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Materials (Basel, Switzerland) Mar 2022The synthesized glass system with a composition of (80-x) TeO2-10P2O5-10Nb2O5-xKCl mol% (where x = 5, 10, 15, 20, and 25) was successfully fabricated. The density (ρ)...
The synthesized glass system with a composition of (80-x) TeO2-10P2O5-10Nb2O5-xKCl mol% (where x = 5, 10, 15, 20, and 25) was successfully fabricated. The density (ρ) and molar volume (Vm) have been calculated. The investigated glasses were characterized using different analysis methods (differential thermal analysis (DTA) and UV-VIS-NIR spectroscopy). The radiation shielding effectiveness of the synthesized glass system was evaluated using different shielding parameters, such as mass and linear attenuation coefficients (MAC, LAC), half-value layer (HVL), mean free path (MFP), effective atomic number (Zeff), and effective electron number (Neff). The results showed that with the increasing potassium chloride (KCl) concentration and decreasing tellurium oxide (TeO2) concentration, the density, refractive index, Urbach energy (Eu), and glass transition temperature (Tg) decreased, while the optical energy gap (Eopt) and thermal stability increased. As the KCl concentration increases, the values of MAC, LAC, and Zeff increase in the following order: TPNK5 % > TPNK10 % > TPNK15 % > TPNK20 % > TPNK25 %. Additionally, the shielding effectiveness of TPNK glass system showed good performance compared with some standard materials. The synthesized glass with a minimum KCl content has both good shielding effectiveness and good optical properties, in addition to reasonable thermal stability, which makes it suitable for shielding and optical applications.
PubMed: 35407736
DOI: 10.3390/ma15072403 -
Bioscience Reports Nov 2022Hypertension affects 30% of adults and is the leading risk factor for cardiovascular disease. Kidney sodium reabsorption plays a vital role in the initial stage and... (Review)
Review
Hypertension affects 30% of adults and is the leading risk factor for cardiovascular disease. Kidney sodium reabsorption plays a vital role in the initial stage and development of essential hypertension. It has been extensively reported that the variants of kidney ion handling genes are associated to blood pressure, and clinical features of hypertension. However, the underlying mechanisms by which these variants alter protein function are rarely summarized. In addition, the variation of one single gene is often limited to induce a significant effect on blood pressure. In the past few decades, the influence by genes × genes (G × G) and/or genotype × environment (G × E) interactions on a given trait, for example, blood pressure, have been widely considered, especially in studies on polygenic genetic traits. In the present review, we discuss the progress in genetics studies on kidney ion handling genes, encoding Na+ channels (Na+-Cl- cotransporter [NCC], Na-K-2Cl cotransporter [NKCC2], epithelial Na+ channels [ENaCs]), K+ channel (renal outer medullary potassium channel [ROMK]), and Cl- channels (Pendrin, chloride voltage-gated channel Kb [CLC-Kb]), respectively, and their upstream kinases, WNKs and SGK1. We seek to clarify how these genes are involved in kidney sodium absorption and influence blood pressure, especially emphasizing the underlying mechanisms by which genetic variants alter protein functions and interaction in blood pressure regulation. The present review aims to enhance our understanding of the important role of kidney ion handling genes/channels in blood pressure control.
Topics: Adult; Humans; Blood Pressure; Kidney; Hypertension; Sodium; Sodium-Potassium-Chloride Symporters; Solute Carrier Family 12, Member 3
PubMed: 36305246
DOI: 10.1042/BSR20220977 -
Circulation. Heart Failure Nov 2021Animal models implicate FGF-23 (fibroblast growth factor-23) as a direct contributor to adverse cardiorenal interactions such as sodium avidity, diuretic resistance, and...
BACKGROUND
Animal models implicate FGF-23 (fibroblast growth factor-23) as a direct contributor to adverse cardiorenal interactions such as sodium avidity, diuretic resistance, and neurohormonal activation, but this has not been conclusively demonstrated in humans. Therefore, we aimed to evaluate whether FGF-23 is associated with parameters of cardiorenal dysfunction in humans with heart failure, independent of confounding factors.
METHODS
One hundred ninety-nine outpatients with heart failure undergoing diuretic treatment at the Yale Transitional Care Center were enrolled and underwent blood collection, and urine sampling before and after diuretics.
RESULTS
FGF-23 was associated with several metrics of disease severity such as higher home loop diuretic dose and NT-proBNP (N-terminal pro-B-type natriuretic peptide), and lower estimated glomerular filtration rate, serum chloride, and serum albumin. Multivariable analysis demonstrated no statistically significant association between FGF-23 and sodium avidity measured by fractional excretion of sodium, or proximal or distal tubular sodium reabsorption, either before diuretic administration or at peak diuresis (≥0.11 for all). Likewise, FGF-23 was not independently associated with parameters of diuretic resistance (diuretic excretion, cumulative urine and sodium output, and loop diuretic efficiency [≥0.33 for all]) or neurohormonal activation (plasma or urine renin [≥0.36 for all]). Moreover, the upper boundary of the 95% CI of all the partial correlations were ≤0.30, supporting the lack of meaningful correlations. FGF-23 was not associated with mortality in multivariable analysis (=0.44).
CONCLUSIONS
FGF-23 was not meaningfully associated with any cardiorenal parameter in patients with heart failure. While our methods cannot rule out a small effect, FGF-23 is unlikely to be a primary driver of cardiorenal interactions.
Topics: Aged; Aged, 80 and over; Diuresis; Diuretics; Female; Fibroblast Growth Factor-23; Heart Failure; Humans; Male; Middle Aged; Renin; Sodium; Sodium Potassium Chloride Symporter Inhibitors
PubMed: 34689571
DOI: 10.1161/CIRCHEARTFAILURE.121.008385 -
Molecules (Basel, Switzerland) Sep 2023Sodium chloride (NaCl) is a commonly used additive in minimally processed fish-based products. The addition of NaCl to fish products and packaging in a modified...
Sodium chloride (NaCl) is a commonly used additive in minimally processed fish-based products. The addition of NaCl to fish products and packaging in a modified atmosphere is usually efficient with regard to limiting the occurrence of the aquatic environmental pathogen Given the negative effects of excess NaCl in the diet, there is a growing demand to reduce NaCl in food products with safer substituents, but the knowledge of their impact on antibiotic resistant is limited. This study aimed to evaluate the physiological and transcriptome characteristics of NT06 isolated from fish and to determine the effect of selected concentrations of alternative NaCl compounds (KCl/NaL/NaC) on the NT06 virulence phenotype and genotype. In the study, among the isolated microorganisms, NT06 showed the highest antibiotic resistance (to ampicillin, ceftriaxone, nalidixic acid, and norfloxacin) and the ability to grow at 4 °C. The Comprehensive Antibiotic Resistance Database (CARD) and the Virulence Factor Database (VFDB) revealed the presence of 24 and 134 gene products assigned to AMR and VF in the NT06 transcriptome, respectively. KCl, KCl/NaL and KCl/NaL/NaC inhibited pyocyanin biosynthesis, elastase activity, and protease activity from 40 to 77%. The above virulence phenotypic observations were confirmed via RT-qPCR analyses, which showed that all tested AMR and VF genes were the most downregulated due to KCl/NaL/NaC treatment. In conclusion, this study provides insight into the potential AMR and VF among foodborne and the possible impairment of those features by KCl, NaL, and NaC, which exert synergistic effects and can be used in minimally processed fish-based products.
Topics: Animals; Virulence; Anti-Bacterial Agents; Pseudomonas aeruginosa; Sodium Citrate; Sodium Lactate; Potassium Chloride; Sodium Chloride; Drug Resistance, Bacterial; Virulence Factors; Pseudomonas Infections
PubMed: 37764430
DOI: 10.3390/molecules28186654 -
Current Biology : CB Mar 2022Central pacemaker neurons regulate circadian rhythms and undergo diurnal variation in electrical activity in mammals and flies. Circadian variation in the intracellular...
Central pacemaker neurons regulate circadian rhythms and undergo diurnal variation in electrical activity in mammals and flies. Circadian variation in the intracellular chloride concentration of mammalian pacemaker neurons has been proposed to influence the response to GABAergic neurotransmission through GABA receptor chloride channels. However, results have been contradictory, and a recent study demonstrated circadian variation in pacemaker neuron chloride without an effect on GABA response. Therefore, whether and how intracellular chloride regulates circadian rhythms remains controversial. Here, we demonstrate a signaling role for intracellular chloride in the Drosophila small ventral lateral (sLN) pacemaker neurons. In control flies, intracellular chloride increases in sLNs over the course of the morning. Chloride transport through sodium-potassium-2-chloride (NKCC) and potassium-chloride (KCC) cotransporters is a major determinant of intracellular chloride concentrations.Drosophila melanogaster with loss-of-function mutations in the NKCC encoded by Ncc69 have abnormally low intracellular chloride 6 h after lights on, loss of morning anticipation, and a prolonged circadian period. Loss of kcc, which is expected to increase intracellular chloride, suppresses the long-period phenotype of Ncc69 mutant flies. Activation of a chloride-inhibited kinase cascade, consisting of WNK (with no lysine [K]) kinase and its downstream substrate, Fray, is necessary and sufficient to prolong period length. Fray activation of an inwardly rectifying potassium channel, Irk1, is also required for the long-period phenotype. These results indicate that the NKCC-dependent rise in intracellular chloride in Drosophila sLN pacemakers restrains WNK-Fray signaling and overactivation of an inwardly rectifying potassium channel to maintain normal circadian period length.
Topics: Animals; Chlorides; Circadian Rhythm; Drosophila Proteins; Drosophila melanogaster; Mammals; Neurons; Protein Serine-Threonine Kinases; Signal Transduction
PubMed: 35303418
DOI: 10.1016/j.cub.2022.03.017 -
International Journal of Molecular... Sep 2020To ameliorate ischemia-induced graft injury, optimal organ preservation remains a critical hallmark event in solid organ transplantation. Although numerous preservation... (Review)
Review
To ameliorate ischemia-induced graft injury, optimal organ preservation remains a critical hallmark event in solid organ transplantation. Although numerous preservation solutions are in use, they still have functional limitations. Here, we present a concise review of a modified Histidine-Tryptophan-Ketoglutarate (HTK) solution, named HTK-N. Its composition differs from standard HTK solution, carrying larger antioxidative capacity and providing inherent toxicity as well as improved tolerance to cold aiming to attenuate cold storage injury in organ transplantation. The amino acids glycine, alanine and arginine were supplemented, N-acetyl-histidine partially replaced histidine, and aspartate and lactobionate substituted chloride. Several in vitro studies confirmed the superiority of HTK-N in comparison to HTK, being tested in vivo in animal models for liver, kidney, pancreas, small bowel, heart and lung transplantation to adjust ingredients for required conditions, as well as to determine its innocuousness, applicability and potential advantages. HTK-N solution has proven to be advantageous especially in the preservation of liver and heart grafts in vivo and in vitro. Thus, ongoing clinical trials and further studies in large animal models and consequently in humans are inevitable to show its ability minimizing ischemia-induced graft injury in the sequel of organ transplantation.
Topics: Alanine; Animals; Arginine; Cryopreservation; Glucose; Glycine; Humans; Liver; Mannitol; Organ Preservation; Organ Preservation Solutions; Organ Transplantation; Pancreas; Potassium Chloride; Procaine; Reperfusion Injury
PubMed: 32899772
DOI: 10.3390/ijms21186468 -
International Journal of Gastronomy and... Mar 2022High sodium intakes represent an important risk factor for hypertension, cardiovascular diseases and kidney diseases. Even during the current COVID-19 pandemic,...
High sodium intakes represent an important risk factor for hypertension, cardiovascular diseases and kidney diseases. Even during the current COVID-19 pandemic, hypertension was related to higher mortality rate in patients with coronavirus. Thus, it is necessary to apply strategies to reduce or replace sodium content in food most widely consumed, like bread. This work aimed at determining the sensorial potassium threshold when potassium chloride is used as a sodium chloride replacer in bread formulation, and at analyzing the effects of such replacement on the properties of dough and on the technological and sensorial quality of bread. A decrease was observed in dough rheological properties with NaCl reduction in the formulation. Sensorial potassium threshold was determined and KCl was used in bread formulation as a NaCl replacement up to 0.92% of the regular salt content (2%) undetected by its characteristic taste. NaCl reduction resulted in bread with lower specific volume, higher firmness, faster staling and clearer crust. KCl bread showed similar technological to 2% NaCl bread. Finally, it was possible to replace 50% of NaCl with KCl without reducing quality and consumer acceptability.
PubMed: 36568859
DOI: 10.1016/j.ijgfs.2022.100486 -
Epilepsia Jul 2022A recent Phase II randomized, controlled trial of bumetanide as an adjunctive treatment for neonatal seizures showed a robust efficacy signal and no evidence of... (Randomized Controlled Trial)
Randomized Controlled Trial
A recent Phase II randomized, controlled trial of bumetanide as an adjunctive treatment for neonatal seizures showed a robust efficacy signal and no evidence of toxicity. Concerns regarding bumetanide as an adjunctive anticonvulsant are addressed here. An adequately powered multi-institutional trial is needed to accurately determine efficacy.
Topics: Bumetanide; Epilepsy; Humans; Infant, Newborn; Infant, Newborn, Diseases; Seizures; Sodium Potassium Chloride Symporter Inhibitors; Solute Carrier Family 12, Member 2
PubMed: 35524444
DOI: 10.1111/epi.17278 -
Current Therapeutic Research, Clinical... 2023Cilnidipine is a fourth-generation calcium channel blocker that is clinically used to treat hypertension. It is a dihydropyridine that blocks L- and N-type calcium...
BACKGROUND
Cilnidipine is a fourth-generation calcium channel blocker that is clinically used to treat hypertension. It is a dihydropyridine that blocks L- and N-type calcium channels. The inhibitory effect of cilnidipine on isolated detrusor muscle contractility has not been studied. This study investigated the inhibitory effect of cilnidipine on isolated caprine (goat) detrusor muscle contractility and the reversal of the inhibition by calcium channel openers.
METHODS
Fourteen caprine detrusor strips were made to contract using 80 mM potassium chloride before and after addition of three concentrations (20, 40, and 60 µM) of cilnidipine. Two reversal agents, the L-type calcium channel opener FPL64716, and the N-type calcium channel opener GV-58, were investigated for their ability to reverse the inhibitory effect of 40 µΜ cilnidipine on potassium chloride-induced detrusor contractility.
RESULTS
Cilnidipine caused a dose-dependent and statistically significant inhibition of detrusor contractility at all concentrations of cilnidipine used (20, 40, and 60 µΜ). The inhibitory effect of 40 µM cilnidipine on detrusor contractility was significantly reversed by the addition of FPL64716 and GV-58.
CONCLUSIONS
Cilnidipine inhibits the contractility of the isolated detrusor by blocking L- and N-type calcium channels. Cilnidipine could be evaluated for treating clinical conditions requiring relaxation of the detrusor such as overactive bladder.
PubMed: 37869401
DOI: 10.1016/j.curtheres.2023.100717 -
ACS Omega Jun 2020To recover potassium from feldspar, a biowaste, i.e., eggshell, was used. The chief composition of eggshells is calcite. As it is a rich source of Ca, hence it is used...
To recover potassium from feldspar, a biowaste, i.e., eggshell, was used. The chief composition of eggshells is calcite. As it is a rich source of Ca, hence it is used with HCl to produce calcium chloride. Feldspar is an aluminosilicate mineral that bears potassium in the interstitial sites. To unlock the potassium from the interstitial sites, it was roasted with calcium chloride prepared by mixing eggshell and hydrochloric acid. At the roasting temperature, CaCl melts and penetrates into the aluminosilicate matrix to replace K with Ca. Potassium ion released from the silicate matrix combines with chloride ions to form potassium chloride, which solubilized in water during the leaching process of the roasted feldspar. For elucidation of the mechanism of the roasting process, the shrinking core model was applied to the roast-leach data, and diffusion through the product layer was inferred as the rate-determining step. The order of the roasting process was found to be 2.158 and activation energy calculated to be 155.3 kJ/mol. Apart from potassium, sodium and excess calcium also got co-leached. To recover potassium from the leach liquor selectively, sodium perchlorate was added to precipitate potassium as KClO. Further, potassium perchlorate was thermally decomposed to give fertilizer grade potassium chloride (purity: 99.81%).
PubMed: 32637772
DOI: 10.1021/acsomega.0c00586