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Reproduction in Domestic Animals =... Dec 2021The aim of this study was to identify the impact of Trueperella pyogenes in cows with clinical endometritis (CE) on reproductive performance and milk production in...
The aim of this study was to identify the impact of Trueperella pyogenes in cows with clinical endometritis (CE) on reproductive performance and milk production in affected cows. In total, 230 lactating Holstein dairy cows from six commercial dairy herds were sampled once between 28 and 33 days post-partum. Cows included in the present study did not receive antibiotic or anti-inflammatory treatments prior to the experimental period. Clinical endometritis (CE) was characterized as cow with vaginal mucus score = 3 (>50% of purulent vaginal discharge) and >18% polymorphonuclear neutrophilic leukocyte (PMNL). The body condition scores (BCS) and milk production were evaluated at the time of enrolment. The identification of isolated bacteria was carried out through the analysis of MALDI-TOF MS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry). According to uterine health, three groups of dairy cows were formed: healthy control cows without T. pyogenes (n = 147), CE cows with T. pyogenes (n = 22) and CE cows without T. pyogenes (n = 61). CE cows with T. pyogenes had lower BCS, milk production and conception at first AI (p < .01) than CE cows without T. pyogenes and control cows. Furthermore, CE cows with T. pyogenes had higher (p < .01) service per pregnancy and had greater (p < .01) days to get pregnant than CE cows without T. pyogenes and control cows. This study demonstrates that CE cows with T. pyogenes had impaired reproductive performance and milk production when compared to cows without CE and CE cows without T. pyogenes. This information can contribute to a strategic treatment in cows affected by clinical endometritis, favouring the rational use of antibiotics on dairy farms.
Topics: Actinomycetaceae; Animals; Bacterial Infections; Cattle; Cattle Diseases; Endometritis; Female; Lactation; Milk; Pregnancy; Reproduction
PubMed: 34510600
DOI: 10.1111/rda.14017 -
European Journal of Paediatric Dentistry Sep 2016To compare the composition of the salivary microbiota in caries-affected vs. caries-free mutans streptococci (MS)- positive children with mixed dentition. (Comparative Study)
Comparative Study
AIM
To compare the composition of the salivary microbiota in caries-affected vs. caries-free mutans streptococci (MS)- positive children with mixed dentition.
MATERIALS AND METHODS
Twenty eight healthy, 11-12-year-old schoolchildren with high MS counts (>10⊃5 CFU/mL) were included in this study. The children were screened with the Dentocult SM Strip Mutans test (Orion Diagnostica, Espoo, Finland) and examined using the International Caries Detection and Assessment System (ICDAS). The microbial composition of the saliva was assessed using the Human Oral Microbe Identification Microarray (HOMIM). Microbial differences between caries-affected (n=18) and caries-free children (n=10) were compared by Mann-Whitney analysis.
RESULTS
The microbiota of the caries-affected vs. caries-free children was rather similar. Abiotrophia defectiva and Actinomyces meyeri/A. odontolyticus were significantly higher in caries-affected than in caries-free children (p=0.006, 0.046, respectively). Shuttleworthia satelles was significantly higher in caries-free compared to caries-affected children (p=0.031). A. defectiva and A. meyeri/A. odontolyticus correlated positively with caries severity measured by ICDAS Caries Index (p = 0.494, 0.454, 0.400 respectively) while S. satelles was negatively correlated with caries severity (p= -0.489).
CONCLUSIONS
Salivary A. defectiva and A. meyeri/A. odontolyticus and are associated with caries occurrence in MS-positive children with mixed dentition.
Topics: Abiotrophia; Actinomyces; Actinomycetaceae; Bacterial Load; Carnobacteriaceae; Child; DMF Index; Dental Caries; Dentition, Mixed; Gemella; Gram-Positive Bacteria; Humans; Saliva; Streptococcus; Streptococcus mutans
PubMed: 27759406
DOI: No ID Found -
Acta Veterinaria Hungarica Sep 2017Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes are three bacterial pathogens closely associated with the bovine respiratory disease complex...
Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes are three bacterial pathogens closely associated with the bovine respiratory disease complex (BRDC). In the current study, a multiplex PCR for the simultaneous detection of these three bacteria in cultures was established. After serial optimisation, the detection limit of the method for the genomic DNA of the three bacteria was 40 pg/μl. The method could detect the genomic DNA of these three bacteria but not the genomic DNA of seven other bacterial strains. Together with the bacterial enrichment technology, the multiplex PCR could be used for detecting the three bacteria in animal tissues. This method might be valuable for speeding up laboratory diagnosis and directing the treatment of BRDC to these three bacterial pathogens.
Topics: Actinomycetaceae; Mannheimia haemolytica; Multiplex Polymerase Chain Reaction; Pasteurella multocida; Sensitivity and Specificity
PubMed: 28956490
DOI: 10.1556/004.2017.032 -
Microbiology Spectrum Dec 2017There is currently only limited information on the antimicrobial susceptibility and resistance of spp., spp., and from animals. The comparability of the data is...
There is currently only limited information on the antimicrobial susceptibility and resistance of spp., spp., and from animals. The comparability of the data is hampered by the use of different antimicrobial susceptibility testing methods and interpretive criteria. To date, standard broth microdilution methods and clinical breakpoints that are approved by the Clinical and Laboratory Standards Institute and are applicable to spp., spp., and are available. The lack of species-specific clinical breakpoints for the different animal species reduces the explanatory power of the data. Among the isolates of the three genera, elevated MICs for different classes of antimicrobial agents (e.g., β-lactams, macrolides, lincosamides, tetracyclines, aminoglycosides, phenicols, sulfonamides/diaminopyrimidines, and fluoroquinolones) have been described. The most comprehensive data set is available for , which also includes information about genes and mutations involved in antimicrobial resistance. In isolates, the macrolide-lincosamide-streptogramin B resistance genes (B) and (X) were identified. Tetracycline resistance in was based on the resistance genes (W), (Z), and (33), whereas the aminoglycoside resistance genes , , , , , and have been described in . So far, only single genes conferring either phenicol resistance (), trimethoprim resistance (), or β-lactam resistance () are known to occur in isolates. Various 23S rRNA mutations, including A2058T, A2058G, and G2137C, were identified in macrolide/lincosamide-resistant .
Topics: Actinomycetaceae; Animal Diseases; Animals; Anti-Bacterial Agents; Arcanobacterium; Corynebacterium; Drug Resistance, Bacterial; Genes, MDR; Microbial Sensitivity Tests; Mutation; RNA, Ribosomal, 23S; Species Specificity
PubMed: 29219109
DOI: 10.1128/microbiolspec.ARBA-0021-2017 -
Infection and Immunity Aug 1980The interaction of Bacterionema matruchotii with strains of Streptococcus sanguis produces a structure which morphologically resembles a corncob. To determine the...
The interaction of Bacterionema matruchotii with strains of Streptococcus sanguis produces a structure which morphologically resembles a corncob. To determine the specific bacterial surface receptors involved in the interaction, we developed a quantitative assay. The assay consisted of mixing saline suspensions of [CH(3)-(3)H]thymidine-labeled streptococci and B. matruchotii, incubating at 37 degrees C for 2 h, and filtering the mixture through a 5-mum polycarbonate membrane filter. The free cocci and filaments passed through the filter, but the corncobs were retained. Estimates of the number of corncobs formed were obtained by quantitating the radioactivity retained on the membranes relative to that of controls of streptococci alone. Although saturation of the Bacterionema occurred at a ratio of streptococci to Bacterionema of 10:1 (Klett units), a 2:1 ratio was chosen because of the increased sensitivity of the assay at this ratio. The percentage of streptococci binding at this ratio was 18.6 +/- 8.1 (standard deviation). All five Bacterionema strains tested formed corncobs; in contrast, only three strains of S. sanguis were positive. These were serotype 1 strains which had localized surface "fuzz." Although scanning electron microscopic observations revealed an almost random distribution of cocci along the filament surface, transmission electron microscopy revealed that the streptococci were attached to the Bacterionema by the surface fuzz. No differences in corncob formation were observed in sodium phosphate buffer, pH 6 to 8, at phosphate concentrations ranging from 0.005 to 0.05 M. Concentrations of NaCl or KCl up to 0.25 M did not affect corncob formation, and low concentrations of CaCl(2) increased corncob formation slightly, whereas MgCl(2), ethylenediaminetetraacetic acid, and citrate buffers reduced the number of streptococci binding to the filaments. These results suggest that divalent cations may play a role in this process.
Topics: Actinomycetaceae; Cell Membrane; Humans; Microbiological Techniques; Microscopy, Electron, Scanning; Microscopy, Phase-Contrast; Mouth; Streptococcus sanguis
PubMed: 7011981
DOI: 10.1128/iai.29.2.663-670.1980 -
Archives of Microbiology Jul 2017Trueperella pyogenes is a prevalent opportunistic bacterium that normally causes diverse suppurative lesions, endometritis and pneumonia in various economically...
Trueperella pyogenes is a prevalent opportunistic bacterium that normally causes diverse suppurative lesions, endometritis and pneumonia in various economically important animals. Although the genomic information of this species has been announced, little is known about its functional profiles. In this study, by performing a comparative transcriptome analysis between the highly and moderately virulent T. pyogenes isolates, we found the expression of a LuxR-type DNA-binding response regulator, PloR, was significantly up-regulated in the highly virulent T. pyogenes. Protein crystal structure prediction and primary functional assessment suggested that, the quorum-sensing signal molecules of Gram-negative bacteria such as Pseudomonas aeruginosa and Escherichia coli could significantly inhibit the growth, biofilm production and hemolysis of T. pyogenes by binding to the upstream sensor histidine kinase, PloS. Therefore, the PloS/PlosR two-component regulatory system might dominate the virulence of T. pyogenes. Our findings provide a major advance in understanding the pathogenesis of T. pyogenes, and may shed new light on the development of novel therapeutic strategies to control T. pyogenes infection.
Topics: Actinomycetaceae; Actinomycetales Infections; Animals; Anti-Infective Agents; Biofilms; DNA-Binding Proteins; Escherichia coli; Female; Gene Expression Profiling; Histidine Kinase; Pseudomonas aeruginosa; Quorum Sensing; Virulence; Virulence Factors
PubMed: 28144921
DOI: 10.1007/s00203-017-1338-5 -
Journal of Clinical Microbiology Jul 1994Colony morphology, beta hemolysis on horse blood agar, beta-glucuronidase activity, and ability to ferment sucrose and/or trehalose defined two biotypes of...
Colony morphology, beta hemolysis on horse blood agar, beta-glucuronidase activity, and ability to ferment sucrose and/or trehalose defined two biotypes of Arcanobacterium haemolyticum. One, the smooth type, grew as smooth, beta-hemolytic colonies and was beta-glucuronidase negative but often fermented sucrose and/or trehalose, while the other, the rough type, grew as rough colonies and was nonhemolytic, beta-glucuronidase positive, and negative for sucrose and trehalose fermentation. About 75% of the A. haemolyticum strains studied (n = 138) were of the smooth type. The smooth type predominated in wound infections, while the rough type was isolated almost exclusively from respiratory tract specimens; thus, 84% of the smooth-type strains were derived from wounds and 91% of the rough-type strains were isolated from respiratory tracts.
Topics: Actinomycetaceae; Adult; Bacterial Typing Techniques; Humans; Male; Microscopy, Electron; Middle Aged; Respiratory Tract Infections; Species Specificity; Wounds and Injuries
PubMed: 7929753
DOI: 10.1128/jcm.32.7.1654-1657.1994 -
Journal of General Microbiology Jul 1976Rothia dentocariosa was seen as a typical prokaryotic cell, lacking nuclear envelope, mitochondria and a reticulum with ribosomes. The plasma membrane was located close...
Rothia dentocariosa was seen as a typical prokaryotic cell, lacking nuclear envelope, mitochondria and a reticulum with ribosomes. The plasma membrane was located close to and parallel to the wall. The outer limits of the wall were associated with what may be capsular or slime material. Chain-like filaments of thick walled coccoid cells underwent septation both transverse and parallel to the long axis of the chain. Side branching and terminal clavate forms were also present. These clavate forms may represent specialized cells during the life-cycle. Fragmentation of the chain resulted when the outer wall ruptured to release the coccoid bodies.
Topics: Actinomycetaceae; Cell Membrane; Cell Wall; Microscopy, Electron; Morphogenesis; Mouth
PubMed: 956779
DOI: 10.1099/00221287-95-1-45 -
Anaerobe Aug 2015We report two cases of bacteremia with Actinobaculum schaalii, a rarely reported, anaerobic, Gram-positive bacterium. The first case was a patient with renal cancer who...
We report two cases of bacteremia with Actinobaculum schaalii, a rarely reported, anaerobic, Gram-positive bacterium. The first case was a patient with renal cancer who developed pyelonephritis after cryoablation, and the second was a patient who developed sepsis after a urogenital procedure. Bacteremia resolved after administration of empiric antibiotic therapy.
Topics: Actinomycetaceae; Actinomycetales Infections; Anti-Bacterial Agents; Bacteremia; Bacteria, Anaerobic; Female; Humans; Male; Middle Aged; Treatment Outcome
PubMed: 25881498
DOI: 10.1016/j.anaerobe.2015.04.006 -
Voprosy Pitaniia 1982
Review
Topics: Actinomycetaceae; Adolescent; Adult; Aging; Animals; Child; Child, Preschool; Female; Free Radicals; Humans; Infant; Infant, Newborn; Intestinal Mucosa; Intestines; Male; Maternal-Fetal Exchange; Nutritional Physiological Phenomena; Nutritional Requirements; Pantetheine; Pantothenic Acid; Pregnancy; Stimulation, Chemical
PubMed: 7041419
DOI: No ID Found