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Frontiers in Physiology 2023Declines in honeybee abundance have been observed worldwide during last decades. This is partly due to plant protection agents used in intensive farming, landscaping...
Declines in honeybee abundance have been observed worldwide during last decades. This is partly due to plant protection agents used in intensive farming, landscaping and infrastructure maintenance. Another type of factors negatively affecting honeybees is the spread of diseases caused by different pathogens and pests. Lately, more focus has been paid to the interactions between different overlapping stressors affecting honeybee health, the combination of these often being more detrimental compared to individual stressors. The most widely used stress-evaluating methods take into account lethal- or motorial changes of the individuals or colonies. Comparatively little honeybee research has examined changes in initial recovery potential and physiological symptoms of toxification. The aim of this study was to examine the combined effect of and (according to a newer classification and ), the common causes of nosemosis in the honeybee L., with the insecticide dimethoate. In this study, honeybee mortality and metabolic rate were used to assess the combined effects interactions of ssp. and dimethoate. Our results showed that exposure to the low concentration of either dimethoate, either one or both species of ssp as single factors or in the combination had no significant effect on honeybee metabolic rate. The mortality increased with the two spp., as well as with infection by alone. The effect of dimethoate was observed only in combination with infection, which alone had no effect on individual honeybee mortality. This study demonstrates that the overlapping exposure to a non-lethal concentration of a pesticide and a pathogen can be hidden by stronger stressor but become observable with milder stressors.
PubMed: 37346484
DOI: 10.3389/fphys.2023.1198070 -
Anais Da Academia Brasileira de Ciencias 2023Although it is known that organophosphate insecticides are harmfull to aquatic ecosystems, oxidative damages caused by Dimethoate and Chlorpyrifos are not studied on...
Although it is known that organophosphate insecticides are harmfull to aquatic ecosystems, oxidative damages caused by Dimethoate and Chlorpyrifos are not studied on Arthrospira platensis Gomont. In this study, various Chlorpyrifos (0-150 µg mL-1) and Dimethoate (0-250 µg mL-1) concentrations were added to the culture medium in laboratory to evaulate growth rate, chlorophyll-a content and antioxidant parameters of A. platensis. Optical Density (OD560) and chlorophyll-a decreased compared to the control for seven days in both pesticide applications. Superoxide dismutase (SOD) activity increased at 50 µg mL-1 Chlorpyrifos concentration but it decreased at all concentrations. Although Ascorbate peroxidase (APX) and glutathione reductase (GR) activities increased with Chlorpyrifos application, they did not change with Dimethoate application. Malondialdehyde (MDA) amount decreased at 150 µg mL-1 Chlorpyrifos concentration but it increased in Dimethoate application. The H2O2 content were increased in both applications. Proline decreased in 50 and 75 µg mL-1 Chlorpyrifos concentrations and increased at 150 µg mL-1 concentration, while it increased at 25 µg mL-1 Dimethoate concentration. The results were tested at 0.05 significance level. These pesticides inhibit A. platensis growth and chlorophyll-a production and cause oxidative stress. The excessive use may affect the phytoplankton and have negative consequences in the aquatic ecosystem.
Topics: Insecticides; Chlorpyrifos; Dimethoate; Ecosystem; Hydrogen Peroxide; Oxidative Stress; Pesticides; Antioxidants; Chlorophyll; Chlorophyll A; Organophosphorus Compounds
PubMed: 37729300
DOI: 10.1590/0001-3765202320200463 -
BMC Pharmacology & Toxicology Jan 2024Multiple pesticides are often used in combination for plant protection and public health. Therefore, it is important to analyze the physiological changes induced by...
BACKGROUND
Multiple pesticides are often used in combination for plant protection and public health. Therefore, it is important to analyze the physiological changes induced by multiple pesticides exposure. The objective of this study was to investigate the combined toxicity of the widely-used organophosphorus and pyrethroid pesticides diazinon, dimethoate, and cypermethrin.
METHODS
Male Wistar rats were administrated by gavage once daily with the three pesticides individual or in combination for consecutive 28 days. The metabolic components of serum and urine samples were detected by using H nuclear magnetic resonance (NMR)-based metabolomics method. Histopathological examination of liver and kidneys and serum biochemical determination were also carried out.
RESULTS
The results showed that after the 28-day subacute exposure, serum glutamic transaminase and albumin were significantly increased and blood urea nitrogen was significantly decreased in the rats exposed to the mixture of the pesticides compared with the control rats, suggesting that the co-exposure impaired liver and kidney function. Metabolomics analysis indicated that the indicators 14 metabolites were statistically significant altered in the rats after the exposure of the pesticides. The increase in 3-hydroxybutyric acid in urine or decrease of lactate and N-acetyl-L-cysteine in serum could be a potentially sensitive biomarker of the subchronic combined effects of the three insecticides. The reduction level of 2-oxoglutarate and creatinine in urine may be indicative of dysfunction of liver and kidneys.
CONCLUSION
In summary, the exposure of rats to pesticides diazinon, dimethoate, and cypermethrin could cause disorder of lipid and amino acid metabolism, induction of oxidative stress, and dysfunction of liver and kidneys, which contributes to the understanding of combined toxic effects of the pesticides revealed by using the metabolomics analysis of the urine and serum profiles.
Topics: Rats; Animals; Diazinon; Dimethoate; Rats, Wistar; Pyrethrins; Pesticides; Liver
PubMed: 38167230
DOI: 10.1186/s40360-023-00714-6 -
Open Veterinary Journal Jul 2023Dimethoate (DM) is one of the most important organophosphate insecticides used for controlling many pests which affect vegetables, fruits, and agricultural crops, its...
BACKGROUND
Dimethoate (DM) is one of the most important organophosphate insecticides used for controlling many pests which affect vegetables, fruits, and agricultural crops, its persistence in soils and crops could cause a health hazard to humans as well as other non-target organisms.
AIM
This study was conducted to evaluate the effect of the recommended dose and its double of DM on sex hormones, sperm morphology, and fertility of adult male mice.
METHODS
Twenty-seven Swiss albino adult male mice were divided into three groups of nine animals each: control group received distilled water only, while other groups received DM orally at doses (0.1 and 0.2 ml DM/100 ml distilled water) for 20 days, at the end of the treatment, six mice from each group were sacrificed. The sperm morphology was evaluated and sex hormones were measured. Three mice from each group were allowed to mate with untreated females (1:2).
RESULT
The results revealed a decrease in luteinizing hormone levels in mice treated with (0.2 ml DM/100 ml distilled water) compared with the control group while the levels of follicle-stimulating hormone and testosterone did not record any significant differences. Also, the results demonstrated a significant increase in abnormal sperm morphology such as head and tail. The fertility was reduced and the average number of dead embryos increased while the average number of live embryos decreased.
CONCLUSION
This current study confirmed that DM has detrimental effects on sperm morphology, fertility, and the embryos; therefore, more efforts should be exerted to protect ourselves and our environment from the harmful effects of this pesticide.
Topics: Female; Humans; Male; Animals; Mice; Dimethoate; Semen; Infertility; Spermatozoa; Water
PubMed: 37614725
DOI: 10.5455/OVJ.2023.v13.i7.9 -
Toxicology Reports Dec 2023This paper reviewed the published data on the levels of different pesticide residues in vegetables (tomato, eggplant, beans, gourds, cauliflower, cabbage, cucumber,... (Review)
Review
This paper reviewed the published data on the levels of different pesticide residues in vegetables (tomato, eggplant, beans, gourds, cauliflower, cabbage, cucumber, potato, carrot, onion, red chilli, red amaranth, lady's finger, spinach, coriander, and lettuce) from Bangladesh in the last decade. Vegetable production in Bangladesh has increased tremendously (37.63%) compared to the last decades, along with its pesticide use. The most observed pesticide groups used in vegetable production were organophosphorus, pyrethroids, carbamate, organochlorine, nereistoxin analogue group, and neonicotinoids. More specifically, chlorpyrifos, dimethoate, diazinon, and malathion were the most used pesticides. More than 29% of the vegetable samples (1577) were contaminated with pesticide residue; among the contaminated samples (458), most cases (73%) exceeded the maximum residue limits (MRLs). The pesticide-contaminated vegetables were cucumber (51%), tomato (41%), cauliflower (31%), miscellaneous vegetables (36%), eggplant (29%), beans (23%), cabbage (18%), and gourds (16%). Among the pesticide-contaminated samples, vegetables with above MRL were gourds (100%), beans (92), tomato (78%), eggplant (73%), miscellaneous vegetables (69%), cucumber (62%), cabbage (50%), cauliflower (50%) (p < 0.05). It was also observed that a single vegetable was often contaminated with multiple pesticides, and farmers did not follow a proper withdrawal period while using pesticides. Hazard quotation (HQ>1) was observed in adolescents and adults in tomato, eggplant, beans, cauliflower, cabbage, cucumber, lady's finger, lettuce, and coriander. There was no health risk observed (HQ<1) in gourds, potato, carrot, onion, red chilli, red amaranth, spinach, and okra. The highest acute and chronic HQ (aHQ, cHQ) was observed for cypermethrin (bean) in adolescents (aHQ=255, cHQ= 510) and adults (aHQ=131, cHQ=263). It was also observed that these pesticides harmed air, soil, water, and non-target organisms. Nevertheless, the review will help the government develop policies that reduce pesticide use and raise people's awareness of its harmful effects.
PubMed: 37711360
DOI: 10.1016/j.toxrep.2023.09.003 -
Chemosphere Jan 2024The worldwide and intensive use of phytosanitary compounds results in environmental and food contamination by chemical residues. Human exposure to multiple pesticide...
Low concentrations of ethylene bisdithiocarbamate pesticides maneb and mancozeb impair manganese and zinc homeostasis to induce oxidative stress and caspase-dependent apoptosis in human hepatocytes.
The worldwide and intensive use of phytosanitary compounds results in environmental and food contamination by chemical residues. Human exposure to multiple pesticide residues is a major health issue. Considering that the liver is not only the main organ for metabolizing pesticides but also a major target of toxicities induced by xenobiotics, we studied the effects of a mixture of 7 pesticides (chlorpyrifos-ethyl, dimethoate, diazinon, iprodione, imazalil, maneb, mancozeb) often detected in food samples. Effects of the mixture was investigated using metabolically competent HepaRG cells and human hepatocytes in primary culture. We report the strong cytotoxicity of the pesticide mixture towards hepatocytes-like HepaRG cells and human hepatocytes upon acute and chronic exposures at low concentrations extrapolated from the Acceptable Daily Intake (ADI) of each compound. Unexpectedly, we demonstrated that the manganese (Mn)-containing dithiocarbamates (DTCs) maneb and mancozeb were solely responsible for the cytotoxicity induced by the mixture. The mechanism of cell death involved the induction of oxidative stress, which led to cell death by intrinsic apoptosis involving caspases 3 and 9. Importantly, this cytotoxic effect was found only in cells metabolizing these pesticides. Herein, we unveil a novel mechanism of toxicity of the Mn-containing DTCs maneb and mancozeb through their metabolization in hepatocytes generating the main metabolite ethylene thiourea (ETU) and the release of Mn leading to intracellular Mn overload and depletion in zinc (Zn). Alteration of the Mn and Zn homeostasis provokes the oxidative stress and the induction of apoptosis, which can be prevented by Zn supplementation. Our data demonstrate the hepatotoxicity of Mn-containing fungicides at very low doses and unveil their adverse effect in disrupting Mn and Zn homeostasis and triggering oxidative stress in human hepatocytes.
Topics: Humans; Maneb; Manganese; Pesticides; Zineb; Fungicides, Industrial; Apoptosis; Oxidative Stress; Zinc; Hepatocytes; Ethylenes; Homeostasis
PubMed: 37923018
DOI: 10.1016/j.chemosphere.2023.140535 -
Biosensors Jan 2024Dimethoate contaminants in food pose a threat to human health. Rapid and sensitive trace detection methods are required to keep food safe. In this study, a novel...
Dimethoate contaminants in food pose a threat to human health. Rapid and sensitive trace detection methods are required to keep food safe. In this study, a novel fluorescent aptasensor was developed for the sensitive detection of dimethoate based on carbon quantum dots labeled with double-stranded DNA (CQDs-apt-cDNA) and TiCT flakes. Under optimal conditions, the aptasensor showed a good linear range of 1 × 10 to 5 × 10 M for dimethoate with a coefficient of determination (R) of 0.996. Besides, a low detection limit of 2.18 × 10 M was obtained. The aptasensor showed high selectivity in interference samples and good reproducibility with an RSD of 3.06% (<5%) for dimethoate detection. Furthermore, the proposed aptasensor was applied to the detection of dimethoate in apple juice and tap water with satisfactory recoveries from 96.2 to 104.4%. Because of these benefits, this aptasensor has the potential and promise for detecting food contaminants in the food industry.
Topics: Humans; Dimethoate; Pesticides; Reproducibility of Results; Titanium; Limit of Detection; Aptamers, Nucleotide; Biosensing Techniques; Nitrites; Transition Elements
PubMed: 38391988
DOI: 10.3390/bios14020069 -
Environmental Analysis, Health and... Mar 2024For farmers around the world to protect crops from disturbing pests, it is common to use pesticides to ward off the growth of pests or even eliminate them. Even though...
For farmers around the world to protect crops from disturbing pests, it is common to use pesticides to ward off the growth of pests or even eliminate them. Even though pesticides are seen as a good thing for protecting crops, there is one thing that mustn't be forgotten the origin of the pesticide itself is a toxin compound that is dangerous if used irresponsibly. The main concern of this study is excessive use of pesticides may cause serious consequences to the ecosystem and environment through the accumulation of pesticide residue by irresponsible farmers. To minimize the effects of pesticide residues, the selection of the type of pesticide needs to be considered which type may not be harmful to the environment's health even though accumulation happens. Therefore, in this study, a fuzzy-based computational model assessor was built to measure the safety level of pesticides toward the environment. The fuzzy model was created with consideration of several parameters related to pesticide behaviors, its effects on beneficial organisms, and its persistence in the environment. The method used for this study includes literature reviewing, fuzzification, statistical approach, expert knowledge sharing, and quantitative analysis. The model created in this study can assist in a more accurate and realistic method of selecting better pesticide options that will be used by farmers. To ensure the validity of the model, verifying and validating the formula and pesticide result assessment were done with related literature articles. In this study, from 10 types of pesticides used as a sample, dodine, and iprodione pesticides are the best option for protecting crops with a safety level of 7.36, and abamectin, dimethoate, chorpyrifos, and methidathion are not safe options for farming use because of its potential of harming the environment.
PubMed: 38631395
DOI: 10.5620/eaht.2024003 -
Scientific Reports Aug 2023Pollinators in agricultural landscapes are facing global decline and the main pressures include food scarcity and pesticide usage. Intensive agricultural landscapes may...
Pollinators in agricultural landscapes are facing global decline and the main pressures include food scarcity and pesticide usage. Intensive agricultural landscapes may provide important food resources for wild pollinators via mass flowering crops. However, these are monofloral, short-term, and may contain pesticide residues. We explored how the landscape composition with a different proportion of oilseed rape (6-65%) around Osmia bicornis nests affects floral diversity, contamination with pesticides, and energetic value of provisions collected by this species of wild bees as food for their offspring. Altogether, the bees collected pollen from 28 plant taxa (6-15 per nest) and provisions were dominated by Brassica napus (6.0-54.2%, median 44.4%, 12 nests), Quercus sp. (1.2-19.4%, median 5.2%, 12 nests), Ranunculus sp. (0.4-42.7%, median 4.7%, 12 nests), Poaceae (1.2-59.9%, median 5.8%, 11 nests) and Acer sp. (0.6-42%, median 18.0%, 8 nests). Residues of 12 pesticides were found in provisions, with acetamiprid, azoxystrobin, boscalid, and dimethoate being the most frequently detected at concentrations up to 1.2, 198.4, 16.9 and 17.8 ng/g (median 0.3, 10.6, 11.3, 4.4 ng/g), respectively. Floral diversity and energetic value of provisions, but not the Pesticide Risk Index depended on landscape structure. Moreover, pollen diversity decreased, and energetic value increased with landscape diversity. Thus, even a structurally simple landscape may provide diverse food for O. bicornis if the nest is located close to a single but resource-diverse patch. Both B. napus and non-crop pollen were correlated with pesticide concentrations.
Topics: Bees; Animals; Pesticide Residues; Brassica napus; Pesticides; Acer; Crops, Agricultural
PubMed: 37591888
DOI: 10.1038/s41598-023-39950-5 -
Scientific Reports Jun 2023Agrichemicals such as organophosphorus pesticides' metabolites (OPPMs) are more hazardous and pervasive than their parent pesticides. Parental germline exposure to such...
Agrichemicals such as organophosphorus pesticides' metabolites (OPPMs) are more hazardous and pervasive than their parent pesticides. Parental germline exposure to such xenobiotics leads to an elevated susceptibility towards reproductive failures e.g. sub- or in-fertility. This study sought to examine the effects of low-dose, acute OPPM exposure on mammalian sperm function using buffalo as the model organism. The buffalo spermatozoa were briefly (2 h) exposed to metabolites of the three most prevalent organophosphorus pesticides (OPPs) viz. Omethoate (from Dimethoate), paraoxon-methyl (from methyl/ethyl parathion) and 3, 5, 6-trichloro-2-pyridinol (from chlorpyrifos). Exposure to OPPMs resulted in compromised structural and functional integrity (dose-dependent) of the buffalo spermatozoa typified by elevated membrane damage, increased lipid peroxidation, precocious capacitation and tyrosine phosphorylation, perturbed mitochondrial activity and function and (P < 0.05). This led to a decline in the in vitro fertilizing ability (P < 0.01) of the exposed spermatozoa, as indicated by reduced cleavage and blastocyst formation rates. Preliminary data indicate that acute exposure to OPPMs, akin to their parent pesticides, induces biomolecular and physiological changes in spermatozoa that compromise their health and function ultimately affecting their fertility. This is the first study demonstrating the in vitro spermatotoxic effects of multiple OPPMs on male gamete functional integrity.
Topics: Animals; Male; Buffaloes; Fertility; Methyl Parathion; Organophosphorus Compounds; Pesticides; Semen; Sperm Motility; Spermatozoa
PubMed: 37277402
DOI: 10.1038/s41598-023-35541-6