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Plant Physiology and Biochemistry : PPB Oct 2021Acetic acid priming may mitigate salt stress to plants by modulating lipid metabolism. Carex rigescens is a stress-tolerant turfgrass species with a widespread...
Acetic acid priming may mitigate salt stress to plants by modulating lipid metabolism. Carex rigescens is a stress-tolerant turfgrass species with a widespread distribution in north China. The objective of this study was to figure out whether modification of lipid profiles, including the contents, compositions and saturation levels of leaf lipids, may contribute to acetic acid modulated salt tolerance in C. rigescens. Plants of C. rigescens were primed with or without acetic acid (30 mM) and subsequently exposed to salt stress (300 mM NaCl) for 15 days. Salt stress affected the physiological performance of C. rigescens, while acetic acid-primed plants showed significantly lower malondialdehyde content, proline content, and electrolyte leakage than non-primed plants under salt stress. Acetic acid priming enhanced the contents of phospholipids and glycolipids involved in membrane stabilization and stress signaling (phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, digalactosyl diacylglycerol, monogalactosyl diacylglycerol, and sulfoquinovosyldiacylglycerol), reduced the content of toxic lipid intermediates (free fatty acids) during subsequent exposure to salt stress. Furthermore, expression levels of genes involved in lipid metabolism such as CK and PLDα changed due to acetic acid priming. These results demonstrated that acetic acid priming could enhance salt tolerance of C. rigescens by regulating lipid metabolism. The lipids could be used as biomarkers to select for salt-tolerant grass germplasm.
Topics: Acetic Acid; Carex Plant; Lipidomics; Salt Stress; Salt Tolerance; Stress, Physiological
PubMed: 34488152
DOI: 10.1016/j.plaphy.2021.08.045 -
Physiologia Plantarum Mar 2023Drought is an important stress factor that limits plant growth and development. Female willows generally display stronger drought tolerance than males. The application...
Drought is an important stress factor that limits plant growth and development. Female willows generally display stronger drought tolerance than males. The application of exogenous acetic acid (AA) has emerged as an efficient and eco-friendly approach to facilitate drought tolerance in willows. However, whether AA exerts sexually different effects on willows remains undefined. In this study, we comprehensively performed morphological and physiological analyses on three willow species, Salix rehderiana, Salix babylonica, and Salix matsudana, to investigate the sexually different responses to drought and AA. The results indicated that willow females were more drought-tolerant than males. AA application effectively enhanced willows' drought tolerance, and females applied with AA displayed greater root distribution and activity, stronger osmotic and antioxidant capacity and photosynthetic rate but less reactive oxygen species, or abscisic acid-mediated stomatal closure than males. In addition, AA application enhanced the jasmonic acid signaling pathway in females but inhibited it in males, conferring stronger drought defense capacity in female willows than in males. Overall, AA application improves drought tolerance more in female than in male willows, further enlarging the sexual differences in willows under drought-stressed conditions.
Topics: Salix; Drought Resistance; Acetic Acid; Antioxidants; Droughts
PubMed: 36917073
DOI: 10.1111/ppl.13890 -
Journal of Proteome Research Jan 2023Despite the general acceptance of formic acid as the additive of choice for peptide reversed-phase LC-MS/MS applications, some still argue that the selection of acetic...
Despite the general acceptance of formic acid as the additive of choice for peptide reversed-phase LC-MS/MS applications, some still argue that the selection of acetic acid represents a better option. To settle this debate, we investigated both the difference in MS sensitivity and chromatographic behavior of peptides between these two systems. This interlaboratory study was performed using different MS setups and C18 separation media employing both 0.1% formic and 0.5% acetic acid as ion pairing modifiers. Relative to formic acid, we find an overall ∼2.2-2.5× increase in MS signal and a slight decrease in RP LC retention (-0.7% acetonitrile on average) for acetic acid conditions. While these two features have opposing effects on peptide detectability, we find that acetic acid produces up to 60% higher peptide ID output depending on the type of sample. The drop in RPLC retention increases with peptide net charge at acidic pH. MS signal is dependent on the difference between the charge of the precursor ion and the charge of the peptide in solution, favoring species with a low pI. Lower peptide retention under acetic acid conditions demonstrates its higher hydrophilicity and, as expected, leads to composition and sequence-dependent character of the observed retention shift.
Topics: Chromatography, High Pressure Liquid; Chromatography, Liquid; Acetic Acid; Proteomics; Tandem Mass Spectrometry; Peptides
PubMed: 36480176
DOI: 10.1021/acs.jproteome.2c00388 -
Bioresource Technology Feb 2020The severe pretreatment of poplar makes xylan difficult to utilize efficiently. In this work, poplar was pretreated by hydrogen peroxide-acetic acid (HPAC) with HSO as...
The severe pretreatment of poplar makes xylan difficult to utilize efficiently. In this work, poplar was pretreated by hydrogen peroxide-acetic acid (HPAC) with HSO as catalyst to remove lignin, and the solid residues were used to produce xylooligosaccharides (XOS) and monosaccharides by two-step xylanase and cellulase hydrolysis. The results indicated that higher HSO concentrations in the HPAC pretreatment of poplar afforded stronger lignin removal ability. An increased XOS yield of 19.8% was obtained from 200 mM HSO-catalyzed poplar by xylanase and the XOS purity was high, with a very low xylose/XOS ratio of 0.14. Higher glucose (75.2%) and xylose (61.4%) yields were obtained from the HPAC-pretreated poplar using 50 mM HSO as catalyst. Finally, 16.9 g XOS and 296.4 g glucose were produced from 1 kg poplar by xylanase and cellulase. This study provides a method for producing functional XOS and monosaccharides from poplar using a simple reduced-pollution strategy.
Topics: Acetic Acid; Glucuronates; Hydrogen Peroxide; Hydrolysis; Monosaccharides; Oligosaccharides
PubMed: 31708384
DOI: 10.1016/j.biortech.2019.122349 -
Journal of Cardiovascular Magnetic... May 2021Low-field (0.55 T) high-performance cardiovascular magnetic resonance (CMR) is an attractive platform for CMR-guided intervention as device heating is reduced around...
PURPOSE
Low-field (0.55 T) high-performance cardiovascular magnetic resonance (CMR) is an attractive platform for CMR-guided intervention as device heating is reduced around 7.5-fold compared to 1.5 T. This work determines the feasibility of visualizing cardiac radiofrequency (RF) ablation lesions at low field CMR and explores a novel alternative method for targeted tissue destruction: acetic acid chemoablation.
METHODS
N = 10 swine underwent X-ray fluoroscopy-guided RF ablation (6-7 lesions) and acetic acid chemoablation (2-3 lesions) of the left ventricle. Animals were imaged at 0.55 T with native contrast 3D-navigator gated T1-weighted T1w) CMR for lesion visualization, gated single-shot imaging to determine potential for real-time visualization of lesion formation, and T1 mapping to measure change in T1 in response to ablation. Seven animals were euthanized on ablation day and hearts imaged ex vivo. The remaining animals were imaged again in vivo at 21 days post ablation to observe lesion evolution.
RESULTS
Chemoablation lesions could be visualized and displayed much higher contrast than necrotic RF ablation lesions with T1w imaging. On the day of ablation, in vivo myocardial T1 dropped by 19 ± 7% in RF ablation lesion cores, and by 40 ± 7% in chemoablation lesion cores (p < 4e-5). In high resolution ex vivo imaging, with reduced partial volume effects, lesion core T1 dropped by 18 ± 3% and 42 ± 6% for RF and chemoablation, respectively. Mean, median, and peak lesion signal-to-noise ratio (SNR) were all at least 75% higher with chemoablation. Lesion core to myocardium contrast-to-noise (CNR) was 3.8 × higher for chemoablation. Correlation between in vivo and ex vivo CMR and histology indicated that the periphery of RF ablation lesions do not exhibit changes in T1 while the entire extent of chemoablation exhibits T1 changes. Correlation of T1w enhancing lesion volumes indicated in vivo estimates of lesion volume are accurate for chemoablation but underestimate extent of necrosis for RF ablation.
CONCLUSION
The visualization of coagulation necrosis from cardiac ablation is feasible using low-field high-performance CMR. Chemoablation produced a more pronounced change in lesion T1 than RF ablation, increasing SNR and CNR and thereby making it easier to visualize in both 3D navigator-gated and real-time CMR and more suitable for low-field imaging.
Topics: Acetic Acid; Animals; Catheter Ablation; Myocardium; Predictive Value of Tests; Radiofrequency Ablation; Swine
PubMed: 33952312
DOI: 10.1186/s12968-020-00693-1 -
Bioprocess and Biosystems Engineering Dec 2022Direct reutilization of condensate can inhibit ethanol fermentation, 2-phenylethyl alcohol and furfural existed in the condensate are considered to be inhibitors. To...
Direct reutilization of condensate can inhibit ethanol fermentation, 2-phenylethyl alcohol and furfural existed in the condensate are considered to be inhibitors. To achieve the reutilization of the condensate, the ozonation combined with ion-exchange method was used. The results showed that the elimination rates of 2-phenylethyl alcohol and furfural reached 98.0% and 100.0%, respectively after ozonation, while the concentrations of acetic acid, propionic acid, butyric acid and valeric acid increased by 14.9%, 7.7%, 35.3% and 25.5%, respectively. The fermentation results showed that the inhibition of the condensate after ozonation was alleviated but was not completely eliminated. When the effluent volume treated by the ion-exchange method reached 80 BV, the concentrations of acetic acid, propionic acid, butyric acid and valeric acid decreased by 25.8%, 8.6%, 6.5% and 34.4%, respectively. The fermentation results showed that the inhibition of the condensate was completely eliminated after ozonation combined with ion-exchange treatment.
Topics: Fermentation; Furaldehyde; Butyric Acid; Phenylethyl Alcohol; Ethanol; Acetic Acid; Technology; Ozone
PubMed: 36264370
DOI: 10.1007/s00449-022-02782-0 -
Water Research Aug 2023In this study, a novel water treatment process combining permanganate (Mn(VII)) and peracetic acid (PAA, CHC(O)OOH) was employed to degrade sulfamethazine (SMT), a...
In this study, a novel water treatment process combining permanganate (Mn(VII)) and peracetic acid (PAA, CHC(O)OOH) was employed to degrade sulfamethazine (SMT), a typical model contaminant. Simultaneous application of Mn(VII) and a small amount of PAA resulted in much faster oxidation of organics than a single oxidant. Interestingly, coexistent acetic acid played a crucial role in SMT degradation, while background hydrogen peroxide (HO) had a negligible effect. However, compared with acetic acid, PAA could better improve the oxidation performance of Mn(VII) and accelerate the removal of SMT more significantly. The mechanism of SMT degradation by Mn(VII)-PAA process was systematically evaluated. Firstly, based on the quenching experiments, electron spin resonance (EPR) results and UV-visible spectrum, singlet oxygen (O), Mn(III) and MnO colloids were the predominant active substances, while organic radicals (R-O) showed negligible contribution. Then, the decay of Mn(VII) in the presence of PAA and HO was investigated. It was found that the coexisting HO accounted for almost all the decay of Mn(VII), PAA and acetic acid both had low reactivity toward Mn(VII). During the degradation process, acetic acid was able to acidify Mn(VII) and simultaneously acted as a ligand to form reactive complexes, while PAA mainly played a role of spontaneously decomposing to produce O, they jointly promoted the mineralization of SMT. Finally, the degradation intermediates of SMT and their toxicities were analyzed. This paper reported the Mn(VII)-PAA water treatment process for the first time, which provided a promising approach for rapid decontamination of refractory organics-polluted water.
Topics: Oxides; Manganese Compounds; Peracetic Acid; Sulfamethazine; Hydrogen Peroxide; Water Pollutants, Chemical; Oxidation-Reduction; Acetic Acid
PubMed: 37413749
DOI: 10.1016/j.watres.2023.120298 -
Environmental Technology Jun 2024Designed to meet the specific needs of the printing industry exhaust gas emissions, this paper proposes a method for the degradation of gaseous acetic acid ester...
Designed to meet the specific needs of the printing industry exhaust gas emissions, this paper proposes a method for the degradation of gaseous acetic acid ester organics that is environmentally friendly, safe, and simple to use: micro-nano cavitation technology. In the process of using micro-nano cavitation technology to degrade acetic acid ester organics, the products in the degradation process were analyzed by gas chromatography-mass (GC-MS) spectrometry, and the degradation pathways of acetic acid ester organics were identified. Under high temperatures and high pressure caused by cavitation collapse, the C-C bond and C-O bond on the main chain of organic matter are cleaved to form low molecular products. Low-molecular intermediate products are continuously produced as the reaction advances, and these intermediate products are further oxidized and decomposed into carbon dioxide and water. Besides, the factors that influence the degradation rate of acetic acid ester organics were investigated. Based on the experimental data, acetic acid esters can degrade with the greatest efficiency when their initial concentration is 200 ± 50 mg/m and their treatment time is 20∼30 min. Moreover, the experiment was optimized using the response surface method. The results suggested that for an initial concentration of 155.544 mg/m and a reaction time of 21.961 min, the best degradation rate was 0.251 min. Micro-nano cavitation technology is a novel and promising technology for the degradation of volatile organic compounds, with a wide range of practical applications.
Topics: Esters; Acetic Acid; Kinetics; Air Pollutants; Gas Chromatography-Mass Spectrometry
PubMed: 36855898
DOI: 10.1080/09593330.2023.2185819 -
Applied Microbiology and Biotechnology May 2021Acetic acid bacteria (AAB) are valuable biocatalysts for which there is growing interest in understanding their basics including physiology and biochemistry. This is... (Review)
Review
Acetic acid bacteria (AAB) are valuable biocatalysts for which there is growing interest in understanding their basics including physiology and biochemistry. This is accompanied by growing demands for metabolic engineering of AAB to take advantage of their properties and to improve their biomanufacturing efficiencies. Controlled expression of target genes is key to fundamental and applied microbiological research. In order to get an overview of expression systems and their applications in AAB, we carried out a comprehensive literature search using the Web of Science Core Collection database. The Acetobacteraceae family currently comprises 49 genera. We found overall 6097 publications related to one or more AAB genera since 1973, when the first successful recombinant DNA experiments in Escherichia coli have been published. The use of plasmids in AAB began in 1985 and till today was reported for only nine out of the 49 AAB genera currently described. We found at least five major expression plasmid lineages and a multitude of further expression plasmids, almost all enabling only constitutive target gene expression. Only recently, two regulatable expression systems became available for AAB, an N-acyl homoserine lactone (AHL)-inducible system for Komagataeibacter rhaeticus and an L-arabinose-inducible system for Gluconobacter oxydans. Thus, after 35 years of constitutive target gene expression in AAB, we now have the first regulatable expression systems for AAB in hand and further regulatable expression systems for AAB can be expected. KEY POINTS: • Literature search revealed developments and usage of expression systems in AAB. • Only recently 2 regulatable plasmid systems became available for only 2 AAB genera. • Further regulatable expression systems for AAB are in sight.
Topics: Acetic Acid; Acetobacteraceae; Gene Expression; Metabolic Engineering
PubMed: 33856535
DOI: 10.1007/s00253-021-11269-z -
Indian Journal of Cancer 2023Diagnostic adjuncts such as toluidine blue have been investigated as screening tools that improve visual examination of potentially malignant disorders (PMD) and oral...
BACKGROUND
Diagnostic adjuncts such as toluidine blue have been investigated as screening tools that improve visual examination of potentially malignant disorders (PMD) and oral cancer. Acetic acid has been reported to be of value in the early detection of cervical cancers. This study assessed the utility of 5% acetic acid as a diagnostic adjunct in oral PMD and compared the accuracy of acetic acid with toluidine blue in the detection of dysplastic PMD and high-risk lesions.
MATERIALS AND METHODS
This cross-sectional study was conducted at a dental hospital in a rural setting. Thirty-one patients with oral PMD formed the study group. Five percent acetic acid was applied to the lesions, followed by toluidine blue application and biopsy. Sensitivity, specificity, and positive and negative predictive values were computed considering true positives as stain uptake in dysplastic and high-risk PMD.
RESULTS
The sensitivity, specificity, and positive and negative predictive values of acetic acid for identifying dysplastic or malignant lesions were 100%, 13.3%, 51.2%, and 100%, respectively, and that for toluidine blue were 75%, 100%, 100%, and 78.9%, respectively. The corresponding values for identifying high-risk PMD (lesions with moderate and severe dysplasia) using acetic acid were 100%, 9.1%, 25.9%, and 100%, respectively, and for toluidine blue were 85.7%, 81.8%, 60%, and 94.7%, respectively.
CONCLUSION
The utility of acetic acid in detecting dysplasia and high-risk PMD is severely limited due to its poor specificity. Compared with acetic acid, toluidine blue is a superior screening tool.
Topics: Humans; Tolonium Chloride; Acetic Acid; Cross-Sectional Studies; Precancerous Conditions; Mouth Diseases
PubMed: 36861696
DOI: 10.4103/ijc.IJC_42_21