-
Journal of Clinical Neurology (Seoul,... May 2024
PubMed: 38627227
DOI: 10.3988/jcn.2023.0300 -
Indian Journal of Medical Microbiology 2024Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT) and Mycoplasma hominis (MH), the three most common treatable bacterial sexually transmitted infections (STIs)...
PURPOSE
Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT) and Mycoplasma hominis (MH), the three most common treatable bacterial sexually transmitted infections (STIs) worldwide can lead to many complications if remain untreated. Screening of high-risk population with highly sensitive methods will lead to significant improvement in patient outcomes and will prevent downward transmission. The advantages of Polymerase chain reaction (PCR) based assay are not only high sensitivity and specificity, but also detection of multiple organisms in a single reaction which reduce the result turn-around time. The aim of the present study was to evaluate the feasibility of a multiplex PCR assay method targeting 16S rRNA gene for simultaneous detection of NG, CT and MH infection along with their trend and occurrence among high-risk population in Assam, Northeast India.
METHODS
A cross-sectional study was undertaken, where a total of 200 randomly selected patients from high-risk population were included. After validation of singleplex PCR, Multiplex PCR (M-PCR) was performed along with the traditional culture method for NG.
RESULTS & CONCLUSION
The overall agreement of M-PCR with singleplex PCR was very high (100%). The occurrence of STI was found to be very high (101/200; 50.5%). Furthermore, co-infection was detected in 10/200; 5%) individuals. Infection was more common among young individuals (p < 0.05) and males out-numbered females (p < 0.05). The most common organism detected was CT (42/200; 21%) followed by NG (41/200; 20.5%) and MH (20/200; 10%). The M-PCR assay workflow is simple, cost effective and can be used in routine diagnostic laboratories with basic molecular facilities.
Topics: Humans; Sensitivity and Specificity; India; Female; Male; Adult; Cross-Sectional Studies; Chlamydia trachomatis; Neisseria gonorrhoeae; Multiplex Polymerase Chain Reaction; Young Adult; Molecular Diagnostic Techniques; RNA, Ribosomal, 16S; Sexually Transmitted Diseases, Bacterial; Polymerase Chain Reaction; Mycoplasma hominis; Adolescent; Middle Aged; Mycoplasma Infections
PubMed: 38621502
DOI: 10.1016/j.ijmmb.2024.100592 -
Orthopedics 2024Postoperative deep infection is usually identified by microbial culture. However, frequent false-negative results have severely limited effective treatment. We report a...
Postoperative deep infection is usually identified by microbial culture. However, frequent false-negative results have severely limited effective treatment. We report a rare case of intra-articular and paravertebral infection after total knee arthroplasty caused by and , with multiple negative microbial culture results. Eventually, the pathogens were identified using metagenomic high-throughput sequencing, and the patient was successfully treated with several "old" antibiotics. We analyze the clinical characteristics of this patient and systematically describe the application of high-throughput sequencing and antibiotics. [. 2024;47(1):e52-e56.].
Topics: Humans; Mycoplasma Infections; Arthroplasty, Replacement, Knee; Ureaplasma Infections; Ureaplasma urealyticum; Anti-Bacterial Agents; Postoperative Complications
PubMed: 37276443
DOI: 10.3928/01477447-20230531-04 -
Folia Microbiologica Jun 2024Following the COVID-19 infection, the sternum dislocation and wound dehiscence resulted in an infection complicating the recovery of an immunosuppressed patient after...
Following the COVID-19 infection, the sternum dislocation and wound dehiscence resulted in an infection complicating the recovery of an immunosuppressed patient after bilateral lung transplantation. Anaerobic culture (96 h) of milky cloudy wound secretion resulted in the growth of pinpoint haemolytic colonies identified as Metamycoplasma hominis (formerly Mycoplasma hominis). The search for the endogenous source of the infection found the bacterium exclusively in the patient's sputum, making a possible link to donor lung M. hominis colonization. Unfortunately, the donor samples were no longer available. The wound infection was successfully treated with 17 days of clindamycin despite the continuous PCR detection of M. hominis in the sputum after the end of the treatment.
Topics: Humans; Lung Transplantation; Surgical Wound Infection; Mycoplasma hominis; Mycoplasma Infections; Male; COVID-19; Anti-Bacterial Agents; Sputum; Middle Aged; SARS-CoV-2; Immunocompromised Host; Clindamycin
PubMed: 38625660
DOI: 10.1007/s12223-024-01160-5