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The Medical Journal of Australia Aug 1987A patient from Vanuatu with a mycetoma of the foot of 25 years' duration is described. Culture of a biopsy specimen from the foot grew a fungus which was identified as...
A patient from Vanuatu with a mycetoma of the foot of 25 years' duration is described. Culture of a biopsy specimen from the foot grew a fungus which was identified as Acremonium falciforme. This agent has not been described previously in association with mycetomas in the Pacific region.
Topics: Acremonium; Foot Dermatoses; Humans; Male; Middle Aged; Mycetoma; Mycoses
PubMed: 3657631
DOI: 10.5694/j.1326-5377.1987.tb133355.x -
Natural Product Research Sep 2021The sterol 3β,5α,6β,7α-tetrahydroxyergosta-8(14),22-diene was obtained from bio-guided fractioning of the chloroform extract of 50 L of liquid culture of . This...
The sterol 3β,5α,6β,7α-tetrahydroxyergosta-8(14),22-diene was obtained from bio-guided fractioning of the chloroform extract of 50 L of liquid culture of . This fungal strain was selected because of its anti-proliferative activity against solid human tumour cell lines (GI ≤ 50 μg/mL) in a bio-prospective study of fungi isolated from plant material, sediment and water samples obtained from alkaline lakes Alchichica and Atexcac in Puebla, Mexico. This compound showed GI (μM) values of: 16, 24, 18, 15 and 12 against tumour cell lines A-549, HBL-100, HeLa, T-47D and WiDr respectively. GI effects against tumour lines T-47D and WiDr were found to be greater than the clinically used drugs Etoposide and Cisplatin. Because of this, the results obtained support the pharmacological importance of the microorganisms that develop in these ecosystems and strengthen the non-invasive bio-prospection studies that our work group has developed in recent years.
Topics: Acremonium; Antineoplastic Agents; Cell Line, Tumor; Humans; Lakes; Mexico
PubMed: 31556322
DOI: 10.1080/14786419.2019.1669032 -
Microbial Cell Factories Jun 2018Cephalosporin C (CPC) produced by Acremonium chrysogenum is one of the most important drugs for treatment of bacterial infectious diseases. As the major stimulant,...
BACKGROUND
Cephalosporin C (CPC) produced by Acremonium chrysogenum is one of the most important drugs for treatment of bacterial infectious diseases. As the major stimulant, methionine is widely used in the industrial production of CPC. In this study, we found methionine stimulated CPC production through enhancing the accumulation of endogenous S-adenosylmethionine (SAM). To overcome the methionine dependent stimulation of CPC production, the methionine cycle of A. chrysogenum was reconstructed by metabolic engineering.
RESULTS
Three engineered strains were obtained by overexpressing the SAM synthetase gene AcsamS and the cystathionine-γ-lyase gene mecB, and disrupting a SAM dependent methyltransferase gene Acppm1, respectively. Overexpression of AcsamS resulted in fourfold increase of CPC production which reached to 129.7 µg/mL. Disruption of Acppm1 also increased CPC production (up to 135.5 µg/mL) through enhancing the accumulation of intracellular SAM. Finally, an optimum recombinant strain (Acppm1DM-mecBOE) was constructed through overexpressing mecB in the Acppm1 disruption mutant. In this strain, CPC production reached to the maximum value (142.7 µg/mL) which was 5.5-fold of the wild-type level and its improvement was totally independent of methionine stimulation.
CONCLUSIONS
In this study, we constructed a recombinant strain in which the improvement of CPC production was totally independent of methionine stimulation. This work provides an economic route for improving CPC production in A. chrysogenum through metabolic engineering.
Topics: Acremonium; Cephalosporins; Metabolic Engineering; Methionine
PubMed: 29879990
DOI: 10.1186/s12934-018-0936-5 -
International Journal of Dermatology Jul 2001
Topics: Acremonium; Adult; Dermatomycoses; Facial Dermatoses; Female; Humans; Immunocompetence
PubMed: 11679001
DOI: 10.1046/j.1365-4362.2001.01220.x -
Advances in Biochemical... 2000Process monitoring of cephalosporin C formation by Acremonium chrysogenum in laboratory investigations is considered. The goal of these investigations is the... (Review)
Review
Process monitoring of cephalosporin C formation by Acremonium chrysogenum in laboratory investigations is considered. The goal of these investigations is the identification of bottlenecks in the biosynthesis and the improvement of the process performance. Based on reports of other research groups and own experience the key parameters were selected, which influence the process performance. They are: dissolved oxygen and pH values. In addition the concentrations of biomass, DNA, glucose and reducing sugars (glucose, maltose, maltotriose and oligosaccharides), methionine, other nitrogen sources (ammonium ion, other amino acids), organic acids, phosphate, sulfate, dissolved organic carbon, proteins, product and precursors in the cell free cultivation medium are monitored. In addition the intracellular concentrations of RNA, DNA, proteins, amino acids as well as the activities of the enzymes of the biosynthesis of cephalosporin C are determined. The influence of these parameters on the biosynthesis is discussed.
Topics: Acremonium; Biomass; Biotechnology; Cephalosporins; Chromatography, High Pressure Liquid; Hydrogen-Ion Concentration; Models, Chemical; Oxygen; RNA, Fungal; Spectrometry, Fluorescence; Time Factors
PubMed: 10592528
DOI: 10.1007/3-540-48773-5_4 -
Natural Product Research Apr 2004Two related butenolides have been isolated from a culture broth of a strain of the fungus, Acremonium sp. Although the lactone acid (3) is a known compound, the...
Two related butenolides have been isolated from a culture broth of a strain of the fungus, Acremonium sp. Although the lactone acid (3) is a known compound, the structure of the lactone diacid (4), deduced by spectroscopic analysis, is assigned for the first time. This compound appears to be identical to a partly characterised metabolite isolated, along with 3, from Chaetomium indicum in 1953.
Topics: 4-Butyrolactone; Acremonium; Furans; Immunosuppressive Agents; Lactones; Spectrum Analysis
PubMed: 14984081
DOI: 10.1080/1478641032000101714 -
Fungal Genetics and Biology : FG & B Sep 2018Acremonium chrysogenum is the industrial producer of cephalosporin C (CPC). We isolated a mutant (AC554) from a T-DNA inserted mutant library of A. chrysogenum. AC554...
Acremonium chrysogenum is the industrial producer of cephalosporin C (CPC). We isolated a mutant (AC554) from a T-DNA inserted mutant library of A. chrysogenum. AC554 exhibited a reduced conidiation and lack of CPC production. In consistent with it, the transcription of cephalosporin biosynthetic genes pcbC and cefEF was significantly decreased in AC554. Thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) was performed and sequence analysis indicated that a T-DNA was inserted upstream of an open reading frame (ORF) which was designated AcmybA. On the basis of sequence analysis, AcmybA encodes a Myb domain containing transcriptional factor. Observation of red fluorescent protein (RFP) tagged AcMybA showed that AcMybA is naturally located in the nucleus of A. chrysogenum. Transcriptional analysis demonstrated that the AcmybA transcription was increased in AC554. In contrast, the AcmybA deleted mutant (ΔAcmybA) overproduced conidia and CPC. To screen the targets of AcmybA, we sequenced and compared the transcriptome of ΔAcmybA, AC554 and the wild-type strain at different developmental stages. Twelve differentially expressed regulatory genes were identified. Taken together, our results indicate that AcMybA negatively regulates conidiation and CPC production in A. chrysogenum.
Topics: Acremonium; Cephalosporins; Fungal Proteins; Gene Expression Regulation, Fungal; Luminescent Proteins; Spores, Fungal; Transcription Factors; Transcriptome; Red Fluorescent Protein
PubMed: 29870835
DOI: 10.1016/j.fgb.2018.05.006 -
Applied Biochemistry and Biotechnology Aug 2019Acremonium chrysogenum is an important fungal strain used for cephalosporin C production. Many efforts have been made to develop versatile genome-editing tools to better...
Acremonium chrysogenum is an important fungal strain used for cephalosporin C production. Many efforts have been made to develop versatile genome-editing tools to better understand the mechanism of A. chrysogenum. Here, we developed a feasible and efficient CRISPR/Cas9 system. Two genes responsible for the synthesis of yellow pigments (sorbicillinoids) were chosen as targets, and plasmids expressing both the Cas9 protein and single-guide RNAs were constructed. After introducing the plasmids into the protoplasts of A. chrysogenum, 83 to 93% albino mutants harboring the expected genomic alteration, on average, were obtained. We have generated two mutant strains that respectively disrupt sorA and sorB by flexible CRISPR/Cas9 system. We further confirmed that the sorbicillinoid biosynthetic gene cluster is regulated by an autoinduction mechanism. This work will lay a solid foundation for gene function research and regulation in the sorbicillinoid biosynthetic pathway.
Topics: Acremonium; CRISPR-Cas Systems; Gene Editing; Polyketide Synthases; RNA, Guide, CRISPR-Cas Systems
PubMed: 30809786
DOI: 10.1007/s12010-019-02960-z -
Journal of Natural Products Sep 2019Four new hydroxamate-containing natural product cyclopeptides designated acremonpeptides A-D (-), together with Al(III)-acremonpeptide D () were obtained from the marine...
Four new hydroxamate-containing natural product cyclopeptides designated acremonpeptides A-D (-), together with Al(III)-acremonpeptide D () were obtained from the marine fungus SCSIO 115. The planar structures of - were established on the basis of HRMS as well as 1D and 2D NMR data sets. Moreover, the amino acid absolute configurations were determined using Marfey's method. Compounds - all feature three 2-amino-5-(-hydroxyacetamido)pentanoic acid (-hydroxy--acetyl-l-ornithine) metal ion chelating moieties. Beyond their discovery and structure elucidation, bioassays revealed acremonpeptides A (), B (), and Al(III)-acremonpeptide D () as moderate antiviral agents for herpes simplex virus 1 with EC values of 16, 8.7, and 14 μM, respectively.
Topics: Acremonium; Aluminum; Marine Biology; Siderophores
PubMed: 31503476
DOI: 10.1021/acs.jnatprod.9b00545 -
Journal of Biochemistry May 1994The ascorbate oxidase obtained from a microorganism, Acremonium sp. HI-25 (molecular weight, 80 kDa; monomeric protein), was studied with respect to atomic absorption,... (Comparative Study)
Comparative Study
The ascorbate oxidase obtained from a microorganism, Acremonium sp. HI-25 (molecular weight, 80 kDa; monomeric protein), was studied with respect to atomic absorption, EPR, absorption spectra, circular dichroism (CD) spectra, and steady-state kinetics. The enzyme was found to be a multicopper protein, containing four copper atoms of three kinds, types 1, 2, and 3 copper, in the ratio of 1:1:2. The EPR parameters of the type 1 and 2 copper atoms in the ascorbate oxidase are very similar to those in the case of the ascorbate oxidase obtained from cucumber, which is a dimeric protein. The apparent Km and kcat values for ascorbic acid of the ascorbate oxidase from Acremonium sp. HI-25 are almost the same as those of the monomeric unit of the ascorbate oxidase from cucumber.
Topics: Acremonium; Ascorbate Oxidase; Copper; Cucumis sativus
PubMed: 7961590
DOI: 10.1093/oxfordjournals.jbchem.a124420