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International Journal of Sports... 2023Normative data is useful for comparing measured values of strength with population norms and can avoid the issues associated with limb symmetry index. The available...
BACKGROUND
Normative data is useful for comparing measured values of strength with population norms and can avoid the issues associated with limb symmetry index. The available normative shoulder strength values are limited by constraints on research designs and variability in subject groups which prevents this data being successfully extrapolated to the greater population.
PURPOSE
The purpose of this study was to establish normative isometric strength values for various movements of the shoulder that are specific to function and rotator cuff strength. A secondary goal of this study was to analyze the effect of age, gender, weight, height, activity level and arm dominance on shoulder strength.
DESIGN
Observational cohort study.
METHODS
Subjects in four age groups (20-29, 30-39, 40-49, 50-59) were included in this study-200 males (40.0 ± 11.6 years, 179.1 ± 6.5 cm, 81 ± 13.0 kg) and 200 females (40.1 ± 11.5 years, 165.3 ± 7.4sm, 64.4 ± 11.6 kg). Bilateral isometric strength measurements were taken with a handheld dynamometer testing seven shoulder movements. Tables of normative strength data were constructed. Multivariate analyses were performed to analyze the effects of age, gender, weight, height and activity level on isometric shoulder strength.
RESULTS
Men were stronger than women (p<0.001). Age was not associated with most strength measures with the exception of dominant arm abduction (p<0.004), non-dominant arm abduction (p<0.028) and non-dominant arm scapular plane abduction (p<0.004) which had a negative association with strength. Weight was positively associated with strength (p<0.001). Activity level was positively associated with all strength measures (p<0.05) except dominant sided abduction (p=0.056). There were no statistically significant differences between dominant and non-dominant sides.
CONCLUSION
This normative data may be useful to the clinician, as it permits a standard against which to compare shoulder strength for various age groups. Clinicians can have confidence that the uninvolved limb, if symptom free, can be used as an adequate benchmark for strength measures.
LEVELS OF EVIDENCE
Level 3©The Author(s).
PubMed: 37547848
DOI: 10.26603/001c.83938 -
International Journal of Women's Health 2023To explore the correlation of female vaginal microbiota and immune factors with cervical cancer.
OBJECTIVE
To explore the correlation of female vaginal microbiota and immune factors with cervical cancer.
METHODS
The distribution pattern difference of vaginal microbiota of four groups of women (cervical cancer, HPV-positive CIN, HPV-positive non-CIN, and HPV-negative groups) were compared by microbial 16S rDNA sequencing. The protein chip was used to detect the composition and changes of the immune factors in the four groups.
RESULTS
Alpha diversity analysis demonstrated that the diversity of the vaginal microbiota was increased as the disease develops. Among those bacteria abundant in the vaginal microbiota, , and dominate at the genus level of vaginal flora. Compared with the HPV-negative group, the differentially dominant bacteria, such as and , are enriched in the cervical cancer group. Likewise, , and are more in the HPV-positive CIN group, while and in the HPV-positive non-CIN group, respectively. In contrast, and are dominant in the HPV-negative group (LDA>4log10). The concentration of inflammatory immune factors IP-10 and VEGF-A were increased in the cervical cancer group ( < 0.05), compared with other groups.
CONCLUSION
The occurrence of cervical cancer is related to an increase of vaginal microbiota diversity and up-regulation of inflammatory immune factor proteins. The abundance of was decreased while the one of and were increased in the cervical cancer group, compared with other three groups. Moreover, the IP-10 and VEGF-A were also increased in the cervical cancer group. Thus, evaluation of changes in the vaginal microbiota and these two immune factor levels might be a potential non-invasive and simple method to predict cervical cancer. Furthermore, it is significant to adjust and restore the balance of vaginal microbiota and maintain normal immune function in preventing and treating cervical cancer.
PubMed: 37424699
DOI: 10.2147/IJWH.S406596 -
Chemosphere Jul 2023Electronic waste is a source of both legacy and emerging flame retardants to the environment, especially in regions where sufficient waste handling systems are lacking....
Electronic waste is a source of both legacy and emerging flame retardants to the environment, especially in regions where sufficient waste handling systems are lacking. In the present study, we quantified the occurrence of short- and medium chain chlorinated paraffins (SCCPs and MCCPs) and dechloranes in household chicken (Gallus domesticus) eggs and soil collected near waste disposal sites on Zanzibar and the Tanzanian mainland. Sampling locations included an e-waste facility and the active dumpsite of Dar es Salaam, a historical dumpsite in Dar es Salaam, and an informal dumpsite on Zanzibar. We compared concentrations and contaminant profiles between soil and eggs, as free-range chickens ingest a considerable amount of soil during foraging, with potential for maternal transfer to the eggs. We found no correlation between soil and egg concentrations or patterns of dechloranes or CPs. CPs with shorter chain lengths and higher chlorination degree were associated with soil, while longer chain lengths and lower chlorination degree were associated with eggs. MCCPs dominated the CP profile in eggs, with median concentrations ranging from 500 to 900 ng/g lipid weight (lw) among locations. SCCP concentrations in eggs ranged from below the detection limit (LOD) to 370 ng/g lw. Dechlorane Plus was the dominating dechlorane compound in all egg samples, with median concentrations ranging from 0.5 to 2.8 ng/g lw. SCCPs dominated in the soil samples (400-21300 ng/g soil organic matter, SOM), except at the official dumpsite where MCCPs were highest (65000 ng/g SOM). Concentrations of dechloranes in soil ranged from below LOD to 240 ng/g SOM, and the dominating compounds were Dechlorane Plus and Dechlorane 603. Risk assessment of CP levels gave margins of exposure (MOE) close to or below 1000 for SCCPs at one location.
Topics: Animals; Chickens; Tanzania; Paraffin; Soil; Environmental Monitoring; Hydrocarbons, Chlorinated; Waste Disposal Facilities; China
PubMed: 37037350
DOI: 10.1016/j.chemosphere.2023.138646 -
Trends in Plant Science Sep 2023Subtropical and tropical forests in Asia often comprise canopy dominant trees that form symbioses with ectomycorrhizal fungi, and species-rich understorey trees that... (Review)
Review
Subtropical and tropical forests in Asia often comprise canopy dominant trees that form symbioses with ectomycorrhizal fungi, and species-rich understorey trees that form symbioses with arbuscular mycorrhizal fungi. We propose a virtuous phosphorus acquisition hypothesis to explain this distinct structure. The hypothesis is based on (i) seedlings being rapidly colonised by ectomycorrhizal fungi from established mycelial networks that generates positive feedback and resistance to pathogens, (ii) ectomycorrhizal fungi having evolved a suite of morphological, physiological, and molecular traits to enable them to capture phosphorus from a diversity of chemical forms, including organic forms, and (iii) allocation of photosynthate carbon from adult host plants to provide the energy needed to undertake these processes.
Topics: Forests; Symbiosis; Mycorrhizae; Trees; Phosphorus; Soil Microbiology; Soil
PubMed: 37087357
DOI: 10.1016/j.tplants.2023.03.027 -
Frontiers in Cellular and Infection... 2024The present treatments for bronchiectasis, which is defined by pathological dilatation of the airways, are confined to symptom relief and minimizing exacerbations. The... (Review)
Review
The present treatments for bronchiectasis, which is defined by pathological dilatation of the airways, are confined to symptom relief and minimizing exacerbations. The condition is becoming more common worldwide. Since the disease's pathophysiology is not entirely well understood, developing novel treatments is critically important. The interplay of chronic infection, inflammation, and compromised mucociliary clearance, which results in structural alterations and the emergence of new infection, is most likely responsible for the progression of bronchiectasis. Other than treating bronchiectasis caused by cystic fibrosis, there are no approved treatments. Understanding the involvement of the microbiome in this disease is crucial, the microbiome is defined as the collective genetic material of all bacteria in an environment. In clinical practice, bacteria in the lungs have been studied using cultures; however, in recent years, researchers use next-generation sequencing methods, such as 16S rRNA sequencing. Although the microbiome in bronchiectasis has not been entirely investigated, what is known about it suggests that , and dominate the lung bacterial ecosystems, they present significant intraindividual stability and interindividual heterogeneity. and -dominated microbiomes have been linked to more severe diseases and frequent exacerbations, however additional research is required to fully comprehend the role of microbiome in the evolution of bronchiectasis. This review discusses recent findings on the lung microbiota and its association with bronchiectasis.
Topics: Bronchiectasis; Humans; Microbiota; Lung; Bacteria; RNA, Ribosomal, 16S
PubMed: 38895737
DOI: 10.3389/fcimb.2024.1405399 -
JBRA Assisted Reproduction Sep 2023The vaginal microbiome is dominated by Lactobacillus spp. and the depletion of these microorganisms have been associated with adverse conditions that can affect women's... (Review)
Review
The vaginal microbiome is dominated by Lactobacillus spp. and the depletion of these microorganisms have been associated with adverse conditions that can affect women's health. Disturbance of the vaginal niche with a non-lactobacillary microbiota is associated with susceptibility to some diseases, such as obstetric alterations and infertility, resulting in failure in natural pregnancies and increased demand for assisted reproduction treatments. The present study sought to understand the influence of Lactobacillus spp. and fertility female. A systematic search was performed in the following databases: PubMed, MEDLINE, SciELO and LILACS, using the keywords: "Microbiome"; "Lactobacillus" and "Female Infertility", published in the last five years. The search resulted in 92 articles; however, 38 articles were excluded due to duplicity, 23 articles were excluded in the selection title/abstract, leaving 31 articles for full reading. In the end, 18 articles were analyzed. The studies encompassed a total of 2,011 women, using 27 types of samples to verify the composition of the microbiome. The eighteen articles that reported the microbiome of fertile women were constituted by a dominance of Lactobacillus spp. who joined to positive predictive outcomes in reproduction, while infertile women showed a dysbiotic profile. Therefore, analyzing bacterial patterns would allow a personalized diagnosis, which could favor personalized therapy for prevention and treatment of certain diseases.
Topics: Pregnancy; Female; Humans; Lactobacillus; Infertility, Female; Vagina; Reproduction; Fertility
PubMed: 37417853
DOI: 10.5935/1518-0557.20230006 -
Lupus Science & Medicine Jul 2023Since molecularly targeted therapies are emerging for treating lupus nephritis (LN), this study aimed to assess the immunohistochemical findings of the cytokines in...
Differential expression of IFN-α, IL-12 and BAFF on renal immune cells and its relevance to disease activity and treatment responsiveness in patients with proliferative lupus nephritis.
OBJECTIVE
Since molecularly targeted therapies are emerging for treating lupus nephritis (LN), this study aimed to assess the immunohistochemical findings of the cytokines in renal tissue and their pathological and clinical relevance in LN.
METHODS
Fifty patients with proliferative LN formed the case group; 5 with LN class II, IgA nephropathy and 10 with idiopathic haematuria were enrolled as controls. Immunohistochemical analysis for CD3, CD20, interferon (IFN)-α, interleukin (IL)-12/p40 and B-cell activating factor (BAFF) was performed by scoring the number of positive cells/area of the cortex. All immunohistochemical investigations were performed on formalin-fixed paraffin-embedded renal tissue. Proliferative LN cases were grouped by the dominant expression of IFN-α, IL-12/p40 and BAFF, and subsequently, clinicopathological features were compared.
RESULTS
Clinical data of patients with proliferative LN included urine protein creatinine ratio, 2.2 g/gCre; anti-double-stranded DNA antibody, 200.9 IU/mL; total complement activity (CH50), 21.9 U/mL and SLE Disease Activity Index, 19.8 points. Proliferative LN cases, including class III (n=18) and IV (n=32), were classified into three subgroups according to the immunohistochemical score based on the dominancy of IFN-α (n=17), IL-12 (n=16) and BAFF group (n=17) proteins. Hypocomplementaemia and glomerular endocapillary hypercellularity were significantly increased in the IFN-α group, whereas chronic lesions were significantly higher in the IL-12 group (p<0.05). The IFN-α group had a poorer renal prognosis in treatment response after 52 weeks.
CONCLUSIONS
The immunohistochemistry (IHC) of IFN-α, IL-12 and BAFF for proliferative LN enabled grouping. Especially, the IFN-α and IL-12 groups showed different clinicopathological features and renal prognoses. The results indicated the possibility of stratifying cases according to the IHC of target molecules, which might lead to precision medicine.
Topics: Humans; Lupus Nephritis; Interleukin-12; B-Cell Activating Factor; Lupus Erythematosus, Systemic; Kidney
PubMed: 37460249
DOI: 10.1136/lupus-2023-000962 -
Medical Education Online Dec 2024The negative impact of medical training on trainee mental health continues to be a concern. Situated within a sociocultural milieu, Generation Z and Generation Y,...
INTRODUCTION
The negative impact of medical training on trainee mental health continues to be a concern. Situated within a sociocultural milieu, Generation Z and Generation Y, defined by their highly involved parents and the widespread use of technology, currently dominate undergraduate and graduate medical education respectively. It is necessary to explore medical trainees' generational characteristics and job-related factors related to stress, burnout, depression, and resilience. This might provide different perspectives and potential solutions to medical trainees' mental health.
METHODS
A cross-sectional study was conducted among medical trainees (students and residents) from two institutions in Qatar. A self-administered online survey included measures for trainees' social media overuse, their parent's parenting style, the educational support by the clinical teacher, job (demands, control, and support), and work-life balance and their relation with their stress, burnout, depression, and resilience. Relationships were tested with multiple linear regression analyses.
RESULTS
Of the 326 medical trainees who responded, 142 (44%) trainees - 93 students and 49 residents - completed all items and were included in the analysis. Social media overuse and inability to maintain a work-life balance were associated with higher levels of stress, depression, and student burnout. Higher levels of job support were associated with lower levels of stress, depression, and resident burnout, and a higher level of resilience. Job control was associated with lower burnout levels. Parenting style was unrelated to trainees' mental health.
DISCUSSION
The two generations 'Y' and 'Z' dominating current medical training showed more stress-related complaints when there is evidence of social media overuse and failure to maintain a work-life balance, while job support counterbalances this, whereas parenting style showed no effect. Measures to enhance medical trainees' mental health may include education about the wise use of social media, encouraging spending more quality social time, and enhancing job support and job control.
Topics: Humans; Mental Health; Cross-Sectional Studies; Burnout, Professional; Education, Medical, Graduate; Resilience, Psychological; Surveys and Questionnaires
PubMed: 38488138
DOI: 10.1080/10872981.2024.2329404 -
Journal of Eye Movement Research 2023The aim of the study was to analyze the stability of dominant and non-dominant eye fixations, as well as the influence of development on fixation stability. The study...
The aim of the study was to analyze the stability of dominant and non-dominant eye fixations, as well as the influence of development on fixation stability. The study analyzed fixation stability in 280 school-age children, ranging in age from 7 to 12 years old. Fixation stability was determined by calculating the bivariate contour ellipse area (BCEA). During the fixation task, eye movements were recorded using the Tobii Pro Fusion eye tracking device at a 250 Hz sampling frequency. The results indicate that the fixation stability of dominant and non-dominant eyes, as well as the fixation stability of each eye regardless of dominance, improves as children grow older. It was found that for 7 and 8- year-old children, fixation in the dominant eye is significantly more stable than in the non-dominant eye, while in older children, there is no significant difference in fixation stability between the dominant and non-dominant eye.
PubMed: 38370528
DOI: 10.16910/jemr.16.3.6 -
Frontiers in Neurology 2023Myelin oligodendrocyte glycoprotein (MOG) is expressed on the outermost layer of the myelin sheath in the central nervous system. Recently, the clinical concept of MOG... (Review)
Review
Pathology of myelin oligodendrocyte glycoprotein antibody-associated disease: a comparison with multiple sclerosis and aquaporin 4 antibody-positive neuromyelitis optica spectrum disorders.
Myelin oligodendrocyte glycoprotein (MOG) is expressed on the outermost layer of the myelin sheath in the central nervous system. Recently, the clinical concept of MOG antibody-associated disease (MOGAD) was established based on the results of human MOG-transfected cell-based assays which can detect conformation-sensitive antibodies against MOG. In this review, we summarized the pathological findings of MOGAD and discussed the issues that remain unresolved. MOGAD pathology is principally inflammatory demyelination without astrocyte destruction, characterized by perivenous demyelination previously reported in acute disseminated encephalomyelitis and by its fusion pattern localized in both the white and gray matter, but not by radially expanding confluent demyelination typically seen in multiple sclerosis (MS). Some of demyelinating lesions in MOGAD show severe loss of MOG staining compared with those of other myelin proteins, suggesting a MOG-targeted pathology in the disease. Perivascular cuffings mainly consist of macrophages and T cells with CD4-dominancy, which is also different from CD8+ T-cell-dominant inflammation in MS. Compared to aquaporin 4 (AQP4) antibody-positive neuromyelitis optica spectrum disorders (NMOSD), perivenous complement deposition is less common, but can be seen on myelinated fibers and on myelin degradation products within macrophages, resembling MS Pattern II pathology. Thus, the pathogenetic contribution of complements in MOGAD is still debatable. Together, these pathological features in MOGAD are clearly different from those of MS and AQP4 antibody-positive NMOSD, suggesting that MOGAD is an independent autoimmune demyelinating disease entity. Further research is needed to clarify the exact pathomechanisms of demyelination and how the pathophysiology relates to the clinical phenotype and symptoms leading to disability in MOGAD patients.
PubMed: 37545724
DOI: 10.3389/fneur.2023.1209749