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Polymers Nov 2023The treatment and reuse of wastewater are crucial for the effective utilization and protection of global water resources. Polycyclic aromatic hydrocarbons (PAHs), as one...
The treatment and reuse of wastewater are crucial for the effective utilization and protection of global water resources. Polycyclic aromatic hydrocarbons (PAHs), as one of the most common organic pollutants in industrial wastewater, are difficult to remove due to their relatively low solubility and bioavailability in the water environment. However, biosurfactants with both hydrophilic and hydrophobic groups are effective in overcoming these difficulties. Therefore, a biosurfactant-producing strain MP-6 was isolated in this study to enhance the bioavailability and biodegradation of PAHs, especially high-molecular-weight PAHs (HMW-PAHs). FTIR and LC-MS analysis showed that the MP-6 surfactant belongs to rhamnolipids, a type of biopolymer, which can reduce the water surface tension from 73.20 mN/m to 30.61 mN/m at a critical micelle concentration (CMC = 93.17 mg/L). The enhanced solubilization and biodegradation of PAHs, particularly HMW-PAHs (when MP-6 was introduced), were also demonstrated in experiments. Furthermore, comprehensive environmental stress tolerance tests were conducted to confirm the robustness of the MP-6 biosurfactant, which signifies the potential adaptability and applicability of this biosurfactant in diverse environmental remediation scenarios. The results of this study, therefore, have significant implications for future applications in the treatment of wastewater containing HMW-PAHs, such as coking wastewater.
PubMed: 38232027
DOI: 10.3390/polym15234571 -
Toxics Dec 2023The use of bacteria of the genus -destructors of persistent pollutants for biotechnologies of environmental purification-is an interesting area of research. The aim of...
The use of bacteria of the genus -destructors of persistent pollutants for biotechnologies of environmental purification-is an interesting area of research. The aim of this work was to study the potential of strain 5(3) isolated from pesticide-contaminated soil as a degrader of C-C perfluorocarboxylic acids (PFCAs) and analyze its complete genome. The genome of the strain has been fully sequenced. It consists of a chromosome with a length of 5,676,241 b.p. and containing a total of 5134 genes, in particular, haloalkane dehalogenase gene (), haloacetate dehalogenase H-1 gene (), fluoride ion transporter gene () and alkanesulfonate monooxygenase gene (), responsible for the degradation of fluorinated compounds. The strain 5(3) for was cultivated for 7 days in a liquid medium with various C-C PFCAs as the sole source of carbon and energy, and completely disposed of them. The results of LC-MS analysis showed that the transformation takes place due to perfluorohexanoic acid with the release of various levels of stoichiometry (depending on PFCA) of fluorine ion mineralization indicators determined by ion chromatography. Thus, strain 5(3) demonstrates a genetically confirmed high potential for the decomposition of C-C PFCA.
PubMed: 38133402
DOI: 10.3390/toxics11121001 -
Microbiology Resource Announcements Dec 2023The P 5(3) strain is a potential degrader of persistent perfluorinated pollutants, particularly C-C perfluorinated acids. The genome of the strain has been fully...
The P 5(3) strain is a potential degrader of persistent perfluorinated pollutants, particularly C-C perfluorinated acids. The genome of the strain has been fully sequenced. It consists of a chromosome with a length of 5,676,241 base pairs and a G-C content of 64.38%.
PubMed: 37955621
DOI: 10.1128/MRA.00839-23