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Archives of Dermatological Research 1985The activities of two histamine-metabolizing enzymes, histamine-N-methyltransferase (HMT) and diamine oxidase (DAO), were examined in various types of experimentally... (Comparative Study)
Comparative Study
The activities of two histamine-metabolizing enzymes, histamine-N-methyltransferase (HMT) and diamine oxidase (DAO), were examined in various types of experimentally induced cutaneous inflammations in guinea pigs. In intact guinea-pig skin, the specific activities of HMT and DAO were 24.8 +/- 1.7 pmol/min per milligram of protein or 0.930 +/- 0.097 pmol/min per milligram of the wet weight of skin specimen, and 6.0 +/- 0.7 pmol/min per milligram of protein or 0.189 +/- 0.011 pmol/min per milligram of the wet weight, respectively. Both enzyme activities were markedly reduced in skin lesions of the Arthus reaction (P less than 0.005), while those in dinitrochlorobenzene allergic dermatitis, croton-oil dermatitis, and the intact areas in Arthus-reaction-induced animals were almost within the normal limits. The activity of HMT decreased linearly with time from the onset of the Arthus reaction, reaching about 20% of the control activity at 48 h; the activity of DAO decreased even from the early stages of the reaction, and this decrease continued throughout first 48 h of the reaction. These results suggest that impaired histamine metabolism in the skin lesions of the reaction plays a distinct role in the formation and development of the Arthus reaction.
Topics: Amine Oxidase (Copper-Containing); Animals; Arthus Reaction; Dermatitis, Contact; Female; Guinea Pigs; Histamine; Histamine N-Methyltransferase; Male; Methyltransferases; Skin
PubMed: 3159351
DOI: 10.1007/BF00509087 -
Acta Allergologica 1955
The influence of the thyroid gland on hypersensitivity reactions in animals. III. The influence of thyroidectomy on the precipitin reaction, the Arthus phenomenon and the Schultz-Dale reaction in guinea-pigs.
Topics: Animals; Arthus Reaction; Guinea Pigs; Hypersensitivity; Immune System Diseases; Precipitins; Thyroid Gland; Thyroidectomy; Viscera
PubMed: 14349516
DOI: No ID Found -
British Journal of Pharmacology Jul 1976
Comparative Study
Topics: Analgesics; Animals; Arthus Reaction; Guinea Pigs; Sulfinpyrazone; Thrombocytopenia
PubMed: 135589
DOI: No ID Found -
Lancet (London, England) Oct 1977
Topics: Aged; Arthritis, Rheumatoid; Arthus Reaction; Drug Hypersensitivity; Female; Humans; Hypersensitivity, Immediate; Levamisole
PubMed: 72274
DOI: 10.1016/s0140-6736(77)90868-6 -
Journal of Leukocyte Biology Feb 1996The CD11/CD18 leukocyte integrins are necessary for tissue localization of neutrophils, an early requisite event in inflammation. We have analyzed the contribution of... (Comparative Study)
Comparative Study
The CD11/CD18 leukocyte integrins are necessary for tissue localization of neutrophils, an early requisite event in inflammation. We have analyzed the contribution of CD11a/CD18 and CD11b/CD18 to local neutrophil accumulation and tissue injury in the reverse passive Arthus reaction in the rat dermis. Experimental groups comprised animals that received an intravenous infusion of (1) recombinant neutrophil inhibitory factor (NIF), a hookworm-derived antagonist of CD11b/CD18; (2) monoclonal antibody to CD11a/CD18 (TA-3); (3) a combination of these agents; (4) a monoclonal antibody to CD18 (WT.3); or (5) saline. Administration of recombinant NIF or anti-CD11a/CD18 monoclonal antibody alone produced a slight reduction in neutrophil accumulation but did not affect edema formation. In contrast, a combination of these antagonists yielded a significant reduction in neutrophil accumulation and a modest reduction in edema, equivalent to levels observed with either anti-CD18 antibodies or animals that were rendered neutropenic. These results indicate that neutrophil infiltration in rat dermal tissue in the reverse passive Arthus reaction is dependent predominantly on the leukocyte integrins CD11a/CD18 and CD11b/CD18 and that either of these integrins is sufficient for neutrophil trafficking in this inflammatory setting.
Topics: Animals; Antibodies, Monoclonal; Arthus Reaction; CD11 Antigens; CD18 Antigens; CHO Cells; Cricetinae; Dermatitis; Edema; Glycoproteins; Helminth Proteins; Male; Membrane Proteins; Neutrophils; Rabbits; Rats; Recombinant Proteins
PubMed: 8603998
DOI: 10.1002/jlb.59.2.254 -
Clinical and Experimental Dermatology Dec 2016Tumour necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) is a pro-inflammatory cytokine, which is closely associated with the pathogenesis of various types of...
BACKGROUND
Tumour necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) is a pro-inflammatory cytokine, which is closely associated with the pathogenesis of various types of cutaneous vasculitis (CV).
AIM
To investigate the therapeutic effects of an anti-TWEAK monoclonal antibody (mAb) in a mouse model of cutaneous reverse passive Arthus (RPA) reaction.
METHODS
Cutaneous RPA reaction was induced in BALB/c mice by intradermal injection of anti-ovalbumin IgG into the left ear followed immediately by intravenous injection of chicken ovalbumin. After treatment, haemorrhagic lesions in the mouse skin were scored semiquantitatively. The amount of extravasated fluorescein isothiocyanate (FITC)-labelled bovine serum albumin (BSA) in the ears was detected spectrophotometrically. Expression of myeloperoxidase (MPO) was detected by immunohistochemical staining, while mRNA expression of TNF-α and interleukin (IL)-6 in lesional skin was detected by real-time quantitative (q)PCR.
RESULTS
Our results indicated that anti-TWEAK mAb significantly attenuated the clinical and histopathological changes in immune complex (IC)-induced mice, and also reduced the semiquantitative haemorrhage score, FITC-labelled BSA extravasation and MPO activity. Real-time qPCR showed that anti-TWEAK mAb significantly inhibited mRNA expression of TNF-α and IL-6 in lesional skin from IC-induced mice.
CONCLUSION
These data suggest that anti-TWEAK mAb can block vascular damage and leucocyte infiltration in IC-induced mice. TWEAK might be a candidate immunotherapeutic medicine for suppression of IC-induced CV.
Topics: Animals; Antibodies, Monoclonal; Arthus Reaction; Cytokine TWEAK; Cytokines; Disease Models, Animal; Male; Mice; Mice, Inbred BALB C; Peroxidase; Real-Time Polymerase Chain Reaction; Skin Diseases
PubMed: 27753135
DOI: 10.1111/ced.12912 -
The British Journal of Ophthalmology May 2001The Arthus type allergic reaction is characterised by inflammatory cell infiltration and marked neovascularisation in the cornea. During the healing stages, inflammatory...
BACKGROUND/AIMS
The Arthus type allergic reaction is characterised by inflammatory cell infiltration and marked neovascularisation in the cornea. During the healing stages, inflammatory cells and newly formed microvessels gradually disappear. The aim was to establish whether apoptosis affected the regression of inflammatory cells and newly formed microvessels, in order to define more clearly the cellular mechanisms involved in the pathobiology of corneal diseases.
METHODS
Albino male rabbits were injected subcutaneously with 5 mg/ml bovine serum albumin (BSA) incorporated in Freund's complete adjuvant twice weekly. Under the anaesthesia, 30 microl of a 0.5 mg/ml BSA solution was injected into the central corneal stroma to induce an Arthus type allergic reaction. The injured corneas were collected at various time points ranging from 3 to 20 days. Apoptotic cells were identified by both light microscopy using in situ TdT-dUTP nick end labelling (TUNEL) method and electron microscopy.
RESULTS
With increasing time after induction of the Arthus reaction, marked neovascularisation and infiltrated inflammatory cells such as polymorphonuclear cells (PMNs) and plasma cells were observed in the cornea. Thereafter, the inflammatory cells and newly formed microvessels gradually disappeared. Coincidently, the numbers of microvessel endothelial cells and infiltrated inflammatory cells undergoing apoptosis were increased. Apoptotic bodies were taken up by macrophages, PMNs, as well as myofibroblasts derived presumably from transformation of migrated keratocytes.
CONCLUSIONS
These data demonstrate that regression of the cellular infiltrates and microvessel endothelial cells associated with the Arthus reaction in the cornea occurs via apoptosis. This finding adds insights into the cellular mechanisms regulating the pathobiology of corneal diseases.
Topics: Animals; Apoptosis; Arthus Reaction; Cornea; Corneal Neovascularization; In Situ Nick-End Labeling; Macrophages; Male; Microscopy, Electron; Microscopy, Fluorescence; Neutrophil Infiltration; Phagocytosis; Rabbits; Remission, Spontaneous
PubMed: 11316727
DOI: 10.1136/bjo.85.5.613 -
The British Journal of Dermatology Sep 1974
Topics: Adolescent; Adult; Antibodies, Bacterial; Arthus Reaction; Child; Complement System Proteins; Gangrene; Humans; Hypersensitivity, Delayed; Leg Ulcer; Lupus Vulgaris; Middle Aged; Mycobacterium tuberculosis; Tuberculosis, Cutaneous
PubMed: 4279670
DOI: 10.1111/j.1365-2133.1974.tb12894.x -
Immunopharmacology Oct 1997The effect of the histamine H2-receptor antagonist, cimetidine, on the cutaneous Arthus-like hypersensitivity to oxazolone elicited injecting subcutaneously oxazolone...
The effect of the histamine H2-receptor antagonist, cimetidine, on the cutaneous Arthus-like hypersensitivity to oxazolone elicited injecting subcutaneously oxazolone conjugated to egg-albumin (EA-OX) has been examined in the chicken. Cimetidine had opposite effects on the cutaneous reaction to oxazolone in relation to a different immunization schedule. Cimetidine enhanced the cutaneous reaction to oxazolone obtained immunizing chickens with oxazolone dissolved in ethanol (Eth-OX); instead cimetidine inhibited the cutaneous reaction obtained in chickens immunized with oxazolone dissolved in complete Freund adjuvant (CFA-OX). Optimum enhancement of the cutaneous arthus-like reaction to oxazolone occurred when cimetidine was given for three consecutive days starting at the immunization. The enhancing effect was absent in neonatally bursectomized chickens. Moreover, cimetidine stimulated bursal cell proliferation at day 1 after sensitization. The study of the immunoglobulin class of oxazolone antibodies produced in the immunized chickens demonstrated that cimetidine stimulated the IgM oxazolone antibody synthesis in Eth-OX immunized chickens and inhibited the IgY oxazolone antibody production in Eth-OX and total oxazolone antibody production in CFA-OX immunized chickens. The relationship between increased IgM oxazolone antibody synthesis and enhancement of the cutaneous Arthus reaction is discussed. The role of IgM antibodies in the pathogenesis of Arthus reaction in the chicken is hypothesized.
Topics: Adjuvants, Immunologic; Albumins; Animals; Antibody Formation; Arthus Reaction; B-Lymphocytes; Bursa of Fabricius; Chickens; Cimetidine; Dermatitis, Contact; Ethanol; Freund's Adjuvant; Histamine H2 Antagonists; Lymphocyte Activation; Male; Oxazolone
PubMed: 9403346
DOI: 10.1016/s0162-3109(97)00082-9 -
Arzneimittel-Forschung 1982The action of several steroidal and non-steroidal antiinflammatory agents, immunosuppressives, and antirheumatics (levamisole, chloroquine, sodium aurothiopropanol... (Comparative Study)
Comparative Study
The action of several steroidal and non-steroidal antiinflammatory agents, immunosuppressives, and antirheumatics (levamisole, chloroquine, sodium aurothiopropanol sulphonate, D-penicillamine) was studied in two models of Arthus-passive reaction in the rat: paw oedema induced by an anti-ovalbumin serum, and pleurisy induced by an anti-bovine-albumin serum. The steroidal antiinflammatory agents reduced both types of reaction. In pleurisy, they acted on exudate and the number of neutrophils. The non-steroidal compounds were not active on the inflammatory reaction of the paw, but they decreased the volume of exudate in pleurisy without having any clear effect on cellular phenomena. The antirheumatics and immunosuppressives showed little or no action on the two models: of these only gold salt decreased Arthus reaction in the paw.
Topics: Animals; Anti-Inflammatory Agents; Arthus Reaction; Disease Models, Animal; Edema; Glucocorticoids; Immunosuppressive Agents; Male; Pleurisy; Rabbits; Rats; Rats, Inbred Strains
PubMed: 6460508
DOI: No ID Found