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Burns : Journal of the International... Sep 2021In some cases, the tongue and oesophagus tissues are damaged by the corrosive burn. Surgical interventions may cause scar formation, and severe burns treatment methods...
INTRODUCTION
In some cases, the tongue and oesophagus tissues are damaged by the corrosive burn. Surgical interventions may cause scar formation, and severe burns treatment methods are limited. This study aims to investigate bromelain, a phytotherapeutic product, on the corrosive burn as a non-surgical option and as an adjunctive therapy, insofar as the treatment of corrosive wounds is not limited only to the treatment of oxidative stress and inflammatory reactions.
METHODS
On the tongues of Wistar albino rats, chemically produced oral ulcers were created by topical application of NaOH (40%) solution, and in the distal oesophagus same mixture was applied to produce a corrosive oesophageal burn. For a week, they were treated orally by bromelain (100 mg/kg/day) or saline solution. At the end of seven days, animals were decapitated to remove the tongue and oesophagus, and blood samples were collected to obtain serum. Myeloperoxidase (MPO) activity, malondialdehyde (MDA), glutathione (GSH), interleukin-1 beta (IL-1β) and tumour necrosis factor-alpha (TNF-α) concentrations were measured in serum, and luminol and lucigenin chemiluminescence (CL) were measured in tissue samples.
RESULTS
MDA and CL values were significantly increased, and GSH levels in tissue significantly decreased due to the corrosive burns. Saline treated corrosive burn group measured higher in the serum cytokines in according to the control group.
CONCLUSIONS
Bromelain administration decreased oxidant and inflammatory parameters and increased antioxidant levels in NaOH-induced corrosive burns. Thus, we concluded that bromelain may protect the tongue and oesophagus tissues with its anti-inflammatory and antioxidant effects.
Topics: Animals; Antioxidants; Bromelains; Burns; Caustics; Esophagus; Glutathione; Interleukin-1beta; Malondialdehyde; Peroxidase; Rats; Rats, Wistar; Sodium Hydroxide; Tumor Necrosis Factor-alpha
PubMed: 33934907
DOI: 10.1016/j.burns.2020.12.006 -
Journal of Healthcare Engineering 2021With the continuous popularization of smart medicine, the protective effect of silibinin in the liver has attracted much attention. This study mainly explores the liver...
With the continuous popularization of smart medicine, the protective effect of silibinin in the liver has attracted much attention. This study mainly explores the liver protection mechanism and absorption promotion technology of silybin based on intelligent medical analysis. Refining of silibinin: accurately weigh 1.0 g of silibinin in a three-necked flask; gradually add 50 mL of anhydrous methanol, reflux and filter the precipitated solid; and weigh it after drying. ICR male mice were taken as experimental subjects and randomly divided into groups of 10 each. The mice in the normal group and the model group were given intragastrically with 0.5% CMC-Na solution; the mice in the silibinin group were given intragastrically with SB/CMC-Na suspension; the mice in the remaining groups were given low, medium, and high-dose suspensions to their stomachs, and silibinin 23 acylate/CMC-Na suspension was administered at a dose of 10 mL/kg for 7 consecutive days. After that, the mice were fasted for 12 hours. After 6 hours of fasting (18 hours after modeling), the blood cells from their orbits were taken, placed in a 37°C water bath for 30 minutes, and centrifuged at 4000 rpm for 10 minutes, and then the serum was taken; the activity equivalent of AST and ALT in serum was measured; serum determination Medium AST and ALT vitality. The mice were killed by decapitation, fresh liver tissue was immediately collected, and part of it was frozen in liquid nitrogen for the RT-PCR test. The hepatocyte expansion and death were observed using a transmission electron microscope, and the oncosis index (OI) was calculated. Another part of the liver tissue was fixed in 4% paraformaldehyde solution, embedded in paraffin, dehydrated, and sliced at 4 m. Some sections were stained with conventional HE, and the pathological changes of liver cells were observed under light microscope; some sections were subjected to immunohistochemistry. Only one mouse died when 240 mg/kg of silibinin was given 10 minutes after the model was modeled. However, when 240 mg/kg silibinin was given to the mice 20 minutes after modeling, the mortality rate of the mice rose to 50%, and the therapeutic effect was significantly weakened. This research is helpful to advance the research of silybin in liver protection.
Topics: Animals; Humans; Liver; Male; Mice; Mice, Inbred ICR; Random Allocation; Silybin; Technology
PubMed: 34285784
DOI: 10.1155/2021/9968016 -
PeerJ 2023The chrysanthemum gene, homologous with tomato , is one of the earliest expressed genes controlling axillary meristem initiation. In this study, the wild-type...
The chrysanthemum gene, homologous with tomato , is one of the earliest expressed genes controlling axillary meristem initiation. In this study, the wild-type chrysanthemum (CW) and -overexpressed line 15 (C15) were used to investigate the regulatory mechanism of axillary bud development in chrysanthemum. Transcriptome sequencing was carried out to detect the differentially expressed genes of the axillary buds 0 h, 24 h and 48 h after decapitation. The phenotypic results showed that the number of axillary buds of C15 was significantly higher than CW. A total of 9,224 DEGs were identified in C15-0 CW-0, 10,622 DEGs in C15-24 CW-24, and 8,929 DEGs in C15-48 CW-48.GO and KEGG pathway enrichment analyses showed that the genes of the flavonoid, phenylpropanoids and plant hormone pathways appeared to be differentially expressed, indicating their important roles in axillary bud germination. reduces GA content in axillary buds by promoting GA2ox expression.These results confirmed previous studies on axillary bud germination and growth, and revealed the important roles of genes involved in plant hormone biosynthesis and signal transduction, aiding in the study of the gene patterns involved in axillary bud germination and growth.
Topics: Plant Growth Regulators; Chrysanthemum; Gene Expression Profiling; Cell Division
PubMed: 38111658
DOI: 10.7717/peerj.16436 -
Neurochemical Research Nov 2020Metabolomic technologies including imaging mass spectrometry (IMS; also called mass spectrometry imaging, MSI, or matrix-assisted laser desorption/ionization-mass... (Review)
Review
Metabolomic technologies including imaging mass spectrometry (IMS; also called mass spectrometry imaging, MSI, or matrix-assisted laser desorption/ionization-mass spectrometry imaging, MALDI MSI) are important methods to evaluate levels of many compounds in brain with high spatial resolution, characterize metabolic phenotypes of brain disorders, and identify disease biomarkers. ATP is central to brain energetics, and reports of its heterogeneous distribution in brain and regional differences in ATP/ADP ratios reported in IMS studies conflict with earlier studies. These discordant data were, therefore, analyzed and compared with biochemical literature that used rigorous methods to preserve labile metabolites. Unequal, very low regional ATP levels and low ATP/ADP ratios are explained by rapid metabolism during postmortem ischemia. A critical aspect of any analysis of brain components is their stability during and after tissue harvest so measured concentrations closely approximate their physiological levels in vivo. Unfortunately, the requirement for inactivation of brain enzymes by freezing or heating is not widely recognized outside the neurochemistry discipline, and procedures that do not prevent postmortem autolysis, including decapitation, brain removal/dissection, and 'snap freezing' are commonly used. Strong emphasis is placed on use of supplementary approaches to calibrate metabolite abundance in units of concentration in IMS studies and comparison of IMS results with biochemical data obtained by different methods to help identify potential artifacts.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Autolysis; Brain; Metabolomics; Specimen Handling; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sugar Phosphates
PubMed: 32949339
DOI: 10.1007/s11064-020-03124-w -
General and Comparative Endocrinology Sep 2021Salusin-β (Sal-β), which originates from preprosalusin, is a multifunctional hormone with a peptide structure. Sal-β exists in the hypothalamus and can stimulate the...
Salusin-β (Sal-β), which originates from preprosalusin, is a multifunctional hormone with a peptide structure. Sal-β exists in the hypothalamus and can stimulate the pituitary gland. The present study was conducted to determine the effects of Sal-β on hormones that play roles in the male reproductive system. Forty male Wistar Albino rats were used in the study. No infusions were performed on the control group, and infusions were applied to the infusion groups (artificial cerebrospinal fluid to the sham group, 2 and 20 nM Sal-β to the experimental group) through intracerebroventricular infusion for 7 days at 10 μl/hour rate. The animals were decapitated after 7 days of infusion; and the hypothalamus, testicles, and blood tissue samples were collected. The gonadotropin-releasing hormone (GnRH) mRNA levels were determined from the hypothalamus tissues by using the Real Time-PCR Method, and the serum luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone levels were determined using the ELISA method. Also, Hematoxylin-Eosin Staining Method was used for histopathological evaluations in the testicle tissues. As a result, Sal-β infusion increased GnRH mRNA levels in hypothalamus tissues (p < 0.05) besides, serum LH, FSH, and testosterone levels of the rats were higher at significant levels following Sal-β infusion compared to the control and sham group (p < 0.05). In the histological examination of the testicle tissues, Sal-β application was found to decrease the seminiferous tubule diameter and germinal epithelial thickness (p < 0.05). This evidence is the first, indicating that Sal-β, which is administered to male rats with central infusion, stimulates hypothalamus and pituitary tissues, and causes increased secretion of male reproductive hormones.
Topics: Animals; Follicle Stimulating Hormone; Gonadotropin-Releasing Hormone; Hypothalamus; Infusions, Intraventricular; Intercellular Signaling Peptides and Proteins; Male; Pituitary Gland; Rats; Rats, Wistar; Testis; Testosterone
PubMed: 34015344
DOI: 10.1016/j.ygcen.2021.113820 -
Avicenna Journal of Phytomedicine 2022Gallic acid (GA) is an organic acid that possesses anti-inflammatory effects as it inhibits the production of metalloproteinases, tissue plasminogen activator, growth...
OBJECTIVE
Gallic acid (GA) is an organic acid that possesses anti-inflammatory effects as it inhibits the production of metalloproteinases, tissue plasminogen activator, growth factors and adhesion molecules. Since formation of abdominal surgery-induced adhesion bands is accompanied by inflammation, angiogenesis and cell proliferation, in the current study, we assessed potential beneficial properties of GA against adhesion bands formation in rats.
MATERIALS AND METHODS
Thirty-six adult male rats were assigned into six groups of six animals. After induction of anesthesia, peritoneal injury was induced using a standard method and animals received either GA (10, 25, 50 and 100 mg/kg), or normal saline, while a group of rats remained intact. Seven days after the surgery, animals were decapitated and samples were collected for pathology evaluations. Also, lipid peroxidation (TBARS) and tumor necrosis factor alpha (TNF-α) levels were determined in serum samples.
RESULTS
Our results showed that GA significantly reduced lipid peroxidation in serum samples but had no effect on TNF-α levels. Furthermore, microscopic and macroscopic injuries reduced significantly in GA-treated animals.
CONCLUSION
Since GA reduced adhesion bands formation at microscopic and macroscopic levels, it could be considered a treatment against adhesion bands formation.
PubMed: 35782766
DOI: 10.22038/AJP.2022.19811 -
Frontiers in Plant Science 2021Temperate deciduous fruit trees survive winter temperatures by entering a dormant phase in their aerial meristematic organs. Release from bud dormancy occurs after chill...
Temperate deciduous fruit trees survive winter temperatures by entering a dormant phase in their aerial meristematic organs. Release from bud dormancy occurs after chill requirements (CR) have been satisfied, whereas bud burst/flowering follows heat requirement (HR) fulfillment. The physiological basis behind these metrics remains elusive. In this study, we are presenting the first multidisciplinary dormancy progression analysis in northern Patagonia, linking (1) forcing/field phenology, (2) bud anatomical development, and (3) soluble sugar (sucrose, glucose, and fructose) dynamics in L. CR and HR were determined for 'Chandler' and 'Franquette,' two walnut cultivars with markedly different CR, in artificial chill/forced heat trials (three seasons) and in-field chill/forced heat tests (five seasons) using excised twigs either with or without apical buds (non-decapitated and decapitated). The soluble sugar dynamics of 'Chandler' (high-performance liquid chromatography) and the anatomical changes of the buds (light microscopy) of the two cultivars were analyzed during endo-ecodormancy progression in one and two seasons, respectively. The CR defined by artificial chill tests proved to be an overestimation compared to the field determinations. Moreover, HR was the main driver in the phenology dynamics, as expected for a high-chill region. 'Chandler' showed an average of 10.3 field chill portions (CP) and 2,163 Growing Degree Hours (GDH°C) less than 'Franquette' for dormancy release and bud burst, respectively. These results were consistent with the transition of the shoot apex from the vegetative to the reproductive phase and the soluble sugar profile. The decrease in sucrose between 15 and 30 days after CR fulfillment could be a reliable biological marker for endodormancy release in walnut, while the increase in fructose and glucose is likely an osmolyte and cellulosic carbon source in pre-sprouting. In addition, we discuss the effect of paradormancy thanks to our apical bud experiment (with or without). Our results improve the current understanding of endo-ecodormancy progression in walnut and provide insightful results for walnut production (i.e., cultivation practices such as pruning) as well as for further application in dormancy modeling, to infer the ideotypes that should be bred for future climate conditions.
PubMed: 35185955
DOI: 10.3389/fpls.2021.803878 -
Anthropologischer Anzeiger; Bericht... Nov 2021In the mid-fourteenth century a cemetery for the burial of the destitute, pilgrims and non-citizens of Gdańsk, Poland, was founded beyond the city walls. During...
In the mid-fourteenth century a cemetery for the burial of the destitute, pilgrims and non-citizens of Gdańsk, Poland, was founded beyond the city walls. During epidemics, the local urban population was buried there in mass graves. Excavations in Gdańsk led to the discovery of four mass graves, the largest of which contained the remains of 759 people who were probably killed by the same plague. In addition to being used as a plague cemetery, this site also served as a burial ground for criminals. Following a single mass execution, 41 decapitated bodies were interred in a mass grave dated to the fifteenth century. The skulls of these individuals were not found during excavation. Skeletal analysis suggests that the executions may have been performed using a mechanical device: a guillotine.
PubMed: 34761797
DOI: 10.1127/anthranz/2021/1462 -
Nutritional Neuroscience Jun 2024Autism is a devastating neurodevelopmental disorder and recent studies showed that omega-3 or astaxanthin might reduce autistic symptoms due to their anti-inflammatory...
Autism is a devastating neurodevelopmental disorder and recent studies showed that omega-3 or astaxanthin might reduce autistic symptoms due to their anti-inflammatory properties. Therefore, we investigated the effects of omega-3 and astaxanthin on the VPA-induced autism model of rats. Female Wistar albino pups ( = 40) were grouped as control, autistic, astaxanthin (2 mg/kg), omega-3 (200 mg/kg), and astaxanthin (2 mg/kg)+omega-3 (200 mg/kg). All groups except the control were prenatally exposed to VPA. Astaxanthin and omega-3 were orally administered from the postnatal day 41 to 68 and behavioral tests were performed between day 69 and 73. The rats were decapitated 24 h after the behavioral tests and hippocampal and prefrontal cytokines and 5-HT levels were analyzed by ELISA. VPA rats have increased grooming behavior while decreased sociability (SI), social preference index (SPI), discrimination index (DI), and prepulse inhibition (PPI) compared to control. Additionally, IL-1β, IL-6, TNF-α, and IFN-γ levels increased while IL-10 and 5-HT levels decreased in both brain regions. Astaxanthin treatment raised SI, SPI, DI, PPI, and prefrontal IL-10 levels. It also raised 5-HT levels and decreased IL-6 levels in both brain regions. Omega-3 and astaxanthin + omega-3 increased the SI, SPI, DI, and PPI and decreased grooming behavior. Moreover, they increased IL-10 and 5-HT levels whereas decreased IL-1β, IL-6, TNF-α, IFN-γ levels in both brain regions. Our results showed that VPA administration mimicked the behavioral and molecular changes of autism in rats. Single and combined administration of astaxanthin and omega-3 improved the autistic-like behavioral and molecular changes in the VPA model of rats.
Topics: Animals; Xanthophylls; Female; Pregnancy; Rats, Wistar; Valproic Acid; Fatty Acids, Omega-3; Autistic Disorder; Disease Models, Animal; Prenatal Exposure Delayed Effects; Behavior, Animal; Hippocampus; Cytokines; Rats; Serotonin; Prefrontal Cortex; Social Behavior
PubMed: 37534957
DOI: 10.1080/1028415X.2023.2239575 -
Pakistan Journal of Pharmaceutical... Jan 2021Present study was designed to monitor the dose dependent effects of lorazepam; a benzodiazepine (CNS depressant). It is the primary drug of choice for treatment of...
Present study was designed to monitor the dose dependent effects of lorazepam; a benzodiazepine (CNS depressant). It is the primary drug of choice for treatment of anxiety and to produce calming effects. However, repeated administration of this lorazepam causes dependence and this might be caused by increased dopaminergic neurotransmission. Besides dopamine, 5-hydroxy tryptamine (5-HT) has also been reported to have pivotal role in the pathophysiology as well as treatment of anxiety and addiction. Repeated administration of lorazepam might involve altered 5-HT metabolism as well. Present study was therefore designed to monitor dose-dependent effects of lorazepam and to select its optimum dose for further experiments and pharmacological interventions. Effects of lorazepam were monitored on food intake, growth rate, activities in familiar and novel environments, light dark box activity, forced swim test and metabolism of dopamine and 5-HT. oral administration of lorazepam was done at the doses of 0mg/kg, 2mg/kg, 4mg/kg and 6mg/kg. Behaviors parameters were monitored following single administration of lorazepam. Rats were decapitated and whole brain samples were collected and stored at -70°C until neurochemical analysis by HPLC-EC. Findings from the present study could be implicated to increased therapeutic utility of lorazepam and related benzodiazepines.
Topics: Animals; Anti-Anxiety Agents; Brain; Dopamine; Eating; Exploratory Behavior; Lorazepam; Male; Motor Activity; Rats; Rats, Wistar; Serotonin
PubMed: 34248013
DOI: No ID Found