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Frontiers in Bioengineering and... 2022For total knee arthroplasty (TKA), the optimal rotational position of the femoral component is felt to be critically important. The current knee joint kinematics...
For total knee arthroplasty (TKA), the optimal rotational position of the femoral component is felt to be critically important. The current knee joint kinematics measurement technology is unable to identify the exact rotation axis of the knee joint, the main reasons being low measurement accuracy and insufficient three-dimensional data (2D-3D image matching technology). In order to improve the effect of TKA surgery, we proposed a knee joint kinematics measurement method, based on the MRI technology, and verified its measurement accuracy. We then employed this method to identify the personalized optimal rotation axis of the knee joint for TKA patients. The purpose of the study was 1) to propose a method for measuring knee joint kinematics and verify its accuracy and 2) to propose a method for determining the optimal rotation axis of knee joint for TKA surgery, based on accurate kinematic measurement results. The experiment was divided into two parts: and . The purpose of the experiment was to verify the measurement accuracy of our method. We fixed two aquarium stones (approximately 10 cm * 10 cm * 10 cm in size, close to the size of the distal femur and proximal tibia) firmly on the fixed and moving arms of the goniometer/vernier caliper with glue and immersed the aquarium stones in the water to capture MRI images. The MRI images were then processed with MATLAB software, and the relative motion of the two aquarium stones was measured. The measurement accuracy of our method was verified the scale reading of the moving arm on the goniometer/vernier caliper. , 36 healthy elderly participants (22 females, 14 males) were recruited from the local community; our method was then employed to measure the relative motion of the tibia and femur and to observe the rollback and screw home motion of the medial/lateral condyle of the femur, which was identified as specific kinematic features of the knee joint. , all measurements were accurate to <1 mm and <1°. , all knee measurements showed rollback motion (the rollback distance of the medial femoral condyle was 18.1 ± 3.7 mm and that of the lateral condyle was 31.1 ± 7.3 mm) and screw home motion. In the application scenario of knee joint kinematics measurement, our method has an accuracy of <1° of rotation angle and <1 mm of translation for all reference points, and it can be employed to identify the most stable axis of the knee joint. Using our method to accumulate data on the knee rotation axis of more subjects to establish an average rotation axis of a given population may help in knee prosthesis design and reduce the patient dissatisfaction rate. Individually measuring the patient's rotation axis before TKA surgery and adjusting the prosthesis installation in TKA may further reduce the patient dissatisfaction rate, and automatic computer measurement may be realized in the future, but it is still time-consuming for now.
PubMed: 36601392
DOI: 10.3389/fbioe.2022.904012 -
Cureus May 2023The aim of this study was to investigate the morphological features of the distal femur, with a specific focus on the facies patellaris femoris.
INTRODUCTION
The aim of this study was to investigate the morphological features of the distal femur, with a specific focus on the facies patellaris femoris.
METHODS
A total of 45 dry femurs from adult individuals (24 right, 21 left) were used for the study. Measurements were taken using a calibrated digital vernier caliper and a contour gauge.
RESULTS
Anteroposterior (AP) measurements were taken for the medial and lateral condyles of the femur, as well as the articular surfaces of the facies patellaris, sulcus height (51.186±3.81mm), trochlear depth (7.436±1.19mm), and trochlear index (2.295±0.06mm). The results showed that the width of the facies patellaris had a significant positive correlation with the trochlear depth and trochlear index. The length of the facies patellaris was positively correlated with the AP length of the medial condyle and sulcus height, although it was not statistically significant. Additionally, there was a statistically significant positive correlation between the length, width, and medial and lateral articular surfaces of the facies patellaris (p<0.005).
CONCLUSION
Understanding the relationship between the morphometry of the medial and lateral condyles of the distal femur and the morphometry of the facies patellaris, sulcus height, trochlear depth, and trochlear index and examining the anatomy of the distal femur and patella in individuals are crucial factors for determining appropriate medical treatment and implant selection and compatibility. The findings of this study are expected to contribute to clinicians' interventions in this region (total knee arthroplasty/replacement operation etc.). These data can also be used by implant designers and forensic experts during investigations.
PubMed: 37303382
DOI: 10.7759/cureus.38839 -
Cureus Mar 2022Background Kidney size determination and sonographic follow-up are important in clinical diagnosis and treatment in children. Various anthropometric measurements are...
Background Kidney size determination and sonographic follow-up are important in clinical diagnosis and treatment in children. Various anthropometric measurements are correlated with gestational age and birth weight and are used to identify high-risk babies in need of early interventions. Although foot length has emerged as a simple and reliable anthropometric measurement, it is not correlated with kidney size, except in the fetal period. This study was undertaken to find a correlation between foot length and kidney dimensions and estimate kidney size by finding regression equations in newborns. Methods We conducted a cross-sectional study and 216 newborns were enrolled at a tertiary care hospital. Foot length was measured by digital Vernier calipers and kidney dimensions were measured by ultrasonography. The Pearson correlation coefficient and simple linear regression tests were used to determine the relationship between foot length and kidney dimensions. Results Foot lengths and kidney dimensions were comparable in males and females as well as on the right and left sides, except for kidney length, which was found to be longer in males. Both right and left foot lengths showed highly significant (p<0.001) but small, positive correlations with corresponding side kidney length, breadth, and area, with R-values ranging from 0.2874 to 0.3668. However, the correlation between birth weight and foot length was significant, positive, and moderate (r=0.6962 and 0.6923 for right and left foot lengths, respectively). The regression equation for estimation of kidney size from foot length was obtained but the variance explained was small (e.g. R=0.1325 for right kidney length). Out of 216 babies in our study, 10 babies had a renal anomaly. Conclusions We found a significant but small, positive correlation between foot length and kidney dimensions. Only 13.25% of the variance in kidney length was associated with foot length. Birth weight also had a significant and positive but small correlation with kidney dimensions. However, the correlation of birth weight with foot length was moderate, and a 57.14% variance in foot length was associated with birth weight. Multivariate regression analysis with more anthropometric parameters and gestational age may help in finding a better estimation of kidney size.
PubMed: 35475101
DOI: 10.7759/cureus.23352 -
Bioengineered May 2022The study is aimed to determine the effects of cynarin (Cyn) on mice with gouty arthritis (GA) induced by monosodium urate (MSU). We measured swelling in the hind paws...
The study is aimed to determine the effects of cynarin (Cyn) on mice with gouty arthritis (GA) induced by monosodium urate (MSU). We measured swelling in the hind paws of mice using Vernier calipers and ultrasound. The liver, kidney, and hind paws were stained with hematoxylin-eosin, and M1 type macrophages were detected in the hind paws using anti-F4/80 and anti-iNOS antibodies. The mRNA expression of inflammatory factors in bone marrow-derived macrophages (BMDMs) and in the hind paws was detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family pyrin domain containing 3 (NLRP3) inflammasomes and the nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways were analyzed via western blotting. Cyn was detected using Cell Counting Kit-8 (CCK-8). Cyn treatment reduced hind paw swelling and M1 macrophage infiltration, suppressed the mRNA expression of inflammatory factors, and inhibited NLRP3 inflammasome activation , in addition to inhibiting the phosphorylation of IKKa/β, p65, and c-Jun NH 2-terminal kinase (JNK). Furthermore, Cyn exerted anti-inflammatory and anti-swelling effects in mice with GA by regulating the NF-κB and JNK pathways and NLRP3 inflammasomes.
Topics: Animals; Arthritis, Gouty; Cinnamates; Inflammasomes; Mice; NF-kappa B; NLR Family, Pyrin Domain-Containing 3 Protein; RNA, Messenger; Uric Acid
PubMed: 35546047
DOI: 10.1080/21655979.2022.2072055 -
Acta Medica Academica Apr 2022The objective of this study was to study the morphometry of the styloid process of the temporal bone and the prevalence of an elongated styloid process in relation to...
OBJECTIVE
The objective of this study was to study the morphometry of the styloid process of the temporal bone and the prevalence of an elongated styloid process in relation to side and gender.
MATERIAL AND METHOD
The present study included 200 human skulls which were procured from the rich osteological collections of the Department of Anatomy, Faculty of Medicine, University of Sarajevo. The styloid process was observed macroscopically on both sides of all the skulls and elongations, if any, were noted. The lengths of the styloid processes were measured using digital vernier calipers. The measurements were taken from the point of emergence of the process (base) up to the tip.
RESULTS
Out of 200 specimens, only 14 cases (7%) exhibited an elongated styloid process. The mean length of the styloid process was 25.8±4.68 mm and 24.2±4.54 mm for the right and left sides, respectively. The size of the styloid process did not different significantly between the two sides (P=0.724). The mean length of the styloid process was 24.05±3.54 mm in females and 25.95±5.68 mm in males, and the difference was statistically significant (P=0.023).
CONCLUSION
The study and knowledge of the anatomical variations of the styloid process in the Bosnian population may help clinicians to diagnose Eagle's syndrome. Knowledge of this disorder can prevent the worsening of the painful symptoms related to an elongated styloid process.
Topics: Female; Humans; Male; Ossification, Heterotopic; Pain; Temporal Bone
PubMed: 35695402
DOI: 10.5644/ama2006-124.369 -
Oman Journal of Ophthalmology 2023The aim of the study was to analyze orbitofacial anthropometric parameters such as inner and outer canthal distances (ICD and OCD), palpebral fissure length (PFL),...
AIM
The aim of the study was to analyze orbitofacial anthropometric parameters such as inner and outer canthal distances (ICD and OCD), palpebral fissure length (PFL), interpupillary distance (IPD), and canthal index (CI) in children with pseudostrabismus and to compare the measured IPD (mIPD) with calculated IPD (cIPD).
MATERIALS AND METHODS
This was a prospective study of sixty children (6 months-18 years) with pseudostrabismus. ICD, OCD, PFL, and IPD were measured by digital Vernier caliper. The formula used was cIPD: 0.21+0.24 ICD+0.58°CD for males and 1.4+0.31 ICD+0.41°CD for females. Values measured by caliper were compared with that calculated by the formula. The formula used was CI: ICD × 100/OCD. Data were analyzed statistically.
RESULTS
The mean age was 6.66 ± 3.57 years. Telecanthus was the most common finding (55%). The mean ICD and OCD in males were 30.89 ± 3.33 mm and 87.96 ± 8.09 mm and in females were 30.91 ± 3.05 and 86.22 ± 6.81 mm, respectively. The mean right eye PFL in males was 28.53 ± 2.63 mm and in females was 27.66 ± 2.22 mm and left eye PFL in males was 28.53 mm ± 2.63 and in females was 27.66 ± 2.22 mm. CI in males was 35.10 ± 1.65 and in females was 35.84 ± 1.71. Mean mIPD and cIPD: male - 55.37 ± 4.75 mm and 58.56 ± 5.34 mm, female - 53.32 ± 4.74 mm and 46.26 ± 3.71 mm. A good agreement was found between mIPD and cIPD.
CONCLUSION
This study helps in documenting the anthropometric pattern of the orbitofacial parameters in children with pseudostrabismus which can act as reference data. This helps in the management of orbitofacial, craniofacial syndromes/deformities and lid reconstructive surgeries in retaining ethnical features and obtaining better function. In children's spectacle frame 1 and lens making, where measuring IPD is difficult, cIPD can be a simple alternative.
PubMed: 38059076
DOI: 10.4103/ojo.ojo_326_22 -
Frontiers in Surgery 2021It is important to select appropriate screws in orthopedic surgeries, as excessively long or too short a screw may results failure of the surgeries. This study explored...
It is important to select appropriate screws in orthopedic surgeries, as excessively long or too short a screw may results failure of the surgeries. This study explored factors that affect the accuracy of measurements in terms of the experience of the surgeons, passage of drilled holes and different depth gauges. Holes were drilled into fresh porcine femurs with skin in three passages, straight drilling through the metaphysis, straight drilling through the diaphysis, and angled drilling through the diaphysis. Surgeons with different surgical experiences measured the holes with the same depth gauge and using a vernier caliper as gold standard. The length of selected screws, and the time each surgeon spent were recorded. The measurement accuracy was compared based on the experiences of the surgeons and the passage of drilled holes. Further, parameters of depth gauges and 12-mm cortical bone screws from five different manufacturers were measured. A total of 13 surgeons participated in 585 measurements in this study, and each surgeon completed 45 measurements. For the surgeons in the senior, intermediate, and junior groups, the average time spent in measurements was 689, 833, and 785 s with an accuracy of 57.0, 42.2, and 31.5%, respectively. The accuracy and measurement efficiency were significantly different among the groups of surgeons ( < 0.001). The accuracy of measurements was 45.1% for straight metaphyseal drilling, 43.6% for straight diaphyseal drilling, and 33.3% for angled diaphyseal drilling ( = 0.036). Parameters of depth gauges and screws varied among different manufacturers. Both observer factor and objective factors could affect the accuracy of depth gauge measurement. Increased surgeon's experience was associated with improvements in the accuracy rate and measurement efficiency of drilled holes based on the depth gauge. The accuracy rate varied with hole passages, being the lowest for angled drilled holes.
PubMed: 35096957
DOI: 10.3389/fsurg.2021.774682 -
Frontiers in Neuroscience 2022Obtaining accurate axial length (AL) is very important for the establishment of animal models of myopia. The purpose of this study is to compare the accuracy of Quantel...
BACKGROUND
Obtaining accurate axial length (AL) is very important for the establishment of animal models of myopia. The purpose of this study is to compare the accuracy of Quantel A-B scan, OD-1 A scan, and vernier caliper in measuring AL in Sprague Dawley (SD) rats.
METHODS
In total, 60 5-week-old SD rats were divided into female rat group ( = 30) and male rat group ( = 30). Quantel A-B scan and OD-1 A scan were, respectively, used to measure the AL of both eyes of each living rat, and vernier caliper was used to measure the anterior-posterior diameter of each rat's eyeball. Besides, the correlation between refractive error (RE) and AL measured by different instruments was evaluated, and the accuracy of the three measurement methods was compared according to gender and left/right eyes.
RESULTS
There were significant differences in AL and diopter of SD rats at the same age ( < 0.05). the AL of male rats was greater than that of female rats, while diopter (D) was the opposite; There was no significant difference in AL and D between left and right eyes in the same SD rats ( > 0.05); There were statistical differences among the three measurement methods ( < 0.05), AL measured by vernier caliper was the largest, followed by Quantel A-B scan, OD-1 A scan; Difference in AL between male and female was not statistically significant between the results obtained by Quantel A-B scan and vernier caliper ( > 0.05), but there were statistically significant differences between the other two measurement methods ( < 0.05).
CONCLUSION
Sex is the influencing factor of AL and RE. Imaging measurement can accurately measure the AL in living small rodents. Compared with OD-1 A scan, Quantel A-B scan may be more accurate.
PubMed: 36685229
DOI: 10.3389/fnins.2022.1106904 -
Journal of Cancer Research and Clinical... Dec 2023Previously, albendazole (ABZ) has been reported as an anti-parasitic drug rather than anti-tumor drug. Our study aim to investigate whether ABZ also has a potential...
BACKGROUND
Previously, albendazole (ABZ) has been reported as an anti-parasitic drug rather than anti-tumor drug. Our study aim to investigate whether ABZ also has a potential anti-tumor effect by shaping the tumor immune microenvironment and interrogate whether ABZ could synergize with the PD-L1 blockade.
METHODS
C57BL/6 mice (C57) were intravenously injected with B16F10-luciferase (B16-luc) cells to establish a lung metastatic melanoma model and subcutaneously inoculated with B16-luc cells to establish a subcutaneous tumor model. The tumor volume and tumor metastasis loci of the mice were measured by a vernier caliper and in vivo imaging. RNA sequencing was performed to analyze the different genes and pathways of immune cells in the tumors. Flow cytometry and immunofluorescence were used to analyze the different subsets of tumor-infiltrating immune cells.
RESULTS
The results suggested that ABZ significantly inhibited lung melanoma metastasis with decreased fluorescence intensity and nodule score and mediated the regression of subcutaneous melanoma in mice with decreased tumor volume. Moreover, RNA sequencing results showed that ABZ regulated the gene expression levels and pathways of immune cells in the tumor microenvironment (TME). Meanwhile, flow cytometry and immunofluorescence showed that the number and percentage of CD8 T cells, CD4 T cells, and T1 cells were enhanced in tumors after ABZ treatment. Furthermore, the combination of ABZ and anti-PD-L1 treatment significantly potentiated anti-tumor efficacy in both lung metastasis and subcutaneous melanoma models and mediated an increase in the percentage of CD8 T cells, CD4 T cells, and T1 cells as compared to the control group.
CONCLUSION
ABZ inhibits melanoma growth and metastasis. Moreover, ABZ synergized with PD-L1 blockade mediates tumor regression.
Topics: Mice; Animals; CD8-Positive T-Lymphocytes; Albendazole; B7-H1 Antigen; Mice, Inbred C57BL; Immunotherapy; Melanoma; Lung Neoplasms; Tumor Microenvironment; Cell Line, Tumor
PubMed: 37730912
DOI: 10.1007/s00432-023-05415-5 -
Nigerian Journal of Clinical Practice Nov 2023Corneal diameter (CD) measurement in newborns is essential for the detection of blinding ocular pathologies. It requires specially trained personnel, contact techniques,...
BACKGROUND
Corneal diameter (CD) measurement in newborns is essential for the detection of blinding ocular pathologies. It requires specially trained personnel, contact techniques, and the use of anesthesia. Smartphone use may obviate these challenges.
AIM
This study compares CD measurement among newborns using a Smartphone and manual calipers.
SUBJECTS AND METHODS
This is a cross-sectional comparative study done at Alex Ekwueme Federal University Teaching Hospital, Abakaliki, Nigeria. Consecutively selected stable term neonates whose mothers consented to the study and met the inclusion criteria had their CDs measured with both calipers and smartphones and the values compared. Mean, range, and standard deviation were calculated using Statistical Package for the Social Sciences (SPSS) IBM (version 22), while paired t-test calculated the difference in mean CD measurements between both methods. Pearson's correlation and Bland-Altman's analysis determined the correlation and agreement between both methods, respectively. Inter and intraobserver repeatability were assessed. A P value less than 0.05 was taken as statistically significant.
RESULTS
The mean CD measurement using calliper method was 10.01 ± 0.29mm and 10.03 ± 0.24 mm right eye (RE) and left eyes (LE), respectively) for investigator 1 and 10.16 ± 0.25 mm and 10.19 ± 0.21 mm (RE, LE) for investigator 2. Using smartphone measurement, mean CD measurements of 9.98 ± 0.21 mm and 10.00 ± 0.29 mm (RE, LE) were observed for investigator 1 and 10.07 ± 0.19 mm and 10.06 ± 0.21 mm (RE, LE) for investigator 2. The difference in mean CD for both instruments and by both investigators was not statistically significant (P value > 0.05).
CONCLUSION
Corneal diameter measurement in neonates using smartphone technology does not deviate significantly from caliper measurement; it is safe, easy to use, and advocated.
Topics: Female; Humans; Infant, Newborn; Smartphone; Cross-Sectional Studies; Eye; Mothers; Nigeria; Reproducibility of Results
PubMed: 38044784
DOI: 10.4103/njcp.njcp_490_23