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Tropical Medicine and Infectious Disease May 2020is a Gram-negative, rod-shaped, aerobic bacterium that belongs in the family Pseudomonadaceae and has been isolated from water and soil. Even though it is thought to... (Review)
Review
is a Gram-negative, rod-shaped, aerobic bacterium that belongs in the family Pseudomonadaceae and has been isolated from water and soil. Even though it is thought to cause infections quite rarely in humans, it can cause severe infections even in immunocompetent individuals. The aim of this study was to systemically review all cases of human infection by in the literature and describe their epidemiology, microbiology, antimicrobial susceptibility, treatment and outcomes. Thus, a systematic review of PubMed for studies providing epidemiological, clinical, microbiological as well as treatment data and outcomes of infections was conducted. In total, 12 studies, containing data of 16 patients, were included. The commonest infections were infective endocarditis, central nervous system infections and skin and soft tissue infections (SSTIs). Fever was the main presenting symptom, while sepsis was evident in almost half the patients. was susceptible to most antibiotics tested. Mortality was low in all different infection types. Third or fourth generation cephalosporins and quinolones are the commonest agents used for treatment, irrespectively of the infection site.
PubMed: 32375225
DOI: 10.3390/tropicalmed5020071 -
Genes Jan 2021is an environmental bacterium, rarely isolated in clinical specimens, although it has been described as producing endocarditis and sepsis. Little is known about its...
is an environmental bacterium, rarely isolated in clinical specimens, although it has been described as producing endocarditis and sepsis. Little is known about its genome. Whole genome sequencing can be used to learn about the phylogeny, evolution, or pathogenicity of these isolates. Thus, the aim of this study was to analyze the resistome, virulome, and phylogenetic relationship of two strains, Ps542 and Ps799, isolated from a healthy fecal sample and a lettuce, respectively. Among all of the small number of genomes available in the National Center for Biotechnology Information (NCBI) repository, both strains were placed within one of two well-defined phylogenetic clusters. Both strains lacked antimicrobial resistance genes, but the Ps799 genome showed a MOB family relaxase. Nevertheless, this study revealed that possesses an important number of virulence factors, including a leukotoxin, flagella, pili, and the Type 2 and Type 6 Secretion Systems, that could be responsible for their pathogenesis. More phenotypical and in vivo studies are needed to deepen the association with human infections and the potential pathogenicity.
Topics: Animals; Anti-Bacterial Agents; Bacterial Proteins; Drug Resistance, Microbial; Ducks; Endocarditis; Endodeoxyribonucleases; Feces; Food Microbiology; Genome, Bacterial; Humans; Lactuca; Phylogeny; Pseudomonas mendocina; Sepsis; Virulence Factors; Whole Genome Sequencing
PubMed: 33477842
DOI: 10.3390/genes12010115 -
Cureus Mar 2022is a Gram-negative bacillus from the family Pseudomonadaceae. The first -related infection was reported in 1992. Although a rare cause of infections, has been known...
is a Gram-negative bacillus from the family Pseudomonadaceae. The first -related infection was reported in 1992. Although a rare cause of infections, has been known to cause severe infections that require intensive treatment. We present the first documented case of urinary tract infection caused by An 83-year-old male with a past medical history of diabetes, hypertension, coronary artery disease, and prostate cancer with bone metastases, currently being treated with abiraterone and prednisone, presented with subjective fever, fatigue, altered mental status, dysuria, and hematuria of one-week duration. He was found to have a complicated urinary tract infection with an incidental asymptomatic COVID-19 infection on admission. The patient was empirically treated with ceftriaxone and switched to cefepime for broader coverage on day two of hospitalization. Urine culture reported the presence of with resistance only to fluoroquinolones. Ceftriaxone was reinstated. The patient was successfully treated with a seven-day course of ceftriaxone (days 1-3, days 6-7) and cefepime (days 4-5) but continued to remain inpatient for a later symptomatic COVID-19 pneumonia with discharge on day 15. The majority of . infections present as skin and soft tissue infections, infective endocarditis, meningitis, and bacteremia. Ours is the first documented case of urinary tract infection caused by particularly in an immunocompromised COVID-19 patient, and the second to report with resistance to fluoroquinolones. This report contributes to the growing literature regarding -related infections.
PubMed: 35495004
DOI: 10.7759/cureus.23583 -
The American Journal of Case Reports Apr 2019BACKGROUND Pseudomonas mendocina is a Gram-negative, aerobic, rod-shaped bacterium belonging to the family Pseudomonadaceae. In nature, P. mendocina has been isolated... (Review)
Review
BACKGROUND Pseudomonas mendocina is a Gram-negative, aerobic, rod-shaped bacterium belonging to the family Pseudomonadaceae. In nature, P. mendocina has been isolated from water and soil samples. The species rarely causes disease in humans though severe infections resulting in hospitalization and intensive care have been documented. This case is perhaps the second reported case in the United States of a P. mendocina related infection. In this case report, we analyze the clinical and laboratory features of P. mendocina infection in a severely immunocompromised acquired immunodeficiency syndrome (AIDS) patient and review the available literature. CASE REPORT A 64-year-old white male with past medical history significant for human immunodeficiency virus (HIV)/AIDS (CD⁴ count on admission <10 cells/mm³) diagnosed in 1988 and on antiretroviral therapy since 1992, was admitted to our facility for acute management of a suspected invasive mold infection. On hospital day 20 the patient developed a fever of 39.9°C, had an elevated lactate of 2.6 mmol/L and absolute neutrophil count greater than 1000 cells/mm³. On hospital day 22, both blood culture sets were positive for Pseudomonas mendocina. Antibiotic therapy was de-escalated to ceftazidime and after a total treatment course of 10 days the was successfully discharged. CONCLUSIONS There have been 14 reported cases of P. mendocina in the world. Four cases presented with meningitis and 5 with endocarditis. Beyond typical anti-pseudomonal agents, 2 of the reported cases show susceptibility of P. mendocina antibiotics such as sulfamethoxazole/trimethoprim and ceftriaxone. All documented case reports of P. mendocina infection resulted in successful treatment with antibiotics and survival of the patient.
Topics: Anti-Bacterial Agents; Bacteremia; Ceftazidime; HIV Infections; Humans; Immunocompromised Host; Male; Middle Aged; Pseudomonas Infections; Pseudomonas mendocina
PubMed: 30948701
DOI: 10.12659/AJCR.914360 -
3 Biotech Nov 2019was identified as a novel endophytic isolate of with squalene cyclase activity. The PCR amplification of squalene hopene cyclase () gene from the isolate with the...
was identified as a novel endophytic isolate of with squalene cyclase activity. The PCR amplification of squalene hopene cyclase () gene from the isolate with the primers PA1/PA2 showed a band at 1980 bp specific for the enzyme squalene hopene cyclase. The in silico translation of the squalene hopene cyclase gene showed 96% sequence similarity with squalene hopene cyclase of (WP-060782422). Docking studies of the template and the modeled protein with the ligand squalene showed that the main interacting residues were Asp376 and Asp377. Squalene hopene cyclase template 1 sqc.1A sequence from was used as the template for docking experiments. The gene coding for squalene hopene cyclase from has been cloned in pET-28a vector to produce recombinant vector and was expressed in BL21 (DE3) expression system. Squalene hopene cyclase enzyme was isolated, purified and the molecular weight was confirmed by SDS-PAGE as 75 KDa.
PubMed: 31588405
DOI: 10.1007/s13205-019-1901-7 -
3 Biotech Sep 2019This study aimed to investigate the effects of cytoskeleton protein MreB on bacterial cell morphology and the synthesis of alginate oligosaccharides (AO) and...
This study aimed to investigate the effects of cytoskeleton protein MreB on bacterial cell morphology and the synthesis of alginate oligosaccharides (AO) and polyhydroxyalkanoate (PHA) by NK-01. To overexpress the gene, an expression vector encoding MreB-GFP fusion protein was constructed. The scanning electron microscope (SEM) showed that cells expressing MreB were longer than the wild ones, which agrees with MreB's relationship with the synthesis of peptidoglycan. Cells expressing the MreB-GFP fusion protein emitted green fluorescence under a fluorescence microscope, suggesting that MreB was functionally expressed in strain NK-01. Under a confocal laser scanning microscope, MreB was observed as located around the cell membrane. Furthermore, the recombinant strain could synthesize 0.961 g/L AO, which was 5.86-fold higher than wild-type strain. Through the medium optimization test, we finally selected the addition of 20 g/L glucose as the optimal glycogen addition for AO fermentation based on a high AO yield and high substrate transformation efficiency. The results indicated that overexpression of MreB affected the cell morphology, the activity of AO polymerase, and the efficiency of AO secretion. However, the synthesis of PHA for recombinant strain was slightly reduced. The results suggested that the overexpression of this cytoskeleton protein affected the yield of specific intracellular and extracellular products.
PubMed: 31497462
DOI: 10.1007/s13205-019-1873-7 -
Bioengineered Sep 2016The straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of N,P,K and other effective elements...
The straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of N,P,K and other effective elements in ecological system. We isolated a strain through screening the straw degradation strains from natural humic straw in the low temperature area in northeast of china, which can produce cellulase efficiently. The strain was identified as Pseudomonas mendocina by using morphological, physiological, biochemical test, and molecular biological test, with the functional clarification on producing cellulase for Pseudomonas mendocina for the first time. The enzyme force constant Km and the maximum reaction rate (Vmax) of the strain were 0.3261 g/L and 0.1525 mg/(min.L) through the enzyme activity detection, and the molecular weight of the enzyme produced by the strain were 42.4 k and 20.4 k based on SDS-PAGE. The effects of various ecological factors such as temperature, pH and nematodes on the enzyme produced by the strain in the micro ecosystem in plant roots were evaluated. The result showed that the optimum temperature was 28°C, and the best pH was 7.4∼7.8, the impact heavy metal was Pb and the enzyme activity and biomass of Pseudomonas mendocina increased the movement and predation of nematodes.
Topics: Biomass; Cellulase; Enzyme Stability; Hydrogen-Ion Concentration; Metals, Heavy; Molecular Weight; Pseudomonas mendocina; Substrate Specificity; Temperature
PubMed: 27710430
DOI: 10.1080/21655979.2016.1227143 -
MicroPublication Biology 2022experiences and microbiome have been shown to shape its responses to certain stimuli; a recent study found that grown on JUb39 exhibited increased attraction to that...
experiences and microbiome have been shown to shape its responses to certain stimuli; a recent study found that grown on JUb39 exhibited increased attraction to that same growth bacteria while also lowered repulsion to the odor 1-octanol (O’Donnell et al. 2020). This prompted us to ask whether other strains of bacteria could likewise alter responses to bacterial food and volatile chemicals. So, to expand upon current knowledge, we cultured wild-type (N2) on an unidentified ( sp?), (MSPm1), (MYb11), (JUb19), or strain ( sp?). After several generations, we examined how their choice of bacterial food was affected. In addition, we looked at their response to the olfactory stimuli 2-butanone; 2,3-butanedione; 2,3-pentanedione; and 2-nonanone, as well as their response to the gustatory stimulus sodium chloride. Interestingly, we found that growth on any of these bacterial strains led to their bacterial preferences and behavioral responses to 2-butanone; 2,3-pentanedione; diacetyl; and sodium chloride remaining unchanged. However, we also saw that showed a preference for MSPm1 and sp? to HB101, and HB101 to MYb11. Furthermore, worms that are grown on MSPm1 showed stronger attraction to a 1:10 dilution of 2-nonanone (AWB-sensed odorant) as compared to worms grown on the other bacterial strains.
PubMed: 35622520
DOI: 10.17912/micropub.biology.000535 -
3 Biotech Jul 2017Endosulfan contamination is one of the major concerns of soil ecosystem, which causes detrimental effects not only to humans but also to animals and plants. Therefore,...
Endosulfan contamination is one of the major concerns of soil ecosystem, which causes detrimental effects not only to humans but also to animals and plants. Therefore, the aim of this study was to isolate and identify a novel bacterial strain capable of degrading endosulfan in agriculture contaminated soils. A novel bacterial strain was isolated from the sugarcane field contaminated with endosulfan, and was named as ZAM1 strain. The ZAM1 bacterial strain was further identified as Pseudomonas mendocina based on the biochemical and molecular analysis. 16sRNA sequence analysis of ZAM1 strain shows maximum similarity with known endosulfan-degrading bacteria (Pseudomonas putida), respectively. Enrichment was carried out using the endosulfan as sole sulfur source. The ZAM1 strain was able to use α and β endosulfan as a sole sulfur source. Our results showed that ZAM1 strain degrades endosulfan >64.5% (50 mg/l) after 12 days of incubation. The residues were analyzed by GC-MS analysis and confirmed the formation of metabolites of dieldrin, 2 heptanone, methyl propionate, and endosulfan lactone compounds. Hence, these results indicate that the ZAM1 strain is a promising bacterial source for detoxification of endosulfan residues in the environment.
PubMed: 28667651
DOI: 10.1007/s13205-017-0823-5 -
Scientific Reports Feb 2019Polyhydroxyalkanoate (PHA) can be produced by microorganisms from renewable resources and is regarded as a promising bioplastic to replace petroleum-based plastics....
Polyhydroxyalkanoate (PHA) can be produced by microorganisms from renewable resources and is regarded as a promising bioplastic to replace petroleum-based plastics. Pseudomonas mendocina NK-01 is a medium-chain-length PHA (mcl-PHA)-producing strain and its whole-genome sequence is currently available. The yield of mcl-PHA in P. mendocina NK-01 is expected to be improved by applying a promoter engineering strategy. However, a limited number of well-characterized promoters has greatly restricted the application of promoter engineering for increasing the yield of mcl-PHA in P. mendocina NK-01. In this work, 10 endogenous promoters from P. mendocina NK-01 were identified based on RNA-seq and promoter prediction results. Subsequently, 10 putative promoters were characterized for their strength through the expression of a reporter gene gfp. As a result, five strong promoters designated as P4, P6, P9, P16 and P25 were identified based on transcriptional level and GFP fluorescence intensity measurements. To evaluate whether the screened promoters can be used to enhance transcription of PHA synthase gene (phaC), the three promoters P4, P6 and P16 were separately integrated into upstream of the phaC operon in the genome of P. mendocina NK-01, resulting in the recombinant strains NKU-4C1, NKU-6C1 and NKU-16C1. As expected, the transcriptional levels of phaC1 and phaC2 in the recombinant strains were increased as shown by real-time quantitative RT-PCR. The phaZ gene encoding PHA depolymerase was further deleted to construct the recombinant strains NKU-∆phaZ-4C1, NKU-∆phaZ-6C1 and NKU-∆phaZ-16C1. The results from shake-flask fermentation indicated that the mcl-PHA titer of recombinant strain NKU-∆phaZ-16C1 was increased from 17 to 23 wt% compared with strain NKU-∆phaZ. This work provides a feasible method to discover strong promoters in P. mendocina NK-01 and highlights the potential of the screened endogenous strong promoters for metabolic engineering of P. mendocina NK-01 to increase the yield of mcl-PHA.
Topics: Acyltransferases; Bacterial Proteins; Metabolic Engineering; Polyhydroxyalkanoates; Promoter Regions, Genetic; Pseudomonas mendocina; Real-Time Polymerase Chain Reaction
PubMed: 30755729
DOI: 10.1038/s41598-019-39321-z