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Comparative Biochemistry and... Apr 2022Polycyclic aromatic hydrocarbons (PAHs) constitute important soil contaminants derived from petroleum. Poz14 strain can degrade pyrene and naphthalene. Its genome...
Polycyclic aromatic hydrocarbons (PAHs) constitute important soil contaminants derived from petroleum. Poz14 strain can degrade pyrene and naphthalene. Its genome presented 9333 genes, among them those required for PAHs degradation. By phylogenomic analysis, the strain might be assigned to Amycolatopsis nivea. The strain was grown in glucose, pyrene, and naphthalene to compare their proteomes; 180 proteins were detected in total, and 90 of them were exclusives for xenobiotic conditions. Functions enriched with the xenobiotics belonged to transcription, translation, modification of proteins and transport of inorganic ions. Enriched pathways were pentose phosphate, proteasome and RNA degradation; in contrast, in glucose were glycolysis/gluconeogenesis and glyoxylate cycle. Proteins proposed to participate in the upper PAHs degradation were multicomponent oxygenase complexes, Rieske oxygenases, and dioxygenases; in the lower pathways were ortho-cleavage of catechol, phenylacetate, phenylpropionate, benzoate, and anthranilate. The catechol dioxygenase activity was measured and found increased when the strain was grown in naphthalene. Amycolatopsis sp. Poz14 genome and proteome revealed the PAHs degradation pathways and functions helping to contend the effects of such process.
Topics: Amycolatopsis; Biodegradation, Environmental; Metabolic Networks and Pathways; Naphthalenes; Polycyclic Aromatic Hydrocarbons; Pyrenes
PubMed: 35026398
DOI: 10.1016/j.cbpc.2022.109268 -
Biodegradation Dec 2022Bioaugmentation effectively enhances microbial bioremediation of hazardous polycyclic aromatic hydrocarbons (PAHs) from contaminated environments. While screening for...
Bioaugmentation effectively enhances microbial bioremediation of hazardous polycyclic aromatic hydrocarbons (PAHs) from contaminated environments. While screening for pyrene-degrading bacteria from a former manufactured gas plant soil (MGPS), the mixed enrichment culture was found to be more efficient in PAHs biodegradation than the culturable pure strains. Interestingly, analysis of 16S rRNA sequences revealed that the culture was dominated by a previously uncultured member of the family Rhizobiaceae. The culture utilized C1 and other methylotrophic substrates, including dimethylformamide (DMF), which was used as a solvent for supplementing the culture medium with PAHs. In the liquid medium, the culture rapidly degraded phenanthrene, pyrene, and the carcinogenic benzo(a)pyrene (BaP), when provided as the sole carbon source or with DMF as a co-substrate. The efficiency of the culture in the bioremediation of PAHs from the MGPS and a laboratory waste soil (LWS) was evaluated in bench-scale slurry systems. After 28 days, 80% of Σ16 PAHs were efficiently removed from the inoculated MGPS. Notably, the bioaugmentation achieved 90% removal of four-ringed and 60% of highly recalcitrant five- and six-ringed PAHs from the MGPS. Likewise, almost all phenanthrene, pyrene, and 65% BaP were removed from the bioaugmented LWS. This study highlights the application of the methylotrophic enrichment culture dominated by an uncultured bacterium for the efficient bioremediation of PAHs.
Topics: Biodegradation, Environmental; Polycyclic Aromatic Hydrocarbons; RNA, Ribosomal, 16S; Soil Pollutants; Soil Microbiology; Benzo(a)pyrene; Dimethylformamide; Soil; Pyrenes; Phenanthrenes; Bacteria; Carbon; Solvents
PubMed: 35976498
DOI: 10.1007/s10532-022-09996-9 -
The Science of the Total Environment Jan 2023A novel magnetic core-shell FeO@SiO@CdS embedded graphene oxide (GO) composite was prepared for the visible-light-driven photodegradation of high ring number polycyclic...
A novel magnetic core-shell FeO@SiO@CdS embedded graphene oxide (GO) composite was prepared for the visible-light-driven photodegradation of high ring number polycyclic aromatic hydrocarbons (PAHs). The potential application of GO-FeO@SiO@CdS was evaluated through the photodegradation of phenanthrene and pyrene in deionized water, tap water, and lake water, respectively. It was found that GO-FeO@SiO@CdS could remove 86.4 % of phenanthrene and 93.4 % of pyrene, suggesting its potential for the degradation of high-ring number PAHs. The density functional theory (DFT) calculations demonstrate that pyrene has more active sites attacked by free radicals. The photoelectrochemical measurement and quenching experiments indicate that GO can transfer photoelectrons efficiently, resulting in the crucial radicals (O, OH and O). More importantly, the photocatalytic activity kept almost constant during five cycles, confirming the significant anti-photocorrosion of GO-FeO@SiO@CdS. This work provides some new insights into the removal of PAHs with high-ring numbers in the natural water environment.
Topics: Photolysis; Silicon Dioxide; Polycyclic Aromatic Hydrocarbons; Phenanthrenes; Pyrenes; Water; Light
PubMed: 36209874
DOI: 10.1016/j.scitotenv.2022.159254 -
Organic & Biomolecular Chemistry Mar 2022Novel pyrene-based double aza- and diaza[4]helicenes have been prepared through a five-step synthetic sequence in overall good yields. Commercially available...
Novel pyrene-based double aza- and diaza[4]helicenes have been prepared through a five-step synthetic sequence in overall good yields. Commercially available 2,3-dihaloazines (2,3-dibromopyridine, 2,3-dichloropyrazine and 2,3-dichloroquinoxaline) were used as starting materials. The synthesis employs electrophile-induced cyclizations of -alkynyl bihetaryls as the key steps, leading to the formation of a helical skeleton. To discern the effect of merging azine and pyrene moieties within a helical skeleton, the X-ray structures, UV-vis absorption and fluorescence spectra of the helicenes were investigated and compared with those of the parent [4]helicene, aza- and diaza[4]helicenes. It was found that the emission properties of the synthesized helicenes can be modulated as a function of pH. The basicity of pyrene-based double aza[4]helicenes was estimated by the direct fluorimetric titration method; the p value was found to be equal to 1.4.
Topics: Hydrogen-Ion Concentration; Models, Molecular; Polycyclic Compounds; Pyrenes
PubMed: 35293927
DOI: 10.1039/d2ob00204c -
Chembiochem : a European Journal of... Mar 2023Heparin is a commonly used anticoagulant in clinical practice; however, excessive heparin can cause serious adverse reactions. Convenient and accurate detection of...
Heparin is a commonly used anticoagulant in clinical practice; however, excessive heparin can cause serious adverse reactions. Convenient and accurate detection of heparin levels is thus very important. In this research, a pyrene-based self-assembling fluorescent peptide PyFFRRR was designed for simple, selective, and efficient heparin detection. The guanidine groups in the arginine residues of PyFFRRR bind tightly with heparin, which is highly sulfated, through electrostatic interactions. Charge neutralization facilitated the self-assembly of PyFFRRR, resulting in its spectral response changing from deep blue monomer fluorescence to green excimer fluorescence. PyFFRRR exhibited excellent sensitivity and selectivity for ratiometric detection of heparin. The binding mechanism was investigated by using spectral and simulation tools, and structural observation. Finally, PyFFRRR was employed in human serum samples for ratiometric detection of heparin.
Topics: Humans; Heparin; Fluorescent Dyes; Peptides; Anticoagulants; Spectrometry, Fluorescence; Pyrenes
PubMed: 36592168
DOI: 10.1002/cbic.202200652 -
Biosensors Apr 2023The effective and accurate detection of the anticancer drug coralyne (COR) is highly significant for drug quality control, medication safety and good health. Although...
Integration of G-Quadruplex and Pyrene as a Simple and Efficient Ratiometric Fluorescent Platform That Programmed by Contrary Logic Pair for Highly Sensitive and Selective Coralyne (COR) Detection.
The effective and accurate detection of the anticancer drug coralyne (COR) is highly significant for drug quality control, medication safety and good health. Although various COR sensors have been reported in recent years, previous ones can only exhibit single-signal output (turn ON or turn OFF) with poor reliability and anti-interference ability. Therefore, exploring novel platform with dual-signal response for COR detection is urgently needed. Herein, we reported the first ratiometric fluorescent platform for highly sensitive and selective COR detection by integrating G-quadruplex (G4) and Pyrene (Py) as signal probes and harnessing A-COR-A interaction. In the absence of COR, the platform shows a low fluorescence signal of PPIX (F) and a high one of Py monomer (F). With the addition of COR, two delicately designed poly-A ssDNAs will hybridize with each other via A-COR-A coordination to form complete G4, yielding the increased fluorescence signal of PPIX and the decreased one of Py due to the formation of Py excimer. Based on the above mechanism, we constructed a simple and efficient sensor that could realize the ratiometric fluorescent detection of COR with high sensitivity and selectivity. A linear relationship between F/F and COR's concentration is obtained in the range from 1 nM to 8 μM. And the limit of detection of COR could reach to as low as 0.63 nM without any amplification, which is much lower than that of most COR sensors reported so far. Notably, the logical analysis of COR can be carried out under the control of a "YES-NOT" contrary logic pair, enabling the smart dual-channel response with an adequate S/N ratio and improved reliability and anti-interference ability. Moreover, this system also presents satisfactory performance in fetal bovine serum (FBS) samples.
Topics: Fluorescent Dyes; Reproducibility of Results; Pyrenes; Logic; Spectrometry, Fluorescence
PubMed: 37185564
DOI: 10.3390/bios13040489 -
Ecotoxicology (London, England) Sep 2021Two pyrene-degrading strains, Pseudomonas aeruginosa PA06 and Achromobacter sp. AC15 were co-incubated in equal proportions as a microbiological consortium and could...
Two pyrene-degrading strains, Pseudomonas aeruginosa PA06 and Achromobacter sp. AC15 were co-incubated in equal proportions as a microbiological consortium and could enhance the degradation of pyrene. The enzymatic activities of the catechol 1,2-dioxygenase (C12O) and 2,3-dioxygenase activities (C23O) were produced complementary expression by P. aeruginosa PA06 and Achromobacter sp. AC15, respectively. Meanwhile, results showed that pyrene degradation was sufficiently promoted in the presence of sodium citrate as a co-metabolic carbon source, likely a result of enhanced biomass and biosurfactant production. The optimized dosage and ideal initial pHs were 1.4 g L and 5.5, respectively. We also analyzed the rate constant of pyrene degradation, cell growth, and enzyme activity. Results show that P. aeruginosa PA06 had a better effect than Achromobacter sp. AC15 in bacterial growth. However, the C23O or C12O activity produced by Achromobacter sp. AC15 continued at a similar or even faster than that of P. aeruginosa PA06. The mixed bacteria had a better effect than any single bacteria, suggesting the strains worked synergistically to enhance the degradation efficiency. In the co-metabolism system of 600 mg/L pyrene and 1.4 g/L sodium citrate, pyrene degradation reached 74.6%, was 1.57 times, 2.06 times, and 3.89 times that of the mix-culture strains, single PA06 and single AC15 without sodium citrate, respectively. Overall, these findings are valuable as a potential tool for the bioremediation of high-molecular-weight PAHs.
Topics: Achromobacter; Biodegradation, Environmental; Carbon; Pseudomonas aeruginosa; Pyrenes; Sodium Citrate
PubMed: 32844301
DOI: 10.1007/s10646-020-02268-3 -
Chemosphere Feb 2020Combined pollution caused by polybrominated diphenyl ethers (PBDEs) and polycyclic aromatic hydrocarbons (PAHs) in mangrove wetlands is serious, with their remediation...
Combined pollution caused by polybrominated diphenyl ethers (PBDEs) and polycyclic aromatic hydrocarbons (PAHs) in mangrove wetlands is serious, with their remediation to be been paid more and more attention. However, little is known about the combined impact of PAHs and mangrove species on removal of PBDEs in contaminated soils. In this study, BDE-209 and pyrene were selected and a 9 months experiment was conducted to explore how BDE-209 removal in contaminated soil varied with pyrene addition and Kandelia obovata planting, and to clarify corresponding microbial responses. Results showed that BDE-209 removals in soil induced by pyrene addition or K. obovata planting were significant and stable after 6 months, with the lowest levels of BDE-209 in combined pyrene addition with K. obovata planting. Unexpected, root uptake of BDE-209 in K. obovata was limited for BDE-209 removal in soil, which was verified by lower total amount of BDE-209 bioaccumulated in K. obovata's root. In soil without K. obovata planting, BDE-209 removal caused by pyrene addition coexisted with changed bacterial abundance at phylum Planctomycetes and Chloroflexi, class Planctomycetacia, and genus Blastopirellula. K. obovata-induced removal of BDE-209 in soil may be related to bacterial enrichment in phylum Proteobacteria, class Gammaproteobacteria and genus Ilumatobacter, Gaiella. Thus, in BDE-209 contaminated soil, microbial community responses induced by pyrene addition and K. obovata planting were different at phylum, class and genus levels. This is the first study demonstrating that pyrene addition and K. obovata planting could improve BDE-209 removal, and differently affected the corresponding responses of microbial communities.
Topics: Pyrenes; Rhizophoraceae; Soil; Soil Pollutants
PubMed: 31574439
DOI: 10.1016/j.chemosphere.2019.124873 -
International Journal of Biological... Feb 2021Polycyclic aromatic hydrocarbons, distributing extensively in the soil, would potentially threaten the soil organisms (Eisenia fetida) by triggering oxidative stress. As...
Polycyclic aromatic hydrocarbons, distributing extensively in the soil, would potentially threaten the soil organisms (Eisenia fetida) by triggering oxidative stress. As a ubiquitous antioxidant enzyme, catalase can protect organisms from oxidative damage. To reveal the potential impact of polycyclic aromatic hydrocarbon pyrene (Pyr) on catalase (CAT) and the possible protective effect of Ascorbic acid (vitamin C), multi-spectral and molecular docking techniques were used to investigate the influence of structure and function of catalase by pyrene. Fluorescence and circular dichroism analysis showed that pyrene would induce the microenvironmental changes of CAT amino acid residues and increase the α-helix in the secondary structure. Molecular simulation results indicated that the main binding force of pyrene around the active center of CAT is hydrogen bonding force. Furthermore, pyrene inhibited catalase activity to 69.9% compared with the blank group, but the degree of inhibition was significantly weakened after vitamin C added into the research group. Cell level experiments showed that pyrene can increase the level of ROS in the body cavity cell of earthworms, and put the cells under the threat of potential oxidative damage. Antioxidants-vitamin C has a protective effect on catalase and maintains the stability of intracellular ROS levels to a certain extent.
Topics: Amino Acids; Animals; Antioxidants; Ascorbic Acid; Catalase; Circular Dichroism; Hydrogen Bonding; Models, Molecular; Molecular Docking Simulation; Oligochaeta; Oxidative Stress; Protein Conformation; Protein Structure, Secondary; Pyrenes; Reactive Oxygen Species; Soil Pollutants; Spectrometry, Fluorescence; Static Electricity
PubMed: 33418042
DOI: 10.1016/j.ijbiomac.2020.12.169 -
Environmental Science and Pollution... Aug 2023Due to the high exposure toxicity and individual variability of polycyclic aromatic hydrocarbons (PAHs), it is difficult to accurately characterize the actual exposure...
Due to the high exposure toxicity and individual variability of polycyclic aromatic hydrocarbons (PAHs), it is difficult to accurately characterize the actual exposure of exposed individuals through external exposure detection. In this study, the monohydroxyl metabolites of naphthalene, phenanthrene, pyrene, and 9-fluorenone were identified in the urine of low-dose PAH-exposed individuals based on ultra-performance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS), and their concentrations were monitored for 15 consecutive days after exposure. The results showed that the metabolite concentrations of naphthalene, phenanthrene, and pyrene were basically the same, and all of them reached the maximum value at day 8. In contrast, the metabolite of 9-fluorenone reached its maximum value on day 2. This study showed that the four metabolites were strongly correlated with their parent PAH exposure, with a wide detection window, and their assays were specific, sensitive, and reliable, while the sampling difficulty was low, so the four hydroxylated PAHs may be potential low-dose biomarkers of PAH internal exposure. This study will provide methodological and data support for further health risk studies involving internal exposure to organic pollutants such as PAHs.
Topics: Humans; Polycyclic Aromatic Hydrocarbons; Pyrenes; Phenanthrenes; Naphthalenes; Biomarkers; Environmental Monitoring
PubMed: 37470974
DOI: 10.1007/s11356-023-28600-y