sample of a patient with Lumbar Disc Herniation Postoperative Infection. The rectal swab was then enriched in lysogeny broth overnight and inoculated onto China Blue agar plates containing 0.3µg/mL meropenem. And we investigated the characteristics of this carbapenem-resistant O. anthropi by MALDI-TOF MS, Immune colloidal gold technique, conjugation experiment, whole genome sequencing and antimicrobial susceptibility testing.
RESULTS
Antimicrobial susceptibility testing showed that the O. anthropi were resistant to imipenem, cefmetazole, ceftazidime, cefotaxime, piperacillin/tazobactam, sulbactam/cefopcrazone, ceftazidime/avibactam, cefepime, ciprofloxacin, aztreonam, and not susceptible to meropenem, ertapenem, polymyxin B, tigecycline, amikacin. Immune colloidal gold technique reflected that this strain produced IMP carbapenemases, and the presence of IMP-8 was verified by WGS, which was located in a 21,442 bp, nonconjugative plasmid.
CONCLUSION
Improper antibiotic treatment can cause intestinal flora imbalance and even bacteremia in patients, we should use antibiotics wisely and develop individualized treatment options.
Topics: Anti-Bacterial Agents; Carbapenems; Ceftazidime; Gold Colloid; Humans; Meropenem; Microbial Sensitivity Tests; Ochrobactrum anthropi; beta-Lactamases
PubMed: 35346886
DOI: 10.1016/j.jgar.2022.03.016 -
Revista Chilena de Infectologia :... Aug 2018
Topics: Anti-Bacterial Agents; Culture Media; Ochrobactrum anthropi
PubMed: 30534931
DOI: 10.4067/s0716-10182018000400431 -
Infectious Disease Reports Feb 2010Ochrobactrum anthropi is a rare cause of orthopedic infections. We report the second case of Ochrobactrum anthropi septic arthritis in the literature. Our case... (Review)
Review
Ochrobactrum anthropi is a rare cause of orthopedic infections. We report the second case of Ochrobactrum anthropi septic arthritis in the literature. Our case highlights the ability of Ochrobactrum anthropi to cause septic arthritis and its relevance in the field of orthopedic infections.
PubMed: 24470885
DOI: 10.4081/idr.2010.e2 -
Materials (Basel, Switzerland) Oct 2018Epoxy resins have a wide range of applications, including in corrosion protection of metals, electronics, structural adhesives, and composites. The consumption of epoxy...
Epoxy resins have a wide range of applications, including in corrosion protection of metals, electronics, structural adhesives, and composites. The consumption of epoxy resins is predicted to keep growing in the coming years. Unfortunately, thermoset resins cannot be recycled, and are typically not biodegradable. Hence, they pose environmental pollution risk. Here, we report degradation of epoxy resin by two bacteria that are capable of using epoxy resin as a sole carbon source. These bacteria were isolated from soil samples collected from areas around an epoxy and polyurethanes manufacturing plant. Using an array of molecular, biochemical, analytical, and microscopic techniques, they were identified as and . As epoxy was the only carbon source available for these bacteria, their measured growth rate reflected their ability to degrade epoxy resin. Bacterial growth took place only when the two bacteria were grown together, indicating a synergistic effect. The surface morphology of the epoxy droplets changed significantly due to the biodegradation process. The metabolic pathway of epoxy by these two microbes was investigated by liquid chromatography mass spectrometry. Bisphenol A, 3,3'-((propane-2,2-diylbis(4,1-phenylene))bis(oxy))bis(propane-1,2-diol) and some other constituents were identified as being consumed by the bacteria.
PubMed: 30380643
DOI: 10.3390/ma11112123 -
Case Reports in Ophthalmological... 2016. To describe a unique case of keratitis associated with a rare manifestation of Descemet's membrane detachment and intracorneal hypopyon and to discuss challenges in...
. To describe a unique case of keratitis associated with a rare manifestation of Descemet's membrane detachment and intracorneal hypopyon and to discuss challenges in diagnosis and management. . Best-corrected visual acuity was measured with Snellen letters. Corneal scrapings were performed and aerobic, viral, herpetic, acid-fast bacilli, and fungal stains and cultures were obtained. Following evisceration, tissue was evaluated for histologic features and again stained for bacteria, mycobacteria, , fungi, and viral particles. . Initial presentation to our institute was notable for a corneal ulcer, focal Descemet's membrane detachment, and intracorneal hypopyon. Speciation of initial corneal scrapes revealed and initial management included fortified tobramycin. Despite medical therapy, the patient developed a corneal perforation and required subsequent evisceration. . is an emerging ocular pathogen that has not been previously reported in cases of keratitis. As this pathogen becomes increasingly recognized as a source of ocular infections, it is important to identify and treat aggressively to avoid vision-threatening disease.
PubMed: 27777806
DOI: 10.1155/2016/4502105 -
Journal of Hand Surgery Global Online Nov 2020is an opportunistic, low-virulence pathogen occasionally associated with human infections and found largely in immunocompromised patients and those with intravascular...
is an opportunistic, low-virulence pathogen occasionally associated with human infections and found largely in immunocompromised patients and those with intravascular devices. We report the case of a healthy 70-year-old man who presented with an infection of the hand, who had no history of trauma but had been gardening for 4 months. Despite surgical debridement and empirical antibiotics, the infection could not be controlled. Cultures revealed . Antibiotic treatment was adapted to intravenous cefepime for 15 days and the infection was finally controlled after a second surgery. Oral cotrimoxazole was continued for another 2 weeks. infection of the hand must be considered not only in immunosuppressed patients but also in healthy patients without intravascular devices. Local debridement and empiric antibiotic may be insufficient. Antibiotic therapy should follow susceptibility testing, but usually includes a broad-spectrum intravenous beta-lactam such as imipenem-cilastatin or cefepime, or oral cotrimoxazole or ciprofloxacin.
PubMed: 35415523
DOI: 10.1016/j.jhsg.2020.08.006 -
Journal of Orthopaedic Case Reports Mar 2022Ochrobactrum anthropi is an opportunistic and rare human pathogen, which is seen widely in the environment. O. anthropi infections have been reported in both...
INTRODUCTION
Ochrobactrum anthropi is an opportunistic and rare human pathogen, which is seen widely in the environment. O. anthropi infections have been reported in both immunocompetent and immunocompromised individuals. There is no proper consensus on the diagnosis and management of O. anthropi related infections.
CASE REPORT
We report a case of O. anthropi related left distal clavicular osteomyelitis in an immunocompetent individual with an elaborative diagnostic and treatment algorithm for its effective management.
CONCLUSION
A comprehensive management strategy with a combination of implant removal (if present) with extensive surgical debridement of bone and soft tissue and intravenous antibiotics results in successful eradication of O. anthropi infection.
PubMed: 36199934
DOI: 10.13107/jocr.2022.v12.i03.2730 -
Microorganisms Apr 2020Azoxystrobin is one of the most popular strobilurin fungicides, widely used in agricultural fields for decades.Extensive use of azoxystrobin poses a major threat to...
Azoxystrobin is one of the most popular strobilurin fungicides, widely used in agricultural fields for decades.Extensive use of azoxystrobin poses a major threat to ecosystems. However, little is known about the kinetics and mechanism of azoxystrobin biodegradation. The present study reports a newly isolated bacterial strain, SH14, utilizing azoxystrobin as a sole carbon source, was isolated from contaminated soils. Strain SH14 degraded 86.3% of azoxystrobin (50 μg·mL) in a mineral salt medium within five days. Maximum specific degradation rate (), half-saturation constant (), and inhibition constant () were noted as 0.6122 d, 6.8291 μg·mL, and 188.4680 μg·mL, respectively.Conditions for strain SH14 based azoxystrobin degradation were optimized by response surface methodology. Optimum degradation was determined to be 30.2 °C, pH 7.9, and 1.1 × 10 CFU·mL of inoculum. Strain SH14 degraded azoxystrobin via a novel metabolic pathway with the formation of -(4,6-dimethoxypyrimidin-2-yl)-acetamide,2-amino-4-(4-chlorophenyl)-3-cyano-5,6-dimethyl-pyridine, and 3-quinolinecarboxylic acid,6,8-difluoro-4-hydroxy-ethyl ester as the main intermediate products, which were further transformed without any persistent accumulative product. This is the first report of azoxystrobin degradation pathway in a microorganism. Strain SH14 also degraded other strobilurin fungicides, including kresoxim-methyl (89.4%), pyraclostrobin (88.5%), trifloxystrobin (78.7%), picoxystrobin (76.6%), and fluoxastrobin (57.2%) by following first-order kinetic model. Bioaugmentation of azoxystrobin-contaminated soils with strain SH14 remarkably enhanced the degradation of azoxystrobin, and its half-life was substantially reduced by 95.7 and 65.6 days in sterile and non-sterile soils, respectively, in comparison with the controls without strain SH14. The study presents SH14 for enhanced biodegradation of azoxystrobin and elaborates on the metabolic pathways to eliminate its residual toxicity from the environment.
PubMed: 32357564
DOI: 10.3390/microorganisms8050625 -
3 Biotech Feb 2019An bacterial strain named as NC-1, capable of utilizing phenmedipham (PMP) herbicide as the sole of carbon source and energy for growth was isolated from...
An bacterial strain named as NC-1, capable of utilizing phenmedipham (PMP) herbicide as the sole of carbon source and energy for growth was isolated from pesticide-contaminated soil sample by enrichment culture technique. The isolated bacterial strain was identified as NC-1 (MH 796134) based on its morphological, cultural, biochemical characteristics and analysis of 16S rRNA gene sequence. The strain NC-1 could degrade more than 98.5% of PMP (2 mM) within 168 h. The optimal degradation pH and temperature were 7.0 and 30-35 °C, respectively. The strain NC-1 degraded PMP by a pathway involving its initial hydrolysis of their central amide carbamate linkage to yield -aminophenol via methyl--(3-hydroxyphenyl) carbamate and -toluidine were the major intermediates. However, -aminophenol was not further metabolized, because they neither supported the growth of organism nor stimulated oxygen uptake. But -toluidine released by dealkylation was followed by hydrolysis. Further, results also revealed that degradation of 4-methyl catechol proceeded via 2-hydroxy-5-methyl-6-oxohexa-2, 4-dienoate through cleavage ring processes. The formation of these compounds was confirmed by UV, TLC, HPLC, IR, NMR, and GC-MS spectral analysis. The cell-free extracts of NC-1 grown on PMP contained the activities of PMP hydrolase, toluidine dioxygenase, and 4-methyl catechol 1, 2-dioxygenase. These results demonstrate the biodegradation of PMP and promote the potential use of strain NC-1 to bioremediate PMP-contaminated environment.
PubMed: 30729076
DOI: 10.1007/s13205-019-1589-8 -
New Microbes and New Infections Nov 2018a rare human pathogen, has been isolated predominantly from patients with catheter-related bacteraemia and rarely from other infections. In 2016, six cases of...
a rare human pathogen, has been isolated predominantly from patients with catheter-related bacteraemia and rarely from other infections. In 2016, six cases of pseudo-bacteraemia caused by carbapenem-resistant isolates were recovered from an Argentinian hospital. The resistant phenotype exposed by the isolates caught our attention and led to an extensive epidemiologic investigation. Here we describe the characterization of a carbapenem-resistant outbreak whose probable cause was by contaminated collection tubes. The genome analysis of one strain revealed the presence of various resistant determinants. Among them, a metal-dependent hydrolase of the β-lactamase superfamily I, was found. Lately the recovery of unusual multidrug-resistant pathogens in the clinical setting has increased, thus emphasizing the need to implement standardized infection control practice and epidemiologic investigation to identify the real cause of hospital outbreaks.
PubMed: 30345061
DOI: 10.1016/j.nmni.2018.09.002