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Pathogens (Basel, Switzerland) Nov 2023is a member of the family that has been reported sporadically. Although strains exhibiting multidrug-resistant profiles have been identified an in-depth genomic...
is a member of the family that has been reported sporadically. Although strains exhibiting multidrug-resistant profiles have been identified an in-depth genomic analysis focusing on antimicrobial resistance (AMR) has been lacking, and was therefore performed in this study. Forty-eight strains, isolated from humans, animals, foods, and the environment during 1970-2023, were analyzed. A large number of single-nucleotide polymorphisms were found, indicating a highly diverse population. Whilst strains were found to be circulating at the One Health interface, only human and environmental strains exhibited multidrug resistance genotypes. Sixty-one different antimicrobial resistance genes (ARGs) were identified, highlighting genes encoding mobile colistin resistance, carbapenemases, and extended-spectrum β-lactamases. Worryingly, the co-occurrence of , , , and , as well as , , and , was detected. Plasmid sequences were identified as carrying clinically important ARGs, evidencing IncX3 plasmids harboring , , or genes. Virulence genotyping underlined as being a low-virulence species. In this regard, is emerging as a new multidrug-resistant species belonging to the family. Therefore, continuous epidemiological genomic surveillance of is required.
PubMed: 38003800
DOI: 10.3390/pathogens12111335 -
MicrobiologyOpen Oct 2023Liquid soap dispensers are widely used in domestic and clinical settings. In previous studies, the risk of bacterial contamination of refillable systems was pointed out...
Liquid soap dispensers are widely used in domestic and clinical settings. In previous studies, the risk of bacterial contamination of refillable systems was pointed out and a bacterial contamination rate of 25%, with values of up to 10 colony-forming units/mL (CFU/mL), was reported. However, the route of contamination remains elusive. To address this point, we determined the microbial contamination of refillable standard pump dispensers and nonrefillable press-dispenser systems. Following the collection of 104 liquid soap dispensers from hotel rooms across Germany, bacterial counts were determined. Isolates of samples containing nonfastidious Gram-negative bacteria were further analyzed by the Vitek 2 system for the determination of species. 70.2% of the refillable pump dispensers (mean total bacterial count = 2.2 × 10 CFU/mL) but only 10.6% of the nonrefillable press dispensers, were contaminated (mean total bacterial count = 1.5 × 10 CFU/mL). Of samples containing nonfastidious Gram-negative bacteria, Pluralibacter gergoviae was present in 41.7%, Pseudomonads (Pseudomonas aeruginosa and Pseudomonas putida) in 25%, Serratia marcescens in 16.7%, and Klebsiella oxytoca and Pasteurella testudinis in 8.3%. After the initial assessment, we contaminated different dispensing systems with P. aeruginosa/P. gergoviae, to reveal the route of contamination and identied the pressure release of standard pump dispensers as the loophole for microbial contamination.
Topics: Soaps; Gram-Negative Bacteria; Bacterial Load; Serratia marcescens; Germany
PubMed: 37877653
DOI: 10.1002/mbo3.1384