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Environmental Science & Technology Dec 2023The co-occurrence of uranyl and arsenate in contaminated water caused by natural processes and mining is a concern for impacted communities, including in Native American...
The co-occurrence of uranyl and arsenate in contaminated water caused by natural processes and mining is a concern for impacted communities, including in Native American lands in the U.S. Southwest. We investigated the simultaneous removal of aqueous uranyl and arsenate after the reaction with limestone and precipitated hydroxyapatite (HAp, Ca(PO)(OH)). In benchtop experiments with an initial pH of 3.0 and initial concentrations of 1 mM U and As, uranyl and arsenate coprecipitated in the presence of 1 g L limestone. However, related experiments initiated under circumneutral pH conditions showed that uranyl and arsenate remained soluble. Upon addition of 1 mM PO and 3 mM Ca in solution (initial concentration of 0.05 mM U and As) resulted in the rapid removal of over 97% of U via Ca-U-P precipitation. In experiments with 2 mM PO and 10 mM Ca at pH rising from 7.0 to 11.0, aqueous concentrations of As decreased (between 30 and 98%) circa pH 9. HAp precipitation in solids was confirmed by powder X-ray diffraction and scanning electron microscopy/energy dispersive X-ray. Electron microprobe analysis indicated U was coprecipitated with Ca and P, while As was mainly immobilized through HAp adsorption. The results indicate that natural materials, such as HAp and limestone, can effectively remove uranyl and arsenate mixtures.
Topics: Arsenates; Calcium Carbonate; Hydrogen-Ion Concentration; Adsorption; Water; Uranium
PubMed: 38019567
DOI: 10.1021/acs.est.3c03809 -
Molecules (Basel, Switzerland) Aug 2023Bismuth-based drugs have been used primarily to treat ulcers caused by and other gastrointestinal ailments. Combined with antibiotics, these drugs also possess... (Review)
Review
Bismuth-based drugs have been used primarily to treat ulcers caused by and other gastrointestinal ailments. Combined with antibiotics, these drugs also possess synergistic activity, making them ideal for multiple therapy regimens and overcoming bacterial resistance. Compounds based on bismuth have a low cost, are safe for human use, and some of them are also effective against tumoral cells, leishmaniasis, fungi, and viruses. However, these compounds have limited bioavailability in physiological environments. As a result, there is a growing interest in developing new bismuth compounds and approaches to overcome this challenge. Considering the beneficial properties of bismuth and the importance of discovering new drugs, this review focused on the last decade's updates involving bismuth compounds, especially those with potent activity and low toxicity, desirable characteristics for developing new drugs. In addition, bismuth-based compounds with dual activity were also highlighted, as well as their modes of action and structure-activity relationship, among other relevant discoveries. In this way, we hope this review provides a fertile ground for rationalizing new bismuth-based drugs.
Topics: Humans; Bismuth; Anti-Bacterial Agents; Helicobacter pylori; Helicobacter Infections; Drug Therapy, Combination
PubMed: 37570891
DOI: 10.3390/molecules28155921 -
International Journal of Molecular... Jul 2023The introduction of the notion of energy change resulting from the ion exchange in apatites leads to the question: how can some simple isomorphic series be described...
The introduction of the notion of energy change resulting from the ion exchange in apatites leads to the question: how can some simple isomorphic series be described using the mentioned idea? We concentrated on the simple isomorphic series of compounds: apatite, bioapatite, calcite, aragonite, celestine, K-, Zn- and Cu-Tutton's salts. It was demonstrated in all the series, except Tutton's salts, that the change in energy and the change in the crystal cell volume are, in a simple way, dependent on the change in the ionic radii of the introduced ions. The linear relationships between the variations in energy and in the universal crystallographic dimension d were derived from the earlier equations and proven based on available data. In many cases, except the Tutton's salts, linear dependence was discovered between the change in energy and the sinus of universal angle Θ, corresponding to the change in momentum transfer. In the same cases, linear dependencies were observed between the energy changes and the changes in the volumes of crystallographic cells, and mutually between changes in the crystallographic cell volume V, crystallographic dimension d, and diffraction angle Θ.
Topics: Salts; Crystallography; Ions; Calcium Carbonate; Apatites; X-Ray Diffraction
PubMed: 37511085
DOI: 10.3390/ijms241411324 -
BMC Gastroenterology Jul 2023This study aimed to evaluate the efficacy, adverse events, patient compliance, and cost of dual therapy with Ilaprazole-amoxicillin (IA) at high dose versus... (Randomized Controlled Trial)
Randomized Controlled Trial
Ilaprazole-amoxicillin dual therapy at high dose as a first-line treatment for helicobacter pylori infection in Hainan: a single-center, open-label, noninferiority, randomized controlled trial.
OBJECTIVES
This study aimed to evaluate the efficacy, adverse events, patient compliance, and cost of dual therapy with Ilaprazole-amoxicillin (IA) at high dose versus Ilaprazole-amoxicillin-furazolidone-bismuth (IAFB) quadruple therapy for the Helicobacter pylori (H.pylori) infection among Chinese patients.
METHODS
200 patients who had tested positive for H. pylori and undergoing upper gastrointestinal endoscopy after being diagnosed with chronic gastritis participated in this open-label randomized controlled clinical trial. Patients were randomized to Group A and Group B: the 14-day IA dual treatment group (101) and IAFB quadruple treatment group (99). The C urea breath test was conducted to determine whether H. pylori had been eliminated 4-6 weeks after the treatment. Eradication rates, drug-related adverse events, patient compliance, and drug costs were compared between the two treatment groups.
RESULTS
Eradication rates in group A were 92.1% and 94.9%, depending on the intention-to-treat (ITT), per-protocol (PP), respectively, which was similar to group B (91.9% and 93.6%). There was no significant difference observed in adverse events between the two groups (P = 0.518). Interestingly, compliance was significantly higher in group A compared to the group B (P = 0.031). In addition, drug costs were significantly lower for group A in comparison to the group B.
CONCLUSIONS
IA dual therapy was found to be equally effective, safer and less costly than IAFB quadruple therapy. Therefore, these therapies can be potentially considered as first-line regimens for empirical treatment.
Topics: Humans; Helicobacter Infections; Amoxicillin; Helicobacter pylori; 2-Pyridinylmethylsulfinylbenzimidazoles; Bismuth; Furazolidone
PubMed: 37488516
DOI: 10.1186/s12876-023-02890-5 -
International Journal of Molecular... Jun 2023Hybrid nanocomposite materials BuNBF-MgO were obtained using a nanocrystalline MgO with a specific surface area of 324 m/g and the grains size of 5.1 nm. As a result of...
Hybrid nanocomposite materials BuNBF-MgO were obtained using a nanocrystalline MgO with a specific surface area of 324 m/g and the grains size of 5.1 nm. As a result of the strong adhesion, the salt transforms into an interface-stabilized amorphous state within the thin layer near the interface. The analysis of the DSC data allowed one to estimate the concentration and the thickness of this amorphous layer as 4.8 nm. The amorphous interface phase has an enhanced ionic conductivity. As a result, conductivity of the nanocomposite increases with the concentration of the amorphous phase and reaches 1.1 × 10 S/cm at 150 °C at a concentration of the MgO additive x = 0.90 corresponding to the maximum content of the amorphous phase. The conductivity of the nanocomposite is by three orders of magnitude higher than the conductivity of pure BuNBF. The nanocomposites are electrochemically stable up to 2.5 V. At high concentrations of MgO when the total volume of the salt is small the composites become nano- and mesoporous.
Topics: Magnesium Oxide; Nanocomposites; Electrolytes; Ions
PubMed: 37446124
DOI: 10.3390/ijms241310949 -
The Korean Journal of Internal Medicine Jan 2024We evaluated the efficacy and safety of denosumab treatment in severe chronic kidney disease (CKD) patients with osteoporosis. We also investigated whether the treatment...
BACKGROUND/AIMS
We evaluated the efficacy and safety of denosumab treatment in severe chronic kidney disease (CKD) patients with osteoporosis. We also investigated whether the treatment affects the coronary artery calcifications.
METHODS
Twenty-seven postmenopausal women with Stage 3b-4 CKD and osteoporosis were enrolled. Twenty patients received denosumab plus calcium carbonate and vitamin D, and seven controls received calcium carbonate and vitamin D for 1 year. Dual-energy X-ray absorptiometry and coronary artery calcium (CAC) scoring computed tomography were performed before and after treatment. Hypocalcemic symptoms and serum calcium levels were evaluated.
RESULTS
After 1 year of treatment, the percent changes of femur neck (3.6 ± 3.2% vs. -0.7 ± 4.4%, p = 0.033) and total hip (3.4 ± 3.8% vs. -1.9 ± 2.1%, p = 0.001) bone mineral density (BMD) were significantly increased in the denosumab treated group compared to the control group. However, the percent change of lumbar spine BMD did not differ between two groups (5.6 ± 5.9% vs. 2.7 ± 3.9%, p = 0.273). The percent change of bone alkaline phosphatase was significantly different in the denosumab-treated group and control group (-31.1 ± 30.0% vs. 0.5 ± 32.0%, p = 0.027). CAC scores did not differ between groups. No hypocalcemic events occurred in both groups.
CONCLUSION
If carefully monitored and supplemented with calcium and vitamin D, denosumab treatment for 1 year provides significant benefits in patients with Stage 3b-4 CKD and osteoporosis. However, denosumab treatment did not affect coronary artery calcifications in these patients.
Topics: Humans; Female; Denosumab; Bone Density Conservation Agents; Calcium; Osteoporosis; Bone Density; Vitamin D; Renal Insufficiency, Chronic; Calcium Carbonate; Republic of Korea; Osteoporosis, Postmenopausal
PubMed: 38145616
DOI: 10.3904/kjim.2023.292 -
BMC Medicine Apr 2024The stalling global progress in malaria control highlights the need for novel tools for malaria elimination, including transmission-blocking vaccines.... (Randomized Controlled Trial)
Randomized Controlled Trial
BACKGROUND
The stalling global progress in malaria control highlights the need for novel tools for malaria elimination, including transmission-blocking vaccines. Transmission-blocking vaccines aim to induce human antibodies that block parasite development in the mosquito and mosquitoes becoming infectious. The Pfs48/45 protein is a leading Plasmodium falciparum transmission-blocking vaccine candidate. The R0.6C fusion protein, consisting of Pfs48/45 domain 3 (6C) and the N-terminal region of P. falciparum glutamate-rich protein (R0), has previously been produced in Lactococcus lactis and elicited functional antibodies in rodents. Here, we assess the safety and transmission-reducing efficacy of R0.6C adsorbed to aluminium hydroxide with and without Matrix-M™ adjuvant in humans.
METHODS
In this first-in-human, open-label clinical trial, malaria-naïve adults, aged 18-55 years, were recruited at the Radboudumc in Nijmegen, the Netherlands. Participants received four intramuscular vaccinations on days 0, 28, 56 and 168 with either 30 µg or 100 µg of R0.6C and were randomised for the allocation of one of the two different adjuvant combinations: aluminium hydroxide alone, or aluminium hydroxide combined with Matrix-M1™ adjuvant. Adverse events were recorded from inclusion until 84 days after the fourth vaccination. Anti-R0.6C and anti-6C IgG titres were measured by enzyme-linked immunosorbent assay. Transmission-reducing activity of participants' serum and purified vaccine-specific immunoglobulin G was assessed by standard membrane feeding assays using laboratory-reared Anopheles stephensi mosquitoes and cultured P. falciparum gametocytes.
RESULTS
Thirty-one participants completed four vaccinations and were included in the analysis. Administration of all doses was safe and well-tolerated, with one related grade 3 adverse event (transient fever) and no serious adverse events occurring. Anti-R0.6C and anti-6C IgG titres were similar between the 30 and 100 µg R0.6C arms, but higher in Matrix-M1™ arms. Neat participant sera did not induce significant transmission-reducing activity in mosquito feeding experiments, but concentrated vaccine-specific IgGs purified from sera collected two weeks after the fourth vaccination achieved up to 99% transmission-reducing activity.
CONCLUSIONS
R0.6C/aluminium hydroxide with or without Matrix-M1™ is safe, immunogenic and induces functional Pfs48/45-specific transmission-blocking antibodies, albeit at insufficient serum concentrations to result in transmission reduction by neat serum. Future work should focus on identifying alternative vaccine formulations or regimens that enhance functional antibody responses.
TRIAL REGISTRATION
The trial is registered with ClinicalTrials.gov under identifier NCT04862416.
Topics: Adolescent; Adult; Animals; Female; Humans; Male; Middle Aged; Young Adult; Adjuvants, Immunologic; Aluminum Hydroxide; Antibodies, Protozoan; Malaria Vaccines; Malaria, Falciparum; Membrane Glycoproteins; Netherlands; Plasmodium falciparum; Protozoan Proteins
PubMed: 38649867
DOI: 10.1186/s12916-024-03379-y -
Journal of the Mechanical Behavior of... Jul 2024Living organisms form complex mineralized composite architectures that perform a variety of essential functions. These materials are commonly utilized for load-bearing...
Living organisms form complex mineralized composite architectures that perform a variety of essential functions. These materials are commonly utilized for load-bearing purposes such as structural stability and mechanical strength in combination with high toughness and deformability, which are well demonstrated in various highly mineralized molluscan shell ultrastructures. Here, the mineral components provide the general stiffness to the composites, and the organic interfaces play a key role in providing these biogenic architectures with mechanical superiority. Although numerous studies employed state-of-the-art methods to measure and/or model and/or simulate the mechanical behavior of molluscan shells, our understanding of their performance is limited. This is partially due to the lack of the most fundamental knowledge of their mechanical characteristics, particularly, the anisotropic elastic properties of the mineral components and of the tissues they form. In fact, elastic constants of biogenic calcium carbonate, one of the most common biominerals in nature, is unknown for any organism. In this work, we employ the ultrasonic pulse-echo method to report the elasticity tensor of two common ultrastructural motifs in molluscan shells: the prismatic and the nacreous architectures made of biogenic calcite and aragonite, respectively. The outcome of this research not only provides information necessary for fundamental understanding of biological materials formation and performance, but also yields textbook knowledge on biogenic calcium carbonate required for future structural/crystallographic, theoretical and computational studies.
Topics: Calcium Carbonate; Elasticity; Animal Shells; Animals; Materials Testing; Mollusca; Biomechanical Phenomena; Nacre
PubMed: 38762971
DOI: 10.1016/j.jmbbm.2024.106570 -
PloS One 2023The immovable nature of built heritage means that it is particularly vulnerable during times of armed conflict. Although impacts from small arms and shrapnel leave...
The immovable nature of built heritage means that it is particularly vulnerable during times of armed conflict. Although impacts from small arms and shrapnel leave relatively inconspicuous impact scars, they elevate the risk of future stone deterioration. This study investigates the subsurface damage caused by bullet impacts, which is not apparent from surface inspection, in order to better understand the geometry and mechanics of this form of conflict damage to heritage. Controlled firearm experiments were conducted to simulate conflict damage to sandstone and limestone buildings. The bullet impacts created conical fractures or zones of increased fracture intensity below the impact, radial fractures, and spallation, in addition to a crater. Dynamic fracture distinguishes the formation of these features from quasi static cone crack experiments, while the lack of a shockwave differentiates these bullet impacts from hypervelocity experiments. Damage was created by momentum transfer from the bullet, so that differences in target properties had large effects on the nature of the damage. The crater in the limestone target was almost an order of magnitude deeper than the sandstone crater, and large open fractures formed in the limestone below the crater floor, compared with zones of increased fracture intensity in the sandstone target. Microstructural analysis of subsurface damage showed that fracture intensity decreased with increasing distance from the impact centre, suggesting that regions proximal to the impact are at increased risk of future deterioration. Conical subsurface fractures dipping away from the impact beneath multiple impact craters could link up, creating a continuous fracture network. By providing pathways for moisture and other weathering agents, fractures enlarge the region at increased risk of deterioration. Their lack of surface expression makes understanding their formation a vital part of future surveying and post conflict assessments.
Topics: Humans; Fractures, Bone; Firearms; Calcium Carbonate; Weather
PubMed: 37878563
DOI: 10.1371/journal.pone.0292351 -
Molecules (Basel, Switzerland) Jan 2024The misuse of growth-promoting drugs such as beta-2 agonists and steroids is a known problem in farming and sports competitions. Prior to the analysis of biological... (Review)
Review
The misuse of growth-promoting drugs such as beta-2 agonists and steroids is a known problem in farming and sports competitions. Prior to the analysis of biological samples via liquid chromatography (LC)-mass spectrometry (MS) or gas chromatography (GC)-MS, sufficient sample preparation is required to reliably identify or determine the residues of drugs. In practice, broad screening methods are often used to save time and analyze as many compounds as possible. This review was conceptualized to analyze the literature from 2018 until October 2023 for sample preparation procedures applied to animal specimens before LC- or GC-MS analysis. The animals were either used in farming or sports. In the present review, solid phase extraction (SPE) was observed as the dominant sample clean-up technique for beta-2 agonists and steroids, followed by protein precipitation. For the extraction of beta-2 agonists, mixed-mode cation exchanger-based SPE phases were preferably applied, while for the steroids, various types of SPE materials were reported. Furthermore, dispersive SPE-based QuEChERs were utilized. Combinatory use of SPE and liquid-liquid extraction (LLE) was observed to cover further drug classes in addition to beta-2 agonists in broader screening methods.
Topics: Animals; Agriculture; Farms; Anesthetics, Local; Antacids; Intercellular Signaling Peptides and Proteins; Mammals; Steroids
PubMed: 38257243
DOI: 10.3390/molecules29020330