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Gene May 2022The Tibetan Plateau niche provides unprecedented opportunities to find microbes that are functional and commercial significance. The present study investigated the...
The Tibetan Plateau niche provides unprecedented opportunities to find microbes that are functional and commercial significance. The present study investigated the physiological and genomic characteristics of Planococcus halotolerans Y50 that was isolated from a petroleum-contaminated soil sample from the Qinghai-Tibet Plateau, and it displayed psychrotolerant, antiradiation, and oil-degraded characteristics. Whole genome sequencing indicated that strain Y50 has a 3.52 Mb genome and 44.7% G + C content, and it possesses 3377 CDSs. The presence of a wide range of UV damage repair genes uvrX and uvsE, DNA repair genes radA and recN, superoxide dismutase, peroxiredoxin and dioxygenase genes provided the genomic basis for the adaptation of the plateau environment polluted by petroleum. Related experiments also verified that the Y50 strain could degrade n-alkanes from C-C, and approximately 30% of the total petroleum at 25 °C within 7 days. Meanwhile, strain Y50 could withstand 5 × 10 J/m UVC and 10 KGy gamma ray radiation, and it had strong antioxidant and high radical scavengers for superoxide anion, hydroxyl radical and DPPH. In addition, pan-genome analysis and horizontal gene transfers revealed that strains with different niches have obtained various genes through horizontal gene transfer in the process of evolution, and the more similar their geographical locations, the more similar their members are genetically and ecologically. In conclusion, P. halotolerans Y50 possesses high potential of applications in the bioremediation of alpine hydrocarbons contaminated environment.
Topics: Base Composition; Biodegradation, Environmental; Genome Size; Genome, Bacterial; Petroleum; Phylogeny; Planococcaceae; Soil Microbiology; Tibet; Whole Genome Sequencing
PubMed: 35240255
DOI: 10.1016/j.gene.2022.146368 -
Biology Oct 2023The Barents Sea is one of the most rapidly changing Arctic regions, with an unprecedented sea ice decline and increase in water temperature and salinity. We have studied...
The Barents Sea is one of the most rapidly changing Arctic regions, with an unprecedented sea ice decline and increase in water temperature and salinity. We have studied the diversity of prokaryotic communities using 16S metabarcoding in the western and northeastern parts of the Barents Sea along the Kola Section and the section from Novaya Zemlya to Franz Joseph Land. The hypothesis-independent clustering method revealed the existence of two distinct types of communities. The most common prokaryotic taxa were shared between two types of communities, but their relative abundance was different. It was found that the geographic location of the sampling sites explained more than 30% of the difference between communities, while no statistically significant correlation between environmental parameters and community composition was found. The representatives of the , and genera were dominant in samples from both types of communities. The first type of community was also dominated by members of , , and an unclassified representative of the family. The second type of community also had a significant proportion of , SAR92, SAR11 Clade I, NS9, and SUP05 representatives. The origin of these communities can be explained by the influence of environmental factors or by the different origins of water masses. This research highlights the importance of studying biogeographic patterns in the Barents Sea in comparison with those in the North Atlantic and Arctic Ocean prokaryote communities.
PubMed: 37887020
DOI: 10.3390/biology12101310 -
International Journal of Biological... Jan 2022β-carotene is a natural compound with immense healthcare benefits. To overcome insolubility and lack of stability which restricts its application, in this study,... (Comparative Study)
Comparative Study
Comparative performance evaluation of chitosan based polymeric microspheres and nanoparticles as delivery system for bacterial β-carotene derived from Planococcus sp. TRC1.
β-carotene is a natural compound with immense healthcare benefits. To overcome insolubility and lack of stability which restricts its application, in this study, β-carotene from Planococcus sp. TRC1 was entrapped into formulations of chitosan‑sodium alginate microspheres (MF1, MF2 and MF3) and chitosan nanoparticles (NF1, NF2 and NF3). The maximum entrapment efficiency (%) and loading capacity (%) were 80.6 ± 4.28 and 26 ± 3.05 (MF2) and 92.1 ± 3.44 and 41.86 ± 4.65 (NF2) respectively. Korsmeyer-Peppas model showed best fit with release, revealing non-Fickian diffusion. Thermal and UV treatment exhibited higher activation energy (kJ/mol), 17.76 and 15.57 (MF2) and 37.03 and 19.33 (NF2) compared to free β-carotene (3.7 and 3.9), uncovering enhanced stability. MF2 and NF2 revealed swelling index (%) 721 ± 1.7 and 18.1 ± 1.5 (pH 6.8) and particle size 69.5 ± 3.2 μm and 92 ± 2.5 nm respectively. FESEM, FT-IR, XRD and DSC depicted spherical morphology, intactness of functional groups and masking of crystallinity. The IC (μg ml) values for antioxidant and anticancer (A-549) activities were 33.1 ± 1.7, 45.1 ± 2.8, 39.3 ± 2.9 and 31.3 ± 1.7, 27.9 ± 2.4, 25.3 ± 2.2 for β-carotene, MF2 and NF2 respectively with no significant cytotoxicity on HEK-293 cells and RBCs (p > 0.05). This comparative study of microspheres and nanoparticles may allow the diverse applications of an unconventional bacterial β-carotene with promising stability and efficacies.
Topics: Alginates; Chemistry, Pharmaceutical; Chitosan; Diffusion; Drug Carriers; Drug Compounding; Drug Delivery Systems; HEK293 Cells; Humans; Microspheres; Nanoparticles; Particle Size; Planococcaceae; Spectroscopy, Fourier Transform Infrared; beta Carotene
PubMed: 34863970
DOI: 10.1016/j.ijbiomac.2021.11.167 -
Scientific Reports Dec 2023This research examines the biological treatment of undiluted vegetable waste digestate conducted in a bubble column photobioreactor. Initially, the bioreactor containing...
This research examines the biological treatment of undiluted vegetable waste digestate conducted in a bubble column photobioreactor. Initially, the bioreactor containing 3N-BBM medium was inoculated with Microglena sp., Tetradesmus obliquus, and Desmodesmus subspicatus mixture with a density of 1.0 × 10 cells/mL and the consortium was cultivated for 30 days. Then, the bioreactor was semi-continuously fed with liquid digestate with hydraulic retention time (HRT) of 30 days, and the treatment process was continued for the next 15 weeks. The change in the microalgal and cyanobacterial species domination was measured in regular intervals using cell counting with droplet method on a microscope slide. At the end of the experiment, Desmonostoc sp. cyanobacteria (identified with 16S ribosomal RNA genetical analysis) as well as Tetradesmus obliquus green algae along with Rhodanobacteraceae and Planococcaceae bacteria (determined with V3-V4 16sRNA metagenomic studies) dominated the microbial community in the photobioreactor. The experiment demonstrated high treatment efficiency, since nitrogen and soluble COD were removed by 89.3 ± 0.5% and 91.2 ± 1.6%, respectively, whereas for phosphates, 72.8 ± 2.1% removal rate was achieved.
Topics: Photobioreactors; Waste Disposal, Fluid; Microalgae; Vegetables; Anaerobiosis; Cyanobacteria; Nitrogen; Chlorophyceae; Biomass
PubMed: 38114556
DOI: 10.1038/s41598-023-50173-6 -
Current Microbiology Dec 2021Two novel bacteria species designated Marseille-Q1000 and Marseille-Q0999 were isolated from urine samples of patients in Sokoto, Northwest-Nigeria. They were...
Two novel bacteria species designated Marseille-Q1000 and Marseille-Q0999 were isolated from urine samples of patients in Sokoto, Northwest-Nigeria. They were Gram-positive bacteria and belong to two different genera, Bhargavaea and Dietzia. The genome size and G + C content of Marseille-Q1000 and Marseille-Q0999 were 3.07 and 3.51 Mbp with 53.8 and 71.0 mol% G + C content, respectively. The strains exhibited unique phenotypic and genomic features that are substantially different from previously known bacterial species with standing in nomenclature. On the basis of the phenotypic, phylogenetic and genomic characteristics, strains Marseille-Q0999 (= CSURQ0999 = DSM 112394) and Marseille-Q1000 (= CSURQ1000 = DSM 112384) were proposed as the type strains of Bhargavaea massiliensis sp. nov., and Dietzia massiliensis sp. nov., respectively.
Topics: DNA, Bacterial; Humans; Nigeria; Phylogeny; Planococcaceae; RNA, Ribosomal, 16S
PubMed: 34905116
DOI: 10.1007/s00284-021-02721-4 -
Environmental Science and Pollution... Aug 2023The microbial reduction of Cr(VI) to Cr(III) is widely applied, but most studies ignored the stability of reduction products. In this study, the Cr(VI)-reducing...
The microbial reduction of Cr(VI) to Cr(III) is widely applied, but most studies ignored the stability of reduction products. In this study, the Cr(VI)-reducing bacterium of Sporosarcina saromensis combined with microbially induced carbonate precipitation (MICP) was used to explore the reduction and mineralization mechanisms of Cr(VI). The results indicated that the high concentration of Ca could significantly enhance the reduction and mineralization of Cr(VI). The highest reduction and mineralization efficiencies of 99.5% and 55.9% were achieved at 4 g/L Ca. Moreover, the urease activity of S. saromensis in the experimental group was up to 13.28 U/mg NH-N. Besides, the characteristic results revealed that Cr(VI) and reduced Cr(III) were absorbed on the surface or got into the interspace of CaCO, which produced a new stable phase (CaCrO(CO)). Overall, the combination of S. saromensis and MICP technology might be a high-efficiency and environmentally friendly strategy for further application in the Cr(VI)-containing groundwater.
Topics: Chromium; Carbonates; Sporosarcina; Calcium Carbonate
PubMed: 37442938
DOI: 10.1007/s11356-023-28536-3 -
Bioresource Technology Nov 2021This study aims to construct a high-temperature-resistant microbial consortium to effectively degrade oily food waste by Fed-in-situ biological reduction treatment...
This study aims to construct a high-temperature-resistant microbial consortium to effectively degrade oily food waste by Fed-in-situ biological reduction treatment (FBRT). Oil degrading bacteria were screened under thermophilic conditions of mineral salt medium with increased oil content. The oil degradation and emulsification ability of each stain was evaluated and their synergetic improvement was further confirmed. Consortium of Bacillus tequilensis, Bacillus licheniformis, Bacillus sonorensis and Ureibacillus thermosphaericus was selected and applicated as bacterial agents in FBRT under 55 °C. Changes in pH, moisture, bacterial community and key components of food waste were monitored for 5 days during processing. Facilitated by the bacterial consortium, FBRT gave superior total mass reduction (86.61 ± 0.58% vs. 67.25 ± 1.63%) and non-volatile solids reduction (65.91 ± 1.53% vs. 28.53 ± 2.29%) compared with negative control, the feasibility and efficiency of present FBRT providing a promising in-situ disposal strategy for rapid reduction of oily food waste.
Topics: Bacillus; Biodegradation, Environmental; Food; Microbial Consortia; Planococcaceae; Refuse Disposal; Temperature
PubMed: 34339998
DOI: 10.1016/j.biortech.2021.125635 -
Nature Communications Apr 2021Studies in humans and laboratory animals link stable gut microbiome "enterotypes" with long-term diet and host health. Understanding how this paradigm manifests in wild...
Studies in humans and laboratory animals link stable gut microbiome "enterotypes" with long-term diet and host health. Understanding how this paradigm manifests in wild herbivores could provide a mechanistic explanation of the relationships between microbiome dynamics, changes in dietary resources, and outcomes for host health. We identify two putative enterotypes in the African buffalo gut microbiome. The enterotype prevalent under resource-abundant dietary regimes, regardless of environmental conditions, has high richness, low between- and within-host beta diversity, and enrichment of genus Ruminococcaceae-UCG-005. The second enterotype, prevalent under restricted dietary conditions, has reduced richness, elevated beta diversity, and enrichment of genus Solibacillus. Population-level gamma diversity is maintained during resource restriction by increased beta diversity between individuals, suggesting a mechanism for population-level microbiome resilience. We identify three pathogens associated with microbiome variation depending on host diet, indicating that nutritional background may impact microbiome-pathogen dynamics. Overall, this study reveals diet-driven enterotype plasticity, illustrates ecological processes that maintain microbiome diversity, and identifies potential associations between diet, enterotype, and disease.
Topics: Animals; Buffaloes; Communicable Diseases; DNA, Bacterial; Feces; Feeding Behavior; Firmicutes; Gastrointestinal Microbiome; Incidence; Metagenomics; Phylogeny; Planococcaceae; Prevalence; RNA, Ribosomal, 16S; South Africa; Symbiosis
PubMed: 33859184
DOI: 10.1038/s41467-021-22510-8 -
Environmental Science & Technology Aug 2021Microbially induced calcite precipitation is a biomineralization process with numerous civil engineering and ground improvement applications. In replicate soil columns,...
Microbially induced calcite precipitation is a biomineralization process with numerous civil engineering and ground improvement applications. In replicate soil columns, the efficacy and microbial composition of soil bioaugmented with the ureolytic bacterium were compared to a biostimulation method that enriches native ureolytic soil bacteria under conditions analogous to field implementation. The selective enrichment resulting from sequential stimulation treatments strongly selected for Firmicutes (>97%), with and comprising 60 to 94% of high-throughput 16S rDNA sequences in each suspended community sample. Seven species of the former and two of the latter were present in greater than 10% abundance at different times, demonstrating unexpected within-genus diversity and robustness in the suspended phase of this highly selective environment. Based on longer 16S sequences, it was inferred that augmented competed poorly with natural bacteria, decreasing to below detection after nine treatments, while the native microbial community was enriched to approximately that present in the stimulated columns. These analyses were corroborated by the observed convergence in bulk ureolytic rates and calcite contents between techniques. However, a 10-fold discrepancy between the observed cell density and an activity-based estimate indicates the attached community, uncharacterized despite efforts, substantially contributes to bulk behavior.
Topics: Bacteria; Calcium Carbonate; Soil; Sporosarcina
PubMed: 34279077
DOI: 10.1021/acs.est.1c01520 -
Bioresource Technology Jul 2020Two bacterial species with the ability to produce biosurfactants were isolated from a pesticide contaminated soil and identified as Planococcus rifietoensis IITR53 and...
Two bacterial species with the ability to produce biosurfactants were isolated from a pesticide contaminated soil and identified as Planococcus rifietoensis IITR53 and Planococcus halotolerans IITR55. Formation of froth indicating the surfactant production was observed when grown in basal salt medium containing 2% glucose. The culture supernatant after 72 h showed reduction in surface tension from 72 N/m to 46 and 42 N/m for strain IITR53 and IITR55 with emulsification index of 51 and 54% respectively. The biosurfactant identified as rhamnolipid based on liquid chromatography-mass spectrometry analysis, was found to inhibit the growth of both gram- positive and negative pathogenic bacteria. Both the rhamnolipids at 40 mg/mL exhibited the release of extracellular DNA and protein content. Also at one third of the MIC, a significant generation of reactive oxygen species was recorded. These rhamnolipids effectively emulsified different vegetable oils suggesting their possible utilization as antimicrobial agent.
Topics: Bacteria; Glycolipids; Planococcus Bacteria; Pseudomonas aeruginosa; Surface-Active Agents
PubMed: 32240926
DOI: 10.1016/j.biortech.2020.123206