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Chemistry (Weinheim An Der Bergstrasse,... May 2020A general and efficient strategy for synthesis of tri-, hexa- and heptasaccharidic substructures of the lipopolysaccharide of Providencia rustigianii O34 is described....
A general and efficient strategy for synthesis of tri-, hexa- and heptasaccharidic substructures of the lipopolysaccharide of Providencia rustigianii O34 is described. For the heptasaccharide seven different building blocks were employed. Special features of the structures are an α-linked galactosamine and the two embedded α-fucose units, which are either branched at positions-3 and -4 or further linked at their 2-position. Convergent strategies focused on [4+3], [3+4], and [4+2+1] couplings. Whereas the [4+3] and [3+4] coupling strategies failed the [4+2+1] strategy was successful. As monosaccharidic building blocks trichloroacetimidates and phosphates were employed. Global deprotection of the fully protected structures was achieved by Birch reaction.
PubMed: 32092205
DOI: 10.1002/chem.202000496 -
Infection and Immunity Jun 2023Providencia rustigianii is potentially enteropathogenic in humans. Recently, we identified a strain carrying a part of the gene homologous to that of that produces an...
Providencia rustigianii is potentially enteropathogenic in humans. Recently, we identified a strain carrying a part of the gene homologous to that of that produces an exotoxin called cytolethal distending toxin (CDT), encoded by three subunit genes (, , and ). In this study, we analyzed the strain for possible presence of the entire gene cluster and its organization, location, and mobility, as well as expression of the toxin as a putative virulence factor of . Nucleotide sequence analysis revealed the presence of the three subunit genes in tandem, and over 94% homology to the corresponding genes carried by P. alcalifaciens both at nucleotide and amino acid sequence levels. The strain produced biologically active CDT, which caused distension of eukaryotic cell lines with characteristic tropism of CHO and Caco-2 cells but not of Vero cells. S1-nuclease digested pulsed-field gel electrophoresis followed by Southern hybridization analysis demonstrated that the genes in both and P. alcalifaciens strains are located on large plasmids (140 to 170 kb). Subsequently, conjugation assays using a genetically marked derivative of the strain showed that the plasmid carrying genes in the was transferable to gene-negative recipient strains of , Providencia rettgeri, and Escherichia coli. Our results demonstrated the presence of genes in for the first time, and further showed that the genes are located on a transferable plasmid, which can potentially spread to other bacterial species.
Topics: Animals; Chlorocebus aethiops; Humans; Providencia; Vero Cells; Caco-2 Cells; Escherichia coli
PubMed: 37158737
DOI: 10.1128/iai.00121-22 -
Plants (Basel, Switzerland) May 2023In this study, the biochemical, antioxidant properties, and antimicrobial activity of the epiphytic leafy liverwort (L.) Dumort were investigated. Due to the scarcity...
In this study, the biochemical, antioxidant properties, and antimicrobial activity of the epiphytic leafy liverwort (L.) Dumort were investigated. Due to the scarcity and difficulty in obtaining liverworts, research on their bioactivity is limited; thus, this study aimed to uncover the potential of . The antimicrobial activity was evaluated against various microorganisms, including food isolates, clinical isolates, multidrug-resistant strains, and standard strains, using the disk diffusion method and determining the minimum inhibitory concentration (MIC) values. This study represents the first antioxidant investigation on and an antimicrobial study using ethanol extract and the disk diffusion method. Notably, susceptibility was observed in ATCC 29212, FI, ATCC 7644, MDR, and ATCC 25923. The antioxidant capacity was assessed using the DPPH method, emphasizing the high scavenging performance. Gas chromatography-mass spectrometry (GC-MS) analysis identified the primary compounds as frullanolide (19.08%), 2,3-Dimethylanisole (15.21%), linoleic acid (11.11%), palmitic acid (9.83%), and valerenic acid (5.3%). The results demonstrated the significant antimicrobial activity of against the tested microorganisms and its potent antioxidant properties. These findings emphasize the potential of as a promising source of natural antimicrobial and antioxidant agents, underscoring the importance of further investigation into its bioactive compounds and elucidating the mechanisms of action in future studies.
PubMed: 37176934
DOI: 10.3390/plants12091877