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Infection and Drug Resistance 2024In recent years, has gained attention for its rare occurrence in patient infections. The literature consists mostly of case reports, necessitating further research to...
BACKGROUND
In recent years, has gained attention for its rare occurrence in patient infections. The literature consists mostly of case reports, necessitating further research to comprehensively understand risk factors, clinical characteristics, and management strategies.
METHODS
We conducted a retrospective cohort study involving patients diagnosed with infection at a tertiary teaching hospital between January 2014 and December 2022. The data included demographic details, comorbidities, bacterial cultures, antibiotic susceptibility, and treatment outcomes.
RESULTS
There were 26 patients diagnosed with infection who were predominantly older with multiple comorbidities. Approximately 76.9% of infection patients had polymicrobial infections. Twenty-one patients had received antibiotics within three months before they developed the infection, and these antibiotics were primarily third-generation cephalosporins, glycopeptides and fluoroquinolones. Antibiotic susceptibility testing showed resistance to aminoglycosides and susceptibility to imipenem, meropenem, ceftazidime, and piperacillin/tazobactam. Treatment outcome showed a mortality rate of 11.5%, mainly in patients with malignancy and advanced age.
CONCLUSION
infections predominantly affect older patients with multiple comorbidities. In terms of antibiotic therapy, carbapenems, cephalosporins, and piperacillin/tazobactam with antipseudomonal activity could all be considered.
PubMed: 38736436
DOI: 10.2147/IDR.S457781 -
Animals : An Open Access Journal From... Dec 2023The present study was carried out to determine the seminal microbiota of boars and their correlation with sperm quality. A total of 17 ejaculates were collected from 17...
The present study was carried out to determine the seminal microbiota of boars and their correlation with sperm quality. A total of 17 ejaculates were collected from 17 Duroc boars and were classified according to sperm quality into two groups: low-quality ( = 8) and high-quality ( = 9). Each ejaculate was subjected to (i) semen evaluation, (ii) bacterial culture and MALDI-TOF identification, and (iii) 16S rRNA gene sequencing and bioinformatic analyses. No difference in the total bacterial count, alpha diversity, and beta diversity between the high-quality group and the low-quality group was detected ( > 0.05). While was negatively correlated with sperm quality ( < 0.05), was positively correlated with sperm quality ( < 0.05). Lactobacillales (25.2%; LB) and Enterobacterales (10.3%; EB) were the most dominant bacteria and negatively correlated: EB = 507.3 - 0.5 × LB, R = 0.24, < 0.001. Moreover, the abundance of was negatively correlated with LB (r = -0.754, < 0.001) and positively correlated with (r = 0.533, < 0.05). was positively correlated with (r = 0.485, < 0.05), (r = 0.622, < 0.01), and (r = 0.489, < 0.05). In conclusion, seminal microbiota is significantly associated with boar semen qualities. The distributions of the most dominant bacterial genera, the differences in the abundance of small subset microbes, and their correlation appear to have far more impact than the overall seminal bacterial content (e.g., total bacterial count, alpha diversity, and beta diversity) on sperm quality.
PubMed: 38136874
DOI: 10.3390/ani13243837 -
Journal of Global Infectious Diseases 2023is an aerobic, nonfermentative Gram-negative bacillus infrequently isolated from clinical specimens. The pathogenicity and clinical significance of the organism has not...
is an aerobic, nonfermentative Gram-negative bacillus infrequently isolated from clinical specimens. The pathogenicity and clinical significance of the organism has not been ascertained due to uncommon clinical isolation and suspected low virulence. The organism has been reported to be inherently resistant to aminoglycoside group of drugs which remain as a widely used first-line drug of choice for febrile neutropenic patients. Hereby, we report a case of associated pleural effusion in a patient of metastatic adenocarcinoma diagnosed and treated timely and successfully with appropriate antibiotics.
PubMed: 37800087
DOI: 10.4103/jgid.jgid_66_22 -
Frontiers in Cellular and Infection... 2023Healthy lung microbiota plays an important role in preventing (Mtb) infections by activating immune cells and stimulating production of T-helper cell type 1 cytokines....
INTRODUCTION
Healthy lung microbiota plays an important role in preventing (Mtb) infections by activating immune cells and stimulating production of T-helper cell type 1 cytokines. The dynamic stability of lung microbiota relies mostly on lung homeostasis. In our previous studies, we found that Mtb virulence factor, Rv1987 protein, can mediate host immune response and enhance mycobacterial survival in host lung. However, the alteration of lung microbiota and the contribution of lung microbiota dysbiosis to mycobacterial evasion in this process are not clear so far.
METHODS
which does not contain the ortholog of Rv1987 protein was selected as a model strain to study the effects of Rv1987 on host lung microbiota. The lung microbiota, immune state and metabolites of mice infected by overexpressing Rv1987 protein (MS1987) were detected and analyzed.
RESULTS
The results showed that Rv1987 inhibited inflammatory response in mouse lung and anaerobic bacteria and , , and bacteria were enriched in the lung tissues correspondingly. The immune alterations and microbiota dysbiosis affected host metabolic profiles, and some of significantly altered bacteria in MS1987-infected mouse lung, such as , and , led to anti-inflammatory responses in mouse lung. The secretory metabolites of these altered bacteria also influenced mycobacterial growth and biofilm formation directly.
CONCLUSION
All these results suggested that Rv1987 can attenuate inflammatory response and alter microbiota in the lung, which in turn facilitates mycobacterial survival in the host.
Topics: Animals; Mice; Mycobacterium tuberculosis; Dysbiosis; Cytokines; Mycobacterium Infections; Lung; Microbiota
PubMed: 38029253
DOI: 10.3389/fcimb.2023.1256866 -
Microbial Ecology Nov 2023Agricultural crops are exposed to various abiotic and biotic stresses that can constrain crop productivity. Focusing on a limited subset of key groups of organisms has...
Agricultural crops are exposed to various abiotic and biotic stresses that can constrain crop productivity. Focusing on a limited subset of key groups of organisms has the potential to facilitate the monitoring of the functions of human-managed ecosystems. Endophytic bacteria can enhance plant stress resistance and can help plants to cope with the negative impacts of stress factors through the induction of different mechanisms, influencing plant biochemistry and physiology. In this study, we characterise endophytic bacteria isolated from different plants based on their metabolic activity and ability to synthesise 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD), the activity of hydrolytic exoenzymes, the total phenolic compounds (TPC) and iron-complexing compounds (ICC). Test GEN III MicroPlate indicated that the evaluated endophytes are highly metabolically active, and the best used substrates were amino acids, which may be important in selecting potential carrier components for bacteria in biopreparations. The ACCD activity of strain ES2 (Stenotrophomonas maltophilia) was the highest, whereas that of strain ZR5 (Delftia acidovorans) was the lowest. Overall, the obtained results indicated that ∼91.3% of the isolates were capable of producing at least one of the four hydrolytic enzymes. In addition, most of the tested strains produced ICC and TPC, which play a significant role in reducing stress in plants. The results of this study suggest that the tested endophytic bacterial strains can potentially be used to mitigate climate change-associated stresses in plants and to inhibit plant pathogens.
Topics: Humans; Ecosystem; Bacteria; Endophytes; Crops, Agricultural; Stress, Physiological
PubMed: 37392205
DOI: 10.1007/s00248-023-02260-4 -
Pathogens (Basel, Switzerland) Nov 2023Styles transmit pollen-derived sperm nuclei from pollen to ovules, but also transmit environmental pathogens. The microbiomes of styles are likely important for...
Styles transmit pollen-derived sperm nuclei from pollen to ovules, but also transmit environmental pathogens. The microbiomes of styles are likely important for reproduction/disease, yet few studies exist. Whether style microbiome compositions are spatially responsive to pathogens is unknown. The maize pathogen enters developing grain through the style (silk). We hypothesized that treatment shifts the cultured transmitting silk microbiome (TSM) compared to healthy silks in a distance-dependent manner. Another objective of the study was to culture microbes for future application. Bacteria were cultured from husk-covered silks of 14 -treated diverse maize genotypes, proximal (tip) and distal (base) to the inoculation site. Long-read 16S sequences from 398 isolates spanned 35 genera, 71 species, and 238 OTUs. More bacteria were cultured from -inoculated tips (271 isolates) versus base (127 isolates); healthy silks were balanced. caused a collapse in diversity of ~20-25% across multiple taxonomic levels. Some species were cultured exclusively or, more often, from -treated silks (e.g., , , , , ). Overall, the results suggest that alters the TSM in a distance-dependent manner. Many isolates matched taxa that were previously identified using V4-MiSeq (core and -induced), but long-read sequencing clarified the taxonomy and uncovered greater diversity than was initially predicted (e.g., within ). These isolates represent the first comprehensive cultured collection from pathogen-treated maize silks to facilitate biocontrol efforts and microbial marker-assisted breeding.
PubMed: 38003787
DOI: 10.3390/pathogens12111322 -
Microbiology Resource Announcements Jan 2024Soil is a source for diverse microbes that possess useful biotechnological capabilities. Here, we report the genome sequences of seven bacterial isolates from the...
Soil is a source for diverse microbes that possess useful biotechnological capabilities. Here, we report the genome sequences of seven bacterial isolates from the species , , , , sp., and (two isolates) cultured from Dallas/Fort Worth metroplex soil samples.
PubMed: 38038466
DOI: 10.1128/MRA.00357-23