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Genes Jan 2021is an environmental bacterium, rarely isolated in clinical specimens, although it has been described as producing endocarditis and sepsis. Little is known about its...
is an environmental bacterium, rarely isolated in clinical specimens, although it has been described as producing endocarditis and sepsis. Little is known about its genome. Whole genome sequencing can be used to learn about the phylogeny, evolution, or pathogenicity of these isolates. Thus, the aim of this study was to analyze the resistome, virulome, and phylogenetic relationship of two strains, Ps542 and Ps799, isolated from a healthy fecal sample and a lettuce, respectively. Among all of the small number of genomes available in the National Center for Biotechnology Information (NCBI) repository, both strains were placed within one of two well-defined phylogenetic clusters. Both strains lacked antimicrobial resistance genes, but the Ps799 genome showed a MOB family relaxase. Nevertheless, this study revealed that possesses an important number of virulence factors, including a leukotoxin, flagella, pili, and the Type 2 and Type 6 Secretion Systems, that could be responsible for their pathogenesis. More phenotypical and in vivo studies are needed to deepen the association with human infections and the potential pathogenicity.
Topics: Animals; Anti-Bacterial Agents; Bacterial Proteins; Drug Resistance, Microbial; Ducks; Endocarditis; Endodeoxyribonucleases; Feces; Food Microbiology; Genome, Bacterial; Humans; Lactuca; Phylogeny; Pseudomonas mendocina; Sepsis; Virulence Factors; Whole Genome Sequencing
PubMed: 33477842
DOI: 10.3390/genes12010115 -
Foodborne Pathogens and Disease Nov 2023The purpose of this research was to analyze the functional portraits and genomic features of carbapenem-resistant carrying and . The resistance mechanism of the strain...
The purpose of this research was to analyze the functional portraits and genomic features of carbapenem-resistant carrying and . The resistance mechanism of the strain was verified by experiments. Genomic data were aligned and analyzed in the NCBI database. Growth curve measurements were used to describe the growth characteristics of the bacteria. The virulence of strain was analyzed by serum killing assay and biofilm formation assay. Plasmid conjugation experiments were performed to verify the transferability of plasmids carrying drug-resistance genes. The strain was highly resistant to carbapenems. In addition, ST typing is unknown and has been submitted to Genebank. The strain carried two carbapenemase genes, including and . Among them, was located on a 5.62832 Mb chromosome, and was located on a 172.851 Kb transferable plasmid, which was a very close relative of pIMP-NY7610 in China. The strain also had a variety of virulence genes, which were expressed in the siderophore, capsule, pilus, alginate, flagella, etc. The study suggests that the functional portrait and genomic features of carbapenem-resistant harboring and are unique to China. This outcome represents antibiotic resistance exhibited in the genus by acquiring chromosomes and plasmid genes. The monitoring and supervision of antimicrobial usage must be strengthened since the multi-drug-resistant and moderately virulent will attract much attention in the near future.
Topics: Carbapenems; Pseudomonas mendocina; beta-Lactamases; Plasmids; Drug Resistance, Microbial; Microbial Sensitivity Tests; Genomics; China; Anti-Bacterial Agents
PubMed: 37729068
DOI: 10.1089/fpd.2023.0055 -
Tropical Medicine and Infectious Disease May 2020is a Gram-negative, rod-shaped, aerobic bacterium that belongs in the family Pseudomonadaceae and has been isolated from water and soil. Even though it is thought to... (Review)
Review
is a Gram-negative, rod-shaped, aerobic bacterium that belongs in the family Pseudomonadaceae and has been isolated from water and soil. Even though it is thought to cause infections quite rarely in humans, it can cause severe infections even in immunocompetent individuals. The aim of this study was to systemically review all cases of human infection by in the literature and describe their epidemiology, microbiology, antimicrobial susceptibility, treatment and outcomes. Thus, a systematic review of PubMed for studies providing epidemiological, clinical, microbiological as well as treatment data and outcomes of infections was conducted. In total, 12 studies, containing data of 16 patients, were included. The commonest infections were infective endocarditis, central nervous system infections and skin and soft tissue infections (SSTIs). Fever was the main presenting symptom, while sepsis was evident in almost half the patients. was susceptible to most antibiotics tested. Mortality was low in all different infection types. Third or fourth generation cephalosporins and quinolones are the commonest agents used for treatment, irrespectively of the infection site.
PubMed: 32375225
DOI: 10.3390/tropicalmed5020071 -
Cureus Mar 2022is a Gram-negative bacillus from the family Pseudomonadaceae. The first -related infection was reported in 1992. Although a rare cause of infections, has been known...
is a Gram-negative bacillus from the family Pseudomonadaceae. The first -related infection was reported in 1992. Although a rare cause of infections, has been known to cause severe infections that require intensive treatment. We present the first documented case of urinary tract infection caused by An 83-year-old male with a past medical history of diabetes, hypertension, coronary artery disease, and prostate cancer with bone metastases, currently being treated with abiraterone and prednisone, presented with subjective fever, fatigue, altered mental status, dysuria, and hematuria of one-week duration. He was found to have a complicated urinary tract infection with an incidental asymptomatic COVID-19 infection on admission. The patient was empirically treated with ceftriaxone and switched to cefepime for broader coverage on day two of hospitalization. Urine culture reported the presence of with resistance only to fluoroquinolones. Ceftriaxone was reinstated. The patient was successfully treated with a seven-day course of ceftriaxone (days 1-3, days 6-7) and cefepime (days 4-5) but continued to remain inpatient for a later symptomatic COVID-19 pneumonia with discharge on day 15. The majority of . infections present as skin and soft tissue infections, infective endocarditis, meningitis, and bacteremia. Ours is the first documented case of urinary tract infection caused by particularly in an immunocompromised COVID-19 patient, and the second to report with resistance to fluoroquinolones. This report contributes to the growing literature regarding -related infections.
PubMed: 35495004
DOI: 10.7759/cureus.23583 -
Journal of Hazardous Materials Feb 2021A cocultivation of the Pseudomonas mendocina with Actinomucor elegans was developed and investigated to improve the biodegradation of polylactic acid/polybutylene...
A cocultivation of the Pseudomonas mendocina with Actinomucor elegans was developed and investigated to improve the biodegradation of polylactic acid/polybutylene adipate-co-terephthalate (PLA/PBAT). And the coculture system could produce an efficient PLA/PBAT-degrading enzymes system to degrade PLA/PBAT films. The results showed that the protease activity (11.50 U/mL) and lipase activity (40.46 U/mL) of the coculture exceeded that of the monoculture (P. mendocina of 7.31 U/mL, A. elegans of 32.47 U/mL). The degradation rate of PLA/PBAT films using the coculture system was 18.95 w% within 5 days, which was considerably higher than that of P. mendocina (12.94 w%) and A. elegans (9.27 w%) individually, suggesting that P. mendocina and A. elegans had synergistic degradation. In addition, P. mendocina and A. elegans could secrete proteases and lipases, respectively, which could catalyze the ester bonds of PLA and PBAT in PLA/PBAT films, respectively, and hydrolyze them into different monomers and oligomers as nutrition sources. Therefore, the PLA/PBAT films could be completely degraded. In this study, the PLA/PBAT films were efficiently degraded in the coculture system for the first time, which significantly improved the biodegradation of PLA/PBAT films.
Topics: Adipates; Coculture Techniques; Mucorales; Phthalic Acids; Polyenes; Polyesters; Pseudomonas mendocina
PubMed: 33264878
DOI: 10.1016/j.jhazmat.2020.123679 -
Environmental Research Aug 2023Nitrogen (N) and phosphorous (P) removal by a single bacterium could improve the biological reaction efficiency and reduce the operating cost and complexity in...
Nitrogen (N) and phosphorous (P) removal by a single bacterium could improve the biological reaction efficiency and reduce the operating cost and complexity in wastewater treatment plants (WWTPs). Here, an isolated strain was identified as Pseudomonas mendocina SCZ-2 and showed high performance of heterotrophic nitrification (HN) and aerobic denitrification (AD) without intermediate accumulation. During the AD process, the nitrate removal efficiency and rate reached a maximum of 100% and 47.70 mg/L/h, respectively, under optimal conditions of sodium citrate as carbon source, a carbon-to-nitrogen ratio of 10, a temperature of 35 °C, and shaking a speed of 200 rpm. Most importantly, the strain SCZ-2 could rapidly and simultaneously eliminate N and P with maximum NH-N, NO-N, NO-N, and PO-P removal rates of 14.38, 17.77, 20.13 mg N/L/h, and 2.93 mg P/L/h, respectively. Both the N and P degradation curves matched well with the modified Gompertz model. Moreover, the amplification results of functional genes, whole genome sequencing, and enzyme activity tests provided theoretical support for simultaneous N and P removal pathways. This study deepens our understanding of the role of HN-AD bacteria and provides more options for simultaneous N and P removal from actual sewage.
Topics: Denitrification; Pseudomonas mendocina; Nitrogen; Aerobiosis; Nitrification; Phosphorus; Carbon; Nitrites
PubMed: 37149028
DOI: 10.1016/j.envres.2023.116062 -
Bioresource Technology Jan 2021Six bacterial strains with simultaneous nitrification-denitrification abilities were isolated from a Beijing sewage treatment plant to improve nitrogen biodegradation...
Six bacterial strains with simultaneous nitrification-denitrification abilities were isolated from a Beijing sewage treatment plant to improve nitrogen biodegradation efficiency. One of these strains, X49, was identified as Pseudomonas mendocina, and was characterized as the best strain with which to rapidly degrade a high concentration of inorganic nitrogen. X49 completely converted 5-100 mg.L of ammonia in 12 h, with no nitrite accumulation; the maximum removal rate of 26.39 mg (N).L.h was achieved between 4 h and 6 h. In 16 h, the strain removed 100 mg.L nitrite and 72.61 mg.L nitrate under aerobic conditions, at degredation rates which reached 4.54 and 6.25 mg (N).L.h, respectively. Our results suggest that P. mendocina X49 achieved efficient and simultaneous nitrification and denitrification ability under heterotrophic aerobic conditions.
Topics: Aerobiosis; Ammonium Compounds; Beijing; Denitrification; Heterotrophic Processes; Nitrification; Nitrites; Nitrogen; Pseudomonas mendocina
PubMed: 33038648
DOI: 10.1016/j.biortech.2020.124198 -
MicroPublication Biology 2022experiences and microbiome have been shown to shape its responses to certain stimuli; a recent study found that grown on JUb39 exhibited increased attraction to that...
experiences and microbiome have been shown to shape its responses to certain stimuli; a recent study found that grown on JUb39 exhibited increased attraction to that same growth bacteria while also lowered repulsion to the odor 1-octanol (O’Donnell et al. 2020). This prompted us to ask whether other strains of bacteria could likewise alter responses to bacterial food and volatile chemicals. So, to expand upon current knowledge, we cultured wild-type (N2) on an unidentified ( sp?), (MSPm1), (MYb11), (JUb19), or strain ( sp?). After several generations, we examined how their choice of bacterial food was affected. In addition, we looked at their response to the olfactory stimuli 2-butanone; 2,3-butanedione; 2,3-pentanedione; and 2-nonanone, as well as their response to the gustatory stimulus sodium chloride. Interestingly, we found that growth on any of these bacterial strains led to their bacterial preferences and behavioral responses to 2-butanone; 2,3-pentanedione; diacetyl; and sodium chloride remaining unchanged. However, we also saw that showed a preference for MSPm1 and sp? to HB101, and HB101 to MYb11. Furthermore, worms that are grown on MSPm1 showed stronger attraction to a 1:10 dilution of 2-nonanone (AWB-sensed odorant) as compared to worms grown on the other bacterial strains.
PubMed: 35622520
DOI: 10.17912/micropub.biology.000535 -
International Journal of Biological... Nov 2020Pseudomonas mendocina NK-01 previously isolated by our lab is able to accumulate medium-chain-length polyhydroxyalkanoate (mcl-PHA) intracellularly and secrete alginate...
Pseudomonas mendocina NK-01 previously isolated by our lab is able to accumulate medium-chain-length polyhydroxyalkanoate (mcl-PHA) intracellularly and secrete alginate oligosaccharide (AO) to the extracellular milieu. The present study aimed at investigating whether improved production of mcl-PHA and AO by P. mendocina can be accomplished by genome reduction. In this study, 14 large genomic fragments accounting for 7.7% of the genome of P. mendocina NK-01 were sequentially deleted to generate a series of genome-reduced strains by an upp-based markerless knockout method. As a result, the intracellular ATP/ADP ratio of the strain NKU421 with the largest deletion improved by 11 times compared to NK-01. More importantly, the mcl-PHA and AO yields of NKU421 increased by 114.8% and 27.8%, respectively. Enhancing mcl-PHA and AO production by NKU421 may be attributed to improved transcriptional levels of PHA synthase genes and AO secretion-related genes. The present study suggests that rational reduction of bacterial genome is a feasible approach to construct an optimal chassis for enhanced production of bacterial metabolites. In the future, further reduction of the NKU421 genome can be expected to create high-performance chassis for the development of microbial cell factories.
Topics: Acyltransferases; Alginates; Genome, Bacterial; Metabolic Engineering; Oligosaccharides; Polyhydroxyalkanoates; Pseudomonas mendocina
PubMed: 32941898
DOI: 10.1016/j.ijbiomac.2020.09.067 -
Bioresource Technology Jan 2021The performance of nitrate removal by Pseudomonas mendocina GL6 cells immobilized on bamboo biochar was investigated. The results showed that immobilized bacterial cells...
The performance of nitrate removal by Pseudomonas mendocina GL6 cells immobilized on bamboo biochar was investigated. The results showed that immobilized bacterial cells performed better nitrate removal than the free bacterial cells, and the nitrate removal rate increased from 6.51 mg/(L·h) of free cells to 8.34 mg/(L·h) of immobilized cells. The nitrate removal of immobilized bacterial cells fitted well to the zero-order kinetics model. Moreover, bath experiments showed that immobilized bacterial cells displayed more nitrate removal capacity under different conditions than free bacterial cells due to the protection of biochar carrier. The subsequent mechanistic study suggested that biochar promoted the expression level of denitrification functional genes (napA and nirK) and electron transfer genes involved in denitrification (napB and napC), which resulted in the increase of nitrate removal efficiency. Thus, biochar-immobilized P. mendocina GL6 has much potential to remove nitrate from wastewater via aerobic denitrification.
Topics: Charcoal; Denitrification; Nitrates; Nitrogen; Pseudomonas mendocina; Sasa
PubMed: 33147528
DOI: 10.1016/j.biortech.2020.124324