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Journal of Oral Microbiology 2024Erythrosine+potassium iodide-mediated photodynamic therapy has shown an anticandidal effect. Single session, however, has inadequate fungal inhibition.
BACKGROUND
Erythrosine+potassium iodide-mediated photodynamic therapy has shown an anticandidal effect. Single session, however, has inadequate fungal inhibition.
OBJECTIVES
We aimed to examine the effects of multiple aPDT sessions on inhibition and singlet oxygen formation.
METHODS
220 μM erythrosine +/-100 mM potassium iodide was applied to biofilms for 1 min prior to irradiation at 530±10 nm using a 250 mW/cm light-emitting diode. Negative and positive controls were phosphate buffer saline and nystatin, respectively. Single, double and triple irradiation sessions with a 5 min resting time between sessions were performed. Post-treatment candidal counts were done at 0, 1 6 and 24 hr while log colony forming unit/ml was calculated and compared using a Kruskal-Wallis with Dunn's post hoc test at a <0.05 - Singlet oxygen amount was compared using one-way ANOVA with a post hoc test at a < 0.05.
RESULTS
Two and three irradiation sessions to erythrosine+potassium iodide could inhibit at 7.92 logCFU/ml ( < 0.001) . Singlet oxygen from a combination groups was significantly higher than for erythrosine (positive control). Moreover, the correlation coefficient (r) between singlet oxygen production and decreased counts was equal to 1.
CONCLUSION
Multiple sessions PDT of 220 μM erythrosine+100 mM potassium iodide effectively inhibited a biofilm.
PubMed: 38903483
DOI: 10.1080/20002297.2024.2369357 -
Nano Letters Sep 2023The rapid proliferative biological behavior of primary foci of anaplastic thyroid cancer (ATC) makes it a lethal tumor. According to the specific iodine uptake capacity...
The rapid proliferative biological behavior of primary foci of anaplastic thyroid cancer (ATC) makes it a lethal tumor. According to the specific iodine uptake capacity of thyroid cells and enhanced endocytosis of ATC cells, we designed a kind of nanoclay drug-loading system and showed a promising treatment strategy for ATC. Introducing potassium iodide (KI) improves the homoaggregation of clay nanoparticles and then affects the distribution of nanoparticles in vivo, which makes KI@DOX-Kaolin enriched almost exclusively in thyroid tissue. Simultaneously, the improvement of dispersibility of KI@DOX-Kaolin changes the target uptake of ATC cells by improving the endocytosis and nanoparticle-induced autophagy, which regulate the production of autolysosomes and autophagy-enhanced chemotherapy, eventually contributing to a tumor inhibition rate of more than 90% in the primary foci of ATC. Therefore, this facile strategy to improve the homoaggregation of nanoclay by introducing KI has the potential to become an advanced drug delivery vehicle in ATC treatment.
Topics: Humans; Thyroid Carcinoma, Anaplastic; Potassium Iodide; Kaolin; Endocytosis; Drug Delivery Systems; Thyroid Neoplasms
PubMed: 37615624
DOI: 10.1021/acs.nanolett.3c01984 -
Journal of Dentistry May 2024This study aims to evaluate antibacterial effects of silver diamine fluoride (SDF), SDF/potassium iodide (KI), and nanosilver fluoride (NSF).
OBJECTIVES
This study aims to evaluate antibacterial effects of silver diamine fluoride (SDF), SDF/potassium iodide (KI), and nanosilver fluoride (NSF).
METHODS
Antimicrobial activity of sterile saline, 5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), SDF, SDF/KI, NSF, and KI solutions against Streptococcus mutans and Lactobacillus casei was assessed through disc diffusion tests. A dual-species biofilm of S. mutans-L. casei was formed on 48 enamel samples, divided into six groups (n = 8). Group 1 was treated with sterile saline, Group 2 with 5% NaOCl, Group 3 with 2% CHX, Group 4 with SDF, Group 5 with SDF/KI, and Group 6 with NSF. The samples were analysed using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Statistical analysis utilized Shapiro-Wilk and Kruskal-Wallis tests and multiple comparisons were conducted using Dunn test.
RESULTS
SDF, SDF/KI, and NaOCl displayed significantly higher antibacterial activity against dual-species biofilm compared to NSF and CHX (p < 0.050).
CONCLUSIONS
In conclusion, SDF and SDF/KI demonstrated greater antibacterial activity than NSF. SDF's antibacterial activity was unaffected by KI. Further research is needed to determine the appropriate content and concentration for achieving effective antibacterial activity with NSF.
CLINICAL SIGNIFICANCE
The use of silver-containing materials is increasing in popularity within pediatric dentistry. In this study, an endeavor has been made to assist pediatric dentists in determining which solution might be more advantageous for preventing caries.
PubMed: 38810833
DOI: 10.1016/j.jdent.2024.105097 -
International Journal of Biological... Sep 2023In this study, the interaction between potassium iodide and protein molecules under different temperature induction was studied, taking potassium iodide (KI) and protein...
In this study, the interaction between potassium iodide and protein molecules under different temperature induction was studied, taking potassium iodide (KI) and protein molecules as a model system. The effects of KI on protein conformation, size, surface charge, binding constant, and binding site were analyzed by fluorescence spectrum, infrared spectrum, and diffusing wave spectroscopy. The results revealed that bovine serum albumin (BSA)/ovalbumin (OVA) and I formed the 1: 1 complex and significantly affected the hydrodynamic radius and spatial structure. This could be attributed to the exposure of tyrosine residues inside the proteins to the polar conditions under increased temperature. The unfolding of protein structures induced the interaction between KI/KCl and proteins. As for BSA and OVA, the particle size and surface charge of the complex increased significantly in the presence of KI/KCl. KI had a strong static quenching effect on the fluorescence of BSA and OVA. Overall, these results provide insights into the physiological effects of iodine ions.
Topics: Spectrometry, Fluorescence; Serum Albumin, Bovine; Temperature; Ovalbumin; Potassium Iodide; Muramidase; Binding Sites; Protein Binding; Spectrophotometry, Ultraviolet; Thermodynamics
PubMed: 37482161
DOI: 10.1016/j.ijbiomac.2023.125923 -
Archives of Oral Biology Aug 2024To evaluate the effect of the association of potassium iodide to antimicrobial photodynamic therapy on human carious dentin produced with a microcosm biofilm model.
OBJECTIVE
To evaluate the effect of the association of potassium iodide to antimicrobial photodynamic therapy on human carious dentin produced with a microcosm biofilm model.
METHODS
A microcosm biofilm model was used to generate a caries lesion on human dentin. Pooled human saliva diluted with glycerol was used as an inoculum on specimens immersed on McBain artificial saliva enriched with 1 % sucrose (24 h at 37 °C in 5 % CO). After refreshing culture media for 7 days, the dentin specimens were divided in 5 groups (3 specimens per group, in triplicate; n = 9): C (NaCl 0.9 %), CX (2 % chlorhexidine), PKI (0.01 % methylene blue photosensitizer+50 mM KI), L (laser at 15 J, 180 s, 22.7 J/cm), and PKIL (methylene blue + KI + Laser). After the treatments, dentin was collected, and a 10-fold serial dilution was performed. The number of total microorganisms, total lactobacilli, total streptococci, and Streptococcus mutans was analyzed by microbial counts (CFU/mL). After normality and homoscedasticity analysis, the Welch's ANOVA and Dunnett's tests were used for CFU. All tests used a 5 % significance level.
RESULTS
CX and PKIL groups showed significant bacterial decontamination of dentin, compared to group C (p < 0.05) reaching reductions up to 3.8 log for CX for all microorganisms' groups and PKIL showed 0.93, 1.30, 1.45, and 1.22 log for total microorganisms, total lactobacilli, total streptococci, and S. mutans, respectively.
CONCLUSION
aPDT mediated by the association of KI and methylene blue with red laser reduced the viability of microorganisms from carious dentin and could be a promising option for cavity decontamination.
Topics: Humans; Methylene Blue; Photochemotherapy; Dental Caries; Dentin; Potassium Iodide; Biofilms; Streptococcus mutans; Photosensitizing Agents; Saliva; Lactobacillus; Streptococcus; Chlorhexidine; In Vitro Techniques; Colony Count, Microbial; Saliva, Artificial; Lasers
PubMed: 38718465
DOI: 10.1016/j.archoralbio.2024.105978 -
Nanomaterials (Basel, Switzerland) Jun 2024This work highlights the novel approach of incorporating potassium iodide (KI) doping during the synthesis of InP core quantum dots (QDs) to significantly reduce the...
This work highlights the novel approach of incorporating potassium iodide (KI) doping during the synthesis of InP core quantum dots (QDs) to significantly reduce the concentration of vacancies (i.e., In vacancies; ) within the bulk of the core QD and inhibit the formation of InPO at the core QD-ZnSe shell interfaces. The photoluminescence quantum yield (PLQY) of ~97% and full width at half maximum (FWHM) of ~40 nm were achieved for InP/ZnSe/ZnSeS/ZnS core/multi-shell QDs emitting red light, which is essential for a quantum-dot organic light-emitting diode (QD-OLED) without red, green, and blue crosstalk. KI doping eliminated in the core QD bulk by forming K- substitutes and effectively inhibited the formation of InPO(HO) at the core QD-ZnSe shell interface through the passivation of phosphorus (P)-dangling bonds by P-I bonds. The elimination of vacancies in the core QD bulk was evidenced by the decreased relative intensity of non-radiative unpaired electrons, measured by electron spin resonance (ESR). Additionally, the inhibition of InPO(HO) formation at the core QD and shell interface was confirmed by the absence of the {210} X-ray diffraction (XRD) peak intensity for the core/multi-shell QDs. By finely tuning the doping concentration, the optimal level was achieved, ensuring maximum K- substitution, minimal K and I interstitials, and maximum P-dangling bond passivation. This resulted in the smallest core QD diameter distribution and maximized optical properties. Consequently, the maximum PLQY (~97%) and minimum FWHM (~40 nm) were observed at 3% KI doping. Furthermore, the color gamut of a QD-OLED display using R-, G-, and B-QD functional color filters (i.e., ~131.1%@NTSC and [email protected]) provided a nearly perfect color representation, where red-light-emitting KI-doped QDs were applied.
PubMed: 38921931
DOI: 10.3390/nano14121055 -
Chemical Record (New York, N.Y.) Oct 2023The catalytic potential of organo-onium iodides as nucleophilic catalysts is aptly demonstrated in the synthesis of cyclic carbonates from epoxides and carbon dioxide... (Review)
Review
The catalytic potential of organo-onium iodides as nucleophilic catalysts is aptly demonstrated in the synthesis of cyclic carbonates from epoxides and carbon dioxide (CO ), as a representative CO utilization reaction. Although organo-onium iodide nucleophilic catalysts are metal-free environmentally benign catalysts, harsh reaction conditions are generally required to efficiently promote the coupling reactions of epoxides and CO . To solve this problem and accomplish efficient CO utilization reactions under mild conditions, bifunctional onium iodide nucleophilic catalysts bearing a hydrogen bond donor moiety were developed by our research group. Based on the successful bifunctional design of the onium iodide catalysts, nucleophilic catalysis using a potassium iodide (KI)-tetraethylene glycol complex was also investigated in coupling reactions of epoxides and CO under mild reaction conditions. These effective bifunctional onium and potassium iodide nucleophilic catalysts were applied to the solvent-free syntheses of 2-oxazolidinones and cyclic thiocarbonates from epoxides.
PubMed: 37236152
DOI: 10.1002/tcr.202300144 -
Frontiers in Microbiology 2023In this the antibacterial of quercetin against was evaluated by measuring the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC)....
In this the antibacterial of quercetin against was evaluated by measuring the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Subsequently, the effect of quercetin on cell membrane was evaluated through scanning electron microscopy (SEM), surface hydrophobicity determination, diacetate fluorescein staining and propidium iodide (PI) staining. Additionally, the effects of quercetin on intracellular macromolecules and cell metabolism were explored by measuring the culture medium protein, bacterial protein and intracellular sodium and potassium adenosine triphosphate (ATP) enzyme activity. The results revealed that quercetin exhibited the MIC and MBC values of 100 ug/mL and 400 ug/mL, respectively, against . The SEM results revealed that quercetin could induce irreversible damage to the cell membrane effectively. Moreover, quercetin could enhance the surface hydrophobicity of . The results of flow cytometry and fluorescence microscopy analyses revealed that quercetin could promote cell damage by altering the cell membrane permeability of , inducing the release of nucleic acid substances from the cells. Furthermore, the determination of protein content in the culture medium, bacterial protein content, and the Na(+)/K(+)-ATPase activity demonstrated that quercetin could reduce the intracellular protein content and impedes protein expression and ATPase synthesis effectively, leading to apoptosis.
PubMed: 38033555
DOI: 10.3389/fmicb.2023.1286187 -
Journal of Fungi (Basel, Switzerland) Jan 2024Feline sporotrichosis is an endemic disease with high occurrence in Brazil. Itraconazole (ITZ) remains the drug of choice for treating this disease in cats, despite the...
Feline sporotrichosis is an endemic disease with high occurrence in Brazil. Itraconazole (ITZ) remains the drug of choice for treating this disease in cats, despite the increasing reports of therapeutic failure. A controlled, randomized clinical trial was performed on 166 naive cats with sporotrichosis to assess the effectiveness and safety of the combination therapy with ITZ and potassium iodide (KI) compared with ITZ monotherapy. Cats were randomly allocated into two treatment groups: G1-ITZ 100 mg/cat/day-and G2-ITZ 100 mg/cat/day + KI 2.5-20 mg/kg/day. Cats treated in G2 presented 77% more risk of reaching a clinical cure (a positive effect) than those treated in G1, even when controlled by negative predictors. The survival curves of the two treatment protocols indicate that a clinical cure was achieved faster in G2. An increase in the KI dose was necessary in 28 cats due to the persistence of clinical signs. Adverse reactions were equally frequent in both groups and manageable with a temporary drug suspension and/or a hepatoprotective therapy. The combination therapy was associated with a higher cure rate and a shorter treatment time, suggesting that ITZ+KI arises as a better option for treating feline sporotrichosis and should be considered the first-line treatment, especially in the presence of negative predictors.
PubMed: 38392773
DOI: 10.3390/jof10020101 -
Cureus Dec 2023Objectives This study aimed to evaluate the antibiofilm effect of calcium hydroxide (CH), 0.5% iodine potassium iodide (IKI), and 0.5% cetrimide (CTR), alone and in...
Objectives This study aimed to evaluate the antibiofilm effect of calcium hydroxide (CH), 0.5% iodine potassium iodide (IKI), and 0.5% cetrimide (CTR), alone and in combinations on one-week and three-week-old biofilms. Materials and methods Gingival plaque was collected, and biofilms were grown anaerobically. Biofilms were exposed to each of the three medicaments and their combinations for one day, one week, and two weeks. Proportions of dead and live bacteria in the biofilms were evaluated. Results The killing of bacteria by different medicaments in the three-week-old biofilm was lower than in the one-week-old biofilm (<0.05). The efficacy of IKI and CTR in killing bacteria was weaker than that of CH, but the highest efficacy in killing was achieved when all three were combined (<0.05). There was no significant difference in the antibiofilm effect between a day's exposure to the mixture of the three medicaments and one or two weeks of treatment with CH alone (>0.05). Conclusions Three-week-old biofilms are more resistant to medicaments than one-week-old biofilms. Combining IKI and CTR with CH resulted in a stronger antibiofilm effect than using CH alone. Mixing the three medicaments may enable obtaining the desired clinical effect in a shorter exposure time.
PubMed: 38283497
DOI: 10.7759/cureus.51203