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Chemosphere Jan 2020Ochrobactrum anthropi DE2010 is a microorganism isolated from Ebro Delta microbial mats and able to resist high doses of chromium(III) due to its capacity to tolerate,...
Ochrobactrum anthropi DE2010 is a microorganism isolated from Ebro Delta microbial mats and able to resist high doses of chromium(III) due to its capacity to tolerate, absorb and accumulate this metal. The effect of this pollutant on O. anthropi DE2010 has been studied assessing changes in viability and biomass, sorption yields and removal efficiencies. Furthermore, and for the first time, its capacity for immobilizing Cr(III) from culture media was tested by a combination of High Angle Annular Dark Field (HAADF) Scanning Transmission Electron Microscopy (STEM) imaging coupled to Energy Dispersive X-ray spectroscopy (EDX). The results showed that O. anthropi DE2010 was grown optimally at 0-2 mM Cr(III). On the other hand, from 2 to 10 mM Cr(III) microbial plate counts, growth rates, cell viability, and biomass decreased while extracellular polymeric substances (EPS) production increases. Furthermore, this bacterium had a great ability to remove Cr(III) at 10 mM (q = 950.00 mg g) immobilizing it mostly in bright polyphosphate inclusions and secondarily on the cellular surface at the EPS level. Based on these results, O. anthropi DE2010 could be considered as a potential agent for bioremediation in Cr(III) contaminated environments.
Topics: Biodegradation, Environmental; Biomass; Chromium; Microbial Viability; Ochrobactrum anthropi; Spectrometry, X-Ray Emission
PubMed: 31472343
DOI: 10.1016/j.chemosphere.2019.124663 -
Folia Medica Feb 2021The use of central venous catheters for hemodialysis continues to grow worldwide, despite the efforts of many specialists. Patients with end-stage renal disease have... (Review)
Review
The use of central venous catheters for hemodialysis continues to grow worldwide, despite the efforts of many specialists. Patients with end-stage renal disease have impaired immunity, which is why infections are the most common complication seen in them. It worsens their quality of life and is a major cause of high morbidity and mortality, especially in hemodialysis patients.We report two cases of catheter-related bloodstream infection in hemodialysis patients caused by Ochrobactrum anthropi, which are the first reported cases in Bulgaria and present a brief literature review of the known facts.
Topics: Aged; Bacteremia; Bulgaria; Catheter-Related Infections; Catheters; Gram-Negative Bacterial Infections; Humans; Kidney Failure, Chronic; Male; Middle Aged; Ochrobactrum anthropi; Renal Dialysis
PubMed: 33650409
DOI: 10.3897/folmed.63.e53131 -
Ochrobactrum anthropi: An emerging pathogen causing meningitis with sepsis in a neurotrauma patient.Journal of Infection in Developing... Sep 2017Ochrobactrum anthropi is an unusual emerging pathogen especially in the hospital environment. Most of the reported cases are nosocomially acquired infections in...
Ochrobactrum anthropi is an unusual emerging pathogen especially in the hospital environment. Most of the reported cases are nosocomially acquired infections in patients with various indwelling and invasive medical devices, such as central venous catheters and drainage tubes. We report a case of nosocomially transmitted invasive catheter related septicaemia with meningitis due to O. anthropi, in an elderly immunocompetent male with a head trauma admitted to a level -1 trauma centre. This report describes clinical and microbiological characteristics of rare pathogen and also highlights the importance of rapid identification, susceptibility testing of such opportunistic pathogens in trauma settings and its unique antibiotic susceptibility profiles. This requires prompt treatment with timely intervention, and appropriate antimicrobial therapy, alongside adherence to strict infection control practices.
PubMed: 31600165
DOI: 10.3855/jidc.9146 -
Journal of Clinical Microbiology Jun 2018
Closing the Brief Case: Misidentification of Brucella melitensis as Ochrobactrum anthropi by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).
Topics: Brucella melitensis; Ochrobactrum anthropi; Polymerase Chain Reaction; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 29802239
DOI: 10.1128/JCM.00918-17 -
Cornea May 2021To report a case of Ochrobactrum anthropi keratitis in an eye with a Boston type 1 keratoprosthesis.
PURPOSE
To report a case of Ochrobactrum anthropi keratitis in an eye with a Boston type 1 keratoprosthesis.
METHODS
This is a case report and review of the literature.
RESULTS
A 78-year-old man with a history of implantation of a Boston type 1 keratoprosthesis in the left eye presented for a routine follow-up with no acute complaints. In the left eye, visual acuity was 20/60 and slit-lamp examination revealed a 1.5-mm inferotemporal corneal infiltrate adjacent to the optic stem. Corneal cultures grew abundant O. anthropi. After 7 weeks of topical antimicrobial therapy and placement of a temporary tarsorrhaphy, the keratitis resolved.
CONCLUSIONS
Ochrobactrum anthropi is an organism associated with indwelling medical devices and can be pathogenic in eyes with implanted keratoprostheses.
Topics: Aged; Anti-Bacterial Agents; Artificial Organs; Ceftazidime; Cornea; Corneal Ulcer; Drug Therapy, Combination; Eye Infections, Bacterial; Gram-Negative Bacterial Infections; Humans; Male; Moxifloxacin; Ochrobactrum anthropi; Prostheses and Implants; Slit Lamp Microscopy; Visual Acuity
PubMed: 33252382
DOI: 10.1097/ICO.0000000000002571 -
Clinical Case Reports Jun 2024(. ), a rare opportunistic pathogen, caused sepsis in a malnourished 15-month-old African child. Early detection and appropriate antibiotics led to full recovery,...
KEY CLINICAL MESSAGE
(. ), a rare opportunistic pathogen, caused sepsis in a malnourished 15-month-old African child. Early detection and appropriate antibiotics led to full recovery, highlighting the importance of robust surveillance for emerging pathogens in vulnerable populations.
ABSTRACT
While rarely causing infections, . , a non-fermenting, obligately aerobic, flagellated gram-negative bacillus, demonstrates oxidase positivity and indole negativity. Traditionally, spp is considered a low threat due to its environmental abundance and mild virulence. It is, however, a multidrug-resistant bacteria known for causing opportunistic infections in humans. . is typically associated with catheter-related bloodstream infections. The first documented case was in 1998; most cases have been reported in developed countries. We present a case of . sepsis in a malnourished child in sub-Saharan Africa. We report a case involving a 15-month-old African female who presented with symptoms and signs of protein-energy malnutrition and sepsis. The blood culture revealed .. We treated the child with the empirical first-line antibiotics per the national guidelines, intravenous ampicillin and gentamicin for a week, and the child fully recovered. This report describes a rare case of . sepsis with malnutrition in an African female child. . is an emerging pathogen causing opportunistic infections in both immunocompetent and immunocompromised patients. We report that early bacterial detection, appropriate antibiotic susceptibility and antimicrobial management based on local antibiogram data may be essential for excellent patient outcomes. Additionally, we recommend more robust surveillance to detect such rare emerging pathogens.
PubMed: 38845804
DOI: 10.1002/ccr3.9042 -
Journal of Infection and Public Health 2018Ochrobactrum anthropi has become an emerging pathogen for bloodstream infection (BSI).
BACKGROUND
Ochrobactrum anthropi has become an emerging pathogen for bloodstream infection (BSI).
METHODS
From January 1st 2010 to June 30th 2017, inpatients with one or more blood cultures positive for O. anthropi isolates at Chinese People's Liberation Army General Hospital in Beijing, China, were enrolled in this study. Clinical and laboratory data were collected by reviewing electronic records.
RESULTS
A total of 11 patients with O. anthropi BSI were identified, of which 10 patients survived. There were 6 males and 5 females, whose age ranged from 2 to 83 years. 7 infections were hospital-acquired. In 8 cases O. anthropi was the only pathogen. The most common symptoms of O. anthropi BSI were fever (100%) and disorders of consciousness (45.5%). All patients had undergone indwelling catheter placement. O. anthropi isolates in this study were most susceptible to levofloxacin (100%), ciprofloxacin (85.7%), imipenem (85.7%) and cotrimoxazole (85.7%), while they were widely resistant to penicillins and cephalosporins.
CONCLUSIONS
O. anthropi BSI usually happens in patients with indwelling catheters, and often begins with no distinctive symptom or laboratory finding. O. anthropi seldom form polymicrobial BSIs. Quinolones and carbapenems are optimal antibiotics for O. anthropi BSI. Catheter removal is essential when O. anthropi BSI happens recurrently.
Topics: Adolescent; Adult; Aged; Aged, 80 and over; Anti-Bacterial Agents; Catheter-Related Infections; Child; Child, Preschool; China; Cross Infection; Female; Gram-Negative Bacterial Infections; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Ochrobactrum anthropi; Sepsis; Survival Analysis; Tertiary Care Centers; Young Adult
PubMed: 30100242
DOI: 10.1016/j.jiph.2018.07.009 -
Folia Microbiologica Nov 2019The aim of this work was to compare production of endotoxin and to determine susceptibility to antibiotics in two groups of specimens-wild-type strains Ochrobactrum... (Comparative Study)
Comparative Study
The aim of this work was to compare production of endotoxin and to determine susceptibility to antibiotics in two groups of specimens-wild-type strains Ochrobactrum anthropi isolated from the environment and the strains isolated from patients with cystic fibrosis. The determination of the endotoxin produced by the test strains was carried on by using a limulus amebocyte lysate test (LAL test). Determination of ATB sensitivity was accomplished by means of a broth dilution method in a microtiter plate (MIC). No significant difference was found between the group of ochrobacters isolated from the environment and the group of ochrobacters isolated from cystic fibrosis patients. Antibiotic sensitivity testing has indicated that the resistance to tigecycline, trimethoprim/sulfamethoxazole, and gentamicin was slightly higher in strains isolated from cystic fibrosis patients in comparison with strains isolated from the environment. In general, most of the test strains were sensitive to most of the antibiotics tested. Significant resistance has been demonstrated for cefotaxime. Resistance was also found for gentamicin in strains number 4 and 7. The MIC was equal to the breakpoint for this antibiotic (8000 mg/L).
Topics: Adolescent; Anti-Bacterial Agents; Child; Cystic Fibrosis; Drug Resistance, Bacterial; Endotoxins; Environmental Microbiology; Gram-Negative Bacterial Infections; Humans; Limulus Test; Microbial Sensitivity Tests; Ochrobactrum anthropi
PubMed: 30919306
DOI: 10.1007/s12223-019-00700-8 -
The Science of the Total Environment Feb 2021Previous studies have provided evidence that bioremediation deals a novel approach to graffiti removal, thereby overcoming well-known limitations of current cleaning...
Previous studies have provided evidence that bioremediation deals a novel approach to graffiti removal, thereby overcoming well-known limitations of current cleaning methods. In the present study eight bacteria aerobic, mesophilic and culturable from the American ATCC and the German DSMZ collections of microorganisms, some isolated from car paint waste, colored deposits in a pulp dryer and wastewater from dye works, were tested in the removal of silver and black graffiti spray paints using immersion strategies with glass slides. Absorbance at 600 nm and live/dead assays were performed to estimate bacterial density and activity in all samples. Also, pH and dissolved organic carbon (DOC) and inorganic carbon (DIC) measurements in the liquid media were made, as well as, thickness, colorimetric and infrared (FTIR) spectroscopy measurements in graffiti paint layers were used to evaluate the presence of the selected bacteria in the samples and the graffiti bioremoval capacity of bacteria. Data demonstrated that of the eight bacteria studied, Enterobacter aerogenes, Comamonas sp. and a mixture of Bacillus sp., Delftia lacustris, Sphingobacterium caeni, and Ochrobactrum anthropi were the most promising for bioremoval of graffiti. According to significant changes in FTIR spectra, indicating an alteration of the paint polymeric structure, coupled with the presence of a consistent quantity of live bacteria in the medium as well as a significant increase of DIC (a measure of metabolic activity) and a change in paint color.
Topics: Bacteria; Biodegradation, Environmental; Delftia; Sphingobacterium
PubMed: 33280882
DOI: 10.1016/j.scitotenv.2020.144075 -
Archives of Microbiology Mar 2016A novel Gram-staining negative, motile, rod-shaped and aerobic bacterial strain, designated EGI 60010(T), was isolated from healthy roots of Glycyrrhiza uralensis F....
A novel Gram-staining negative, motile, rod-shaped and aerobic bacterial strain, designated EGI 60010(T), was isolated from healthy roots of Glycyrrhiza uralensis F. collected from Yili County, Xinjiang Province, North-West China. The 16S rRNA gene sequence of strain EGI 60010(T) showed 97.2 % sequence similarities with Ochrobactrum anthropi ATCC 49188(T) and Ochrobactrum cytisi ESC1(T), and 97.1 % with Ochrobactrum lupini LUP21(T). The phylogenetic analysis based on 16S rRNA gene sequences showed that the new isolate clustered with members of the genera Ochrobactrum, and formed a distinct clade in the neighbour-joining tree. Q-10 was identified as the respiratory quinone for strain EGI 60010(T). The major fatty acids were summed feature 8 (C18:1 ω6c and/or C18:1 ω7c), C19:0 cyclo ω8c, summed feature 4 (C17:1 iso I/anteiso B) and C16:0. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol and phosphatidylcholine. The DNA G+C content of strain EGI 60010(T) was determined to be 60.4 mol%. The genomic DNA relatedness values determined between strain EGI 60010(T) and the closely related strains O. anthropi JCM 21032(T), O. cytisi CCTCC AB2014258(T) and O. lupini NBRC 102587(T) were 50.3, 50.0 and 41.6 %, respectively. Based on the results of the molecular studies supported by its differentiating phenotypic characteristics, strain EGI 60010(T) was considered to represent a novel species within the genus Ochrobactrum, for which the name Ochrobactrum endophyticum sp. nov., is proposed. The type strain is EGI 60010(T) (=CGMCC 1.15082(T) = KCTC 42485(T) = DSM 29930(T)).
Topics: Base Composition; China; Fatty Acids; Glycyrrhiza uralensis; Ochrobactrum; Phospholipids; Phylogeny; Plant Roots; Quinones; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Species Specificity
PubMed: 26615404
DOI: 10.1007/s00203-015-1170-8