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BMC Urology Dec 2020Transurethral resection of the prostate (TUR-P) is one of the most frequent routine procedures in urology. Because of the semisterile environment, postoperative...
BACKGROUND
Transurethral resection of the prostate (TUR-P) is one of the most frequent routine procedures in urology. Because of the semisterile environment, postoperative infections, including sepsis, are a common complication, with Escherichia coli, Klebsiella spp., Proteus mirabilis or Enterococcus faecalis as frequently isolated pathogens. Facklamia hominis is a gram-positive, facultatively anaerobic, alpha-hemolytic, catalase-negative coccus that was first described in 1997. To date, only a few cases of infectious complications have been described. We report the first case of postoperative bacteremia due to Facklamia hominis after TUR-P.
CASE PRESENTATION
An 82-year-old man developed fever only a few hours after elective TUR-P because of benign prostate syndrome. After cultivation of blood cultures, antibiotic therapy with ceftriaxone was intravenously administered and changed to oral cotrimoxazole before discharge of the afebrile patient. One anaerobic blood culture revealed Facklamia hominis. Under antibiotic therapy, the patient remained afebrile and showed no signs of infections during follow-up.
CONCLUSIONS
Fever and bacteremia are frequent complications after TUR-P. This study is the first report of Facklamia hominis in a postoperative blood culture after TUR-P. To date, there are only a few reports of patients with infectious complications and isolation of Facklamia hominis in various patient samples. Because Facklamia hominis resembles viridans streptococci on blood agar analysis, this pathogen may often be misidentified. In this case identification of Facklamia hominis was possible with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. It has been postulated that Facklamia hominis might be a facultative pathogen and that its incidence will increase in the future.
Topics: Aerococcaceae; Aged, 80 and over; Bacteremia; Gram-Positive Bacterial Infections; Humans; Male; Postoperative Complications; Transurethral Resection of Prostate
PubMed: 33287796
DOI: 10.1186/s12894-020-00762-8 -
Frontiers in Medicine 2017is a Gram-positive, obligate anaerobic coccus, with a characteristic morphology. Only 22 cases of human infections by this microorganism, including 7 in children, have...
is a Gram-positive, obligate anaerobic coccus, with a characteristic morphology. Only 22 cases of human infections by this microorganism, including 7 in children, have been reported in literature so far. Affected subjects usually present with abdominal pain, nausea, vomiting, and delayed gastric emptying. However, life-threatening complications, like emphysematous gastritis and gastric perforation have also been described. Gastroparesis and gastric outlet obstruction have been considered as a potential etiologic factor. All pediatric cases described thus far presented with concomitant gastrointestinal pathology, such as gastritis, celiac disease, infection with or spp. Here, we report two children with infection, in whom the diagnosis was established by typical histological findings in mucosal biopsies. The first child presented with hematemesis due to ulcerative esophagitis and gastritis, the second child with a history of esophageal stricture had ulcerative gastritis. Confirmation of infection is feasible by molecular microbiota detection methods, since this microorganism cannot be detected by classical culture techniques. Prompt treatment with antibiotics could prevent life-threatening complications.
PubMed: 28913339
DOI: 10.3389/fmed.2017.00145 -
-associated septic arthritis and osteomyelitis in a woman with osteoarthritis and diabetes mellitus.BMJ Case Reports Jun 2017, a Gram-positive obligatory anaerobic coccus, is a commensal of the human vagina and gut and can be an opportunistic pathogen in immunocompromised patients. It is...
, a Gram-positive obligatory anaerobic coccus, is a commensal of the human vagina and gut and can be an opportunistic pathogen in immunocompromised patients. It is usually part of polymicrobial anaerobic infections such as skin and soft tissue infections in diabetics, bone and joint infections and surgical site infections; however, infections caused by in pure culture have been reported as well. Herein, we describe a case of septic arthritis and osteomyelitis caused by in a woman with osteoarthritis and diabetes mellitus.
Topics: Administration, Intravenous; Anti-Bacterial Agents; Arthritis, Infectious; Arthroplasty, Replacement, Hip; Diabetes Complications; Diabetes Mellitus; Diagnosis, Differential; Female; Gram-Positive Bacterial Infections; Humans; Middle Aged; Osteoarthritis; Osteomyelitis; Peptostreptococcus; Treatment Outcome; Vancomycin
PubMed: 28576913
DOI: 10.1136/bcr-2017-219969 -
Journal of Indian Society of... 2019is a Gram-positive, facultative anaerobic coccus that can survive under harsh conditions. Studies have shown a positive relationship between biofilm formation and gelE...
BACKGROUND
is a Gram-positive, facultative anaerobic coccus that can survive under harsh conditions. Studies have shown a positive relationship between biofilm formation and gelE gene expression in . The production of gelatinase (MMP 2) has been detected in 50% of isolates from endodontic and periodontal infections, which suggests its role in the pathogenesis of apical and marginal periodontitis. Although is not considered a periodontopathogen, this species has been more frequently detected in subgingival samples with periodontitis than from periodontally healthy subjects, suggesting that the local conditions in periodontitis may favor its colonization. Hence, the aim of the current study was to detect and compare the presence of in subgingival biofilms of healthy, gingivitis, and periodontitis subjects.
MATERIALS AND METHODS
A total of 100 subjects aged between 25 and 55 years, from the Outpatient Department of Periodontics were recruited for the study. All the subjects were screened for gingival and periodontal status using plaque index, gingival index, and clinical attachment loss. They were divided into three groups based on the clinical findings.• Group A: 18 healthy individuals (gingival index with score zero)• Group B: 34 gingivitis patients (gingival index with score >1)• Group C: 48 chronic periodontitis patients (clinical attachment loss >5 mm in >30% of sites).Subgingival plaque samples of all the enrolled subjects were collected using a sterile curette, later poured into a transport medium (Viability Medium Goteborg Agar III) and sent for microbial culturing within 2 h for detection of .
RESULTS
was detected in 26.8% of all samples evaluated. There was a significantly higher frequency of in subgingival biofilms of periodontitis group (41.7%), compared to gingivitis (5.9%) and healthy group (0%).
CONCLUSION
Enterococci may contribute to increased collagen and periodontal destruction and may further lead to disease progression in patients with chronic periodontitis.
PubMed: 31543613
DOI: 10.4103/jisp.jisp_44_19 -
Infectious Diseases in Obstetrics and... 2019Surgery for gynecologic cancer with lymphadenectomy and pelvic radiotherapy can produce lymphoceles that sometimes complicate with infection, resulting in abscesses. The...
Profiling of the Causative Bacteria in Infected Lymphocysts after Lymphadenectomy for Gynecologic Cancer by Pyrosequencing the 16S Ribosomal RNA Gene Using Next-Generation Sequencing Technology.
BACKGROUND
Surgery for gynecologic cancer with lymphadenectomy and pelvic radiotherapy can produce lymphoceles that sometimes complicate with infection, resulting in abscesses. The true pathogenic bacteria of abscesses are not always found because of false-negative results due to administered antibiotics and difficulty with detection, including for anaerobic bacteria. Analyzing bacteria flora by next-generation sequencing (NGS) using 16S ribosomal DNA may reveal the true pathogenic bacteria in abscesses. This is the first report on causative pathogens for infectious lymphocele using this technology.
METHODS
The subjects were patients who developed infectious lymphocele after surgery for gynecologic cancer at our hospital from July 2015 to September 2016. NGS analyses of bacterial flora were performed using specimens preserved at -80°C. Two steps of PCR were performed for purified DNA samples to obtain sequence libraries. Processing of sequence data, including operational taxonomic unit (OTU) definition, taxonomy assignment, and an OTU BLAST search were performed. All patients gave written informed consent and the study was approved by the institutional research ethics committee.
RESULTS
Six patients underwent puncture and drainage. The result in most cases indicated a single causative pathogen, including and . NGS revealed that the causative bacteria in lymphocele infection are normally a single strain, such as a surface Gram-positive coccus or enteric bacteria. Antibiotics should be chosen as appropriate for elimination of these respective bacteria.
Topics: DNA, Bacterial; DNA, Ribosomal; Female; Genital Neoplasms, Female; High-Throughput Nucleotide Sequencing; Humans; Lymph Node Excision; Lymphocele
PubMed: 30914831
DOI: 10.1155/2019/9326285 -
MicrobiologyOpen Aug 2017The human gut is composed of a large diversity of microorganisms, which have been poorly described. Here, using culturomics, a new concept based on the variation in...
The human gut is composed of a large diversity of microorganisms, which have been poorly described. Here, using culturomics, a new concept based on the variation in culture conditions and MALDI-TOF MS identification, we proceed to explore the microbial diversity of the complex ecosystem of the human gut. Using this approach, we isolated strain AT8 (=CSUR P2118 = DSM 101782) from stool specimens collected from a 51-year-old obese French woman. Strain AT8 is a strictly anaerobic, nonmotile, nonspore-forming gram-positive coccus that do not exhibit catalase and oxidase activities. 16S rDNA-based identification of strain AT8 demonstrated 92% gene sequence similarity with Eggerthella lenta DSM 2243, the phylogenetically closed validly named type species. Here, we present a set of features for the strain AT8 and the description of its complete genome sequence and annotation. The 2,091,845 bp long genome has a G+C content of 63.46% and encodes1,849 predicted genes; 1,781 were protein-coding genes, and 68 were RNAs. On the basis of the characteristics reported here, we propose the creation of a new bacterial genus Hugonella gen. nov., belonging to the Eggerthellaceae family and including Hugonella massiliensis gen. nov., sp. nov., strain AT8 as the type strain.
Topics: Actinobacteria; Anaerobiosis; Base Composition; Cluster Analysis; DNA, Ribosomal; Feces; Female; France; Gastrointestinal Tract; Humans; Middle Aged; Molecular Sequence Annotation; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Whole Genome Sequencing
PubMed: 28326685
DOI: 10.1002/mbo3.458 -
Frontiers in Medicine 2022is an anaerobic Gram-positive coccus frequently found in the oral cavity and gastrointestinal tract, but rarely in the lung. Therefore, pneumonia caused by is also...
is an anaerobic Gram-positive coccus frequently found in the oral cavity and gastrointestinal tract, but rarely in the lung. Therefore, pneumonia caused by is also rare. Although there are some reports of related pneumonia due to aspiration or blood-borne infection with definite remote infection source, there are no reported cases of hematogenous pneumonia in healthy adults lacking a remote source of infection. Herein, we described the intact disease of -related pneumonia mimicking hematogenous pneumonia in terms of chest imagery and diagnosed metagenomic next-generation sequencing (mNGS). Interestingly, there was no clear remote pathogenic source identified in the patient. Microbiome analysis revealed dysbiosis of the oral flora possibly related to poor oral hygiene and a long history of smoking. The patient was treated with moxifloxacin for 3 months. Ultimately, computed tomography (CT) of the chest showed total resolution of the lung lesion. Clinicians need to update the etiology of community-acquired pneumonia. When antibiotic therapy is not effective, pathogen examination becomes very important. New methods of pathogen detection such as mNGS should be employed to this end. For the treatment of pneumonia, no standardized course of treatment was reported. Imaging absorption of lung infections may provide a more objective guidance for the duration of antibiotics in pneumonia.
PubMed: 36388879
DOI: 10.3389/fmed.2022.1017074 -
Anaerobe Oct 2019Clostridium ventriculi (formerly Sarcina ventriculi) is a Gram-positive, obligate anaerobic coccus. Human infections due to this bacterium have rarely been reported, its...
Clostridium ventriculi (formerly Sarcina ventriculi) is a Gram-positive, obligate anaerobic coccus. Human infections due to this bacterium have rarely been reported, its involvement in the development of gastric ulcers and perforation has been suggested. We present a case of bacteremia due to C. ventriculi following acute colonic pseudo-obstruction.
Topics: Aged; Bacteremia; Clostridium; Clostridium Infections; Colonic Pseudo-Obstruction; Humans; Male
PubMed: 31103530
DOI: 10.1016/j.anaerobe.2019.05.005 -
Cureus Jun 2020Background , a facultative aero-anaerobic Gram-positive coccus typically considered normal flora in the human gastrointestinal tract, have increasingly become a major...
Background , a facultative aero-anaerobic Gram-positive coccus typically considered normal flora in the human gastrointestinal tract, have increasingly become a major cause of healthcare-associated infections over the past decade. This study aimed to evaluate the changes in immune factors in pregnant women colonized by in the town of Bafang, West Region of Cameroon. Methods A cross-sectional study was carried in antenatal care unit in various health center in Bafang. were isolated in stools using specific bacterial culture media. Antimicrobial susceptibility test was carried out using disk diffusion method. Blood was used to measure CD3, CD4 and CD8 T-cell lymphocyte counts, white blood cell count, high sensitivity C-reactive protein (hs-CRP) and interleukin-6 measurement using flow cytometry, optical detection and the ELISA solid phase direct sandwich method, respectively. Results Out of the 169 patients studied, 76.30% patients were pregnant women and 23.70% were non-pregnant women. were isolated in 70.41% participants that is, 78.15% in pregnant and 21.85% in non-pregnant women. The mean age was significantly higher in non-pregnant women (29.38 ± 7.685 years) compared to pregnant women (25.55 ± 5.521 years). CD4 T-cell (574.80 ± 165.94; 754.03 ± 162.28, p < 0.001), were significantly lower in pregnant than non-pregnant women respectively, contrary for CD8 T-cell (333.86 ± 233.04; 250.40 ± 227.75, p = 0.043). were significantly more isolated in pregnant women with a CD4 T-cell count between 410 and 625 cells/µl (p < 0.001). were more susceptible to imipenem (91.40%), (100%); ciprofloxacin (65.59%), (69.44%); amikacin (96.77%), (100%) and resistant to chloramphenicol (78.49%), doxycycline (64.52%) and cefotaxime (51.61%) in pregnant women. showed a significant increased multidrug resistant (MDR) and methicillin-resistant (MRSA) phenotypes in pregnant compared to non-pregnant women (p < 0.05). Conclusion The present study revealed that, , including resistant phenotypes should be considered in pregnant women to improve their health care.
PubMed: 32572361
DOI: 10.7759/cureus.8648 -
Case Reports in Infectious Diseases 2021is an oral anaerobic Gram-positive coccus and is a commensal of the mouth, and it is rarely isolated in prosthetic joint infections (PJIs) and even less frequently...
is an oral anaerobic Gram-positive coccus and is a commensal of the mouth, and it is rarely isolated in prosthetic joint infections (PJIs) and even less frequently related to a preceding dental procedure with eventual hematogenous seeding of the prosthetic joint. Here, we present a case of a 56-year-old male with a prosthetic hip joint who developed prosthetic hip joint infection with symptoms starting a few days after a dental procedure and not having received periprocedural antibiotic prophylaxis. He recovered well with surgical intervention and antimicrobial therapy. We conducted a literature review of prosthetic hip joint infections caused by as well as briefly discuss current guidelines on antibiotic prophylaxis in patients with prosthetic joints undergoing dental procedures and some knowledge gaps.
PubMed: 34589242
DOI: 10.1155/2021/9042790