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Indian Dermatology Online Journal 2018Schopf-Schulz-Passarge syndrome (SSPS) is a rare type of ectodermal dysplasia that has autosomal recessive inheritance. It is characterized by palmoplantar keratoderma,...
Schopf-Schulz-Passarge syndrome (SSPS) is a rare type of ectodermal dysplasia that has autosomal recessive inheritance. It is characterized by palmoplantar keratoderma, hypodontia, hypotrichosis, nail dystrophy, and multiple periocular and eyelid apocrine hidrocystomas. A 36-year-old male presented with multiple eyelid and periocular apocrine hidrocystomas, ichthyosis, palmoplantar keratoderma, hypodontia, nail dystrophy, and thin scalp hair. Skin biopsy from a periocular lesion revealed cyst lined with smooth, thin epithelium, and few areas revealed foci of decapitation secretion consistent with apocrine hidrocystoma. The patient was diagnosed with SSPS.
PubMed: 30505790
DOI: 10.4103/idoj.IDOJ_26_18 -
Nature Communications Aug 2021The sperm head-to-tail coupling apparatus (HTCA) ensures sperm head-tail integrity while defective HTCA causes acephalic spermatozoa, rendering males infertile. Here, we...
The sperm head-to-tail coupling apparatus (HTCA) ensures sperm head-tail integrity while defective HTCA causes acephalic spermatozoa, rendering males infertile. Here, we show that CENTLEIN is indispensable for HTCA integrity and function, and that inactivation of CENTLEIN in mice leads to sperm decapitation and male sterility. We demonstrate that CENTLEIN directly interacts with both SUN5 and PMFBP1, two proteins localized in the HTCA and related with acephalic spermatozoa syndrome. We find that the absence of Centlein sets SUN5 and PMFBP1 apart, the former close to the sperm head and the latter in the decapitated tail. We show that lack of Sun5 results in CENTLEIN and PMFBP1 left in the decapitated tail, while disruption of Pmfbp1 results in SUN5 and CENTLEIN left on the detached sperm head. These results demonstrate that CENTLEIN cooperating with SUN5 and PMFBP1 participates in the HTCA assembly and integration of sperm head to the tail, indicating that impairments of CENTLEIN might be associated with acephalic spermatozoa syndrome in humans.
Topics: Animals; Cell Cycle Proteins; Cells, Cultured; Cytoskeletal Proteins; HEK293 Cells; Humans; Infertility, Male; Male; Membrane Proteins; Mice, Inbred C57BL; Mice, Inbred DBA; Mice, Knockout; Mutation; Protein Binding; Sperm Head; Sperm Tail; Spermatozoa; Teratozoospermia; Mice
PubMed: 34389728
DOI: 10.1038/s41467-021-25227-w -
Genes Nov 2022Petunia is one of the world's most important flowers, and its branch development has long been a source of discussion. MYB transcription factors have been identified as...
Petunia is one of the world's most important flowers, and its branch development has long been a source of discussion. MYB transcription factors have been identified as important plant branching regulators. In this study, 113 R2R3-MYB genes were identified from the petunia genome. genes, closely related to , were expressed at greater levels in axillary buds and roots. Decapitation and 6-BA did not regulate the expression of . PhMYB37 was localized in the nucleus. Heterologous overexpression of promoted shoot branching in transgenic Arabidopsis while silencing of inhibited shoot branching. These results suggest that plays a critical and positive role in petunia shoot branching.
Topics: Petunia; Gene Expression Regulation, Plant; Plant Proteins; Arabidopsis; Flowers
PubMed: 36360301
DOI: 10.3390/genes13112064 -
Ecotoxicology and Environmental Safety Jun 2021Decapitation and root cutting can influence plant physiological features, such as height, dry weight, and transpiration rate, which partly determine the success of...
Decapitation and root cutting can influence plant physiological features, such as height, dry weight, and transpiration rate, which partly determine the success of phytoremediation. In this study, the effects of three root cutting intensities (10%, 25%, and 33%), decapitation, and their combination on the phytoremediation efficiency of Celosia argentea were evaluated. Decapitation increased the biomass yield of C. argentea roots and leaves and significantly improved the species' Cd decontamination ability. Root cutting, especially 33% cutting treatment, decreased the root dry weight. The 10% and 25% root cutting treatments increased the leaf biomass yield by 58.6% and 41.4%, respectively, compared with the untreated control, even compensating for the loss of roots, but 33% root cutting decreased the leaf dry weight. Low and moderate root cutting intensity (10% and 25%) increased the leaf Cd content by 33.4% and 24.9%, respectively, and was associated with improved transpiration rate. The highest root and leaf dry weights were observed for the combination of decapitation and 10% root cutting, which increased the biomass yield of underground and aerial parts by 109.9% and 286.2%, respectively. In addition, decapitation offset the negative effects of 33% root cutting on plant growth, indicated by the higher dry weight relative to the control. Decapitated C. argentea accumulated 11.0, 7.5, and 0.7 times more Cd with the 10%, 25%, and 33% root cutting treatments, respectively, compared with the control. The combination of root cutting and decapitation was a practicable and economical method of enhancing the Cd decontamination capacity of C. argentea.
Topics: Biodegradation, Environmental; Biomass; Celosia; Decapitation; Plant Roots; Plants; Soil; Soil Pollutants
PubMed: 33756287
DOI: 10.1016/j.ecoenv.2021.112162 -
BMC Plant Biology Aug 2022Aerial bulbils are important vegetative reproductive organs in Lilium. They are often perpetually dormant in most Lilium species, and little is known about the induction...
Aerial bulbils are important vegetative reproductive organs in Lilium. They are often perpetually dormant in most Lilium species, and little is known about the induction of these vegetative structures. The world-famous Oriental hybrid lily cultivar 'Sorbonne', which blooms naturally devoid of aerial bulbils, is known for its lovely appearance and sweet fragrance. We found that decapitation stimulated the outgrowth of aerial bulbils at lower stems (LSs) and then application of low and high concentrations of IAA promoted aerial bulbils emergence around the wound at upper stems (USs) of 'Sorbonne'. However, the genetic basis of aerial bulbil induction is still unclear. Herein, 'Sorbonne' transcriptome has been sequenced for the first time using the combination of third-generation long-read and next-generation short-read technology. A total of 46,557 high-quality non-redundant full-length transcripts were generated. Transcriptomic profiling was performed on seven tissues and stems with treatments of decapitation and application of low and high concentrations of IAA, respectively. Functional annotation of 1918 DEGs within stem samples of different treatments showed that hormone signaling, sugar metabolism and wound-induced genes were crucial to bulbils outgrowth. The expression pattern of auxin-, shoot branching hormone-, plant defense hormone- and wound-inducing-related genes indicated their crucial roles in bulbil induction. Then we established five hormone- and wounding-regulated co-expression modules and identified some candidate transcriptional factors, such as MYB, bZIP, and bHLH, that may function in inducing bulbils. High connectivity was observed among hormone signaling genes, wound-induced genes, and some transcriptional factors, suggesting wound- and hormone-invoked signals exhibit extensive cross-talk and regulate bulbil initiation-associated genes via multilayered regulatory cascades. We propose that the induction of aerial bulbils at LSs after decapitation can be explained as the release of apical dominance. In contrast, the induction of aerial bulbils at the cut surface of USs after IAA application occurs via a process similar to callus formation. This study provides abundant candidate genes that will deepen our understanding of the regulation of bulbil outgrowth, paving the way for further molecular breeding of lily.
Topics: Decapitation; Gene Expression Profiling; Gene Expression Regulation, Plant; Hormones; Lilium; Plant Growth Regulators; Transcriptome
PubMed: 36030206
DOI: 10.1186/s12870-022-03801-8 -
Surgical Neurology International 2016The HEAVEN head transplant initiative needs human data concerning the acute restoration of motor transmission after application of fusogens to the severed cord in man....
The HEAVEN head transplant initiative needs human data concerning the acute restoration of motor transmission after application of fusogens to the severed cord in man. Data from two centuries ago prove that a fresh cadaver, after hanging or decapitation, can be mobilized by electrical stimulation for up to 3 hours. By administering spinal cord stimulation by applied paddles to the cord or transcranial magnetic stimulation to M1 and recording motor evoked potentials, it should be possible to test fusogens in fresh cadavers. Delayed neuronal death might be the neuropathological reason.
PubMed: 27656323
DOI: 10.4103/2152-7806.190472 -
The Journal of Experimental Biology Sep 2022Certain animal species utilize electric fields for communication, hunting and spatial orientation. Freshwater planarians move toward the cathode in a static electric...
Certain animal species utilize electric fields for communication, hunting and spatial orientation. Freshwater planarians move toward the cathode in a static electric field (cathodic electrotaxis). This planarian behavior was first described by Raymond Pearl more than a century ago. However, planarian electrotaxis has received little attention since, and the underlying mechanisms and evolutionary significance remain unknown. To close this knowledge gap, we developed an apparatus and scoring metrics for automated quantitative and mechanistic studies of planarian behavior upon exposure to a static electric field. Using this automated setup, we characterized electrotaxis in the planarian Dugesia japonica and found that this species responds to voltage instead of current, in contrast to results from previous studies using other planarian species. Surprisingly, we found differences in electrotaxis ability between small (shorter) and large (longer) planarians. To determine the cause of these differences, we took advantage of the regenerative abilities of planarians and compared electrotaxis in head, tail and trunk fragments of various lengths. We found that tail and trunk fragments electrotaxed, whereas head fragments did not, regardless of size. Based on these data, we hypothesized that signals from the head may interfere with electrotaxis when the head area/body area reached a critical threshold. In support of this hypothesis, we found that (1) smaller intact planarians that cannot electrotax have a relatively larger head-to-body-ratio than large planarians that can electrotax, and (2) the electrotaxis behavior of cut head fragments was negatively correlated with the head-to-body ratio of the fragments. Moreover, we could restore cathodic electrotaxis in head fragments via decapitation, directly demonstrating inhibition of electrotaxis by the head.
Topics: Animals; Biological Evolution; Planarians
PubMed: 35924486
DOI: 10.1242/jeb.243972 -
Evolutionary Psychology : An... Oct 2021At the beginning of our era, after a battle on the Ionian Sea, Antony and Cleopatra took their own lives in Egypt, and Augustus was made an by his senators Roman...
At the beginning of our era, after a battle on the Ionian Sea, Antony and Cleopatra took their own lives in Egypt, and Augustus was made an by his senators Roman emperors had sexual access to those senators' daughters and wives, and to thousands of slaves. But they ran governments with help from their castrated civil servants. And they enforced an Imperial Cult: subjects made sacrifices to the emperor's or procreative spirit; or they got disemboweled by wild animals, or decapitated. Then Constantine moved off from the Tiber to the Bosporus, and Europe was ruled over by a few. Lords covered the countryside with bastards, but passed on estates on to their oldest sons. Daughters and younger sons were put away in the Church, where some became parents, but most were reproductively suppressed: they were or or without a husband, and ἄγαμος or or without a wife. Heretics who objected got burned at the stake. Then the Crusaders expanded Europe to the East, and Columbus went off to the West, and politics, sex and religion became more democratic. Power was more widely distributed; more men and women had families if they wanted them, and monasteries emptied out. The Reformation followed the Roman Church, which had followed the Imperial Cult.
Topics: Catholicism; Christianity; Europe; Female; Humans; Male; Politics; Religion; Sexual Behavior
PubMed: 34939448
DOI: 10.1177/14747049211066795 -
Journal of Cell Science Oct 2022Cilia sense and transduce sensory stimuli, homeostatic cues and developmental signals by orchestrating signaling reactions. Extracellular vesicles (EVs) that bud from...
Cilia sense and transduce sensory stimuli, homeostatic cues and developmental signals by orchestrating signaling reactions. Extracellular vesicles (EVs) that bud from the ciliary membrane have well-studied roles in the disposal of excess ciliary material, most dramatically exemplified by the shedding of micrometer-sized blocks by photoreceptors. Shedding of EVs by cilia also affords cells with a powerful means to shorten cilia. Finally, cilium-derived EVs may enable cell-cell communication in a variety of organisms, ranging from single-cell parasites and algae to nematodes and vertebrates. Mechanistic understanding of EV shedding by cilia is an active area of study, and future progress may open the door to testing the function of ciliary EV shedding in physiological contexts. In this Cell Science at a Glance and the accompanying poster, we discuss the molecular mechanisms that drive the shedding of ciliary material into the extracellular space, the consequences of shedding for the donor cell and the possible roles that ciliary EVs may have in cell non-autonomous contexts.
Topics: Animals; Cell Communication; Cilia; Cytoplasmic Vesicles; Extracellular Vesicles; Signal Transduction
PubMed: 36222105
DOI: 10.1242/jcs.246553