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International Journal of Reproductive... Mar 2021The role of genital species, genital species, and the most prevalent sexually transmitted bacteria, in male infertility are still not clear. Different reports about...
BACKGROUND
The role of genital species, genital species, and the most prevalent sexually transmitted bacteria, in male infertility are still not clear. Different reports about the impact of these bacteria on semen quality are controversial.
OBJECTIVE
This study was proposed to determine the frequency of bacteriospermia in men and investigate the relationship between the presence of these bacteria and semen quality using molecular assay.
MATERIALS AND METHODS
In this cross-sectional study, 200 semen samples obtained from men attending the research and clinical centers for fertility in Kerman, Iran, between July and December 2019 were analyzed for semen volume, progressive motility, non-progressive motility, total progressive motility, and viability according to the World Health Organization guidelines. The polymerase chain reaction was used for the detection of related bacteria.
RESULTS
The mean values of volume, progressive motility, non-progressive motility, total progressive motility, and viability were significantly lower in infertile men (p 0.001). Statistically significant correlations were observed between the presence of and progressive sperm motility, and semen volume, and the sperm normal form, and and the sperm progressive motility and viability. Logistic regression analysis showed that (OR = 8.06, p 0.001) and (OR = 16, p = 0.01) were significantly associated with male infertility.
CONCLUSION
During the infertility assessment, clinicians should consider of role and in male infertility. Screening test particularly for asymptomatic individuals is recommended.
PubMed: 35571500
DOI: 10.18502/ijrm.v20i3.10712 -
IDCases 2021species, specifically (), are commonly associated with genitourinary (GU) tract infectious syndromes. However, spp. can also be involved in extragenital infections,...
species, specifically (), are commonly associated with genitourinary (GU) tract infectious syndromes. However, spp. can also be involved in extragenital infections, primarily in immunosuppressed patients. A 61 year old female was successfully treated with moxifloxacin and doxycycline combination therapy for an infected hematoma secondary to following a renal transplant. Microbiology technologists noted the growth of pinpoint, translucent non-hemolytic colonies, but no organisms seen on Gram stain. These findings prompted the updated culture report of, "Growth on culture plates, gram stain suggestive of organism lacking cell wall." Empiric antimicrobials were initiated to cover both spp. and spp before resulting . Initiating empiric therapy directed against spp. following Gram stain results and before organism speciation may prevent a lapse in effective therapy. This is especially important as perioperative antimicrobial prophylaxis regimens consist of beta-lactam regimens directed against common GI and GU pathogens, which lack activity against spp.
PubMed: 34189039
DOI: 10.1016/j.idcr.2021.e01190 -
Scientific Reports Jul 2021Female and male infertility have been associated to Chlamydia trachomatis, Ureaplasma spp. and Mycoplasma hominis urogenital infections. However, evidence from large...
Results from a large cross-sectional study assessing Chlamydia trachomatis, Ureaplasma spp. and Mycoplasma hominis urogenital infections in patients with primary infertility.
Female and male infertility have been associated to Chlamydia trachomatis, Ureaplasma spp. and Mycoplasma hominis urogenital infections. However, evidence from large studies assessing their prevalence and putative associations in patients with infertility is still scarce. The study design was a cross-sectional study including 5464 patients with a recent diagnosis of couple's primary infertility and 404 healthy control individuals from Cordoba, Argentina. Overall, the prevalence of C. trachomatis, Ureaplasma spp. and M. hominis urogenital infection was significantly higher in patients than in control individuals (5.3%, 22.8% and 7.4% vs. 2.0%, 17.8% and 1.7%, respectively). C. trachomatis and M. hominis infections were significantly more prevalent in male patients whereas Ureaplasma spp. and M. hominis infections were more prevalent in female patients. Of clinical importance, C. trachomatis and Ureaplasma spp. infections were significantly higher in patients younger than 25 years. Moreover, Ureaplasma spp. and M. hominis infections were associated to each other in either female or male patients being reciprocal risk factors of their co-infection. Our data revealed that C. trachomatis, Ureaplasma spp. and M. hominis are prevalent uropathogens in patients with couple's primary infertility. These results highlight the importance of including the screening of urogenital infections in the diagnostic workup of infertility.
Topics: Adult; Chlamydia Infections; Chlamydia trachomatis; Cross-Sectional Studies; Female; Humans; Infertility, Female; Infertility, Male; Male; Middle Aged; Mycoplasma Infections; Mycoplasma hominis; Ureaplasma; Ureaplasma Infections
PubMed: 34211075
DOI: 10.1038/s41598-021-93318-1 -
Systematic and Applied Microbiology Jan 2022Since 2006, a Mycoplasma species unidentifiable to the species level has been regularly isolated from the semen and prepuce of apparently healthy bulls, and occasionally...
Since 2006, a Mycoplasma species unidentifiable to the species level has been regularly isolated from the semen and prepuce of apparently healthy bulls, and occasionally from cattle displaying inflammatory disease of the genital tract. Seven of these Mycoplasma isolates were subjected to a comprehensive taxonomic study. The strains investigated grew well in modified Hayflick's medium and colonies on agar exhibited typical fried egg morphology and produced 'film and spots'. Transmission electron microscopy revealed a cell morphology characteristic of mycoplasmas with spherically shaped cells bounded by a bi-layered cell membrane. The strains studied neither produced acid from sugar carbon sources nor did hydrolyse arginine or urea, and genome annotation indicated that organic acids (pyruvate, lactate) are used as energy sources. Phylogenetic analyses of 16S rRNA gene sequences, the 16S-23S intergenic spacer region, and partial rpoB gene and protein sequences placed the strains within the Mycoplasma (M.) bovis cluster of the Hominis group with M. primatum, M. agalactiae, and M. bovis being their closest relatives. Genomic information including whole-genome similarity metrics (ANIb, ANIm, TETRA, dDDH, AAI) and phylogenomics, proteomic features revealed by matrix-assisted laser desorption ionization time of flight (MALDI-ToF) mass spectrometry as well as serological reactions and polar lipid profiling strongly indicated that the strains examined were representatives of a hitherto unclassified species of genus Mycoplasma, for which the name Mycoplasma tauri sp. nov. with type strain Zaradi2 (=ATCC BAA-1891 = DSM 22451) is proposed.
Topics: Animals; Bacterial Typing Techniques; Cattle; DNA, Bacterial; Genitalia; Male; Mycoplasma; Phylogeny; Proteomics; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 34990977
DOI: 10.1016/j.syapm.2021.126292 -
Infection and Drug Resistance 2022meningitis is a rare postoperative complication of neurosurgery. Accurate and early diagnosis of remains challenging because of the limitations of traditional...
BACKGROUND
meningitis is a rare postoperative complication of neurosurgery. Accurate and early diagnosis of remains challenging because of the limitations of traditional detection methods. Metagenomic next-generation sequencing (mNGS) is an advanced technique with high sensitivity and specificity for identifying infectious pathogens; however, its application in diagnosing meningitis has not been widely studied.
CASE PRESENTATION
We report the case of a 61-year-old man who presented with fever and headache after neurosurgical treatment for a cerebral hemorrhage. Empiric antibiotic therapy was ineffective. Traditional culture of pathogens and serological testing yielded negative results, but was detected in the cerebrospinal fluid by mNGS. After further verification by polymerase chain reaction (PCR), the patient's clinical treatment was adjusted accordingly. With targeted antibiotic intervention, the patient's symptoms were effectively alleviated, and clinical indicators returned to normal levels. Furthermore, the abundance of decreased significantly compared to the initial mNGS reading after targeted treatment, indicating that the infection caused by was effectively controlled.
CONCLUSION
Using mNGS, we found that may be a candidate causative agent of meningitis. The technique also has the advantage of timeliness and accuracy that traditional cultures cannot achieve. A combination of mNGS with PCR is recommended to identify pathogens in the early stages of infectious diseases to administer targeted clinical medication.
PubMed: 35983300
DOI: 10.2147/IDR.S371771 -
Journal of Clinical Laboratory Analysis Apr 2020The aim of this study was to investigate the infection and antimicrobial resistance of Ureaplasma urealyticum and Mycoplasma hominis in patients with genitourinary...
BACKGROUND
The aim of this study was to investigate the infection and antimicrobial resistance of Ureaplasma urealyticum and Mycoplasma hominis in patients with genitourinary symptoms among Hakka population in Meizhou, China.
METHODS
A total of 12 633 females and 3315 males who presented urogenital symptoms and were subjected to mycoplasma tests from 2014 to 2018 were enrolled in this study. The mycoplasma detection and antimicrobial susceptibility were tested using the Mycoplasma ID/AST kit.
RESULTS
The total incidence of mycoplasma infection, as well as the incidence of U urealyticum in Hakka population was annually increasing from 2014 to 2018. The total incidences and U urealyticum infection were more prevalent in females than males. Higher positive rate of mycoplasmas infection was observed in women aged 16-20 (50.9%) and men aged 26-30 (25.4%). The occurrence of antimicrobial resistance of mycoplasma to antibacterial agents remained relatively similar in the past five years. Ureaplasma urealyticum infection, M hominis infection, and co-infection of resistance to levofloxacin, erythromycin, ciprofloxacin, ofloxacin, roxithromycin, azithromycin, clarithromycin, and sparfloxacin were dramatically higher in females than in males.
CONCLUSION
Our findings indicate a high burden of mycoplasmas infection and antimicrobial resistance of mycoplasmas infection among females, and josamycin and minocycline may be recommended as the primary choice in clinical treatment of anti-mycoplasmas.
Topics: Adolescent; Adult; Age Distribution; Anti-Bacterial Agents; China; Drug Resistance, Bacterial; Female; Humans; Incidence; Male; Microbial Sensitivity Tests; Middle Aged; Mycoplasma Infections; Mycoplasma hominis; Prevalence; Reproductive Tract Infections; Ureaplasma Infections; Ureaplasma urealyticum; Young Adult
PubMed: 31721313
DOI: 10.1002/jcla.23118 -
Journal of Clinical Laboratory Analysis Apr 2022Mycoplasma hominis is the smallest prokaryotic microorganism with no cell wall, high pleomorphism, and slower reproduction than bacteria. It is difficult for clinical...
BACKGROUND
Mycoplasma hominis is the smallest prokaryotic microorganism with no cell wall, high pleomorphism, and slower reproduction than bacteria. It is difficult for clinical technicians to find M. hominis through the negative Gram staining of specimens. Therefore, it is likely to miss detection in routine clinical smear etiological examination. M. hominis is generally considered to be a common colonizing bacterium in urogenital tract with low pathogenicity, and it is usually difficult to invade submucosal tissue and enter the bloodstream.
METHODS
The abscesses of the patient were examined histopathologically, and the pus in the abscesses was extracted for etiological examination. MALDI-TOF MS was used to identify and confirmed the pathogens in the specimens. The commercial Mycoplasma isolation, culture, and drug sensitivity kit was used to determine antibiotic susceptibility.
RESULTS
No pathogens were found after pathological and smear microscopic examination of the puncture fluid from the sacrococcygeal and pelvic abscesses. Until 48 h later, small, translucent, and gray-white colonies were observed in the blood plate culture results. The laboratory physician ultimately determined that the pathogen was M. hominis by MALDI-TOF MS.
CONCLUSION
We report a case of extra-urogenital cystic abscesses infected by M. hominis, in order to improve clinicians' comprehensive understanding of the pathogenicity of Mycoplasma. In addition, the clinical laboratory technician should pay attention to the role of Wright-Giemsa staining of puncture fluid smear in the preliminary detection and the application of MALDI-TOF MS in identification of uncommon pathogenic microorganisms.
Topics: Abscess; Bacteria; Blood Culture; Humans; Mycoplasma hominis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 35285086
DOI: 10.1002/jcla.24329 -
European Annals of Otorhinolaryngology,... Jan 2020Beside HPV infection, there is currently no evidence of association between head and neck squamous cell carcinomas and microbial infections. We report the case of a...
INTRODUCTION
Beside HPV infection, there is currently no evidence of association between head and neck squamous cell carcinomas and microbial infections. We report the case of a cervical squamous cell carcinoma by Mycoplasma hominis.
CASE SUMMARY
A 20-year-old woman, consulted for a swelling on the left cervical side. Clinical examination found a large fixed mass. Biological tests found no evidence of infection. Biopsies of the cervical lesion diagnosed an HPV negative squamous cell carcinoma. Microbiological tests of 16sRNA identification showed the presence of Mycoplasma hominis in the 3 specimens. The patient was treated by induction chemotherapy associated to antibiotherapy, followed by chemo-radiotherapy.
DISCUSSION
The present case suggests that oropharyngeal infection by Mycoplasma hominis might be more frequent than expected, that 16sRNA is an efficient technique to isolate this pathogen and finally that further studies are required to document its potential oncogenic role in head and neck squamous cell carcinomas.
Topics: Female; Head and Neck Neoplasms; Humans; Mycoplasma Infections; Mycoplasma hominis; Neoplasms, Unknown Primary; Squamous Cell Carcinoma of Head and Neck; Young Adult
PubMed: 31186167
DOI: 10.1016/j.anorl.2019.05.020 -
Frontiers in Microbiology 2019Mycoplasmas represent important etiologic agents of many human diseases. Due to increasing antimicrobial resistance and slow rate of novel discovery, unconventional...
RATIONALE
Mycoplasmas represent important etiologic agents of many human diseases. Due to increasing antimicrobial resistance and slow rate of novel discovery, unconventional methods of drug discovery are necessary. Copper ions are utilized in host microbial killing, and bacteria must regulate intracellular Cu concentrations to avoid toxicity. We hypothesized that human mollicutes may have susceptibility to Cu-induced toxicity, and compounds that augment copper-dependent killing.
METHODS
(Mpn), (Up), (Uu), and (Mh) were exposed to CuSO to determine minimal inhibitory concentrations (MICs). Once inhibitory concentrations had been determined, bacteria were treated with an FDA-approved drug disulfiram (DSF), glyoxal bis(4-methyl-3-thiosemicarbazone) (GTSM), and 2,9-dimethyl-1,10-phenanthroline (neocuproine), with or without Cu, to determine compound MICs.
RESULTS
species and Mh were able to tolerate 30-60 μM CuSO, while Mpn tolerated over 10-fold higher concentrations (>1 mM). GTSM inhibited growth of all four organisms, but was unaffected by Cu addition. Inhibition by GTSM was reduced by addition of the cell-impermeant Cu chelator, bathocuproine disulfonate (BCS). Neocuproine exhibited Cu-dependent growth inhibition of all organisms. DSF exhibited Cu-dependent growth inhibition against Mh at low micromolar concentrations, and at intermediate concentrations for Mpn.
CONCLUSION
MICs for CuSO differ widely among human mollicutes, with higher MICs for Mpn compared to Mh, Uu, and Up. DSF and Neocuproine exhibit Cu-dependent inhibition of mollicutes with copper concentrations between 25 and 50 μM. GTSM has copper-dependent anti-microbial activity at low levels of copper. Drug enhanced copper toxicity is a promising avenue for novel therapeutic development research with and species.
PubMed: 31417517
DOI: 10.3389/fmicb.2019.01720 -
Microorganisms Apr 2023causes trichomoniasis, the most recurrent sexually transmitted infection (STI) worldwide. Genital mycoplasmas, not considered STI agents, are frequently isolated from...
causes trichomoniasis, the most recurrent sexually transmitted infection (STI) worldwide. Genital mycoplasmas, not considered STI agents, are frequently isolated from the female genital tract. A symbiosis between species and has been described. The aim of this study was to conduct molecular-based analyses of vaginal specimens, thus assessing the prevalence of non-STI infections. In total, 582 samples from female patients and an additional 20 isolates were analyzed by PCR using specific 16S rRNA primers, and the obtained PCR products were sequenced. species were detected in 28.2% of the collected vaginal samples. was found in 21.5% of the specimens, species were found in 7.5% of the samples. The molecular data of the newly described species, , were obtained for the first time in Austria, in a sample also positive for . Analyses of the cultivated strains confirmed the presence of in two out of 20 samples. A comparably high prevalence of genital mycoplasmas was revealed through advanced diagnostic assays, with and being the most prevalent species. The previously described symbiotic relationship between and was confirmed.
PubMed: 37110356
DOI: 10.3390/microorganisms11040933