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The Journal of Clinical Investigation Aug 1964
Topics: Adolescent; Beta-Globulins; Blood Urea Nitrogen; Child; Complement System Proteins; Hematuria; Humans; Immunoelectrophoresis; Liver Diseases; Lupus Erythematosus, Systemic; Nephritis; Nephrosis; Nephrosis, Lipoid; Proteinuria; Purpura; Pyelonephritis
PubMed: 14201535
DOI: 10.1172/JCI105027 -
Comparative Biochemistry and... 19821. Heterozygotes TfF/TfE of voles did not differ in body weight, they had higher hematocrit value (males and females), higher beta-globulin fraction (males) or higher... (Comparative Study)
Comparative Study
Variability of hematocrit value, blood serum beta- and gamma-globulin level and body weight in different transferrin genotypes of common vole (Microtus arvalis, Pallas 1779) from natural population.
1. Heterozygotes TfF/TfE of voles did not differ in body weight, they had higher hematocrit value (males and females), higher beta-globulin fraction (males) or higher gamma-globulin fraction (females). 2. A smaller variability of parameters studied was observed in heterozygotes than in homozygotes. Small and similar variability occurred in hematocrit value and beta-globulins while higher variability occurred in body weight and gamma-globulins. 3. In common voles the faster migrating E system of transferrin may have a higher capacity for iron transport than the F system. 4. Seasonal changes of hematocrit value in vole populations seems to result from genetical and physiological variability. 5. Sexual and seasonal changes in beta-globulin level appear to be related to changes in sexual activity of the animals during the seasons. 6. In autumn, low weight of animals and high serum gamma-globulin level, with the same physiological variability of these two parameters, suggest the synthesis of gamma-globulins with tissue (muscle) proteins. 7. Autumnal increase of gamma-globulin level seems to be very important, taking into consideration the role of the protein in water retention and its filtration from the tissues. Very high level of the protein fraction may play a role in winter high mortality of voles.
Topics: Animals; Arvicolinae; Beta-Globulins; Body Weight; Female; Genetic Variation; Genotype; Hematocrit; Male; Seasons; Sex Factors; Transferrin; gamma-Globulins
PubMed: 6181932
DOI: 10.1016/0300-9629(82)90100-1 -
International Journal of Biological... Dec 1992The investigation of the effect of acid pH on the structure of beta-globulin indicated several transitions as a function of pH. Upon reducing the pH from 7.0, the...
The investigation of the effect of acid pH on the structure of beta-globulin indicated several transitions as a function of pH. Upon reducing the pH from 7.0, the beta-globulin molecule underwent an expansion due to hydration up to pH 5.0, and a further increase in H+ concentration resulted in unfolding. This is a single step cooperative denaturation as indicated by the viscosity profile. At extreme acid pH values (below pH 2.0) the protein associates or folds to a different conformational motif as shown by blue shift of ultraviolet fluorescence emission maximum and decrease in reduced viscosity values by more than 30% due to an entropically driven hydrophobic interaction. The conformational analysis of beta-globulin showed a decrease up to pH 3.0, followed by an increase in the ordered structure at low pH values indicating that the low pH values stabilized this new conformation. These results are discussed in view of the molten globule structure of proteins.
Topics: Beta-Globulins; Chromatography, Gel; Circular Dichroism; Hydrogen-Ion Concentration; Plant Proteins; Protein Conformation; Solutions; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Ultracentrifugation; Viscosity
PubMed: 1282362
DOI: 10.1016/s0141-8130(05)80069-8 -
The Journal of Clinical Endocrinology... Dec 1966
Topics: Beta-Globulins; Blood Protein Electrophoresis; Estradiol; Estrogens; Estrone; Female; Humans; Male; Protein Binding; Tritium
PubMed: 4163699
DOI: 10.1210/jcem-26-12-1399 -
The Journal of Allergy and Clinical... Nov 2004The increased consumption of foods containing sesame seeds is paralleled by an increase in reported sesame-induced allergic reactions.
BACKGROUND
The increased consumption of foods containing sesame seeds is paralleled by an increase in reported sesame-induced allergic reactions.
OBJECTIVE
This study aimed at identifying and characterizing the linear B-cell epitopes of the 14-kd beta-globulin, the major allergen of sesame seed.
METHODS
A peptide containing 71 amino acids (peptide B) was previously identified by us as the IgE-binding region on beta-globulin. To determine the amino acid sequence of the IgE-binding sites on peptide B, we synthesized overlapping peptides 20 and 10 amino acid residues long that span the entire length of peptide B, which were offset from each other by 10 and 2 amino acid residues, respectively. Sera from 20 subjects given diagnoses of allergy to sesame beta-globulin served to identify the epitopes by using the dot-blot test.
RESULTS
At least 9 different IgE-recognition sites were identified on peptide B. Three of them, numbers 2, 3, and 13 (corresponding to amino acids 46-55, 48-57, and 76-86, respectively, in the beta-globulin sequence), appeared to be immunodominant IgE-binding epitopes. Also, these peptides were best recognized in terms of intensity of response. There was no obvious sequence motif shared by the 9 different IgE-binding epitopes of beta-globulin. However, approximately 60% of the amino acids represented in the epitopes are hydrophobic residues.
CONCLUSION
Identification of the IgE-binding epitopes might provide a better understanding of the functional role the allergens play in the disease and might have implications for immunodiagnosis and probably immunotherapy.
Topics: Adolescent; Adult; Allergens; Amino Acid Sequence; Beta-Globulins; Child; Child, Preschool; Epitopes, B-Lymphocyte; Humans; Immunoglobulin E; Infant; Molecular Sequence Data; Seeds; Sesamum
PubMed: 15536424
DOI: 10.1016/j.jaci.2004.07.038 -
Nature Oct 1963
Topics: Aluminum Silicates; Beta-Globulins; Chemical Phenomena; Chemistry; Globulins; Immunoelectrophoresis; Research; Serum Globulins
PubMed: 14081068
DOI: 10.1038/200262a0 -
Clinica Chimica Acta; International... Jan 1959
Topics: Beta-Globulins; Globulins; Serum Globulins
PubMed: 13629856
DOI: 10.1016/0009-8981(59)90088-9 -
Journal of Cell Science Aug 1980Evidence is presented to show the existence in human beta-globulin (Cohn fraction III) of 2 growth-promoting factors that stimulate DNA synthesis and cell division in...
Evidence is presented to show the existence in human beta-globulin (Cohn fraction III) of 2 growth-promoting factors that stimulate DNA synthesis and cell division in human lymphocytes and 3T3 B mouse fibroblasts. After extraction of human beta-globulins at pH 3.0 in 0.1 M NaCl followed by sieve chromatography on Sephadex, 2 distinct fractions were obtained containing the biological activity; one of polypeptide nature and molecular weight of approx. 10 000 Daltons and the other consisting of a homogeneous ribonucleic acid, as revealed by polyacrylamide gel electrophoresis. The above-mentioned factors appear to lack both cell and species specificity, since they are mitogenic agents for cells as diverse as mouse fibroblasts and human lymphocytes.
Topics: Animals; Beta-Globulins; Cell Division; Cells, Cultured; Chromatography, Gel; Dose-Response Relationship, Drug; Fibroblasts; Hot Temperature; Humans; Lymphocytes; Mice; Mitogens; Stimulation, Chemical
PubMed: 6160164
DOI: 10.1242/jcs.44.1.285 -
Arthritis and Rheumatism Apr 1964
Topics: Antibodies; Antibodies, Antinuclear; Antigens; Beta-Globulins; DNA; Fluorescent Antibody Technique; Humans; Immune Sera; Lupus Erythematosus, Systemic; Nephritis; Neutrophils; Rheumatoid Factor; Serum Globulins; gamma-Globulins
PubMed: 14150745
DOI: 10.1002/art.1780070208 -
Nature Oct 1958
Topics: Animals; Beta-Globulins; Globulins; Phenotype; Serum Globulins; Sheep
PubMed: 13590244
DOI: 10.1038/1821101a0