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MicrobiologyOpen Oct 2023Liquid soap dispensers are widely used in domestic and clinical settings. In previous studies, the risk of bacterial contamination of refillable systems was pointed out...
Liquid soap dispensers are widely used in domestic and clinical settings. In previous studies, the risk of bacterial contamination of refillable systems was pointed out and a bacterial contamination rate of 25%, with values of up to 10 colony-forming units/mL (CFU/mL), was reported. However, the route of contamination remains elusive. To address this point, we determined the microbial contamination of refillable standard pump dispensers and nonrefillable press-dispenser systems. Following the collection of 104 liquid soap dispensers from hotel rooms across Germany, bacterial counts were determined. Isolates of samples containing nonfastidious Gram-negative bacteria were further analyzed by the Vitek 2 system for the determination of species. 70.2% of the refillable pump dispensers (mean total bacterial count = 2.2 × 10 CFU/mL) but only 10.6% of the nonrefillable press dispensers, were contaminated (mean total bacterial count = 1.5 × 10 CFU/mL). Of samples containing nonfastidious Gram-negative bacteria, Pluralibacter gergoviae was present in 41.7%, Pseudomonads (Pseudomonas aeruginosa and Pseudomonas putida) in 25%, Serratia marcescens in 16.7%, and Klebsiella oxytoca and Pasteurella testudinis in 8.3%. After the initial assessment, we contaminated different dispensing systems with P. aeruginosa/P. gergoviae, to reveal the route of contamination and identied the pressure release of standard pump dispensers as the loophole for microbial contamination.
Topics: Soaps; Gram-Negative Bacteria; Bacterial Load; Serratia marcescens; Germany
PubMed: 37877653
DOI: 10.1002/mbo3.1384 -
Antimicrobial Agents and Chemotherapy Oct 2022
Topics: Humans; Cross Infection; beta-Lactamases; Bacterial Proteins; Enterobacter; Anti-Bacterial Agents; Plasmids; Microbial Sensitivity Tests
PubMed: 36121219
DOI: 10.1128/aac.01093-22 -
Journal of Oral Microbiology 2023We assessed the level of evidence for the presence of new periodontal pathogens by (i) comparing the occurrence of non-classical periodontal taxa between healthy vs....
We assessed the level of evidence for the presence of new periodontal pathogens by (i) comparing the occurrence of non-classical periodontal taxa between healthy vs. periodontitis patients (Association study); (ii) assessing the modifications in the prevalence and levels of these species after treatments (Elimination study). In the Association study, we compared the prevalence and levels of 39 novel bacterial species between periodontally healthy and periodontitis patients. In the Elimination study, we analyzed samples from periodontitis patients assigned to receive scaling and root planing alone or with metronidazole+ amoxicillin TID/ 14 days. Levels of 79 bacterial species (39 novel and 40 classic) were assessed at baseline, 3 and 12 months post-therapy. All samples were analyzed using Checkerboard DNA-DNA hybridization. Out of the 39 novel species evaluated, eight were categorized as having strong and four as having moderate association with periodontitis. Our findings suggest strong evidence supporting , , , , , , , and , and moderate evidence supporting , , , and as periodontal pathogens. These findings contribute to a better understanding of the etiology of periodontitis and may guide future diagnostic and interventional studies.
PubMed: 37261036
DOI: 10.1080/20002297.2023.2213111 -
Pathogens (Basel, Switzerland) Nov 2023is a member of the family that has been reported sporadically. Although strains exhibiting multidrug-resistant profiles have been identified an in-depth genomic...
is a member of the family that has been reported sporadically. Although strains exhibiting multidrug-resistant profiles have been identified an in-depth genomic analysis focusing on antimicrobial resistance (AMR) has been lacking, and was therefore performed in this study. Forty-eight strains, isolated from humans, animals, foods, and the environment during 1970-2023, were analyzed. A large number of single-nucleotide polymorphisms were found, indicating a highly diverse population. Whilst strains were found to be circulating at the One Health interface, only human and environmental strains exhibited multidrug resistance genotypes. Sixty-one different antimicrobial resistance genes (ARGs) were identified, highlighting genes encoding mobile colistin resistance, carbapenemases, and extended-spectrum β-lactamases. Worryingly, the co-occurrence of , , , and , as well as , , and , was detected. Plasmid sequences were identified as carrying clinically important ARGs, evidencing IncX3 plasmids harboring , , or genes. Virulence genotyping underlined as being a low-virulence species. In this regard, is emerging as a new multidrug-resistant species belonging to the family. Therefore, continuous epidemiological genomic surveillance of is required.
PubMed: 38003800
DOI: 10.3390/pathogens12111335 -
Microbiology Resource Announcements Feb 2020In order to expand the limited understanding of the genomics of antimicrobial-resistant industrial bacteria, we report the genome sequence of ECO77, a historical...
In order to expand the limited understanding of the genomics of antimicrobial-resistant industrial bacteria, we report the genome sequence of ECO77, a historical contaminant strain of industrial origin. The multireplicon 6.16-Mbp genome of ECO77 consists of a 5.37-Mbp main chromosome, a megaplasmid (605,666 bp), and a large plasmid (182,007 bp).
PubMed: 32107303
DOI: 10.1128/MRA.01561-19 -
Microbiome Apr 2023Captive koala breeding programmes are essential for long-term species management. However, breeding efficacy is frequently impacted by high neonatal mortality rates in...
Characterisation of the koala (Phascolarctos cinereus) pouch microbiota in a captive population reveals a dysbiotic compositional profile associated with neonatal mortality.
BACKGROUND
Captive koala breeding programmes are essential for long-term species management. However, breeding efficacy is frequently impacted by high neonatal mortality rates in otherwise healthy females. Loss of pouch young typically occurs during early lactation without prior complications during parturition and is often attributed to bacterial infection. While these infections are thought to originate from the maternal pouch, little is known about the microbial composition of koala pouches. As such, we characterised the koala pouch microbiome across the reproductive cycle and identified bacteria associated with mortality in a cohort of 39 captive animals housed at two facilities.
RESULTS
Using 16S rRNA gene amplicon sequencing, we observed significant changes in pouch bacterial composition and diversity between reproductive time points, with the lowest diversity observed following parturition (Shannon entropy - 2.46). Of the 39 koalas initially sampled, 17 were successfully bred, after which seven animals lost pouch young (overall mortality rate - 41.18%). Compared to successful breeder pouches, which were largely dominated by Muribaculaceae (phylum - Bacteroidetes), unsuccessful breeder pouches exhibited persistent Enterobacteriaceae (phylum - Proteobacteria) dominance from early lactation until mortality occurred. We identified two species, Pluralibacter gergoviae and Klebsiella pneumoniae, which were associated with poor reproductive outcomes. In vitro antibiotic susceptibility testing identified resistance in both isolates to several antibiotics commonly used in koalas, with the former being multidrug resistant.
CONCLUSIONS
This study represents the first cultivation-independent characterisation of the koala pouch microbiota, and the first such investigation in marsupials associated with reproductive outcomes. Overall, our findings provide evidence that overgrowth of pathogenic organisms in the pouch during early development is associated with neonatal mortality in captive koalas. Our identification of previously unreported, multidrug resistant P. gergoviae strains linked to mortality also underscores the need for improved screening and monitoring procedures aimed at minimising neonatal mortality in future. Video Abstract.
Topics: Animals; Female; Bacteria; Microbiota; Phascolarctidae; RNA, Ribosomal, 16S; Dysbiosis
PubMed: 37060097
DOI: 10.1186/s40168-023-01527-9 -
Genome Announcements Dec 2014Pluralibacter gergoviae FB2, a bacterial strain isolated from packed food, has been found to exhibit quorum-quenching properties. Hence, we report the first, complete...
Pluralibacter gergoviae FB2, a bacterial strain isolated from packed food, has been found to exhibit quorum-quenching properties. Hence, we report the first, complete genome of P. gergoviae sequenced using the Pacific Biosciences single-molecule, real-time (SMRT) platform.
PubMed: 25502672
DOI: 10.1128/genomeA.01276-14