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Acta Stomatologica Croatica Dec 2019The aim this study was to evaluate the factors that influence the presence or absence of the interproximal papilla between implants adjacent to the teeth or other...
AIM
The aim this study was to evaluate the factors that influence the presence or absence of the interproximal papilla between implants adjacent to the teeth or other implants, through clinical and radiographic evaluation.
MATERIAL AND METHODS
The non-probabilistic sample comprised 44 patients of both genders aged between 21 and 68 years, rehabilitated with 114 osseointegrated implants. Through a retrospective clinical study, the patients were divided according to the presence or absence of the interproximal papilla: Group 1 - Absence of Papilla, Group 2 - Partial Presence of Papilla and Group 3 - Total Presence of Papilla. The success of the implants, the periodontal biotype, and the vertical and horizontal distances of the interproximal regions included in the study were evaluated.
RESULTS
Of the 114 implants, 46.5% were considered unsuccessful, and bleeding was present in 29.8%. The periodontal biotype presented as thin and scalloped was found in 85.1% of the regions. The evaluation of the groups according to the confirmation of the interproximal space showed a statistically significant difference (p = 0.007), with 61.9% of the wide and long interproximal spaces classified as Group 1, while 31% of the narrow and short interproximal spaces were classified as Group 3.
CONCLUSION
It was concluded that the morphology of the interproximal space was the factor that was most strongly associated with the presence or absence of the interproximal papilla.
PubMed: 32099259
DOI: 10.15644/asc53/4/4 -
International Journal of Dentistry 2019This study was conducted to identify the morphometric features of the hard palate and to test the reliability of using palatal morphology in sex determination. Three...
This study was conducted to identify the morphometric features of the hard palate and to test the reliability of using palatal morphology in sex determination. Three hundred maxillary casts were collected from dental clinics in north Jordan. The age and gender of the patient and the serial number for each cast were recorded. The age range was 6 to 50 years old. A caliper was used to perform the following measurements: the length, width, and depth of the hard palate. In addition, the size, shape, and position of the incisive papilla were also determined. All measurements were done by a trained examiner who was able to perform the measurements in a reproducible manner. Statistical analysis showed that the mean palatal length, width, and depth, and size of dental papilla in both groups were the highest in males. The full logistic regression model including all the three predictors (length, width, and depth) indicated that the three parameters were significantly correlated with gender in the adult group. However, in the child group, only width and length were significantly (=0.001, > 0.042 respectively) correlated with gender. Regarding the shape and size of the incisive papilla, they were significantly different between males and females in both adult ( > 0.03) and child (=0.001) groups. These findings might be potentially relevant to anthropological studies aiming at individual and/or sex identification. Moreover, the results might have clinical value in prosthodontics, especially in fabricating complete maxillary dentures for edentulous patients.
PubMed: 30809259
DOI: 10.1155/2019/1687345 -
Journal of Oral and Maxillofacial... 2017The human gingiva, characterized by its outstanding scarless wound healing properties, is a unique tissue and a pivotal component of the periodontal apparatus, investing...
The human gingiva, characterized by its outstanding scarless wound healing properties, is a unique tissue and a pivotal component of the periodontal apparatus, investing and surrounding the teeth in their sockets in the alveolar bone. In the last year's gingival mesenchymal stem/progenitor cells (GMSCs), with promising regenerative and immunomodulatory properties, have been isolated and characterized from the gingival lamina propria. These cells, in contrast to other mesenchymal stem/progenitor cell (MSC) sources, are abundant, readily accessible and easily obtainable through minimally invasive cell isolation techniques. This short communication summarizes the current scientific evidence on GMSCs.
PubMed: 28932043
DOI: 10.4103/jomfp.JOMFP_162_17 -
PloS One 2017Teneurins are transmembrane proteins consisting of four paralogues (Ten-1-4), notably expressed in the central nervous system during development. All teneurins contain a...
Teneurins are transmembrane proteins consisting of four paralogues (Ten-1-4), notably expressed in the central nervous system during development. All teneurins contain a bioactive peptide in their carboxyl terminal named teneurin C-terminal associated peptide (TCAP). The present study analyzed the detailed distribution of teneurin-2-like immunoreactive (Ten-2-LI) cells in developing and mature rat molar teeth, as well as in mature human dental pulps. Ten-2 and TCAP-2 genic expressions were also evaluated in rat and human dental pulps. Finally, Ten-2-LI cells were analyzed during the repair process after dentin-pulp complex injury in rat lower molar teeth. For this, histological sections of rat molar teeth and human dental pulps were submitted to immunohistochemical techniques, while total RNA from developing rat teeth and mature human dental pulps were submitted to conventional RT-PCR. Ten-2-LI cells were evident in the initial bell stage of rat molar teeth development, especially in ectomesenchymal cells of the dental papilla. Ten-2-LI odontoblasts showed strong immunoreactivity in rat and human mature teeth. Ten-2 and TCAP-2 genic expressions were confirmed in rat and human dental pulps. Dentin-pulp complex injury resulted in a decrease of Ten-2-LI odontoblasts after traumatic injury. Interestingly, Ten-2-LI cells were also evident in the pulp cell-rich zone in all postoperative days. In conclusion, Ten-2-LI presence in rat and human odontoblasts was demonstrated for the first time and Ten-2/TCAP-2 genic expressions were confirmed in rat and human dental pulps. Furthermore, it was revealed that Ten-2-LI rat odontoblasts can be modulated during the regenerative process.
Topics: Animals; Cells, Cultured; Dental Pulp; Dentin; Female; Humans; Immunohistochemistry; Male; Microscopy, Confocal; Molar; Molar, Third; Nerve Tissue Proteins; Odontoblasts; Rats; Rats, Wistar
PubMed: 28926618
DOI: 10.1371/journal.pone.0184794 -
Clinical and Experimental Dental... Jun 2023To determine the relationship between different parameters (age, periodontal phenotype, contact point height, and crown shape) and the height of the interproximal...
OBJECTIVES
To determine the relationship between different parameters (age, periodontal phenotype, contact point height, and crown shape) and the height of the interproximal papilla around the teeth of/in the maxillary anterior sector.
MATERIAL AND METHODS
A total of 45 subjects were involved in this study, with 315 interproximal papillae of the maxillary anterior sector. The interproximal papillae were clinically classified according to the Norland and Tarnow classification. The periodontal phenotype was assessed by periodontal probe transparency through the marginal gingiva. The height of the papilla, the height of the contact points, and the width/length ratio of the crown were also measured using the periodontal probe. The relationship between the variables was studied using Pearson's correlation. Statistical significance was set at a value of p < .05.
RESULTS
A positive correlation was found between age and the papilla score. However, a negative correlation was noted between age and papilla height, with statistically significant values. A negative correlation was found between the papilla score and the rest of the studied clinical parameters. However, this correlation was not found with regard to the height of the papilla with the same parameters, except for the height of the contact points.
CONCLUSIONS
A statistically significant relationship was noted between the appearance of the interproximal papillae and all the parameters studied.
Topics: Cross-Sectional Studies; Gingiva; Tooth; Tooth Crown; Crowns
PubMed: 36949730
DOI: 10.1002/cre2.728 -
International Journal of Dentistry 2022The aim of this study was to determine the average and maximum height of the papilla around maxillary anterior implants in respect of neighboring structures and location...
OBJECTIVE
The aim of this study was to determine the average and maximum height of the papilla around maxillary anterior implants in respect of neighboring structures and location of implants.
MATERIALS AND METHODS
92 dental implants from 63 patients were investigated in this study. Those implants were placed in the anterior maxillary region and had been loaded for a minimum of one year. After receiving written consent, clinical data including the height of interproximal papillae adjacent to the tooth/implant/pontic were obtained through clinical observation. The independent -test or ANOVA, the regression modeling, and generalized estimating equation (GEE) models were used for statistical analysis ( < 0.05).
RESULTS
Papilla height was calculated as 2.8 mm (1-5.5 mm) for implant-tooth sites, 2.6 mm (1-4 mm) in implants beside pontics, and 2.5 mm (1-3.5 mm) for implants adjacent to implants. Despite the lack of a significant difference in the mean papilla height in the studied groups, the maximum values of papilla heights were significantly different.
CONCLUSIONS
In this study, no significant differences were found in papilla height mean values in relation to neighboring structures or location of implants in the anterior maxilla. However, the maximum values of papilla heights were observed around implants next to natural teeth.
PubMed: 35186085
DOI: 10.1155/2022/4235946 -
F1000Research 2018Primordial odontogenic tumors are a rare recently described mixed odontogenic tumor composed histopathologically of dental papilla like tissue and enamel organ like...
Primordial odontogenic tumors are a rare recently described mixed odontogenic tumor composed histopathologically of dental papilla like tissue and enamel organ like tissue. Only nine cases have been documented worldwide and we are reporting the tenth case which is from Egypt. A 2-year-old Egyptian boy that presented with an asymptomatic swelling of the mandible which appeared with multilocular radiolucency associated with an impacted developing tooth on a computerized tomography (CT) scan. The lesion was excised and diagnosed as a primordial odontogenic tumor. The patient was followed up for two years with no recurrence. Differentiation of primordial odontogenic tumors from other odontogenic tumors, which resemble it histopathologically is crucial to avoid unnecessary aggressive treatment.
PubMed: 29904598
DOI: 10.12688/f1000research.14735.1 -
Journal of Conservative Dentistry : JCD Nov 2014Endodontic and periodontal microsurgery has surpassed the success rates for traditional endodontic and periodontal surgical procedures. Excellent healing results are... (Review)
Review
Endodontic and periodontal microsurgery has surpassed the success rates for traditional endodontic and periodontal surgical procedures. Excellent healing results are being attributed to both the techniques, when employed, for isolated endodontic or periodontal defects. Combined endodontic-periodontal lesions have been referred to as a true challenge, requiring not only endodontic microsurgical management but also concurrent bone grafting and membrane barriers techniques. The prevention of epithelial downgrowth and regeneration of periodontal cementum, fiber, and bone seals the fate of these cases. Achieving primary closure with submergence of grafts has a positive effect on GTR outcome. New techniques of periodontal microsurgery, such as minimally invasive papilla preserving flaps with passive internal mattress suturing, have managed to obtain 90% primary flap closure over grafted sites. Root surface treatment and conditioning has also shown to be beneficial for GTR. Endodontic microsurgery for the combined lesion has not integrated these advances yet. These advances, along with a recently suggested treatment strategy, are ushering in the level next in management of the combined lesions. This article offers an overview of the combined lesion, the disease, its classification, treatment strategy, regenerative tools, microsurgical recommendations, and outcome studies.
PubMed: 25506135
DOI: 10.4103/0972-0707.144571 -
The International Journal of... May 2021SIRT4 is a mitochondrial sirtuin. Owing to its dependance on the cofactor nicotinamide adenine dinucleotide (NAD), SIRT4 can act as a mitochondrial metabolic sensor of...
INTRODUCTION
SIRT4 is a mitochondrial sirtuin. Owing to its dependance on the cofactor nicotinamide adenine dinucleotide (NAD), SIRT4 can act as a mitochondrial metabolic sensor of cellular energy status. We have previously shown that enhancement of mitochondrial functions is vital for the odontogenic diff ;erentiation of dental papilla cells (DPCs) during dentinogenesis. However, whether SIRT4 serves as an effective regulator of DPC diff ;erentiation by affecting mitochondrial functions remains unexplored.
METHODS
Primary DPCs obtained from the first molar dental papilla of neonatal Sprague-Dawley rats were used in this study. The expression pattern of SIRT4 was observed by immunohistochemistry in the first molar of postnatal day 1 (P1) rats. The changes in SIRT4 expression during odontogenic DPC differentiation were evaluated using real-time quantitative polymerase chain reaction (PCR), western blotting, and immunofluorescence. DPCs with loss (small interfering RNA-mediated knockdown) and gain (plasmid transfection-induced overexpression) of SIRT4 function were used to explore the role of SIRT4 in odontogenic differentiation. Mitochondrial function assays were performed using ATP, reactive oxygen species (ROS), and NAD/NADH kits to investigate the potential mechanisms involved in SIRT4-mediated dentinogenesis.
RESULTS
In the present study, we found that SIRT4 expression increased in a time-dependent manner during odontogenic differentiation bothin vivo and in vitro. Sirt4 knockdown resulted in reduced odontogenic differentiation and mineralization, whereas an opposite effect was observed with SIRT4 overexpression. Furthermore, our results verified that in addition to reducing DPC differentiation, Sirt4 knockdown could also significantly reduce ATP levels, elevate the NAD/NADH ratio, and increase ROS levels.
CONCLUSION
SIRT4 regulates mitochondrial functions and the antioxidant capacity of DPCs, thereby influencing dentin formation and tooth development, a phenomenon that may provide a foundation for better understanding the specific molecular mechanisms underlying dentin regeneration.
Topics: Animals; Animals, Newborn; Cell Differentiation; Dental Papilla; Mitochondria; Models, Animal; Odontogenesis; Primary Cell Culture; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; Sirtuins
PubMed: 33636397
DOI: 10.1016/j.biocel.2021.105962 -
World Journal of Stem Cells Jun 2015Stem cells are pluripotent cells, having a property of differentiating into various types of cells of human body. Several studies have developed mesenchymal stem cells... (Review)
Review
Stem cells are pluripotent cells, having a property of differentiating into various types of cells of human body. Several studies have developed mesenchymal stem cells (MSCs) from various human tissues, peripheral blood and body fluids. These cells are then characterized by cellular and molecular markers to understand their specific phenotypes. Dental pulp stem cells (DPSCs) are having a MSCs phenotype and they are differentiated into neuron, cardiomyocytes, chondrocytes, osteoblasts, liver cells and β cells of islet of pancreas. Thus, DPSCs have shown great potentiality to use in regenerative medicine for treatment of various human diseases including dental related problems. These cells can also be developed into induced pluripotent stem cells by incorporation of pluripotency markers and use for regenerative therapies of various diseases. The DPSCs are derived from various dental tissues such as human exfoliated deciduous teeth, apical papilla, periodontal ligament and dental follicle tissue. This review will overview the information about isolation, cellular and molecular characterization and differentiation of DPSCs into various types of human cells and thus these cells have important applications in regenerative therapies for various diseases. This review will be most useful for postgraduate dental students as well as scientists working in the field of oral pathology and oral medicine.
PubMed: 26131314
DOI: 10.4252/wjsc.v7.i5.839