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The Journal of Antibiotics Jul 2018Two new compounds, designated as hamuramicins A (1) and B (2), were isolated from the cultured broth of an endophytic actinomycete Allostreptomyces sp. K12-0794 by...
Two new compounds, designated as hamuramicins A (1) and B (2), were isolated from the cultured broth of an endophytic actinomycete Allostreptomyces sp. K12-0794 by silica gel column chromatography and HPLC. The structures of 1 and 2 were elucidated as 22-membered macrolide containing triene and trienone with an alkyl side chain by spectroscopic analyses including NMR experiments. Both compounds showed growth inhibition activity against Kocuria rhizophia and Xanthomonas oryzae pv. oryzae as well as human cell line toxicity.
Topics: Actinomycetaceae; Anti-Bacterial Agents; Antibiotics, Antineoplastic; Bacteria; Cell Line, Tumor; Chromatography, High Pressure Liquid; Endophytes; Fermentation; Humans; Macrolides; Magnetic Resonance Spectroscopy; Microbial Sensitivity Tests
PubMed: 29691484
DOI: 10.1038/s41429-018-0055-x -
International Journal of Systematic and... Jul 2016Actinobaculum massiliense (Euzéby, 2006) was isolated from the urine of an elderly woman in 2001. Unfortunately, the strain deposited as the type strain was, by error,...
Actinobaculum massiliense (Euzéby, 2006) was isolated from the urine of an elderly woman in 2001. Unfortunately, the strain deposited as the type strain was, by error, an Actinobaculum schaalii strain (Yassin et al., 2015). In 2015, we isolated a new strain of A. massiliense, FC3, from the urine of a 12-year-old patient with acute cystitis. We herein present the characteristics of strain FC3 (=CSUR P1982=DSM 100580) and formally propose it as the neotype strain of A. massiliense.
Topics: Actinomycetaceae; Bacterial Typing Techniques; Base Composition; Child; Cystitis; DNA, Bacterial; Fatty Acids; Female; Humans; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 27117391
DOI: 10.1099/ijsem.0.001110 -
International Journal of Oral Science Mar 2013The objective of this study was to investigate the compositional profiles and microbial shifts of oral microbiota during head-and-neck radiotherapy. Bioinformatic...
The objective of this study was to investigate the compositional profiles and microbial shifts of oral microbiota during head-and-neck radiotherapy. Bioinformatic analysis based on 16S rRNA gene pyrosequencing was performed to assess the diversity and variation of oral microbiota of irradiated patients. Eight patients with head and neck cancers were involved in this study. For each patient, supragingival plaque samples were collected at seven time points before and during radiotherapy. A total of 147,232 qualified sequences were obtained through pyrosequencing and bioinformatic analysis, representing 3,460 species level operational taxonomic units (OTUs) and 140 genus level taxa. Temporal variations were observed across different time points and supported by cluster analysis based on weighted UniFrac metrics. Moreover, the low evenness of oral microbial communities in relative abundance was revealed by Lorenz curves. This study contributed to a better understanding of the detailed characterization of oral bacterial diversity of irradiated patients.
Topics: Actinomyces; Actinomycetaceae; Alcaligenaceae; Bacteria; Capnocytophaga; Carnobacteriaceae; Computational Biology; Dental Plaque; Follow-Up Studies; Gemella; Head and Neck Neoplasms; High-Throughput Nucleotide Sequencing; Humans; Middle Aged; Neisseria; Prevotella; Propionibacteriaceae; RNA, Bacterial; RNA, Ribosomal, 16S; Streptococcus; Veillonella
PubMed: 23538641
DOI: 10.1038/ijos.2013.15 -
Folia Microbiologica Jan 2018The present study was designed to characterize phenotypically and genotypically a Trueperella pyogenes strain isolated from a brain abscess of an adult roebuck...
The present study was designed to characterize phenotypically and genotypically a Trueperella pyogenes strain isolated from a brain abscess of an adult roebuck (Capreolus capreolus). The species identity could be confirmed by phenotypical investigations, by MALDI-TOF MS analysis, and by sequencing the 16S ribosomal RNA (rRNA) gene, the 16S-23S rRNA intergenic spacer region (ISR); by sequencing the target genes rpoB, gap, and tuf; and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. The T. pyogenes strain could additionally be characterized by PCR-mediated amplification of several known and putative virulence factor-encoding genes which revealed the presence of the genes plo encoding pyolysin and nanH and nanP encoding neuraminidases; the genes fimA, fimC, and fimE encoding the fimbrial subunits FimA, FimC, and FimE; and the gene cbpA encoding collagen-binding protein CbpA. The present data give a detailed characterization of a T. pyogenes strain isolated from a brain abscess of a roebuck. However, the route of infection of the roebuck remains unclear.
Topics: Actinomycetaceae; Actinomycetales Infections; Animals; Bacterial Proteins; Brain Abscess; Deer; Male; Phylogeny; Virulence Factors
PubMed: 28534230
DOI: 10.1007/s12223-017-0533-8 -
APMIS : Acta Pathologica,... Feb 2018We report a case of aortic native valve endocarditis due to Actinotignum schaalii in an 89-year-old man with prostatism history but no signs of urinary infection....
We report a case of aortic native valve endocarditis due to Actinotignum schaalii in an 89-year-old man with prostatism history but no signs of urinary infection. Actinotignum schaalii was isolated not only from positive blood culture but also from cardiac valve culture using mass spectrometry and 16S rDNA sequencing. Actinotignum schaalii is recognized as commensal of genitourinary tract, but it was underdiagnosed. The advances in bacterial identification such as MALDI-TOF MS probably explain the increasing described cases of infections due to A. schaalii these last years.
Topics: Actinomycetaceae; Aged, 80 and over; Aortic Valve; Endocarditis, Bacterial; Heart Valve Diseases; Humans; Male; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 29700913
DOI: 10.1111/apm.12803 -
Microbial Pathogenesis Mar 2022Trueperella pyogenes is an opportunistic bacterial pathogen causing several infectious diseases, including metritis, mastitis and abscesses in domestic animals such as...
Trueperella pyogenes is an opportunistic bacterial pathogen causing several infectious diseases, including metritis, mastitis and abscesses in domestic animals such as dairy cattle. Several virulence proteins are released by T. pyogenes strains contributing to the pathogenic and causing disease potential of this pathogen. So far, many aspects of T. pyogenes pathogenesis are unknown. In this study, expression levels of plo, fimA, nanH and cbpA genes encoding pyolysin, fimbriae, neuraminidase and collagen-binding protein, respectively in T. pyogenes isolated from totally 15 metritis, mastitis and cutaneous abscesses convenience samples in response to co-culture with other pathogens including E. coli, St. dysgalactiae, S. aureus, F. necrophorum and L. plantarum strains in mice study model have been investigated. We found that expression levels of plo, fimA, nanH and cbpA genes in T. pyogenes isolates in response to co-culture with F. necrophorum and E. coli were significantly increased; however, no significant changes was seen in the level of expression of these genes in the isolates in response to co-culture with St. dysgalactiae and S. aureus. Notably, expression of all virulence factor genes was suppressed in T. pyogenes in response to co-culture with L. plantarum. We observed that L. plantarum might be used to prevent infectious diseases caused by T. pyogenes.
Topics: Actinomycetaceae; Actinomycetales Infections; Animals; Cattle; Coinfection; Escherichia coli; Female; Mice; Staphylococcus aureus; Virulence Factors
PubMed: 35121072
DOI: 10.1016/j.micpath.2022.105435 -
Antimicrobial Agents and Chemotherapy Sep 1977A strain of Actinomyces odontolyticus, originally isolated from human dental plaque, produced a non-dialyzable, trypsin-sensitive substance that was bactericidal for...
A strain of Actinomyces odontolyticus, originally isolated from human dental plaque, produced a non-dialyzable, trypsin-sensitive substance that was bactericidal for certain strains of bifidobacteria at 42 degrees C but not at 37 degrees C. Detectable quantities of the bacteriocin were not produced in liquid media. Experimentally useful yields were obtained by extraction from pour plate cultures of producer cells. At 42 degrees C, exponential killing did not occur until indicator cells had doubled at least once. At 37 degrees C, the bacteriocin effected a transient bacteriostasis. Partially purified concentrates were obtained by diethylaminoethyl-cellulose chromatography, and such material was not inactivated by ribonuclease, deoxyribonuclease, or lipase. Pronase, trypsin, and exposure to 100 degrees C for 20 min completely abolished activity. Inhibitory activity was considerably reduced by exposure to a pH of either 3 or 11. Treatment of producer cells with curing agents did not induce a high frequency of non-bacteriocinogenic cells. The odontolyticin was adsorbed by susceptible, as well as resistant, bacteria.
Topics: Actinomyces; Actinomycetaceae; Bacteriocins; Dental Plaque; Humans; Temperature
PubMed: 907331
DOI: 10.1128/AAC.12.3.410 -
Environmental Science & Technology Jul 2001Uptake of plutonium and uranium mediated by the siderophore desferrioxamine-B (DFOB) has been studied for the common soil aerobe Microbacterium flavescens(JG-9). M....
Uptake of plutonium and uranium mediated by the siderophore desferrioxamine-B (DFOB) has been studied for the common soil aerobe Microbacterium flavescens(JG-9). M. flavescens does not bind or take up nitrilotriacetic acid (NTA) complexes of U(VI), Fe(III), or Pu(IV) or U(VI)-DFOB but does take up Fe(III)-DFOB and Pu(IV)-DFOB. Pu(IV)-DFOB and Fe(III)-DFOB accumulations are similar: only living and metabolically active bacteria take up these metal-siderophore complexes. The Fe(III)-DFOB and Pu(IV)-DFOB complexes mutually inhibit uptake of the other, indicating that they compete for shared binding sites or uptake proteins. However, Pu uptake is much slower than Fe uptake, and cumulative Pu uptake is less than Fe, 1.0 nmol of Fe vs 0.25 nmol of Pu per mg of dry weight bacteria. The Pu(IV)-DFOB interactions with M. flavescens suggest that Pu-siderophore complexes could generally be recognized by Fe-siderophore uptake systems of many bacteria, fungi, or plants, thereby affecting Pu environmental mobility and distribution. The results also suggest that the siderophore complexes of tetravalent metals can be recognized by Fe-siderophore uptake proteins.
Topics: Actinomycetaceae; Binding Sites; Plutonium; Proteins; Siderophores; Soil Microbiology; Soil Pollutants, Radioactive; Uranium
PubMed: 11478246
DOI: 10.1021/es010590g -
BMC Research Notes Aug 2021Trueperella pyogenes has been considered a major causative agent of metritis, abortion, and death in a broad range of domestic and wild animals, including cattle, swine,...
OBJECTIVE
Trueperella pyogenes has been considered a major causative agent of metritis, abortion, and death in a broad range of domestic and wild animals, including cattle, swine, sheep, goats, camels, buffalo, deer, antelopes, reptiles, and birds.
DATA DESCRIPTION
Here, we report the complete chromosome sequence of Trueperella pyogenes strain Arash114, isolated from the uterus of a water buffalo (Bubalus bubalis) died due to the infection caused by this pathogen. The genome assembly comprised 2,338,282 bp, with a 59.5% GC content. Annotation of the genome showed 46 tRNA genes, 6 rRNA, 1 CRISPR and 2059 coding sequences. Also, several genes coding for antimicrobial resistance such as tetW and virulence factor including plo, nanH, nanP, cbp and 4 fimbrial proteins were found. This study will advance our knowledge regarding the metabolism, virulence factors, antibiotic resistance and evolution of Arash114 strain and serve as an appropriate template for future researches.
Topics: Actinomycetaceae; Animals; Buffaloes; Female; Iran; Uterus; Virulence Factors; Whole Genome Sequencing
PubMed: 34425879
DOI: 10.1186/s13104-021-05734-1 -
BJU International Aug 2013WHAT'S KNOWN ON THE SUBJECT? AND WHAT DOES THE STUDY ADD?: Actinobaculum schaalii is considered to be a part of the normai flora in the genital and urinary tract area....
UNLABELLED
WHAT'S KNOWN ON THE SUBJECT? AND WHAT DOES THE STUDY ADD?: Actinobaculum schaalii is considered to be a part of the normai flora in the genital and urinary tract area. It has been associated to urinary tract infection (UTI), sepsis, osteomyelitis, endocarditis and Foumier's gangrene. So far it has mainly been isolated from urine, blood and pus, and predominantly in elderly patients. This study examined the habitat of A. schaalii by collecting samples from skin and urine in patients with kidney or ureter stones before and after treatment with Extracorporeal Shock Wave Lithotripsy (ESWL). Additionally faeces and vaginal swabs from routine specimen in patients not undergoing ESWL and without known urinary calculi were also analysed. The study does not find A. schaalii in faeces but shows it to be presents on skin and mucosa in the genital area. A. schaalii is also shown a possible pathogen in the stone-patient group undergoing ESWL.
OBJECTIVE
To study the habitat of Actinobaculum schaalii by examing groin swabs, faeces samples and vaginal swabs, and to determine whether it is a common uropathogen in patients with kidney or ureter stones.
PATIENTS AND METHODS
A quantitative real-time PCR assay was used to analyse all samples, which were collected between 2010 and 2011. A total of 38 patients (24 men and 14 women), with kidney or ureter stones and undergoing extracorporeal shock wave lithotripsy (ESWL), provided urine samples and had groin swabs taken. In addition, 30 faecal samples and 19 vaginal swabs that had been sent for routine microbiological examinations from patients outside the ESWL group were analysed. A chi-squared test was used to analyse the differences between patient groups, studying samples from urine, faeces samples, groin swabs and vaginal swabs.
RESULTS
Actinobaculum schaalii was found in the urine samples from 14 (37%) patients undergoing ESWL, and in both urine and groin swabs from seven (18%) patients. Actinobaculum schaalii was not found in faeces samples but it was found in six (32%) of the vaginal swabs, predominantly in patients >50 years (P = 0.06).
CONCLUSION
The study indicates that A. schaalii is a commensal found on skin, urine and vaginal mucosa in the human urogenital area and supports other investigations in its finding that the elderly are at greatest risk of being colonized with A. schaalii.
Topics: Actinomycetaceae; Adult; Aged; Aged, 80 and over; Female; Humans; Male; Middle Aged; Skin; Urine; Urogenital System
PubMed: 23350855
DOI: 10.1111/j.1464-410X.2012.11739.x