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BMC Oral Health May 2022Sex hormones influence circulation, periodontitis, and wound healing. The aim of the study was to compare the endothelium-dependent and independent vasodilation in human...
BACKGROUND
Sex hormones influence circulation, periodontitis, and wound healing. The aim of the study was to compare the endothelium-dependent and independent vasodilation in human gingiva in men and women.
METHODS
Gingival blood flow was evaluated in twelve male and twelve female subjects with healthy gingiva and no systemic conditions after acetylcholine or nitric oxide donor (NitroPOHL). Agonists were administered into the gingival sulcus at the right secondary incisor (test site). Regional gingival blood flow (GBF) was imaged by Laser Speckle Contrast Imager from the marginal gingiva to the mucogingival junction in four consecutive regions (coronal, midway1, midway2 and apical). Blood flow was expressed in Laser Speckle Perfusion Unit (LSPU). The absolute maximal blood flow change (Dmax), the area under the blood flow curve (AUC), and the time to peak (TTP) were calculated.
RESULTS
Males had higher baseline GBF than females (257 ± 18.2 vs. 225 ± 18.8 LSPU, p < 0.001). Acetylcholine and NitroPOHL significantly increased the GBF in all test regions. The Dmax after the acetylcholine was reduced apically compared to the coronal (90 ± 13 LSPU vs. 117 ± 7 LSPU, p < 0.01), but it was similar after NitroPOHL (78 ± 9 LSPU vs. 86 ± 6 LSPU, p = 0.398) in both sexes. The Dmax and AUC were higher, and the TTP was smaller in men in most regions after acetylcholine but not after NitroPOHL.
CONCLUSION
In the human gingiva, the endothelium-independent vasodilation propagates without attenuation in the line of the vascular supply in both sexes. At the same time, the endothelium-dependent ascending vasodilation attenuates similarly in men and women. However, men had more pronounced endothelium-dependent vasodilation than women. Therefore, it might contribute to the increased severity of periodontal disease in men.
TRIAL REGISTRATION
The study was registered with ClinicalTrials.gov on 09.06.2021 (NCT04918563).
Topics: Acetylcholine; Endothelium; Female; Gingiva; Humans; Male; Regional Blood Flow; Vasodilation
PubMed: 35562729
DOI: 10.1186/s12903-022-02186-2 -
The Journal of Contemporary Dental... Nov 2021To evaluate morphologic changes in the main foramen after enlargement with four different systems.
AIM AND OBJECTIVE
To evaluate morphologic changes in the main foramen after enlargement with four different systems.
MATERIALS AND METHODS
One hundred and twenty canals (buccal of maxillary molars and mesial of mandibular molars) with patency and apical foramen ≤200 µm were included. These apical foramina were photographed and then randomly divided into four experimental groups, according to the ProDesign Logic (PDL), ProDesign R (PDR), twisted file adaptive (TFA), and WaveOne Gold (WOG) systems, all of which were composed of three sequential instruments (Glidepath, #25 and #35). Root canals were prepared 1 mm beyond the main foramen ( = 30). The specimens were photographed again to capture intermediate (#25) and final enlargement (#35) images. These were analyzed using an image software that allowed comparison of the enlarged area and shape of the main foramen.
RESULTS
In the Intragroup comparison, the Friedman's test showed a statistical difference between each phase of foramen enlargement ( <0.05). In the intergroup comparison, the percentage of foramen enlargement showed statistically significant difference between Groups PDR and TFA only in the analysis from #25 to #35, with 42.88 and 67.34% of foramen area widening, respectively. Relative to the final foramen shape, 81.7% were observed to be circular, 17.5% oval, and 0.8% flattened.
CONCLUSION
Our results showed that foramen enlargement allowed an increase in area, maintained the circular shape of the foramen in the majority of cases, irrespective of the system used, and was a feasible option for use during preparation of the root canal system.
Topics: Dental Pulp Cavity; Equipment Design; Nickel; Root Canal Preparation; Titanium
PubMed: 35343446
DOI: No ID Found -
Australian Endodontic Journal : the... Aug 2019The aim of this study was to investigate the size, shape and position of the physiological foramen and the relationship between the age and gender to the location and...
The aim of this study was to investigate the size, shape and position of the physiological foramen and the relationship between the age and gender to the location and size of the apical foramen of mandibular first premolars. One hundred and one extracted human mandibular first premolar teeth were examined; age and gender of the patients were recorded. Apices of teeth were examined stereomicroscopically for number, size and shape of apical foramina; frequency and deviation of accessory foramina from the apex and the relation with age and gender. Sixty-four of the premolars had one foramen and 23% had two. The distances from the foramina openings to the apices ranged from 0.07 to 2.18 mm. In younger range irregular shape was dominant (41%), whilst in older range, round shape was dominant (45%). The shape of the apical foramen changed with age and the distance from the foramen to the anatomical apex increased with age.
Topics: Aged; Bicuspid; Humans; Mandible; Tooth Apex
PubMed: 30402906
DOI: 10.1111/aej.12313 -
Current Opinion in Cell Biology Oct 2021Epithelial cells use the process of extrusion to promote cell death while preserving a tight barrier. To extrude, a cell and its neighbors contract actin and myosin... (Review)
Review
Epithelial cells use the process of extrusion to promote cell death while preserving a tight barrier. To extrude, a cell and its neighbors contract actin and myosin circumferentially and basolaterally to seamlessly squeeze it out of the epithelium. Recent research highlights how early apical pulsatile contractions within the extruding cell might orchestrate contraction in three dimensions so that a cell extrudes out apically. Along with apical constrictions, studies of ion channels and mathematical modeling reveal how differential contraction between cells helps select specific cells to extrude. In addition, several studies have offered new insights into pathways that use extrusion to eliminate transformed cells or cause an aberrant form of extrusion that promotes cell invasion.
Topics: Actins; Epithelial Cells; Epithelium; Humans; Neoplasms; Signal Transduction
PubMed: 34034216
DOI: 10.1016/j.ceb.2021.04.005 -
International Journal of Clinical... 2023The success of endodontic treatment in open apex teeth depends on the establishment of an ideal apical barrier so that apical canal space between the periodontium and...
AIMS AND BACKGROUND
The success of endodontic treatment in open apex teeth depends on the establishment of an ideal apical barrier so that apical canal space between the periodontium and the root canal system is filled. Biodentine is a calcium silicate-based cement having several advantages over mineral trioxide aggregate (MTA), glass ionomer cement (GIC), and calcium hydroxide in the treatment of teeth with open apices. Orthograde delivery of biodentine is technique-sensitive and requires multiple radiographs for verification, as well as retrograde placement of biodentine requires surgical intervention. To use the advantages of biodentine and to overcome the disadvantages of placement techniques, an innovative technique was done to obturate the tooth with an open apex using a custom-fit prefabricated BioRoot inlay in this case report.
CASE DESCRIPTION
In the present case, biodentine has been used as a BioRoot inlay to overcome the disadvantages of various management options of open apex with parallel dentinal walls. BioRoot inlay is an intraradicular custom-made prefabricated restoration which provides the three-dimensional seal of the root canal space and promotes an apical barrier formation in a wide-open apex. This BioRoot inlay, placed passively in the canal, had a good seal laterally and apically along with the Biodentine as sealer forming a monoblock. This sealer helps in sealing milder discrepancies between the plug and the root, providing a three-dimensional seal which had resulted in good healing of periradicular bone.
CONCLUSION
Biodentine, when used as BioRoot inlay, has been shown to induce faster periapical healing. It provides promising results when used in open apices with parallel walls. It establishes three-dimensional obturation and proper apical seal, which promotes effective root-end induction.
CLINICAL SIGNIFICANCE
It can be considered as the effective and definitive alternative for nonsurgical and surgical approaches to treating open apex.
HOW TO CITE THIS ARTICLE
Thiyagarajan G, Manoharan M, Veerabadhran MM, Biodentine as BioRoot Inlay: A Case Report. Int J Clin Pediatr Dent 2023;16(2):400-404.
PubMed: 37519983
DOI: 10.5005/jp-journals-10005-2580 -
BioRxiv : the Preprint Server For... Apr 2023During development, multicellular rosettes serve as important cellular intermediates in the formation of diverse organ systems. Multicellular rosettes are transient...
During development, multicellular rosettes serve as important cellular intermediates in the formation of diverse organ systems. Multicellular rosettes are transient epithelial structures that are defined by the apical constriction of cells towards the rosette center. Due to the important role these structures play during development, understanding the molecular mechanisms by which rosettes are formed and maintained is of high interest. Utilizing the zebrafish posterior lateral line primordium (pLLP) as a model system, we identify the RhoA GEF Mcf2lb as a regulator of rosette integrity. The pLLP is a group of ~150 cells that migrates along the zebrafish trunk and is organized into epithelial rosettes; these are deposited along the trunk and will differentiate into sensory organs called neuromasts (NMs). Using single-cell RNA sequencing and whole-mount in situ hybridization, we showed that is expressed in the pLLP during migration. Given the known role of RhoA in rosette formation, we asked whether Mcf2lb plays a role in regulating apical constriction of cells within rosettes. Live imaging and subsequent 3D analysis of mutant pLLP cells showed disrupted apical constriction and subsequent rosette organization. This in turn resulted in a unique posterior Lateral Line phenotype: an excess number of deposited NMs along the trunk of the zebrafish. Cell polarity markers ZO-1 and Par-3 were apically localized, indicating that pLLP cells are normally polarized. In contrast, signaling components that mediate apical constriction downstream of RhoA, Rock-2a and non-muscle Myosin II were diminished apically. Altogether our results suggest a model whereby Mcf2lb activates RhoA, which in turn activates downstream signaling machinery to induce and maintain apical constriction in cells incorporated into rosettes.
PubMed: 37131612
DOI: 10.1101/2023.04.19.537573 -
Scientific Reports Jul 2022The retinal pigment epithelium (RPE) is a polarized monolayer that secretes growth factors and cytokines towards the retina apically and the choroid basolaterally....
The retinal pigment epithelium (RPE) is a polarized monolayer that secretes growth factors and cytokines towards the retina apically and the choroid basolaterally. Numerous RPE secreted proteins have been linked to the pathogenesis of age-related macular degeneration (AMD). The purpose of this study was to determine the differential apical and basolateral secretome of RPE cells, and the effects of oxidative stress on directional secretion of proteins linked to AMD and angiogenesis. Tandem mass tag spectrometry was used to profile proteins in human iPSC-RPE apical and basolateral conditioned media. Changes in secretion after oxidative stress induced by HO or tert-butyl hydroperoxide (tBH) were investigated by ELISA and western analysis. Out of 926 differentially secreted proteins, 890 (96%) were more apical. Oxidative stress altered the secretion of multiple factors implicated in AMD and neovascularization and promoted a pro-angiogenic microenvironment by increasing the secretion of pro-angiogenic molecules (VEGF, PTN, and CRYAB) and decreasing the secretion of anti-angiogenic molecules (PEDF and CFH). Apical secretion was impacted more than basolateral for PEDF, CRYAB and CFH, while basolateral secretion was impacted more for VEGF, which may have implications for choroidal neovascularization. This study lays a foundation for investigations of dysfunctional RPE polarized protein secretion in AMD and other chorioretinal degenerative disorders.
Topics: Angiogenesis Inducing Agents; Cells, Cultured; Humans; Hydrogen Peroxide; Induced Pluripotent Stem Cells; Macular Degeneration; Oxidative Stress; Retinal Pigment Epithelium; Vascular Endothelial Growth Factor A
PubMed: 35882889
DOI: 10.1038/s41598-022-16701-6 -
The EMBO Journal Dec 2023Apical-basal polarity is maintained by distinct protein complexes that reside in membrane junctions, and polarity loss in monolayered epithelial cells can lead to...
Apical-basal polarity is maintained by distinct protein complexes that reside in membrane junctions, and polarity loss in monolayered epithelial cells can lead to formation of multilayers, cell extrusion, and/or malignant overgrowth. Yet, how polarity loss cooperates with intrinsic signals to control directional invasion toward neighboring epithelial cells remains elusive. Using the Drosophila ovarian follicular epithelium as a model, we found that posterior follicle cells with loss of lethal giant larvae (lgl) or Discs large (Dlg) accumulate apically toward germline cells, whereas cells with loss of Bazooka (Baz) or atypical protein kinase C (aPKC) expand toward the basal side of wildtype neighbors. Further studies revealed that these distinct multilayering patterns in the follicular epithelium were determined by epidermal growth factor receptor (EGFR) signaling and its downstream target Pointed, a zinc-finger transcription factor. Additionally, we identified Rho kinase as a Pointed target that regulates formation of distinct multilayering patterns. These findings provide insight into how cell polarity genes and receptor tyrosine kinase signaling interact to govern epithelial cell organization and directional growth that contribute to epithelial tumor formation.
Topics: Animals; Cell Polarity; Drosophila melanogaster; Drosophila Proteins; Epithelial Cells; Epithelium; ErbB Receptors
PubMed: 37953688
DOI: 10.15252/embj.2023113856 -
Primary Dental Journal Mar 2023This article will provide a summary of causes of incomplete root end closure and the implications of this on endodontic treatment. The limitations and benefits of... (Review)
Review
This article will provide a summary of causes of incomplete root end closure and the implications of this on endodontic treatment. The limitations and benefits of traditional and more contemporary materials including the use of bioceramic cements are considered. The apical barrier technique is described in detail for teeth with immature apices utilising a variety of bioceramic cements and regenerative endodontic procedures with an overview of appropriate instruments to successfully complete the procedure.
Topics: Humans; Dental Pulp Necrosis; Dentition, Permanent; Root Canal Therapy
PubMed: 36916627
DOI: 10.1177/20501684231154337 -
Journal of Cell Science Mar 2024Primary cilia are sensory cellular organelles crucial for organ development and homeostasis. Ciliogenesis in polarized epithelial cells requires Rab19-mediated clearing...
Primary cilia are sensory cellular organelles crucial for organ development and homeostasis. Ciliogenesis in polarized epithelial cells requires Rab19-mediated clearing of apical cortical actin to allow the cilium to grow from the apically docked basal body into the extracellular space. Loss of the lysosomal membrane-tethering homotypic fusion and protein sorting (HOPS) complex disrupts this actin clearing and ciliogenesis, but it remains unclear how the ciliary function of HOPS relates to its canonical function in regulating late endosome-lysosome fusion. Here, we show that disruption of HOPS-dependent lysosomal fusion indirectly impairs actin clearing and ciliogenesis by disrupting the targeting of Rab19 to the basal body, and that this effect is specific to polarized epithelial cells. We also find that Rab19 functions in endolysosomal cargo trafficking in addition to having its previously identified role in ciliogenesis. In summary, we show that inhibition of lysosomal fusion leads to the abnormal accumulation of Rab19 on late endosomes, thus depleting Rab19 from the basal body and thereby disrupting Rab19-mediated actin clearing and ciliogenesis in polarized epithelial cells.
Topics: Actins; Cell Movement; Lysosomes; Protein Transport; Epithelial Cells
PubMed: 37665101
DOI: 10.1242/jcs.261047