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Journal of Advanced Veterinary and... Sep 2023This research aimed to assess the prevalence of caprine pasteurellosis, isolate and identify pasteurellosis ( and ) in pneumonic goats, and discover the main bacterial... (Review)
Review
OBJECTIVE
This research aimed to assess the prevalence of caprine pasteurellosis, isolate and identify pasteurellosis ( and ) in pneumonic goats, and discover the main bacterial cause of pneumonia.
MATERIALS AND METHODS
One hundred and five samples (94 nasal swabs and 11 lung tissues) from goats suspected of having pneumonia were taken and transferred aseptically to the laboratory. Following the processing of the collected samples, spp. was isolated with the aid of plate culture methods. Biochemical characteristics were used to identify all bacterial isolates, which were then verified by polymerase chain reaction (PCR). Antimicrobial susceptibility testing was also carried out to evaluate the sensitivity profiles of various antibiotics. The serotype-specific antigen (PHSSA) gene was used to identify isolates of , and the KMT1 gene was used to identify isolates of .
RESULTS
From the 105 clinically suspicious samples, 51 (48.57%) were identified to be through bacteriological testing and also by PCR targeting the gene. Of these, 47.87% (45/94) were nasal swabs, and 54.55% (6/11) were lung tissues. Among confirmed samples, 70.59% (36/51) were identified as and 29.41% (15/51) were identified as . Resistance to tetracycline, streptomycin, oxytetracycline, gentamicin, and ceftriaxone was found in 50%-83% of the isolates. In addition, PCR identified the PHSSA and KMT1 genes from isolates of and , respectively.
CONCLUSION
The present study revealed that and primarily caused pasteurellosis in pneumonic goats in Bangladesh. However, when treating these animals, the proper choice of antimicrobials should be made to control this disease.
PubMed: 37969786
DOI: 10.5455/javar.2023.j707 -
Veterinary Medicine (Auckland, N.Z.) 2023Pneumonic pasteurellosis is a respiratory system disease of sheep caused by , and responsible for the low productivity and economic loss resulting from death and...
Isolation and Molecular Detection of and from Clinically Pneumonic Pasteurellosis Cases of Bonga Sheep Breed and Their Antibiotic Susceptibility Tests in Selected Areas of Southwest Ethiopian Peoples Regional State, Ethiopia.
BACKGROUND
Pneumonic pasteurellosis is a respiratory system disease of sheep caused by , and responsible for the low productivity and economic loss resulting from death and treatment costs. This study was conducted to isolate and molecularly detect causative agents and antibiotic susceptibility tests from a nasal swab sample of the Bonga sheep breed that was suspected to have pneumonic pasteurellosis in selected areas of Southwest Ethiopian Peoples Regional State.
METHODS
A cross-sectional study design was used along with purposive sampling of nasal swab samples from sheep that were brought to veterinary clinics during the study period. Bacterial isolation and phenotypic characterization were carried out using microbiological and biochemical tests that followed standard microbiological techniques. To molecularly confirm the isolates, and , species-specific PCR primer genes were used. Using the disc diffusion method, molecularly confirmed isolates were subjected to an in vitro antibiotic susceptibility test.
RESULTS
The 85 samples that were scrutinized had an overall isolation rate of 31.76%, whereas the isolates of and had species compositions of 40.7% and 59.25%, respectively. Overall, 12.5% of the and 18.18% of the species were verified from phenotypical isolates using the species-specific PCR primer genes and , respectively. An in vitro antibiotic susceptibility test was carried out on all four PCR-confirmed isolates for seven commonly used antibiotics used to treat ovine pasteurellosis in the study area. It was found that both bacterial species were resistant to chloramphenicol and penicillin G.
CONCLUSION
Using phenotypic and molecular diagnostic techniques, the results of our current inquiry revealed that and are the causative agents of ovine pneumonic pasteurellosis in the study area.
PubMed: 38162823
DOI: 10.2147/VMRR.S435932 -
Veterinary Medicine and Science Jul 2023Small ruminants are the most numerous of man's domestic livestock. Although sheep represent a great resource for Ethiopia, the net rate of productivity per animal is...
Isolation and identification of Mannheimia haemolytica and Pasteurella multocida from symptomatic and asymptomatic sheep and their antibiotic susceptibility patterns in three selected districts of north Gondar zone, Gondar Ethiopia.
BACKGROUND
Small ruminants are the most numerous of man's domestic livestock. Although sheep represent a great resource for Ethiopia, the net rate of productivity per animal is very low due to many factors including respiratory disorders.
OBJECTIVES
The objectives of this work were to isolate and identify M. haemolytica and P. multocida as well as to assess the antibiotic susceptibility patterns of these isolates. Nasal swab samples were collected aseptically by using 70% alcohol as a disinfectant.
METHODS
A cross-sectional study was conducted in three selected districts of the north Gondar zone, Ethiopia.
RESULTS
From 148 samples collected in 94 (63.5%) asymptomatic and 54 (35.5%) symptomatic sheep, a total of 23 were isolated successfully based on cultural, staining, and biochemical characteristics. Of these isolates, 18 (78.3%) and 5 (21.7%) were M. haeimolytica and P. multocida, respectively. Compared with the total animals examined, the proportion of M. haeimolytica and P. multocida were 12.16 % (n = 18) and 3.38% (n = 5), respectively. All of the isolates were subjected to a panel of 8 antibiotic discs for sensitivity testing. Of the tested antibiotics, chloramphenicol (100%), gentamicin, and tetracycline (82.6%) each and co-trimoxazole (60.8%) were found to be the most effective drugs whereas, both species were completely resistant to vancomycin and showed a very low degree of susceptibility for the rest drugs.
CONCLUSIONS
In conclusion, M. haemolytica was found to be the predominant isolate in all host-related factors and most of the antibiotics were not fully effective against the isolates. Hence, treatment and/or vaccination of ovine pneumonic pasteurellosis should be emphasised to M. haeimolytica using the most effective drugs along with appropriate herd management practices.
Topics: Sheep; Animals; Mannheimia haemolytica; Pasteurella multocida; Ethiopia; Cross-Sectional Studies; Anti-Bacterial Agents
PubMed: 37197762
DOI: 10.1002/vms3.1166 -
Veterinary Pathology Mar 2024-associated abomasitis has been clinically described as a cause of sudden death in lambs, but it is poorly characterized. We describe the pathological features of a...
-associated abomasitis has been clinically described as a cause of sudden death in lambs, but it is poorly characterized. We describe the pathological features of a severe fibrinonecrotizing abomasitis in 3 lambs that died suddenly. All 3 abomasums had a thickened submucosa due to edema and necrotic areas delimited by bands of degenerate neutrophils with slender nuclei (oat cells) and angiocentric distributions. The overlying mucosa was congested. Myriads of gram-negative coccobacilli were observed within the oat cell bands. was isolated from the abomasum in all 3 animals and was serotyped as A2 in one of them. Pericarditis and pleuritis were observed in 2 of the lambs. spp. were isolated in 1 lamb and detected by immunohistochemistry in the 3 animals, suggesting clostridial co-infection. should be considered among the differential diagnoses of necrotizing abomasitis in lambs.
PubMed: 38440930
DOI: 10.1177/03009858241235393 -
TheScientificWorldJournal 2024species are frequently encountered as serious diseases in small ruminants. It is the main cause of respiratory pasteurellosis in sheep and goats of all age groups.
BACKGROUND
species are frequently encountered as serious diseases in small ruminants. It is the main cause of respiratory pasteurellosis in sheep and goats of all age groups.
METHODS
The cross-sectional study was conducted from December 2022 to April 2023 in Haramaya district, eastern Ethiopia, to isolate and identify and and estimate their prevalence, associated risk factors, and antimicrobial sensitivity of isolates in small ruminants using a purposive sampling method. A total of 384 samples (156 nasal swabs from clinic cases and 228 lung swabs from abattoir cases) were collected. STATA 14 software was used to analyze the data. In addition, multivariable logistic regression analysis was performed to assess an association of risk factors.
RESULTS
Out of the 384 samples examined, 164 were positive for pasteurellosis, resulting in a 42.70% prevalence. Similarly, 63 (38.4%) of the 164 positive results were from nasal swabs, while 101 (61.6%) came from lung samples. accounted for 126 (76.82%) of the isolates, while accounted for 38 (23.17%). Of the 63 nasal swab isolates, 33 (37%) were from goats and 30 (42.8%) were from sheep. And 17 (10.89%) and 46 (29.58%), respectively, were and . Of the 46 (40%) of the 101 (44.3%) isolates of the pneumonic lung, samples were from goats, while 55 (48.47%) were from sheep. In this study, the risk factors (species, age, and body condition score) were found to be significant ( < 0.05). isolates evaluated for antibiotic susceptibility were highly resistant to oxacillin (90.90%), followed by gentamycin (72.72%), and penicillin (63.63%). However, the isolates were highly sensitive to chloramphenicol (90.90%), followed by tetracycline (63.63%), and ampicillin (54.54%).
CONCLUSION
This study showed that and are the common causes of mannheimiosis and pasteurellosis in small ruminants, respectively, and isolates were resistant to commonly used antibiotics in the study area. Thus, an integrated vaccination strategy, antimicrobial resistance monitoring, and avoidance of stress-inducing factors are recommended.
Topics: Animals; Pasteurella multocida; Mannheimia haemolytica; Ethiopia; Sheep; Goats; Anti-Bacterial Agents; Cross-Sectional Studies; Microbial Sensitivity Tests; Sheep Diseases; Goat Diseases; Prevalence; Risk Factors; Pasteurella Infections
PubMed: 38655561
DOI: 10.1155/2024/5605552 -
Scientific Reports Jun 2023Respiratory diseases caused by Mannheimia haemolytica (M. haemolytica) and Pasteurella multocida (P. multocida) have been known to result in a considerable loss due to...
Identification of serotypes of Mannheimia haemolytica and Pasteurella multocida from pneumonic cases of sheep and goats and their antimicrobial sensitivity profiles in Borana and Arsi zones, Ethiopia.
Respiratory diseases caused by Mannheimia haemolytica (M. haemolytica) and Pasteurella multocida (P. multocida) have been known to result in a considerable loss due to mortality and reduced production. This study aimed at isolation and identification of M. haemolytica and P. multocida associated with pneumonic pasteurellosis in sheep and goats using bacteriological and molecular techniques. Identification of serotypes of M. haemolytica and P. multocida was done using indirect haemagglutination test. The in vitro antimicrobial sensitivity profiles of the M. haemolytica were tested using standard disk diffusion method. A total of 52 and 78 nasal swabs were collected from pneumonic cases for bacterial isolation and identification in Borana and Arsi zone, respectively. Four hundred sera samples were collected for identification of serotypes. The results showed that 17 of 52 (32.69%; 95% CI 20.33, 47.11) nasal swabs collected from pneumonic animals in Borana yielded positive results for Pasteurella/Mannheimia species, 13 (25.00%; 95% CI 14.03, 38.95) of which were M. haemolytica. None of the samples yielded P. multocida. Twenty-three of 78 (29.49%; 95% CI 19.69, 40.89) nasal swabs collected at Arsi from pneumonic animals yielded positive results for M. haemolytica (17) and P. multocida (6). Secondary biochemical characterization revealed that 14 of the 17 isolates conform to M. haemolytica whereas none of the 6 isolates suspected to be P. mutocida were confirmed. Eleven (84.62%) isolates from Borana and 4 (28.57%) from Arsi were confirmed to be M. haemolytica using PCR targeting the Rpt2 genes. Assay for M. haemolytica serotype A1 revealed all belong to A1. None of the isolates with cultural and morphological features of P. multocida gave positive results by molecular assay. Serological assay identified three serotypes of M. haemolytica namely A1, A2 and A7 almost in all of the samples whereas P. multocida serotype A was detected in 78.75% of the samples. The M. haemolytica isolates tested for susceptibility to antibiotics showed resistance against Bacitracin (83.33%) and Penicillin (50.00%) while they were found susceptible to Gentamycin (100%), Chloramphenicol (100%) and Sulfamethoxazole (100%) and Tetracycline (83.33%). In conclusion, the results of the present study revealed the association of M. haemolytica with pneumonic pasteurellosis in sheep and goats and can be of use in vaccine development in Ethiopia. Nevertheless, further investigations and continuous monitoring of antimicrobial resistance and appropriate selection and prudent use of antimicrobials in livestock sector are required.
Topics: Cattle; Animals; Sheep; Mannheimia haemolytica; Pasteurella multocida; Pasteurellosis, Pneumonic; Serogroup; Ethiopia; Goats; Pasteurella; Anti-Bacterial Agents; Sheep Diseases
PubMed: 37268660
DOI: 10.1038/s41598-023-36026-2 -
Frontiers in Cellular and Infection... 2023Bovine respiratory disease (BRD) is the most devastating disease affecting beef and dairy cattle producers in North America. An emerging area of interest is the... (Meta-Analysis)
Meta-Analysis
BACKGROUND
Bovine respiratory disease (BRD) is the most devastating disease affecting beef and dairy cattle producers in North America. An emerging area of interest is the respiratory microbiome's relationship with BRD. However, results regarding the effect of BRD on respiratory microbiome diversity are conflicting.
RESULTS
To examine the effect of BRD on the alpha diversity of the respiratory microbiome, a meta-analysis analyzing the relationship between the standardized mean difference (SMD) of three alpha diversity metrics (Shannon's Diversity Index (Shannon), Chao1, and Observed features (OTUs, ASVs, species, and reads) and BRD was conducted. Our multi-level model found no difference in Chao1 and Observed features SMDs between calves with BRD and controls. The Shannon SMD was significantly greater in controls compared to that in calves with BRD. Furthermore, we re-analyzed 16S amplicon sequencing data from four previously published datasets to investigate BRD's effect on individual taxa abundances. Additionally, based on Bray Curtis and Jaccard distances, health status, sampling location, and dataset were all significant sources of variation. Using a consensus approach based on RandomForest, DESeq2, and ANCOM-BC2, we identified three differentially abundant amplicon sequence variants (ASVs) within the nasal cavity, ASV5_, ASV19_, and ASV37_ However, no ASVs were differentially abundant in the other sampling locations. Moreover, based on SECOM analysis, ASV37_ had a negative relationship with ASV1_, ASV5_, and ASV4_. ASV19_ had negative relationships with ASV1_, ASV4_, ASV54_, ASV7_, and ASV8_.
CONCLUSIONS
Our results confirm a relationship between bovine respiratory disease and respiratory microbiome diversity and composition, which provide additional insight into microbial community dynamics during BRD development. Furthermore, as sampling location and sample processing (dataset) can also affect results, consideration should be taken when comparing results across studies.
Topics: Cattle; Animals; Respiratory Tract Diseases; Cattle Diseases; Clostridiales; Microbiota; Mycoplasma hyorhinis
PubMed: 37743862
DOI: 10.3389/fcimb.2023.1223090 -
Veterinary Microbiology Jan 2024Mannheimia haemolytica is known as one of the major bacterial contributors to Bovine Respiratory Disease (BRD) syndrome. This study sought to establish a novel...
Mannheimia haemolytica is known as one of the major bacterial contributors to Bovine Respiratory Disease (BRD) syndrome. This study sought to establish a novel species-specific PCR to aid in identification of this key pathogen. As well, an existing multiplex PCR was used to determine the prevalence of serovars 1, 2 or 6 in Australia. Most of the 65 studied isolates originated from cattle with a total of 11 isolates from small ruminants. All problematic field isolates in the identification or serotyping PCRs were subjected to whole genome sequencing and bioinformatic analysis. The field isolates were also subjected to rep-PCR fingerprinting. A total of 59 out of the 65 tested isolates were conformed as M. haemolytica by the new species-specific PCR which is based on the rpoB gene. The confirmed M. haemolytica field isolates were assigned to serovars 1 (24 isolates), 2 (seven isolates) and 6 (26 isolates) while two of the isolates were negative in the serotyping PCR. The two non-typeable isolates were assigned to serovar 7 and 14 following whole genome sequencing and bioinformatic analysis. The rep-PCR typing resulted in five major clusters with serovars 1 and 6 often within the same cluster. The M. haemolytica-specific PCR developed in this work was species specific and should be a valuable support for frontline diagnostic laboratories. The serotyping results support the relative importance of serovars 1 and 6 in bovine respiratory disease.
Topics: Cattle; Animals; Mannheimia haemolytica; Bacteria; Serotyping; Cattle Diseases; Ruminants; Multiplex Polymerase Chain Reaction; Respiratory Tract Diseases
PubMed: 38086163
DOI: 10.1016/j.vetmic.2023.109930 -
Frontiers in Veterinary Science 2023Effective identification and treatment of bovine respiratory disease (BRD) is an ongoing health and economic issue for the dairy and beef cattle industries. Bacteria...
INTRODUCTION
Effective identification and treatment of bovine respiratory disease (BRD) is an ongoing health and economic issue for the dairy and beef cattle industries. Bacteria pathogens , , , and Histophilus somni and the virus Bovine herpesvirus-1 (BHV-1), Bovine parainfluenza-3 virus (BPIV-3), Bovine respiratory syncytial virus (BRSV), Bovine adenovirus 3 (BAdV3), bovine coronavirus (BoCV) and Bovine viral diarrhea virus (BVDV) have commonly been identified in BRD cattle; however, no studies have investigated the fungal community and how it may also relate to BRD.
METHODS
The objective of this study was to understand if the nasal mycobiome differs between a BRD-affected (n = 56) and visually healthy (n = 73) Holstein steers. Fungal nasal community was determined by using Internal Transcribed Spacer (ITS) sequencing.
RESULTS
The phyla, Ascomycota and Basidiomycota, and the genera, and , were the most abundant among all animals, regardless of health status. We identified differences between healthy and BRD animals in abundance of and at a sub-species level that could be a potential indicator of BRD. No differences were observed in the nasal fungal alpha and beta diversity between BRD and healthy animals. However, the fungal community structure was affected based on season, specifically when comparing samples collected in the summer to the winter season. We then performed a random forest model, based on the fungal community and abundance of the BRD-pathobionts (qPCR data generated from a previous study using the same animals), to classify healthy and BRD animals and determine the agreement with visual diagnosis. Classification of BRD or healthy animals using ITS sequencing was low and agreed with the visual diagnosis with an accuracy of 51.9%. A portion of the ITS-predicted BRD animals were not predicted based on the abundance of BRD pathobionts. Lastly, fungal and bacterial co-occurrence were more common in BRD animals than healthy animals.
DISCUSSION
The results from this novel study provide a baseline understanding of the fungal diversity and composition in the nasal cavity of BRD and healthy animals, upon which future interaction studies, including other nasal microbiome members to further understand and accurately diagnose BRD, can be designed.
PubMed: 37441557
DOI: 10.3389/fvets.2023.1165994 -
Genes Jun 2023Infertility incidence is rising worldwide, with male infertility accounting for about 50% of cases. To date, several factors have been associated with male infertility;...
Infertility incidence is rising worldwide, with male infertility accounting for about 50% of cases. To date, several factors have been associated with male infertility; in particular, it has been suggested that semen microbiota may play a role. Here, we report the NGS-based analyses of 20 semen samples collected from men with (Case) and without (Control) semen alterations. Genomic DNA was extracted from each collected sample, and a specific PCR was carried out to amplify the V4-V6 regions of the 16S rRNA. Sequence reactions were carried out on the MiSeq and analyzed by specific bioinformatic tools. We found a reduced richness and evenness in the Case versus the Control group. Moreover, specific genera, the , the and the were significantly increased in the Case compared to the Control group. Finally, we highlighted a correlation between the microbial profile and semen hyperviscosity. Even if further studies are required on larger groups of subjects to confirm these findings and explore mechanistic hypotheses, our results confirm the correlation between semen features and seminal microbiota. These data, in turn, may open the way to the possible use of semen microbiota as an attractive target for developing novel strategies for infertility management.
Topics: Humans; Male; Semen; Metagenomics; RNA, Ribosomal, 16S; Infertility, Male; Semen Analysis
PubMed: 37372408
DOI: 10.3390/genes14061228