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Microbial Pathogenesis Mar 2018Aeromonas are ubiquitous in aquatic habitats. However some species can cause infections in humans, but rarely meningitis. Here we describe the isolation and...
Aeromonas are ubiquitous in aquatic habitats. However some species can cause infections in humans, but rarely meningitis. Here we describe the isolation and characterization of an Aeromonas strain from cerebrospinal fluid of a meningitis patient. The isolate, identified as A. trota by biochemical and molecular methods, was susceptible to ampicillin but resistant to cephalothin and cefazolin. Genome sequencing revealed virulence factor genes such as type VI secretion system, aerolysin and lateral flagella. The isolate exhibited swarming motility, hemolytic activity and adhesion and cytotoxicity on HeLa cells. This is the first report of A. trota associated with meningitis and its virulence characteristics.
Topics: Aeromonas; Anti-Bacterial Agents; Bacterial Typing Techniques; Cerebrospinal Fluid; Drug Resistance, Bacterial; Genome, Bacterial; Gram-Negative Bacterial Infections; Humans; Meningitis, Bacterial; Microbial Sensitivity Tests; Sequence Analysis, DNA; Virulence Factors
PubMed: 29355700
DOI: 10.1016/j.micpath.2018.01.017 -
Mikrobiologicheskii Zhurnal 1992Bacteria of genus Aeromonas are constant components of microbiota of fresh reservoirs where they, together with other microorganisms, play the part of natural biofilter... (Review)
Review
Bacteria of genus Aeromonas are constant components of microbiota of fresh reservoirs where they, together with other microorganisms, play the part of natural biofilter and promote water self-purification. They are necessarily present in normal microflora of hydrobionts inhabiting fresh reservoirs. The greatest attention is paid by the researchers to Aeromonas and biotrophs in connection with epizootics in aquaculture which have become more frequent, in particular, under fish breeding. That is why the review is, to more extent, concerned in the works of this trend made by the foreign and home researchers for the last decade.
Topics: Aeromonas; Aeromonas hydrophila; Animals; Bacterial Vaccines; Fish Diseases; Fisheries; Fishes; Gram-Negative Bacterial Infections; Virulence; Water Microbiology
PubMed: 1406386
DOI: No ID Found -
FEMS Microbiology Letters Apr 2019A recently described typing system based on sequence variation in the virulence array protein (vapA) gene, encoding the A-layer surface protein array, allows unambiguous...
A recently described typing system based on sequence variation in the virulence array protein (vapA) gene, encoding the A-layer surface protein array, allows unambiguous subtyping of Aeromonas salmonicida. In the present study, we compile A-layer typing results from a total of 675 A. salmonicida isolates, recovered over a 59-year period from 50 different fish species in 26 countries. Nine novel A-layer types (15-23) are identified, several of which display a strong predilection towards certain fish hosts, including e.g. Cyprinidae and Pleuronectidae species. Moreover, we find indications that anthropogenic transport of live fish may have aided the near global dissemination of two cyprinid-associated A-layer types. Comparison of whole genome phylogeny and A-layer typing for a subset of strains further resulted in compatible tree topologies, indicating the utility of vapA as a phylogenetic as well as an epizootiological marker in A. salmonicida. A Microreact project (microreact.org/project/r1pcOAx9m) has been created, allowing public access to the vapA analyses and relevant metadata. In sum, the results generated provide valuable insights into the global population structure of A. salmonicida, particularly in relation to its piscine host spectrum and the geographic distribution of these hosts.
Topics: Aeromonas salmonicida; Animals; Bacterial Proteins; Bacterial Typing Techniques; Fish Diseases; Gram-Negative Bacterial Infections; Phylogeny; Phylogeography; Virulence; Virulence Factors
PubMed: 30977802
DOI: 10.1093/femsle/fnz074 -
Journal of Fish Diseases Mar 2023Aeromonas veronii is an important zoonotic and aquatic pathogen that causes a number of illnesses in both humans and animals. It is related to gastroenteritis, skin and...
Aeromonas veronii is an important zoonotic and aquatic pathogen that causes a number of illnesses in both humans and animals. It is related to gastroenteritis, skin and soft tissue infections and bacteremia in humans, as well as causing significant economic losses in aquaculture owing to fish sepsis. Here, we constructed the flagellar cap gene (fliD) mutant strain of A. veronii by suicide plasmid-mediated homologous recombination system and analysed its characteristics. It was found that the deletion of fliD had no effect on growth and biochemical properties and could be inherited stably. However, the motility of A. veronii ΔfliD was significantly reduced, the flagellum was defective and the biofilm formation was attenuated compared with that of A. veronii wild-type strain. In vivo experiments revealed that the colonization capacity of ΔfliD was significantly lower than that of the wild-type strain in the period of first 24 h, and the median lethal dose (LD ) was 56 times higher than that of the wild-type strain. The Cyprinus carpio infected with the wild-type strain indicated faster death speed and more severe clinical signs compared to ΔfliD strain. These results suggest that fliD is closely related to the virulence of A. veronii and plays an important role in pathogenicity, providing the foundation for pathogenic mechanism studies of A. veronii.
Topics: Humans; Animals; Aeromonas veronii; Virulence; Carps; Fish Diseases; Gram-Negative Bacterial Infections; Aeromonas
PubMed: 36515399
DOI: 10.1111/jfd.13739 -
International Journal of Systematic and... Jun 2017A Gram-stain-negative, rod-shaped, strictly aerobic, motile bacterial strain, designated YM319T, was isolated from a seamount near the Yap Trench in the tropical western...
A Gram-stain-negative, rod-shaped, strictly aerobic, motile bacterial strain, designated YM319T, was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain YM319T was related to the genus Oceanisphaera and had highest 16S rRNA gene sequence similarities with the type strains Oceanisphaera profunda SM1222T (97.4 %), Oceanisphaera sediminis TW92T (97.3 %) and Oceanisphaera ostreae T-w6T (97.1 %). The predominant cellular fatty acids were summed feature 3 (composed of iso-C15 : 0 2-OH and/or C16 : 1 ω7c), C16 : 0 and C18 : 1ω7c. Strain YM319T had Q-8 as the predominant ubiquinone. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and four unidentified lipids. The genomic DNA G+C content of strain YM319T was 54.8 mol%. On the basis of the evidence presented in this study, strain YM319T represents a novel species of the genus Oceanisphaera, for which we propose the name Oceanisphaera marina sp. nov. (type strain YM319T=KACC 18564T=CGMCC 1.15923T).
Topics: Aeromonadaceae; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Pacific Ocean; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Ubiquinone
PubMed: 28629507
DOI: 10.1099/ijsem.0.001906 -
Archives of Microbiology May 2017Virulent Aeromonas hydrophila (vAh) is one of the most important bacterial pathogens that causes persistent outbreaks of motile Aeromonas septicemia in warm-water...
Virulent Aeromonas hydrophila (vAh) is one of the most important bacterial pathogens that causes persistent outbreaks of motile Aeromonas septicemia in warm-water fishes. The survivability of this pathogen in aquatic environments is of great concern. The aim of this study was to determine the capability of the vAh strain ML10-51K to degrade and utilize chitin. Genome-wide analysis revealed that ML10-51K encodes a suite of proteins for chitin metabolism. Assays in vitro showed that four chitinases, one chitobiase and one chitin-binding protein were secreted extracellularly and participated in chitin degradation. ML10-51K was shown to be able to use not only N-acetylglucosamine and colloidal chitin but also chitin flakes as sole carbon sources for growth. This study indicates that ML10-51K is a highly chitinolytic bacterium and suggests that the capability of effective chitin utilization could enable the bacterium to attain high densities when abundant chitin is available in aquatic niches.
Topics: Acetylglucosamine; Aeromonas hydrophila; Animals; Chitin; Chitinases
PubMed: 28032191
DOI: 10.1007/s00203-016-1326-1 -
Veterinary Microbiology Jan 2012Recent studies have revealed that the use of bdellovibrios is an alternative to control bacteriosis. However, no bdellovibrios are available against Aeromonas hydrophila...
Recent studies have revealed that the use of bdellovibrios is an alternative to control bacteriosis. However, no bdellovibrios are available against Aeromonas hydrophila infections in sturgeons. In the present study, a potential Bdellovibrio strain F16 was isolated from sturgeon gut samples, using a sturgeon-pathogenic A. hydrophila as the prey bacterium. It was initially identified as a Bdellovibrio strain using morphological characteristics and specific PCR amplification, and confirmed to be Bdellovibrio sp. strain ETB (GenBank Accession No. DQ302728) and Bdellovibrio bacteriovorus strain SRA9 (GenBank Accession No. AF263833) by phylogenetic analysis. In addition, it was shown to be safe for mammalians and sturgeons, had a wide prey range, and exhibited significant bacteriolytic effects on the pathogenic A. hydrophila. To the best of our knowledge, this is the first report on a promising gut Bdellovibrio strain against pathogenic A. hydrophila.
Topics: Aeromonas hydrophila; Animals; Antibiosis; Bacteria; Bdellovibrio; Biological Control Agents; Fish Diseases; Gram-Negative Bacterial Infections; Phylogeny; Polymerase Chain Reaction
PubMed: 21864996
DOI: 10.1016/j.vetmic.2011.07.032 -
Clinical Microbiology and Infection :... Sep 2012A plasmid (pP2G1), which confers multidrug resistance in an environmental Aeromonas species, was completely sequenced using a shotgun approach. Plasmid pP2G1 encoded...
A plasmid (pP2G1), which confers multidrug resistance in an environmental Aeromonas species, was completely sequenced using a shotgun approach. Plasmid pP2G1 encoded resistance to aminoglycosides and quinolones [aac(6')-Ib-cr], β-lactams (bla(OXA-1)), chloramphenicol (catB3), macrolides [mphA-mrx-mphR], quaternary ammonium compounds (qacEΔ1), quinolones (qnrS2), rifampicin (arr-3) and sulphonamides (sul1). These findings suggest that Aeromonas species may potentially act as reservoirs of antibiotic resistance genes.
Topics: Aeromonas; Drug Resistance, Multiple, Bacterial; Microbial Sensitivity Tests; Plasmids; Rivers; Spain; Water Microbiology
PubMed: 22725683
DOI: 10.1111/j.1469-0691.2012.03935.x -
The Journal of Physical Chemistry... Sep 2021Antifreeze proteins (AFPs) can bind to ice nuclei thereby inhibiting their growth and their hydration shell is believed to play a fundamental role. Here, we use...
Antifreeze proteins (AFPs) can bind to ice nuclei thereby inhibiting their growth and their hydration shell is believed to play a fundamental role. Here, we use molecular dynamics simulations to characterize the hydration shell of four moderately-active and four hyperactive AFPs. The local water density around the ice-binding-surface (IBS) is found to be lower than that around the non-ice-binding surface (NIBS) and this difference correlates with the higher hydrophobicity of the former. While the water-density increase (with respect to bulk) around the IBS is similar between moderately-active and hyperactive AFPs, it differs around the NIBS, being higher for the hyperactive AFPs. We hypothesize that while the lower water density at the IBS can pave the way to protein binding to ice nuclei, irrespective of the antifreeze activity, the higher density at the NIBS of the hyperactive AFPs contribute to their enhanced ability in inhibiting ice growth around the bound AFPs.
Topics: Aeromonadaceae; Antifreeze Proteins; Bacterial Proteins; Basidiomycota; Crystallization; Granulovirus; Hydrophobic and Hydrophilic Interactions; Ice; Isomerism; Molecular Dynamics Simulation; Protein Binding; Protein Conformation; Surface Properties; Temperature
PubMed: 34491750
DOI: 10.1021/acs.jpclett.1c01855 -
Viruses Nov 2022is a zoonotic pathogen and an important fish pathogen. A new lytic phage, Ahy-yong1, against multi-antibiotic-resistant pathogen was isolated, identified, and...
is a zoonotic pathogen and an important fish pathogen. A new lytic phage, Ahy-yong1, against multi-antibiotic-resistant pathogen was isolated, identified, and tentatively used in therapy. Ahy-yong1 possesses a head of approximately 66 nm in diameter and a short tail of approximately 26 nm in length and 32 nm in width. Its complete dsDNA genome is 43,374 bp with a G + C content of 59.4%, containing 52 predicted opening reading frames (ORFs). Taxonomic analysis indicated Ahy-yong1 as a new species of the genus of the family of the class. Ahy-yong1 was active only against its indicator host strain among the 35 strains tested. It is stable at 30-40 °C and at pH 2-12. phage Ahy-yong1 revealed an effective biofilm removal capacity and an obvious protective effect in brocade carp ( Koi). The average cumulative mortality for the brocade carp in the blank groups intraperitoneally injected with PBS was 1.7% ± 2.4%;for the control groups treated with (10 CFU/fish) via intraperitoneal injection, it was 100.00%;and for the test group I, successively treated with (10 CFU/fish) and phage Ahy-yong1 (10 PFU/fish) via intraperitoneal injection witha time interval of 2 hours, it was only 43.4% ± 4.7%. Furthermore, the cumulative mortality of the test group II, successively treated with phage Ahy-yong1 (10 PFU/fish) and (10 CFU/fish), was only 20.0% ± 8.2%, and that of the test group III, simultaneously treated with phage Ahy-yong1 (10 PFU/fish) and (10 CFU/fish), was only 30.0% ± 8.2%. The results demonstrated that phage Ahy-yong1 was very effective in the therapies against A18, prophylaxis was more effective than rescue, and earlier treatment was better for the reduction of mortality. This study enriches knowledge about phages.
Topics: Animals; Aeromonas hydrophila; Aeromonas; Carps; Bacteriophages; Caudovirales
PubMed: 36423108
DOI: 10.3390/v14112498