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Persoonia Jun 2020species are common soil fungi but also known as agents of human infections (mucormycosis) and used in food production and biotechnology. is the species that is most...
species are common soil fungi but also known as agents of human infections (mucormycosis) and used in food production and biotechnology. is the species that is most frequently isolated from clinical sources. The taxonomy of and its close relatives ( complex - MCC) is still based on morphology and mating behaviour. The aim of the present study was a revised taxonomy of the MCC using a polyphasic approach. Using a set of 100 strains molecular phylogenetic analysis of five markers (ITS, , , , and , introduced here) were performed, combined with phenotypic studies, mating tests and the determination of the maximum growth temperatures. The multi-locus analyses revealed 16 phylogenetic species of which 14 showed distinct phenotypical traits and were recognised as discrete species. Five of these species are introduced as novel taxa: sp. nov., sp. nov., sp. nov., sp. nov., and sp. nov. The former formae of represent one or two separate species. In the MCC, the simple presence of well-shaped zygospores only indicates a close relation of both strains, but not necessarily conspecificity. Seven species of the MCC have been implemented in human infection: , , , and .
PubMed: 33116336
DOI: 10.3767/persoonia.2020.44.03 -
Antimicrobial Agents and Chemotherapy Aug 2019Recently, the species concept of opportunistic and its relatives has been revised, resulting in the recognition of its classical formae as independent species and the...
Recently, the species concept of opportunistic and its relatives has been revised, resulting in the recognition of its classical formae as independent species and the description of new species. In this study, we used isolates of all clinically relevant species and performed susceptibility testing using the EUCAST reference method to identify potential species-specific susceptibility patterns. susceptibility profiles of 101 mucoralean strains belonging to the genus (72), the closely related species (3), (12), (10), and (4) to six antifungals (amphotericin B, natamycin, terbinaļ¬ne, isavuconazole, itraconazole, and posaconazole) were determined. The most active drug for all Mucorales was amphotericin B. Antifungal susceptibility profiles of pathogenic species were specific for isavuconazole, itraconazole, and posaconazole. The species formerly united in showed clear differences in their antifungal susceptibilities. , , ( f. ), and exhibited high MICs to all azoles tested. presented high MICs for isavuconazole and posaconazole, and and showed high MICs for isavuconazole. MIC values of spp. for posaconazole, isavuconazole, and itraconazole were high compared to those for and the Lichtheimiaceae ( and ). Molecular identification combined with susceptibility testing is recommended for species, especially if azoles are applied in treatment.
Topics: Amphotericin B; Antifungal Agents; Humans; Itraconazole; Microbial Sensitivity Tests; Mucor; Mucormycosis; Natamycin; Nitriles; Pyridines; Rhizopus; Species Specificity; Terbinafine; Triazoles
PubMed: 31182532
DOI: 10.1128/AAC.00653-19