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FEBS Letters Oct 2022
Topics: Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman
PubMed: 36215224
DOI: 10.1002/1873-3468.14498 -
Kidney International Sep 2017In this review, we will highlight technologies that enable scientists to study the molecular characteristics of tissues and/or cells without the need for antibodies or... (Review)
Review
In this review, we will highlight technologies that enable scientists to study the molecular characteristics of tissues and/or cells without the need for antibodies or other labeling techniques. Specifically, we will focus on matrix-assisted laser desorption/ionization imaging mass spectrometry, infrared spectroscopy, and Raman spectroscopy.
Topics: Humans; Kidney; Kidney Diseases; Molecular Imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Infrared; Spectrum Analysis, Raman; Vibration
PubMed: 28750926
DOI: 10.1016/j.kint.2017.03.052 -
Cellular and Molecular Life Sciences :... Oct 2008Metallomics and metalloproteomics are emerging fields addressing the role, uptake, transport and storage of trace metals essential for protein functions. The... (Review)
Review
Metallomics and metalloproteomics are emerging fields addressing the role, uptake, transport and storage of trace metals essential for protein functions. The methodologies utilized in metallomics and metalloproteomics to provide information on the identity, quantity and function of metalloproteins are discussed. The most widely used approach is through inductively coupled plasma mass spectrometry to identify the metal bound to a protein, and electrospray ionization mass spectrometry to elucidate the structure, dynamics and function of a metal-protein complex. Other approaches include X-ray absorption and X-ray fluorescence spectroscopies, and bioinformatics sequence analysis. X-ray absorption spectroscopy utilizing a synchrotron radiation source is a powerful tool to provide a direct analysis of metal bound to proteins and proteomic metal distribution in biological matrices. With the advent of genome sequencing, a large database of protein primary structures has been established, and specific tools to identify metalloproteins in the genome sequences have been developed.
Topics: Computational Biology; Mass Spectrometry; Metalloproteins; Metals; Models, Molecular; Protein Conformation; Proteomics; Spectrum Analysis; Synchrotrons
PubMed: 18560755
DOI: 10.1007/s00018-008-8189-9 -
Scientific Reports Apr 2021Preliminary study has been made of black human hair, carbon concentration of some 53%, a model in examining the potential of hair of the human head in retrospective and...
Preliminary study has been made of black human hair, carbon concentration of some 53%, a model in examining the potential of hair of the human head in retrospective and emergency biodosimetry applications, also offering effective atomic number near to that of water. The hair samples were exposed to [Formula: see text]Co gamma rays, delivering doses from 0 to 200 Gy. Structural alterations were observed, use being made of Raman and photoluminescence (PL) spectroscopy. Most prominent among the features observed in the first-order Raman spectra are the D and G peaks, appearing at 1370 [Formula: see text] and 1589 [Formula: see text] respectively, the intensity ratio [Formula: see text] indicating dose-dependent defects generation and annealing of structural alterations. The wavelengths of the PL absorption and emission peaks are found to be centred at [Formula: see text] nm and [Formula: see text] nm, respectively. The hair samples mean band gap energy ([Formula: see text]) post-irradiation was found to be [Formula: see text] eV, of the order of a semiconductor and approximately two times the [Formula: see text] of other carbon-rich materials reported via the same methodology.
Topics: Elements; Female; Gamma Rays; Hair; Humans; Luminescence; Spectrometry, X-Ray Emission; Spectrum Analysis, Raman
PubMed: 33846448
DOI: 10.1038/s41598-021-86942-4 -
Biotechnology Advances 2014Advances in nanotechnology have opened up a new era of diagnosis, prevention and treatment of diseases and traumatic injuries. Nanomaterials, including those with... (Review)
Review
Advances in nanotechnology have opened up a new era of diagnosis, prevention and treatment of diseases and traumatic injuries. Nanomaterials, including those with potential for clinical applications, possess novel physicochemical properties that have an impact on their physiological interactions, from the molecular level to the systemic level. There is a lack of standardized methodologies or regulatory protocols for detection or characterization of nanomaterials. This review summarizes the techniques that are commonly used to study the size, shape, surface properties, composition, purity and stability of nanomaterials, along with their advantages and disadvantages. At present there are no FDA guidelines that have been developed specifically for nanomaterial based formulations for diagnostic or therapeutic use. There is an urgent need for standardized protocols and procedures for the characterization of nanoparticles, especially those that are intended for use as theranostics.
Topics: Chemistry, Physical; Circular Dichroism; Contrast Media; Humans; Light; Magnetic Resonance Spectroscopy; Mass Spectrometry; Microscopy, Atomic Force; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Microscopy, Scanning Tunneling; Molecular Imaging; Nanomedicine; Nanoparticles; Nanostructures; Nanotechnology; Scattering, Radiation; Spectrometry, Fluorescence; Spectrophotometry, Infrared; Spectrum Analysis, Raman; Surface Properties; Technology, Pharmaceutical
PubMed: 24252561
DOI: 10.1016/j.biotechadv.2013.11.006 -
Analytical Chemistry Jul 2022Glycan analysis has evolved considerably during the last decade. The advent of high-resolution ion-mobility spectrometry has enabled the separation of isomers with only...
Glycan analysis has evolved considerably during the last decade. The advent of high-resolution ion-mobility spectrometry has enabled the separation of isomers with only the slightest of structural differences. However, the ability to separate such species raises the problem of identifying all the mobility-resolved peaks that are observed, especially when analytical standards are not available. In this work, we report an approach based on the combination of IMS with cryogenic vibrational spectroscopy to identify -glycan reducing-end anomers. By identifying the reducing-end α and β anomers of diacetyl-chitobiose, which is a disaccharide that forms part of the common core of all -glycans, we are able to assign mobility peaks to reducing anomers of a selection of -glycans of different sizes, starting from trisaccharides such as Man-1 up to glycans containing nine monosaccharide units, such as G2. By building an infrared fingerprint database of the identified -glycans, our approach allows unambiguous identification of mobility peaks corresponding to reducing-end anomers and distinguishes them from positional isomers that might be present in a complex mixture.
Topics: Humans; Ion Mobility Spectrometry; Isomerism; Polysaccharides; Spectrum Analysis
PubMed: 35797429
DOI: 10.1021/acs.analchem.2c01181 -
International Journal of Molecular... Apr 2023The review briefly describes various types of infrared (IR) and Raman spectroscopy methods. At the beginning of the review, the basic concepts of biological methods of... (Review)
Review
The review briefly describes various types of infrared (IR) and Raman spectroscopy methods. At the beginning of the review, the basic concepts of biological methods of environmental monitoring, namely bioanalytical and biomonitoring methods, are briefly considered. The main part of the review describes the basic principles and concepts of vibration spectroscopy and microspectrophotometry, in particular IR spectroscopy, mid- and near-IR spectroscopy, IR microspectroscopy, Raman spectroscopy, resonance Raman spectroscopy, Surface-enhanced Raman spectroscopy, and Raman microscopy. Examples of the use of various methods of vibration spectroscopy for the study of biological samples, especially in the context of environmental monitoring, are given. Based on the described results, the authors conclude that the near-IR spectroscopy-based methods are the most convenient for environmental studies, and the relevance of the use of IR and Raman spectroscopy in environmental monitoring will increase with time.
Topics: Vibration; Biological Monitoring; Spectrophotometry, Infrared; Spectrum Analysis, Raman; Spectroscopy, Near-Infrared; Spectroscopy, Fourier Transform Infrared
PubMed: 37108111
DOI: 10.3390/ijms24086947 -
Journal of the American Society For... Dec 2020Imaging mass spectrometry has become a mature molecular mapping technology that is used for molecular discovery in many medical and biological systems. While powerful by... (Review)
Review
Imaging mass spectrometry has become a mature molecular mapping technology that is used for molecular discovery in many medical and biological systems. While powerful by itself, imaging mass spectrometry can be complemented by the addition of other orthogonal, chemically informative imaging technologies to maximize the information gained from a single experiment and enable deeper understanding of biological processes. Within this review, we describe MALDI, SIMS, and DESI imaging mass spectrometric technologies and how these have been integrated with other analytical modalities such as microscopy, transcriptomics, spectroscopy, and electrochemistry in a field termed multimodal imaging. We explore the future of this field and discuss forthcoming developments that will bring new insights to help unravel the molecular complexities of biological systems, from single cells to functional tissue structures and organs.
Topics: Animals; Electrochemical Techniques; Gene Expression Profiling; Humans; Mass Spectrometry; Microscopy; Multimodal Imaging; Spectrophotometry, Infrared; Spectrum Analysis, Raman; Transcriptome
PubMed: 32886506
DOI: 10.1021/jasms.0c00232 -
Molecules (Basel, Switzerland) Oct 2020Vibrational spectroscopy (mid-infrared (IR) and Raman) and its fingerprinting capabilities offer rapid, high-throughput, and non-destructive analysis of a wide range of... (Review)
Review
Vibrational spectroscopy (mid-infrared (IR) and Raman) and its fingerprinting capabilities offer rapid, high-throughput, and non-destructive analysis of a wide range of sample types producing a characteristic chemical "fingerprint" with a unique signature profile. Nuclear magnetic resonance (NMR) spectroscopy and an array of mass spectrometry (MS) techniques provide selectivity and specificity for screening metabolites, but demand costly instrumentation, complex sample pretreatment, are labor-intensive, require well-trained technicians to operate the instrumentation, and are less amenable for implementation in clinics. The potential for vibration spectroscopy techniques to be brought to the bedside gives hope for huge cost savings and potential revolutionary advances in diagnostics in the clinic. We discuss the utilization of current vibrational spectroscopy methodologies on biologic samples as an avenue towards rapid cost saving diagnostics.
Topics: Magnetic Resonance Spectroscopy; Metabolome; Metabolomics; Spectrophotometry, Infrared; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman; Vibration
PubMed: 33076318
DOI: 10.3390/molecules25204725 -
Metallomics : Integrated Biometal... Jan 2019To know how much of a metal species is in a particular location within a biological context at any given time is essential for understanding the intricate roles of... (Review)
Review
To know how much of a metal species is in a particular location within a biological context at any given time is essential for understanding the intricate roles of metals in biology and is the fundamental question upon which the field of metallomics was born. Simply put, seeing is powerful. With the combination of spectroscopy and microscopy, we can now see metals within complex biological matrices complemented by information about associated molecules and their structures. With the addition of mass spectrometry and particle beam based techniques, the field of view grows to cover greater sensitivities and spatial resolutions, addressing structural, functional and quantitative metallomic questions from the atomic level to whole body processes. In this perspective, I present a paradigm shift in the way we relate to and integrate current and developing metallomic analytics, highlighting both familiar and perhaps less well-known state of the art techniques for in situ metallomic imaging, specific biological applications, and their use in correlative studies. There is a genuine need to abandon scientific silos and, through the establishment of a metallomic scientific platform for further development of multidimensional analytics for in situ metallomic imaging, we have an incredible opportunity to enhance the field of metallomics and demonstrate how discovery research can be done more effectively.
Topics: Animals; Computational Biology; Electron Spin Resonance Spectroscopy; Humans; Magnetic Resonance Imaging; Mass Spectrometry; Metalloproteins; Metals; Microscopy; Microscopy, Electron; Optical Imaging; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman
PubMed: 30499574
DOI: 10.1039/c8mt00235e