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Genes Jan 2021is an environmental bacterium, rarely isolated in clinical specimens, although it has been described as producing endocarditis and sepsis. Little is known about its...
is an environmental bacterium, rarely isolated in clinical specimens, although it has been described as producing endocarditis and sepsis. Little is known about its genome. Whole genome sequencing can be used to learn about the phylogeny, evolution, or pathogenicity of these isolates. Thus, the aim of this study was to analyze the resistome, virulome, and phylogenetic relationship of two strains, Ps542 and Ps799, isolated from a healthy fecal sample and a lettuce, respectively. Among all of the small number of genomes available in the National Center for Biotechnology Information (NCBI) repository, both strains were placed within one of two well-defined phylogenetic clusters. Both strains lacked antimicrobial resistance genes, but the Ps799 genome showed a MOB family relaxase. Nevertheless, this study revealed that possesses an important number of virulence factors, including a leukotoxin, flagella, pili, and the Type 2 and Type 6 Secretion Systems, that could be responsible for their pathogenesis. More phenotypical and in vivo studies are needed to deepen the association with human infections and the potential pathogenicity.
Topics: Animals; Anti-Bacterial Agents; Bacterial Proteins; Drug Resistance, Microbial; Ducks; Endocarditis; Endodeoxyribonucleases; Feces; Food Microbiology; Genome, Bacterial; Humans; Lactuca; Phylogeny; Pseudomonas mendocina; Sepsis; Virulence Factors; Whole Genome Sequencing
PubMed: 33477842
DOI: 10.3390/genes12010115 -
Cureus Mar 2022is a Gram-negative bacillus from the family Pseudomonadaceae. The first -related infection was reported in 1992. Although a rare cause of infections, has been known...
is a Gram-negative bacillus from the family Pseudomonadaceae. The first -related infection was reported in 1992. Although a rare cause of infections, has been known to cause severe infections that require intensive treatment. We present the first documented case of urinary tract infection caused by An 83-year-old male with a past medical history of diabetes, hypertension, coronary artery disease, and prostate cancer with bone metastases, currently being treated with abiraterone and prednisone, presented with subjective fever, fatigue, altered mental status, dysuria, and hematuria of one-week duration. He was found to have a complicated urinary tract infection with an incidental asymptomatic COVID-19 infection on admission. The patient was empirically treated with ceftriaxone and switched to cefepime for broader coverage on day two of hospitalization. Urine culture reported the presence of with resistance only to fluoroquinolones. Ceftriaxone was reinstated. The patient was successfully treated with a seven-day course of ceftriaxone (days 1-3, days 6-7) and cefepime (days 4-5) but continued to remain inpatient for a later symptomatic COVID-19 pneumonia with discharge on day 15. The majority of . infections present as skin and soft tissue infections, infective endocarditis, meningitis, and bacteremia. Ours is the first documented case of urinary tract infection caused by particularly in an immunocompromised COVID-19 patient, and the second to report with resistance to fluoroquinolones. This report contributes to the growing literature regarding -related infections.
PubMed: 35495004
DOI: 10.7759/cureus.23583 -
Tropical Medicine and Infectious Disease May 2020is a Gram-negative, rod-shaped, aerobic bacterium that belongs in the family Pseudomonadaceae and has been isolated from water and soil. Even though it is thought to... (Review)
Review
is a Gram-negative, rod-shaped, aerobic bacterium that belongs in the family Pseudomonadaceae and has been isolated from water and soil. Even though it is thought to cause infections quite rarely in humans, it can cause severe infections even in immunocompetent individuals. The aim of this study was to systemically review all cases of human infection by in the literature and describe their epidemiology, microbiology, antimicrobial susceptibility, treatment and outcomes. Thus, a systematic review of PubMed for studies providing epidemiological, clinical, microbiological as well as treatment data and outcomes of infections was conducted. In total, 12 studies, containing data of 16 patients, were included. The commonest infections were infective endocarditis, central nervous system infections and skin and soft tissue infections (SSTIs). Fever was the main presenting symptom, while sepsis was evident in almost half the patients. was susceptible to most antibiotics tested. Mortality was low in all different infection types. Third or fourth generation cephalosporins and quinolones are the commonest agents used for treatment, irrespectively of the infection site.
PubMed: 32375225
DOI: 10.3390/tropicalmed5020071 -
The American Journal of Case Reports Apr 2019BACKGROUND Pseudomonas mendocina is a Gram-negative, aerobic, rod-shaped bacterium belonging to the family Pseudomonadaceae. In nature, P. mendocina has been isolated... (Review)
Review
BACKGROUND Pseudomonas mendocina is a Gram-negative, aerobic, rod-shaped bacterium belonging to the family Pseudomonadaceae. In nature, P. mendocina has been isolated from water and soil samples. The species rarely causes disease in humans though severe infections resulting in hospitalization and intensive care have been documented. This case is perhaps the second reported case in the United States of a P. mendocina related infection. In this case report, we analyze the clinical and laboratory features of P. mendocina infection in a severely immunocompromised acquired immunodeficiency syndrome (AIDS) patient and review the available literature. CASE REPORT A 64-year-old white male with past medical history significant for human immunodeficiency virus (HIV)/AIDS (CD⁴ count on admission <10 cells/mm³) diagnosed in 1988 and on antiretroviral therapy since 1992, was admitted to our facility for acute management of a suspected invasive mold infection. On hospital day 20 the patient developed a fever of 39.9°C, had an elevated lactate of 2.6 mmol/L and absolute neutrophil count greater than 1000 cells/mm³. On hospital day 22, both blood culture sets were positive for Pseudomonas mendocina. Antibiotic therapy was de-escalated to ceftazidime and after a total treatment course of 10 days the was successfully discharged. CONCLUSIONS There have been 14 reported cases of P. mendocina in the world. Four cases presented with meningitis and 5 with endocarditis. Beyond typical anti-pseudomonal agents, 2 of the reported cases show susceptibility of P. mendocina antibiotics such as sulfamethoxazole/trimethoprim and ceftriaxone. All documented case reports of P. mendocina infection resulted in successful treatment with antibiotics and survival of the patient.
Topics: Anti-Bacterial Agents; Bacteremia; Ceftazidime; HIV Infections; Humans; Immunocompromised Host; Male; Middle Aged; Pseudomonas Infections; Pseudomonas mendocina
PubMed: 30948701
DOI: 10.12659/AJCR.914360 -
MicroPublication Biology 2022experiences and microbiome have been shown to shape its responses to certain stimuli; a recent study found that grown on JUb39 exhibited increased attraction to that...
experiences and microbiome have been shown to shape its responses to certain stimuli; a recent study found that grown on JUb39 exhibited increased attraction to that same growth bacteria while also lowered repulsion to the odor 1-octanol (O’Donnell et al. 2020). This prompted us to ask whether other strains of bacteria could likewise alter responses to bacterial food and volatile chemicals. So, to expand upon current knowledge, we cultured wild-type (N2) on an unidentified ( sp?), (MSPm1), (MYb11), (JUb19), or strain ( sp?). After several generations, we examined how their choice of bacterial food was affected. In addition, we looked at their response to the olfactory stimuli 2-butanone; 2,3-butanedione; 2,3-pentanedione; and 2-nonanone, as well as their response to the gustatory stimulus sodium chloride. Interestingly, we found that growth on any of these bacterial strains led to their bacterial preferences and behavioral responses to 2-butanone; 2,3-pentanedione; diacetyl; and sodium chloride remaining unchanged. However, we also saw that showed a preference for MSPm1 and sp? to HB101, and HB101 to MYb11. Furthermore, worms that are grown on MSPm1 showed stronger attraction to a 1:10 dilution of 2-nonanone (AWB-sensed odorant) as compared to worms grown on the other bacterial strains.
PubMed: 35622520
DOI: 10.17912/micropub.biology.000535 -
3 Biotech Nov 2019was identified as a novel endophytic isolate of with squalene cyclase activity. The PCR amplification of squalene hopene cyclase () gene from the isolate with the...
was identified as a novel endophytic isolate of with squalene cyclase activity. The PCR amplification of squalene hopene cyclase () gene from the isolate with the primers PA1/PA2 showed a band at 1980 bp specific for the enzyme squalene hopene cyclase. The in silico translation of the squalene hopene cyclase gene showed 96% sequence similarity with squalene hopene cyclase of (WP-060782422). Docking studies of the template and the modeled protein with the ligand squalene showed that the main interacting residues were Asp376 and Asp377. Squalene hopene cyclase template 1 sqc.1A sequence from was used as the template for docking experiments. The gene coding for squalene hopene cyclase from has been cloned in pET-28a vector to produce recombinant vector and was expressed in BL21 (DE3) expression system. Squalene hopene cyclase enzyme was isolated, purified and the molecular weight was confirmed by SDS-PAGE as 75 KDa.
PubMed: 31588405
DOI: 10.1007/s13205-019-1901-7 -
Bioengineered Sep 2016The straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of N,P,K and other effective elements...
The straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of N,P,K and other effective elements in ecological system. We isolated a strain through screening the straw degradation strains from natural humic straw in the low temperature area in northeast of china, which can produce cellulase efficiently. The strain was identified as Pseudomonas mendocina by using morphological, physiological, biochemical test, and molecular biological test, with the functional clarification on producing cellulase for Pseudomonas mendocina for the first time. The enzyme force constant Km and the maximum reaction rate (Vmax) of the strain were 0.3261 g/L and 0.1525 mg/(min.L) through the enzyme activity detection, and the molecular weight of the enzyme produced by the strain were 42.4 k and 20.4 k based on SDS-PAGE. The effects of various ecological factors such as temperature, pH and nematodes on the enzyme produced by the strain in the micro ecosystem in plant roots were evaluated. The result showed that the optimum temperature was 28°C, and the best pH was 7.4∼7.8, the impact heavy metal was Pb and the enzyme activity and biomass of Pseudomonas mendocina increased the movement and predation of nematodes.
Topics: Biomass; Cellulase; Enzyme Stability; Hydrogen-Ion Concentration; Metals, Heavy; Molecular Weight; Pseudomonas mendocina; Substrate Specificity; Temperature
PubMed: 27710430
DOI: 10.1080/21655979.2016.1227143 -
3 Biotech Jul 2017Endosulfan contamination is one of the major concerns of soil ecosystem, which causes detrimental effects not only to humans but also to animals and plants. Therefore,...
Endosulfan contamination is one of the major concerns of soil ecosystem, which causes detrimental effects not only to humans but also to animals and plants. Therefore, the aim of this study was to isolate and identify a novel bacterial strain capable of degrading endosulfan in agriculture contaminated soils. A novel bacterial strain was isolated from the sugarcane field contaminated with endosulfan, and was named as ZAM1 strain. The ZAM1 bacterial strain was further identified as Pseudomonas mendocina based on the biochemical and molecular analysis. 16sRNA sequence analysis of ZAM1 strain shows maximum similarity with known endosulfan-degrading bacteria (Pseudomonas putida), respectively. Enrichment was carried out using the endosulfan as sole sulfur source. The ZAM1 strain was able to use α and β endosulfan as a sole sulfur source. Our results showed that ZAM1 strain degrades endosulfan >64.5% (50 mg/l) after 12 days of incubation. The residues were analyzed by GC-MS analysis and confirmed the formation of metabolites of dieldrin, 2 heptanone, methyl propionate, and endosulfan lactone compounds. Hence, these results indicate that the ZAM1 strain is a promising bacterial source for detoxification of endosulfan residues in the environment.
PubMed: 28667651
DOI: 10.1007/s13205-017-0823-5 -
Frontiers in Bioengineering and... 2022Metformin is used globally to treat type II diabetes, has demonstrated anti-ageing and COVID mitigation effects and is a major anthropogenic pollutant to be...
Metformin is used globally to treat type II diabetes, has demonstrated anti-ageing and COVID mitigation effects and is a major anthropogenic pollutant to be bioremediated by wastewater treatment plants (WWTPs). Metformin is not adsorbed well by activated carbon and toxic N-chloro derivatives can form in chlorinated water. Most earlier studies on metformin biodegradation have used wastewater consortia and details of the genomes, relevant genes, metabolic products, and potential for horizontal gene transfer are lacking. Here, two metformin-biodegrading bacteria from a WWTP were isolated and their biodegradation characterized. sp. MET metabolized metformin stoichiometrically to guanylurea, an intermediate known to accumulate in some environments including WWTPs. MET completely metabolized metformin and utilized all the nitrogen atoms for growth. MET also metabolized metformin breakdown products sometimes observed in WWTPs: 1-N-methylbiguanide, biguanide, guanylurea, and guanidine. The genome of each bacterium was obtained. Genes involved in the transport of guanylurea in sp. MET were expressed heterologously and shown to serve as an antiporter to expel the toxic guanidinium compound. A novel guanylurea hydrolase enzyme was identified in MET, purified, and characterized. The and each contained one plasmid of 160 kb and 90 kb, respectively. In total, these studies are significant for the bioremediation of a major pollutant in WWTPs today.
PubMed: 36588930
DOI: 10.3389/fbioe.2022.1086261 -
AMB Express Dec 2017Root exudates are chemical compounds that are released from living plant roots and provide significant energy, carbon, nitrogen and phosphorus sources for microbes...
Root exudates are chemical compounds that are released from living plant roots and provide significant energy, carbon, nitrogen and phosphorus sources for microbes inhabiting the rhizosphere. The exudates shape the microflora associated with the plant, as well as influences the plant health and productivity. Therefore, a better understanding of the trophic link that is established between the plant and the associated bacteria is necessary. In this study, a comprehensive survey on the utilization of grapevine and rootstock related organic acids were conducted on a vineyard soil isolate which is Pseudomonas mendocina strain S5.2. Phenotype microarray analysis has demonstrated that this strain can utilize several organic acids including lactic acid, succinic acid, malic acid, citric acid and fumaric acid as sole growth substrates. Complete genome analysis using single molecule real-time technology revealed that the genome consists of a 5,120,146 bp circular chromosome and a 252,328 bp megaplasmid. A series of genetic determinants associated with the carbon utilization signature of the strain were subsequently identified in the chromosome. Of note, the coexistence of genes encoding several iron-sulfur cluster independent isoenzymes in the genome indicated the importance of these enzymes in the events of iron deficiency. Synteny and comparative analysis have also unraveled the unique features of D-lactate dehydrogenase of strain S5.2 in the study. Collective information of this work has provided insights on the metabolic role of this strain in vineyard soil rhizosphere.
PubMed: 28655216
DOI: 10.1186/s13568-017-0437-7