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Molecules (Basel, Switzerland) Jun 2020Near-infrared (NIR) spectroscopy occupies a specific spot across the field of bioscience and related disciplines. Its characteristics and application potential differs... (Review)
Review
Near-infrared (NIR) spectroscopy occupies a specific spot across the field of bioscience and related disciplines. Its characteristics and application potential differs from infrared (IR) or Raman spectroscopy. This vibrational spectroscopy technique elucidates molecular information from the examined sample by measuring absorption bands resulting from overtones and combination excitations. Recent decades brought significant progress in the instrumentation (e.g., miniaturized spectrometers) and spectral analysis methods (e.g., spectral image processing and analysis, quantum chemical calculation of NIR spectra), which made notable impact on its applicability. This review aims to present NIR spectroscopy as a matured technique, yet with great potential for further advances in several directions throughout broadly understood bio-applications. Its practical value is critically assessed and compared with competing techniques. Attention is given to link the bio-application potential of NIR spectroscopy with its fundamental characteristics and principal features of NIR spectra.
Topics: Animals; Blood Chemical Analysis; Blood Glucose; Humans; Oxygen; Spectroscopy, Near-Infrared; Spectrum Analysis, Raman
PubMed: 32604876
DOI: 10.3390/molecules25122948 -
Journal of the American Chemical Society Nov 2022In chemical biology research, various fluorescent probes have been developed and used to visualize target proteins or molecules in living cells and tissues, yet there... (Review)
Review
In chemical biology research, various fluorescent probes have been developed and used to visualize target proteins or molecules in living cells and tissues, yet there are limitations to this technology, such as the limited number of colors that can be detected simultaneously. Recently, Raman spectroscopy has been applied in chemical biology to overcome such limitations. Raman spectroscopy detects the molecular vibrations reflecting the structures and chemical conditions of molecules in a sample and was originally used to directly visualize the chemical responses of endogenous molecules. However, our initial research to develop "Raman tags" opens a new avenue for the application of Raman spectroscopy in chemical biology. In this Perspective, we first introduce the label-free Raman imaging of biomolecules, illustrating the biological applications of Raman spectroscopy. Next, we highlight the application of Raman imaging of small molecules using Raman tags for chemical biology research. Finally, we discuss the development and potential of Raman probes, which represent the next-generation probes in chemical biology.
Topics: Spectrum Analysis, Raman; Vibration; Proteins; Fluorescent Dyes; Biology
PubMed: 36216344
DOI: 10.1021/jacs.2c05359 -
Applied Spectroscopy Apr 2022This Focal Point Review paper discusses the developments of biomedical Raman and infrared spectroscopy, and the recent strive towards these technologies being regarded... (Review)
Review
This Focal Point Review paper discusses the developments of biomedical Raman and infrared spectroscopy, and the recent strive towards these technologies being regarded as reliable clinical tools. The promise of vibrational spectroscopy in the field of biomedical science, alongside the development of computational methods for spectral analysis, has driven a plethora of proof-of-concept studies which convey the potential of various spectroscopic approaches. Here we report a brief review of the literature published over the past few decades, with a focus on the current technical, clinical, and economic barriers to translation, namely the limitations of many of the early studies, and the lack of understanding of clinical pathways, health technology assessments, regulatory approval, clinical feasibility, and funding applications. The field of biomedical vibrational spectroscopy must acknowledge and overcome these hurdles in order to achieve clinical efficacy. Current prospects have been overviewed with comment on the advised future direction of spectroscopic technologies, with the aspiration that many of these innovative approaches can ultimately reach the frontier of medical diagnostics and many clinical applications.
Topics: Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman; Vibration
PubMed: 34041957
DOI: 10.1177/00037028211021846 -
FEBS Letters Oct 2022
Topics: Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman
PubMed: 36215224
DOI: 10.1002/1873-3468.14498 -
Journal of the American Chemical Society Sep 2019Carbohydrates possess a variety of distinct features with stereochemistry playing a particularly important role in distinguishing their structure and function.... (Review)
Review
Carbohydrates possess a variety of distinct features with stereochemistry playing a particularly important role in distinguishing their structure and function. Monosaccharide building blocks are defined by a high density of chiral centers. Additionally, the anomericity and regiochemistry of the glycosidic linkages carry important biological information. Any carbohydrate-sequencing method needs to be precise in determining all aspects of this stereodiversity. Recently, several advances have been made in developing fast and precise analytical techniques that have the potential to address the stereochemical complexity of carbohydrates. This perspective seeks to provide an overview of some of these emerging techniques, focusing on those that are based on NMR and MS-hybridized technologies including ion mobility spectrometry and IR spectroscopy.
Topics: Carbohydrate Sequence; Carbohydrates; Spectrum Analysis; Structure-Activity Relationship
PubMed: 31403778
DOI: 10.1021/jacs.9b06406 -
PloS One 2023We report a waveguide-enhanced Raman spectroscopy (WERS) platform with alignment-tolerant under-chip grating input coupling. The demonstration is based on a 100-nm thick...
We report a waveguide-enhanced Raman spectroscopy (WERS) platform with alignment-tolerant under-chip grating input coupling. The demonstration is based on a 100-nm thick planar (slab) tantalum pentoxide (Ta2O5) waveguide and the use of benzyl alcohol (BnOH) and its deuterated form (d7- BnOH) as reference analytes. The use of grating couplers simplifies the WERS system by providing improved translational alignment tolerance, important for disposable chips, as well as contributing to improved Raman conversion efficiency. The use of non-volatile, non-toxic BnOH and d7-BnOH as chemical analytes results in easily observable shifts in the Raman vibration lines between the two forms, making them good candidates for calibrating Raman systems. The design and fabrication of the waveguide and grating couplers are described, and a discussion of further potential improvements in performance is presented.
Topics: Benzyl Alcohol; Spectrum Analysis, Raman
PubMed: 37561713
DOI: 10.1371/journal.pone.0284058 -
Cytometry. Part a : the Journal of the... Aug 2019
Topics: Color; Flow Cytometry; Humans; Optics and Photonics; Spectrum Analysis
PubMed: 31038271
DOI: 10.1002/cyto.a.23779 -
International Journal of Molecular... Dec 2022Surface-enhanced Raman spectroscopy (SERS) is an ultra-sensitive and rapid technique that is able to significantly enhance the Raman signals of analytes absorbed on...
Surface-enhanced Raman spectroscopy (SERS) is an ultra-sensitive and rapid technique that is able to significantly enhance the Raman signals of analytes absorbed on functional substrates by orders of magnitude. Recently, semiconductor-based SERS substrates have shown rapid progress due to their great cost-effectiveness, stability and biocompatibility. In this work, three types of faceted CoO microcrystals with dominantly exposed {100} facets, {111} facets and co-exposed {100}-{111} facets (denoted as C-100, C-111 and C-both, respectively) are utilized as SERS substrates to detect the rhodamine 6G (R6G) molecule and nucleic acids (adenine and cytosine). C-100 exhibited the highest SERS sensitivity among these samples, and the lowest detection limits (LODs) to R6G and adenine can reach 10 M. First-principles density functional theory (DFT) simulations further unveiled a stronger photoinduced charge transfer (PICT) in C-100 than in C-111. This work provides new insights into the facet-dependent SERS for semiconductor materials.
Topics: Metal Nanoparticles; Spectrum Analysis, Raman; Semiconductors
PubMed: 36555570
DOI: 10.3390/ijms232415930 -
Nano Letters Jul 2022Picocavities are sub-nanometer-scale optical cavities recently found to trap light, which are formed by single-atom defects on metallic facets. Here, we develop simple...
Picocavities are sub-nanometer-scale optical cavities recently found to trap light, which are formed by single-atom defects on metallic facets. Here, we develop simple picocavity models and discuss what is known and unknown about this new domain of atom-scale optics, as well as the challenges for developing comprehensive theories. We provide simple analytic expressions for many of their key properties and discuss a range of applications from molecular electronics to photocatalysis where picocavities are important.
Topics: Optics and Photonics; Spectrum Analysis, Raman
PubMed: 35793541
DOI: 10.1021/acs.nanolett.2c01695 -
Journal of Visualized Experiments : JoVE 2023
Topics: Microscopy; Spectrum Analysis, Raman
PubMed: 37274091
DOI: 10.3791/64882