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Journal of Endodontics Nov 2023Neutrophil extracellular traps (NETs) have been described as structures composed of DNA and proteins, such as elastase and myeloperoxidase, that are able to kill...
INTRODUCTION
Neutrophil extracellular traps (NETs) have been described as structures composed of DNA and proteins, such as elastase and myeloperoxidase, that are able to kill bacteria extracellularly. The aim of the present study was to evaluate the role of NETs in bone resorption observed in pulp infection-induced apical periodontitis in mice.
METHODS
Apical periodontitis was experimentally induced by exposing the dental pulp of the mandibular first molar of mice to the oral microenvironment. The expression of NETs was evaluated by immunofluorescence in mice and biopsies of apical periodontitis. Mice were treated with vehicle or DNase I to degrade NETs, and the samples were collected after 7 days. The size of the apical lesion and the osteoclast number were determined in hematoxylin-eosin- and tartrate-resistant acid phosphatase-stained sections, respectively. Osteoclast differentiation and function markers were evaluated by quantitative polymerase chain reaction. The level of NETs in the serum was determined by the myeloperoxidase-DNA PicoGreen assay.
RESULTS
We first confirmed the presence of neutrophils and NETs at the site of the lesion in mice and in biopsies of patients with apical periodontitis. The treatment of mice with DNase I reduced the level of NETs in the serum and led to a reduction in apical lesion size and alveolar bone resorption. This effect was associated with a reduction of local inflammatory infiltrate and a reduced number of osteoclasts. We found that the increased expression of Acp5, Ctsk, and Rankl genes associated with osteoclast formation and function were abrogated by the absence of NETs.
CONCLUSIONS
Our data highlight NETs as an important player in the pathogenesis of apical periodontitis with regard to the local inflammation and consequent bone resorption after pulp infection.
Topics: Periapical Periodontitis; Extracellular Traps; Animals; Osteoclasts; Mice; Neutrophils; Humans; Tartrate-Resistant Acid Phosphatase; Cathepsin K; Male; Alveolar Bone Loss; Deoxyribonuclease I; RANK Ligand
PubMed: 37619708
DOI: 10.1016/j.joen.2023.07.027 -
Journal of the College of Physicians... Dec 2023To investigate the relationship between systemic immune inflammation index (SII), C-reactive protein/albumin ratio (CAR), lymphocyte/CRP ratio (LCR), and apical thrombus...
OBJECTIVE
To investigate the relationship between systemic immune inflammation index (SII), C-reactive protein/albumin ratio (CAR), lymphocyte/CRP ratio (LCR), and apical thrombus development.
STUDY DESIGN
Observational, cross-sectional study. Place and Duration of the Study: Bursa City Hospital, Turkey, from 1st January to 31st March 2023.
METHODOLOGY
Twenty-two patients with a clinical presentation of acute anterior myocardial infarction and diagnosed with LVAT during follow-up were included. Sixty-eight patients with acute anterior myocardial infarction (AMI) were selected as the control group (comparison). Clinical and demographic characteristics, laboratory data, echocardiographic findings, coronary angiography, and percutaneous coronary intervention data were recorded. SII was calculated by the formula Neutrophil (N) × Platelet (P) / Lymphocyte (L). CAR and LCR values were also determined.
RESULTS
While there was no significant difference between the two groups in terms of SII, CAR was significantly higher and LCR was significantly lower in the apical thrombus group. The cut-off value for CAR was 0.165 (sensitivity=63.64%, specificity=74.60%, AUC=0.718; p <0.05). For LCR, the AUC value of 0.382 and below was found to indicate the presence of apical thrombus with a probability of 69.8% (sensitivity=68.18%, specificity=67.16%, p= 0.002).
CONCLUSION
No significant relationship was found between SII and apical thrombus in the detection of LVAT, whereas high CAR and low LCR were associated with the presence of apical thrombus.
KEY WORDS
Left ventricular apical thrombus, Apical aneurysm, C-reactive protein/albumin ratio, Lymphocyte/C-reactive protein ratio, Systemic immune inflammation index.
Topics: Humans; C-Reactive Protein; Cross-Sectional Studies; Thrombosis; Albumins; Myocardial Infarction; Inflammation; Retrospective Studies
PubMed: 38062587
DOI: 10.29271/jcpsp.2023.12.1349 -
The Journal of Cell Biology Sep 2023Apical constriction is a cell shape change that drives key morphogenetic events during development, including gastrulation and neural tube formation. The forces driving...
Apical constriction is a cell shape change that drives key morphogenetic events during development, including gastrulation and neural tube formation. The forces driving apical constriction are primarily generated through the contraction of apicolateral and/or medioapical actomyosin networks. In the Drosophila ventral furrow, the medioapical actomyosin network has a sarcomere-like architecture, with radially polarized actin filaments and centrally enriched non-muscle myosin II and myosin activating kinase. To determine if this is a broadly conserved actin architecture driving apical constriction, we examined actomyosin architecture during C. elegans gastrulation, in which two endodermal precursor cells internalize from the surface of the embryo. Quantification of protein localization showed that neither the non-muscle myosin II NMY-2 nor the myosin-activating kinase MRCK-1 is enriched at the center of the apex. Further, visualization of barbed- and pointed-end capping proteins revealed that actin filaments do not exhibit radial polarization at the apex. Our results demonstrate that C. elegans endodermal precursor cells apically constrict using a mixed-polarity actin filament network and with myosin and a myosin activator distributed throughout the network. Taken together with observations made in other organisms, our results demonstrate that diverse actomyosin architectures are used in animal cells to accomplish apical constriction.
Topics: Animals; Actin Cytoskeleton; Actomyosin; Caenorhabditis elegans; Constriction; Morphogenesis; Myosin Type II; Myosins; Caenorhabditis elegans Proteins
PubMed: 37351566
DOI: 10.1083/jcb.202302102 -
Journal of Plant Physiology Oct 2023Reactive oxygen species (ROS) are initially considered to be toxic byproducts of aerobic metabolic reactions. However, increasing evidence has shown that they have... (Review)
Review
Reactive oxygen species (ROS) are initially considered to be toxic byproducts of aerobic metabolic reactions. However, increasing evidence has shown that they have emerged as signaling molecules involved in several basic biological processes. Recent studies highlight the pivotal role of ROS in the maintenance of shoot and root stem cell niche. In this review, we discuss the impact of ROS distribution and their gradients on the stability of the stem cell niches (SCN) in shoot apical meristem (SAM) and root apical meristem (RAM) by determining the balance between stemness and differentiation. We also summarize several important transcription factors that are involved in the regulation of ROS balance in SAM and RAM, regulating key enzymes in ROS metabolism, especially SOD and peroxidase. ROS are also tightly interconnected with phytohormones in the control of the stem cell fate. Besides, ROS are also important regulators of the cell cycle in controlling the size of the stem cells. Understanding the regulation mechanisms of ROS production, polarization gradient distribution, homeostasis, and downstream signal transduction in cells will open exciting new perspectives for plant developmental biology.
PubMed: 37690340
DOI: 10.1016/j.jplph.2023.154082 -
Compendium of Continuing Education in... 2023The treatment of a peri-implant soft-tissue dehiscence (PSTD) can be quite challenging for many clinicians and leave gravely disappointing esthetic remarks for patients...
The treatment of a peri-implant soft-tissue dehiscence (PSTD) can be quite challenging for many clinicians and leave gravely disappointing esthetic remarks for patients to bear. The present article describes the treatment of two adjacent PSTDs in the forefront of the anterior region, where papilla deficiency also was exhibited. The case was treated with a coronally advanced flap and connective tissue graft (CTG) with submerged healing. The implant-supported crowns and abutments were removed, and soft-tissue augmentation was performed using a CTG that was sutured to the buccal site of the implants. The flap was released and sutured over the implants, which were submerged aiming for a closure by primary intention. After 3 months, a combination of an apically positioned flap and roll flap was performed to increase keratinized mucosa width around the implants and augment the peri-implant papilla. Clinical and esthetic satisfactory outcomes were obtained at 1 year.
Topics: Humans; Dental Implants, Single-Tooth; Treatment Outcome; Esthetics, Dental; Dental Implantation, Endosseous; Gingivoplasty; Dental Implants; Connective Tissue
PubMed: 37450675
DOI: No ID Found -
Proceedings of the National Academy of... Oct 2023In various epithelial tissues, the epithelial monolayer acts as a barrier. To fulfill its function, the structural integrity of the epithelium is tightly controlled....
In various epithelial tissues, the epithelial monolayer acts as a barrier. To fulfill its function, the structural integrity of the epithelium is tightly controlled. When normal epithelial cells detach from the basal substratum and delaminate into the apical lumen, the apically extruded cells undergo apoptosis, which is termed anoikis. In contrast, transformed cells often become resistant to anoikis and able to survive and grow in the apical luminal space, leading to the formation of multilayered structures, which can be observed at the early stage of carcinogenesis. However, the underlying molecular mechanisms still remain elusive. In this study, we first demonstrate that S100A10 and ANXA2 (Annexin A2) accumulate in apically extruded, transformed cells in both various cell culture systems and murine epithelial tissues in vivo. ANXA2 acts upstream of S100A10 accumulation. Knockdown of ANXA2 promotes apoptosis of apically extruded RasV12-transformed cells and suppresses the formation of multilayered epithelia. In addition, the intracellular reactive oxygen species (ROS) are elevated in apically extruded RasV12 cells. Treatment with ROS scavenger Trolox reduces the occurrence of apoptosis of apically extruded ANXA2-knockdown RasV12 cells and restores the formation of multilayered epithelia. Furthermore, ROS-mediated p38MAPK activation is observed in apically delaminated RasV12 cells, and ANXA2 knockdown further enhances the p38MAPK activity. Moreover, the p38MAPK inhibitor promotes the formation of multilayered epithelia of ANXA2-knockdown RasV12 cells. These results indicate that accumulated ANXA2 diminishes the ROS-mediated p38MAPK activation in apically extruded transformed cells, thereby blocking the induction of apoptosis. Hence, ANXA2 can be a potential therapeutic target to prevent multilayered, precancerous lesions.
Topics: Animals; Mice; Annexin A2; Apoptosis; Epithelial Cells; Epithelium; Reactive Oxygen Species
PubMed: 37844241
DOI: 10.1073/pnas.2307118120 -
BJOG : An International Journal of... Dec 2023Laparoscopic uterosacral ligament suspension (LUSLS) is a technique to correct apical pelvic organ prolapse (POP) by shortening the uterosacral ligaments with sutures. (Meta-Analysis)
Meta-Analysis Review
BACKGROUND
Laparoscopic uterosacral ligament suspension (LUSLS) is a technique to correct apical pelvic organ prolapse (POP) by shortening the uterosacral ligaments with sutures.
OBJECTIVE
A systematic review with meta-analysis of the effectiveness and safety of LUSLS as treatment for apical POP.
SEARCH STRATEGY
PubMed and Cochrane search using 'pelvic organ prolapse', 'laparoscopy' and 'uterosacral', including synonyms.
SELECTION CRITERIA
All articles in English presenting outcome of an original series of women with LUSLS as treatment of apical POP. Case reports were excluded.
DATA COLLECTION AND ANALYSIS
Study enrollment was performed by two reviewers. Our primary outcome measures were objective and subjective effectiveness of the procedure. Secondary outcome measures regarded complications and recurrence. Bias was assessed with the Newcastle Ottawa Scale.
MAIN RESULTS
Of 138 hits, 13 studies were included with 933 LUSLS patients. The average follow-up was 22 months. All were nonrandomised cohort studies. The pooled anatomic success rate is 90% for all LUSLS procedures (95% confidence interval [CI] 83.3-95.5). LUSLS with hysterectomy resulted in an anatomic success rate of 96.6% (95% CI 87.5-100) and LUSLS with uterus preservation 83.4% (95% CI 67.7-94.6). The pooled subjective cure rate was 90.5% (95% CI 81.9-96.5). The rate of major complications was 1%.
CONCLUSIONS
Laparoscopic uterosacral ligament suspension (with or without uterus preservation) seems to be an effective and safe treatment for women with apical POP, but long-term prospective trials and randomised controlled trials are necessary to confirm these findings.
Topics: Female; Humans; Treatment Outcome; Prospective Studies; Uterus; Pelvic Organ Prolapse; Ligaments; Laparoscopy; Gynecologic Surgical Procedures
PubMed: 37271736
DOI: 10.1111/1471-0528.17565 -
Current Problems in Cardiology Aug 2023Endomyocardial fibrosis (EMF) is an endemic idiopathic cardiomyopathy denoted by restricted and fibrous myocardium and endocardium. Apical fibrosis may cause thickening... (Review)
Review
Endomyocardial fibrosis (EMF) is an endemic idiopathic cardiomyopathy denoted by restricted and fibrous myocardium and endocardium. Apical fibrosis may cause thickening of the myocardium and extend from the ventricular apices through to the valves, thereby, restricting inflow. EMF is a critical condition that is predominant in the young population. However, it may also be associated with older individuals. Identified areas recorded with cases include the under-developed populations, that is, the tropics of Africa- Uganda, Mozambique; South Asia, and South America. Unlike in the preceded years, further studies are currently on focus to completely evaluate this medical condition. Additionally, this review encompasses the complications that arise from EMF as studied and reported from case studies, clinical trials, experiments, and research including heart failure, atrioventricular valves regurgitations, arrhythmias, serous cavities effusions, circulatory shock, stroke, myocardial ischemic syndromes, hepatic, and end-organ dysfunctions. Complications like heart failure may be very fatal to its victims. Moreover, left ventricular thrombosis may probably impose systemic or cerebral thromboembolism, which would risk the probability of a stroke. All in all, suggestions in the management strategies that have proved effective and advances in cardiac transplantation and of recently proposed therapeutic targets equally take part of this review and may form a foundation to diminishing the remaining inch of EMF and its outcomes.
Topics: Humans; Endomyocardial Fibrosis; Myocardium; Heart Failure; Heart Ventricles; Thrombosis
PubMed: 37003449
DOI: 10.1016/j.cpcardiol.2023.101730 -
The International Journal of... Jul 2023Evaluation of longitudinal strain (LS) from two-dimensional echocardiography is useful for global and regional left ventricular (LV) dysfunction assessment. We...
Evaluation of longitudinal strain (LS) from two-dimensional echocardiography is useful for global and regional left ventricular (LV) dysfunction assessment. We determined whether the LS reflects contraction process in patients with asynchronous LV activation. We studied 144 patients with an ejection fraction ≤ 35%, who had left bundle branch block (LBBB, n = 42), right ventricular apical (RVA) pacing (n = 34), LV basal- or mid-lateral pacing (n = 23), and no conduction block (Narrow-QRS, n = 45). LS distribution maps were constructed using 3 standard apical views. The times from the QRS onset-to-early systolic positive peak (Q-EPpeak) and late systolic negative peak (Q-LNpeak) were measured to determine the beginning and end of contractions in each segment. Negative strain in LBBB initially appeared in the septum and basal-lateral contracted late. In RVA and LV pacing, the contracted area enlarged centrifugally from the pacing site. Narrow-QRS showed few regional differences in strain during the systolic period. The Q-EPpeak and Q-LNpeak exhibited similar sequences characterized by septum to basal-lateral via the apical regions in LBBB, apical to basal regions in RVA pacing, and lateral to a relatively large delayed contracted area between the apical- and basal-septum in LV pacing. Differences in Q-LNpeaks between the apical and basal segments in delayed contracted wall were 107 ± 30 ms in LBBB, 133 ± 46 ms in RVA pacing, and 37 ± 20 ms in LV pacing (p < 0.05, between QRS groups). Specific LV contraction processes were demonstrated by evaluating the LS distribution and time-to-peak strain. These evaluations may have potential to estimate the activation sequence in patients with asynchronous LV activation.
Topics: Humans; Electrocardiography; Cardiac Pacing, Artificial; Predictive Value of Tests; Echocardiography; Heart Failure; Ventricular Dysfunction, Left; Bundle-Branch Block
PubMed: 36971867
DOI: 10.1007/s10554-023-02834-w