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Open Forum Infectious Diseases Jul 2019spp. are environmental bacterial organisms that rarely cause disease in humans. spp. infections are infrequently reported in the literature, and infections are quite... (Review)
Review
spp. are environmental bacterial organisms that rarely cause disease in humans. spp. infections are infrequently reported in the literature, and infections are quite uncommon. spp. usually infect immunocompromised hosts and can have highly resistant antibiotic susceptibility patterns. Here we report a case of bacteremia and severe cellulitis in a patient with cirrhosis and review the literature of other spp. infections.
PubMed: 31334299
DOI: 10.1093/ofid/ofz282 -
Journal of Clinical Microbiology May 2023Due to limited therapeutic options, there is a clinical need to assess the activity of the combination of aztreonam (ATM) and ceftazidime-avibactam (CZA) to guide the...
Due to limited therapeutic options, there is a clinical need to assess the activity of the combination of aztreonam (ATM) and ceftazidime-avibactam (CZA) to guide the therapeutic management of multidrug-resistant (MDR) Gram-negative organism infections. We set out to develop a practical MIC-based broth disk elution (BDE) method to determine the activity of the combination ATM-CZA using readily available supplies and compare it to reference broth microdilution (BMD). For the BDE method, a 30-μg ATM disk, a 30/20-μg CZA disk, both disks in combination, and no disks were added to 4 separate 5-mL cation-adjusted Mueller-Hinton broth (CA-MHB) tubes, using various manufacturers. Three testing sites performed both BDE and reference BMD testing of bacterial isolates in parallel from a single 0.5 McFarland standard inoculum and after overnight incubation, assessed them for growth (not susceptible) or no growth (susceptible) at a final concentration of 6/6/4 μg/mL ATM-CZA. During the first phase, the precision and accuracy of the BDE were analyzed by testing 61 isolates at all sites. This testing yielded 98.3% precision between sites, with 98.3% categorical agreement and 1.8% major errors (ME). During the second phase, at each site, we evaluated unique, clinical isolates of metallo-β-lactamase (MBL)-producing ( = 75), carbapenem-resistant Pseudomonas aeruginosa ( = 25), Stenotrophomonas maltophilia ( = 46), and sp. ( = 1). This testing resulted in 97.9% categorical agreement, with 2.4% ME. Different results were observed for different disk and CA-MHB manufacturers, requiring a supplemental ATM-CZA-not-susceptible quality control organism to ensure the accuracy of results. The BDE is a precise and effective methodology for determining susceptibility to the combination ATM-CZA.
Topics: Humans; Aztreonam; Anti-Bacterial Agents; Microbial Sensitivity Tests; Ceftazidime; Drug Combinations; Gram-Negative Bacteria; Pseudomonas aeruginosa; beta-Lactamases
PubMed: 37070979
DOI: 10.1128/jcm.01647-22 -
Food Science & Nutrition Feb 2023The aim of this study was to investigate the sensory indicators, biogenic amine contents, and bacterial community structure and diversity of chilled chicken stored at... (Review)
Review
The aim of this study was to investigate the sensory indicators, biogenic amine contents, and bacterial community structure and diversity of chilled chicken stored at 4°C under aerobic conditions. Bacterial diversity and dominant bacteria were analyzed using high-throughput sequencing technique (HTS). The relationship between biogenic amine contents and microbial community structure was studied. The results showed that contents of putrescine and cadaverine increased significantly with storage time. Proteobacteria was absolutely dominant flora at the phylum level. The predominant spoilage bacteria found in chicken thighs were , , , , and , and the difference with chicken breasts was related to the presence of and absence of . , , and were reported for the first time as an important contributor to the spoilage-related microflora Bacterial diversity and richness indices showed fluctuating and decreasing trend with storage time. The redundancy analysis showed that the relative abundance of , , and was positively related to the contents of putrescine, cadaverine, and tyramine, while and showed positive relationship with putrescine content. Furthermore, positive relationship of and with the contents of cadaverine and tyramine was proposed for the first time. The key findings of this study can provide experimental data for food safety monitoring during refrigerated storage and preservation for poultry meat products.
PubMed: 36789075
DOI: 10.1002/fsn3.3122 -
IDCases 2021Formerly classified under the genus species are common gram-negative, environmental bacterium ubiquitous in soil and water. While infrequent, infections of human hosts...
Formerly classified under the genus species are common gram-negative, environmental bacterium ubiquitous in soil and water. While infrequent, infections of human hosts can result in devastating consequences due the bacteria's intrinsic multidrug resistance, particularly in those who are immunocompromised. The pathogenicity and mechanisms for resistance remain poorly understood at this time. The case presented in this report details bacteremia secondary to a soft tissue infection of the lower extremities and adds to the 60 documented infections to date, of which 15 were also characterized by a similar infection.
PubMed: 33850715
DOI: 10.1016/j.idcr.2021.e01061 -
Japanese Journal of Infectious Diseases Nov 2023Myroides species have recently been reported more frequently in outbreaks in clinics and intensive care units (ICUs). In this study, we aimed to investigate the epidemic...
Myroides species have recently been reported more frequently in outbreaks in clinics and intensive care units (ICUs). In this study, we aimed to investigate the epidemic potential, antibiotic resistance profile, and risk factors of M. odoratimimus isolates that are increasingly being isolated from the ICUs of our hospital. Data from patients whose Myroides spp. were isolated from their clinical specimens over a 5-year period (September 2016 to January 2022) were retrospectively analyzed. Bacterial identification was performed using a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The presence of antibiotic resistance genes was analyzed using polymerase chain reaction (PCR). Possible clonal associations between isolates were investigated using enterobacterial repetitive intergenic consensus (ERIC)-PCR. As a result, 66 isolates were identified as M. odoratimimus and one isolate was identified as M. odoratus. The bla resistance gene was detected in all M. odoratimimus isolates, whereas sul2 was detected in ten isolates and tetX was detected in 11 isolates. No other resistance genes, such as bla, were detected. Additionally, two different clonal association patterns were discovered in the 24 selected isolates through the ERIC-PCR method. The increase in the immunosuppressive patient population indicate the possibility of encountering this agent and other opportunistic pathogens more frequently in the future.
Topics: Humans; Persistent Infection; Retrospective Studies; Enterobacteriaceae; Drug Resistance, Multiple, Bacterial; Anti-Bacterial Agents; Disease Outbreaks; Hospitals; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 37394461
DOI: 10.7883/yoken.JJID.2023.005 -
Indian Journal of Critical Care... Apr 2024species are ubiquitous in the environment and cause a variety of infections like urinary tract infections (UTI), sepsis, meningitis, cholecystitis, pneumonia, and soft...
AIM AND BACKGROUND
species are ubiquitous in the environment and cause a variety of infections like urinary tract infections (UTI), sepsis, meningitis, cholecystitis, pneumonia, and soft tissue infections, especially among immunocompromised populations. These are usually resistant to multiple antibiotics. This study aimed to demonstrate the clinical profile, underlying comorbidities, and antimicrobial susceptibility of isolates obtained from nosocomial UTI cases.
MATERIALS AND METHODS
A sudden rise in the isolation of spp. from the repeated urine samples of admitted patients alerted us to conduct this retrospective observational study. Urine cultures that grew species were included in this study. Antibiotic susceptibility was performed and the patient's clinical data was analyzed.
RESULTS
A total of 14 spp. isolates were obtained from urine culture. The maximum number of cases (71.4%) were from the Nephrology ward and ICUs. The average (mean) age of patients was 46 years (range 2-80 years). All patients were catheterized. All isolates were multidrug resistant. Minocycline and doxycycline were the only drugs found effective in this study.
CONCLUSIONS
species are emerging rare pathogens that can cause UTI in immunocompromised and catheterized patients. Minocycline may be used for treating such infections.
HOW TO CITE THIS ARTICLE
Sahu C, Chaudhary R, Bhartiya C, Patel SS, Bhatnagar N. A Retrospective Study on UTI by Species: An Emerging Drug Resistant Nosocomial Pathogen. Indian J Crit Care Med 2024;28(4):399-403.
PubMed: 38585315
DOI: 10.5005/jp-journals-10071-24683 -
Access Microbiology 2023Infections forby spp. can lead to significant morbidity and mortality, particularly in immunocompromised patients with underlying co-morbidities. Recent reports have...
INTRODUCTION
Infections forby spp. can lead to significant morbidity and mortality, particularly in immunocompromised patients with underlying co-morbidities. Recent reports have highlighted its intrinsic and acquired drug resistance, making it a particularly challenging infectious agent to combat.
METHODS
spp. isolated and reported in clinically significant urine samples were considered for the study. Identification of the organism was done via the VITEK 2C system. Antibiotic susceptibility testing was done using both manual and automated methods following Clinical and Laboratory Standards Institute (CLSI) guidelines. Existing literature was searched on MEDLINE using PubMed.
RESULTS
We present a series of five catheter-associated urinary tract infections due to , with sensitivity to only minocycline. This is the first case from Western India, and the third case in the existing literature that shows sensitivity only to minocycline. Our literature review is the first to systematically describe contributory factors to infection, allowing us to devise a clinically relevant tool that delineates contributory factors and efficacious drugs in spp infection.
CONCLUSION
spp infections, previously considered rare and opportunistic, need cognizance and diagnostic suspicion especially in particular associated conditions.
PubMed: 37323940
DOI: 10.1099/acmi.0.000549.v2 -
Frontiers in Microbiology 2022In this study, we identified and characterized a novel chromosomally-encoded class B metallo-β-lactamase (MBL) gene designated in a carbapenem-resistant isolate P34...
In this study, we identified and characterized a novel chromosomally-encoded class B metallo-β-lactamase (MBL) gene designated in a carbapenem-resistant isolate P34 isolated from sewage discharged from an animal farm. Comparative analysis of the deduced amino acid sequence revealed that WUS-1 shares the highest amino acid similarities with the function-characterized MBLs MUS-1 (AAN63647.1; 70.73%) and TUS-1 (AAN63648.1; 70.32%). The recombinant carrying exhibited increased MICs levels against a number of β-lactam antimicrobials such as carbenicillin, ampicillin and imipenem, and β-lactamase inhibitors (clavulanic acid and tazobactam). The metallo-β-lactamase WUS-1 could also hydrolyze these antimicrobials and the hydrolytic activities could be inhibited by EDTA. Genetic context analysis of revealed that no mobile genetic element was found in its surrounding region. The plasmid pMA84474 of P34 harbored 6 resistance genes ( , , , , and ) within an approximately 17 kb multidrug resistance (MDR) region. These genes, however, were all related to mobile genetic elements.
PubMed: 36532482
DOI: 10.3389/fmicb.2022.1059997 -
IDCases 2019spp., formerly ., are gram-negative, non-motile, traditionally opportunistic pathogens that are infrequent clinical isolates. spp. have been pathogenically implicated...
spp., formerly ., are gram-negative, non-motile, traditionally opportunistic pathogens that are infrequent clinical isolates. spp. have been pathogenically implicated in only 52 reported cases since the discovery of the genus in the 1920s. We present a case of spp. lower extremity cellulitis and bacteremia. To our knowledge, this is the 16th case of cutaneous infection caused by spp. Etiology of this patient's infection was felt to be related to exposure of his pre-existing lower extremity wounds to soil and water versus a dog lick in the context of relative immunosuppression from type 2 diabetes and chronic inhaled steroid use. Given the characteristic multi-drug resistance of spp., resistance to usual empiric antimicrobials given for cellulitis, and potential for fatal infection in cases of pan-resistance, it is important that clinicians remain alert to the possibility of this rare pathogen.
PubMed: 31538047
DOI: 10.1016/j.idcr.2019.e00638 -
Microorganisms Mar 2022Plant-growth-promoting endophytes (PGPE) are a kind of beneficial microorganisms which could inhabit plant tissues to antagonize certain plant pathogens and promote the...
Plant-growth-promoting endophytes (PGPE) are a kind of beneficial microorganisms which could inhabit plant tissues to antagonize certain plant pathogens and promote the host plant's growth and development. At present, many studies have confirmed the mutualistic effect of endophytes with plants, but there are few systematic studies on beneficial roles between endophytes and , especially on the PGPE characteristics of the forage and environmental restoration plant. This study aimed to isolate PGPE from , evaluate their effects on plant growth, and ultimately acquire desirable microbial inoculants for agricultural use. First, endophytes were isolated from by plant re-inoculation experiment, and identified by morphological and molecular analyses. Fixation medium and methods were carried out to assess the nitrogen fixation ability of the strains. Then, the ability to dissolve phosphorus was determined by the Olsen and silicate medium methods; secretory IAA was measured by Salkowski colorimetric method; and the inhibitive effects on phytopathogen were observed by confrontation culture. Twenty-one strains were isolated from four varieties of , among which 14 strains with plant-growth-promoting characteristics were obtained by re-inoculation experiments, including seven endophytic bacteria and seven endophytic fungi. Further evaluation of three selected strains with the most significant PGP attributes were performed by using the pot re-inoculation experiment which revealed that TG2-B derived from was the most effective plant-growth-promoting agent due to its ability to produce high levels of IAA; the strain exhibited the most robust ability in dissolving inorganic phosphorus; and manifested a conspicuously antagonistic effect against a variety of plant pathogens. For the first time, this study reports the identification of endophytes that were able to promote plant growth and have a considerable antagonistic effects on plant pathogens, which could be considered as microbial inoculants for using in improving crop production and plant disease control.
PubMed: 35456782
DOI: 10.3390/microorganisms10040731