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Folia Medica Feb 2021The use of central venous catheters for hemodialysis continues to grow worldwide, despite the efforts of many specialists. Patients with end-stage renal disease have... (Review)
Review
The use of central venous catheters for hemodialysis continues to grow worldwide, despite the efforts of many specialists. Patients with end-stage renal disease have impaired immunity, which is why infections are the most common complication seen in them. It worsens their quality of life and is a major cause of high morbidity and mortality, especially in hemodialysis patients.We report two cases of catheter-related bloodstream infection in hemodialysis patients caused by Ochrobactrum anthropi, which are the first reported cases in Bulgaria and present a brief literature review of the known facts.
Topics: Aged; Bacteremia; Bulgaria; Catheter-Related Infections; Catheters; Gram-Negative Bacterial Infections; Humans; Kidney Failure, Chronic; Male; Middle Aged; Ochrobactrum anthropi; Renal Dialysis
PubMed: 33650409
DOI: 10.3897/folmed.63.e53131 -
Cornea May 2021To report a case of Ochrobactrum anthropi keratitis in an eye with a Boston type 1 keratoprosthesis.
PURPOSE
To report a case of Ochrobactrum anthropi keratitis in an eye with a Boston type 1 keratoprosthesis.
METHODS
This is a case report and review of the literature.
RESULTS
A 78-year-old man with a history of implantation of a Boston type 1 keratoprosthesis in the left eye presented for a routine follow-up with no acute complaints. In the left eye, visual acuity was 20/60 and slit-lamp examination revealed a 1.5-mm inferotemporal corneal infiltrate adjacent to the optic stem. Corneal cultures grew abundant O. anthropi. After 7 weeks of topical antimicrobial therapy and placement of a temporary tarsorrhaphy, the keratitis resolved.
CONCLUSIONS
Ochrobactrum anthropi is an organism associated with indwelling medical devices and can be pathogenic in eyes with implanted keratoprostheses.
Topics: Aged; Anti-Bacterial Agents; Artificial Organs; Ceftazidime; Cornea; Corneal Ulcer; Drug Therapy, Combination; Eye Infections, Bacterial; Gram-Negative Bacterial Infections; Humans; Male; Moxifloxacin; Ochrobactrum anthropi; Prostheses and Implants; Slit Lamp Microscopy; Visual Acuity
PubMed: 33252382
DOI: 10.1097/ICO.0000000000002571 -
Clinical Case Reports Jun 2024(. ), a rare opportunistic pathogen, caused sepsis in a malnourished 15-month-old African child. Early detection and appropriate antibiotics led to full recovery,...
KEY CLINICAL MESSAGE
(. ), a rare opportunistic pathogen, caused sepsis in a malnourished 15-month-old African child. Early detection and appropriate antibiotics led to full recovery, highlighting the importance of robust surveillance for emerging pathogens in vulnerable populations.
ABSTRACT
While rarely causing infections, . , a non-fermenting, obligately aerobic, flagellated gram-negative bacillus, demonstrates oxidase positivity and indole negativity. Traditionally, spp is considered a low threat due to its environmental abundance and mild virulence. It is, however, a multidrug-resistant bacteria known for causing opportunistic infections in humans. . is typically associated with catheter-related bloodstream infections. The first documented case was in 1998; most cases have been reported in developed countries. We present a case of . sepsis in a malnourished child in sub-Saharan Africa. We report a case involving a 15-month-old African female who presented with symptoms and signs of protein-energy malnutrition and sepsis. The blood culture revealed .. We treated the child with the empirical first-line antibiotics per the national guidelines, intravenous ampicillin and gentamicin for a week, and the child fully recovered. This report describes a rare case of . sepsis with malnutrition in an African female child. . is an emerging pathogen causing opportunistic infections in both immunocompetent and immunocompromised patients. We report that early bacterial detection, appropriate antibiotic susceptibility and antimicrobial management based on local antibiogram data may be essential for excellent patient outcomes. Additionally, we recommend more robust surveillance to detect such rare emerging pathogens.
PubMed: 38845804
DOI: 10.1002/ccr3.9042 -
Folia Microbiologica Nov 2019The aim of this work was to compare production of endotoxin and to determine susceptibility to antibiotics in two groups of specimens-wild-type strains Ochrobactrum... (Comparative Study)
Comparative Study
The aim of this work was to compare production of endotoxin and to determine susceptibility to antibiotics in two groups of specimens-wild-type strains Ochrobactrum anthropi isolated from the environment and the strains isolated from patients with cystic fibrosis. The determination of the endotoxin produced by the test strains was carried on by using a limulus amebocyte lysate test (LAL test). Determination of ATB sensitivity was accomplished by means of a broth dilution method in a microtiter plate (MIC). No significant difference was found between the group of ochrobacters isolated from the environment and the group of ochrobacters isolated from cystic fibrosis patients. Antibiotic sensitivity testing has indicated that the resistance to tigecycline, trimethoprim/sulfamethoxazole, and gentamicin was slightly higher in strains isolated from cystic fibrosis patients in comparison with strains isolated from the environment. In general, most of the test strains were sensitive to most of the antibiotics tested. Significant resistance has been demonstrated for cefotaxime. Resistance was also found for gentamicin in strains number 4 and 7. The MIC was equal to the breakpoint for this antibiotic (8000 mg/L).
Topics: Adolescent; Anti-Bacterial Agents; Child; Cystic Fibrosis; Drug Resistance, Bacterial; Endotoxins; Environmental Microbiology; Gram-Negative Bacterial Infections; Humans; Limulus Test; Microbial Sensitivity Tests; Ochrobactrum anthropi
PubMed: 30919306
DOI: 10.1007/s12223-019-00700-8 -
The Science of the Total Environment Feb 2021Previous studies have provided evidence that bioremediation deals a novel approach to graffiti removal, thereby overcoming well-known limitations of current cleaning...
Previous studies have provided evidence that bioremediation deals a novel approach to graffiti removal, thereby overcoming well-known limitations of current cleaning methods. In the present study eight bacteria aerobic, mesophilic and culturable from the American ATCC and the German DSMZ collections of microorganisms, some isolated from car paint waste, colored deposits in a pulp dryer and wastewater from dye works, were tested in the removal of silver and black graffiti spray paints using immersion strategies with glass slides. Absorbance at 600 nm and live/dead assays were performed to estimate bacterial density and activity in all samples. Also, pH and dissolved organic carbon (DOC) and inorganic carbon (DIC) measurements in the liquid media were made, as well as, thickness, colorimetric and infrared (FTIR) spectroscopy measurements in graffiti paint layers were used to evaluate the presence of the selected bacteria in the samples and the graffiti bioremoval capacity of bacteria. Data demonstrated that of the eight bacteria studied, Enterobacter aerogenes, Comamonas sp. and a mixture of Bacillus sp., Delftia lacustris, Sphingobacterium caeni, and Ochrobactrum anthropi were the most promising for bioremoval of graffiti. According to significant changes in FTIR spectra, indicating an alteration of the paint polymeric structure, coupled with the presence of a consistent quantity of live bacteria in the medium as well as a significant increase of DIC (a measure of metabolic activity) and a change in paint color.
Topics: Bacteria; Biodegradation, Environmental; Delftia; Sphingobacterium
PubMed: 33280882
DOI: 10.1016/j.scitotenv.2020.144075 -
Microorganisms Jun 2024() is found in water, soil, plants and animals. Even though it has low virulence, it has increasingly been found to cause a number of infectious diseases in people with...
() is found in water, soil, plants and animals. Even though it has low virulence, it has increasingly been found to cause a number of infectious diseases in people with low immunity. The identification of mainly uses biochemical methods, such as the API 20NE or Vitek-2. The typing studies of have mainly utilized PFGE, rep-PCR, AFLP, 16s rDNA sequencing, RecA-PCR RFLP, and MALDI-TOF MS. This study aims to evaluate the polymorphisms of variable-number tandem-repeats (VNTRs) within genomic DNA of strains. The tandem repeats (TRs) in genomic DNA are discovered using Tandem Repeat Finder software (version 4.09). Twelve different VNTRs are designated and assigned to the nomenclature. The primers for PCR of 12 loci are designed. The PCR product size is converted to the number of tandem repeats in every locus. The relatedness of 65 strains from geographically different countries are analyzed by means of 12-variable-number tandem-repeat analysis(MLVA-12). A total of 51 different genotypes are found in 65 strains. These strains, which were collected from the same environmental samples, hospitals, and countries, are clustered within the same or closely genotypes. The MLVA-12 assay has a good discriminatory power for species determination, typing of , and inferring the origin of bacteria.
PubMed: 38930593
DOI: 10.3390/microorganisms12061211 -
Journal of Biological Engineering Feb 2023Aromatic primary amines (APAs) are key intermediates in the chemical industry with numerous applications. Efficient and mild biocatalytic synthesis is an excellent...
BACKGROUND
Aromatic primary amines (APAs) are key intermediates in the chemical industry with numerous applications. Efficient and mild biocatalytic synthesis is an excellent complement to traditional chemical synthesis. Our lab previously reported a whole-cell catalytic system for the synthesis of APAs catalyzed by carboxylic acid reductase from Neurospora crassa (ncCAR) and ω-transaminase from Ochrobactrum anthropi (OATA). However, the accumulation of toxic intermediates (aromatic aldehydes) during biocatalytic synthesis affected yields of APAs due to metabolic imbalance.
RESULTS
In this work, the biocatalytic synthesis of APAs (taking cinnamylamine as an example) was metabolically regulated by the overexpression or knockout of five native global transcription factors (TFs), the overexpression of eight native resistance genes, and optimization of promoters. Transcriptome analysis showed that knockout of the TF arcA increased the fluxes of NADPH and ATP in E. coli, while the rate of pyruvate metabolism was accelerated. In addition, the genes related to stress and detoxification were upregulated with the overexpression of resistance gene marA, which reduced the NADPH level in E. coli. Then, the expression level of soluble OATA increased by promoter optimization. Overall, arcA and marA could regulate the catalytic rate of NADPH- dependent ncCAR, while arcA and optimized promoter could regulate the catalytic rate of OATA. Lastly, the cinnamylamine yield of the best metabolically engineered strain S020 was increased to 90% (9 mM, 1.2 g/L), and the accumulation of cinnamaldehyde was below 0.9 mM. This work reported the highest production of cinnamylamine by biocatalytic synthesis.
CONCLUSION
This regulatory process provides a common strategy for regulating the biocatalytic synthesis of other APAs. Being entirely biocatalytic, our one-pot procedure provides considerable advantages in terms of environmental and safety impacts over reported chemical methods.
PubMed: 36823535
DOI: 10.1186/s13036-023-00334-y -
BMC Research Notes May 2020Di-2-ethylhexyl phthalate (DEHP) pollution is one of the major environmental concerns all over the world. This research aimed at studying the biodegradation kinetics of...
OBJECTIVE
Di-2-ethylhexyl phthalate (DEHP) pollution is one of the major environmental concerns all over the world. This research aimed at studying the biodegradation kinetics of DEHP by a newly isolated bacterial strain. Water and sediment samples were collected from Wuhan South Lake and potent bacterial isolates were screened for DEHP degradation, characterized by biochemical, physiological, morphological and 16S rDNA gene sequencing, and optimized under suitable pH, temperature, NaCl and DEHP concentrations. DEHP and its metabolites were quantified by High Performance Liquid Chromatography and their degradation kinetics were studied.
RESULTS
The newly isolated bacterium was identified as Ochrobactrum anthropi strain L1-W with 99.63% similarity to Ochrobactrum anthropi ATCC 49188. It was capable of utilizing DEHP as the carbon source. The optimum growth temperature, pH, DEHP and NaCl concentration for the strain L1-W were 30 °C, 6, 400 mg/L and 10 g/L respectively. Strain L1-W was capable of degrading almost all (98.7%) of DEHP when the initial concentration was 200 mg/L within a period of 72 h. Besides, it was also found capable of degrading five other phthalates, thus making it a possible candidate for bioremediation of phthalates in the environmental settings.
Topics: Biodegradation, Environmental; China; Chromatography, High Pressure Liquid; Diethylhexyl Phthalate; Humans; Hydrogen-Ion Concentration; Kinetics; Ochrobactrum anthropi; Phylogeny; RNA, Ribosomal, 16S; Soil; Temperature
PubMed: 32448295
DOI: 10.1186/s13104-020-05096-0 -
Scientific Reports Apr 2023In a survey conducted during the period of March-May 2019 in nurseries, warehouses, and shops at three governorates (Alexandria, El-Behera, and Giza governorates,...
In a survey conducted during the period of March-May 2019 in nurseries, warehouses, and shops at three governorates (Alexandria, El-Behera, and Giza governorates, Egypt), symptoms of root rot, basal stem rot, and wilt disease complex were observed in the lucky bamboo (Dracaena sanderiana hort. ex. Mast.). The highest disease infection percentage was found in lucky bamboo collected from Alexandria City (47.67%), while the highest disease severity was in lucky bamboo collected from El-Behera Governorate (35.19%). Rhizoctonia solani, Fusarium oxysporum, F. solani, Aspergillus niger, and Alternaria alternate were isolated and identified in the infected lucky bamboo samples. R. solani isolates were the most dominant among the recovered fungal species with a percentage of 80.89% of the total isolates (246). Pathogenicity tests showed that R. solani was the most pathogen with 100% disease infection and 76.67% disease severity. Molecular identification characterized R. solani isolate as R. solani AUMC 15120, MZ723906. Meanwhile, four biological control agents (bioagents) were isolated from the healthy lucky bamboo samples and identified based on cultural, morphological, microscopic characteristics, and the molecular phylogenetic analysis as Clonostachys rosea AUMC 15121, OL461708; Bacillus circulans TAG1, MW441316; B. siamensis TAP1, MW441318 and Ochrobactrum anthropi TAM1, MW441317. The four bioagents showed potential inhibition of R. solani in vitro as well as in vivo on lucky bamboo plants in vase treatments compared to the untreated inoculated control as well as certain fungicides and biocides used (Moncut, Rizolex-T, Topsin-M, Bio-Zeid, and Bio-Arc). The bioagent O. anthropi showed the highest inhibition growth (85.11%) of the in vitro R. solani colony, which was not significantly different from the biocide Bio-Arc (83.78%). However, C. rosea, B. siamensis and B. circulans showed inhibition values of 65.33, 64.44, and 60.44%, respectively. On the other hand, the biocide Bio-Zeid showed less inhibitory effect (43.11%), while the lowest growth inhibition was recorded by Rizolex-T (34.22%) and Topsin-M (28.67%). Furthermore, the in vivo experiment supported the in vitro results for the most effective treatments, where all the treatments significantly decreased the percentage of infection and disease severity compared to the inoculated untreated control. Additionally, the bioagent O. anthropi showed the highest effect, i.e., the lowest disease incidence and disease severity being 13.33% and 10%, compared to 100% and 75%, respectively, in the untreated inoculated control. This was not significantly different from the fungicide Moncut (13.33% and 21%) and from the bioagent C. rosea (20% and 15%) treatments for both parameters, respectively. In conclusion, the bioagents O. anthropi MW441317 at 1 × 10 CFU/ml as well as C. rosea AUMC15121 at 1 × 10/ml proved to be efficient to control R. solani causing root rot, and basal stem rot on lucky bamboo, compared to fungicide Moncut and can be used for disease management without the negative impact of the chemical control. Furthermore, this is the first report of the isolation and identification of Rhizoctonia solani, a pathogenic fungus, and four biocontrol agents (Bacillus circulans, B. siamensis, Ochrobactrum anthropi and Clonostachys rosea) associated with the healthy lucky bamboo plants.
Topics: Dracaena; Phylogeny; Fungicides, Industrial
PubMed: 37095150
DOI: 10.1038/s41598-023-33628-8 -
Journal of Orthopaedic Case Reports Mar 2022Ochrobactrum anthropi is an unusual low virulence emerging pathogen that rarely causes orthopedic infection and its clinical picture is not well described. It usually...
INTRODUCTION
Ochrobactrum anthropi is an unusual low virulence emerging pathogen that rarely causes orthopedic infection and its clinical picture is not well described. It usually causes infection in immunocompromised hosts with indwelling catheters or foreign bodies, such as the central venous catheters.
CASE REPORT
We reported a case of O. anthropi pyomyositis in a 22-year-old immunocompetent male patient not on any invasive procedure presented with raised temperature, left shoulder pain, and restriction of movements. Diagnosis was confirmed with the help of MRI and biopsy. He was successfully managed with surgical debridement and appropriate antibiotics.
CONCLUSION
Our case highlights the ability of O. anthropi to cause pyomyositis in immunocompetent individuals and its relevance in the field of orthopaedic infection.
PubMed: 36199917
DOI: 10.13107/jocr.2022.v12.i03.2700