-
Nature Aug 1958
Topics: Beta-Globulins; Globulins; Serum Globulins
PubMed: 13577875
DOI: 10.1038/182452a0 -
Lancet (London, England) Sep 1958
Topics: Beta-Globulins; Globulins; Serum Globulins
PubMed: 13576877
DOI: No ID Found -
The Journal of Experimental Medicine Aug 1965At least 3 complement factors were found necessary for the conversion of the thermolabile intermediate complex EAC'1a,4,2a to a thermostable state. One of these factors...
At least 3 complement factors were found necessary for the conversion of the thermolabile intermediate complex EAC'1a,4,2a to a thermostable state. One of these factors is the earlier described beta(1C)-globulin. The second, a heretofore unrecorded serum protein, beta(1F)-globulin. The third factor has not yet been defined as a discrete serum protein entity. Kinetic experiments indicated that beta(1C) reacted prior to beta(1F), which in turn seemed to precede the third factor in the reaction sequence. Therefore, the 3 components were tentatively designated the third (C'3), the fifth (C'5), and the sixth (C'6) components of complement, respectively. A procedure was developed allowing the isolation of highly purified beta(1C)-(C'3) and beta(1F)-globulin (C'5) and of partially purified C'6. With respect to its function in immune hemolysis, beta(1F)-globulin or C'5 was found to be closely dependent on the simultaneous presence of C'6. The hypothesis that C'5 and C'6 form a functional unit was supported by the finding that both components interact with each other in solution resulting in the formation of a complex. A similar complex was also found in fresh human serum.
Topics: Beta-Globulins; Blood Proteins; Chromatography; Complement System Proteins; Hemolysis; Humans; Immunoelectrophoresis; Iodine Isotopes; Kinetics; Ultracentrifugation
PubMed: 14316946
DOI: 10.1084/jem.122.2.277 -
Immunology Mar 1967By the method of antigen—antibody crossed electrophoresis where the antigens after a first separation in agarose gel are driven into a new antibody containing gel,...
By the method of antigen—antibody crossed electrophoresis where the antigens after a first separation in agarose gel are driven into a new antibody containing gel, serum β-globulin and its conversion products were analysed in native serum as well as after addition of EDTA, incubation with immunoprecipitate and hydrazine, and after inactivation at 56°. It is shown that β-globulin can be converted not only to β-globulin or components with the same mobility as β-globulin but also to additional components, one in the α-region, one in the inter-β-region and one in the γ-region. α- and inter-β-components are seen especially in serum to which EDTA has been added. EDTA did not inhibit the spontaneous conversion of β-globulin.
Topics: Antigen-Antibody Reactions; Beta-Globulins; Edetic Acid; Electrophoresis; Gels; Humans; Hydrazines; In Vitro Techniques
PubMed: 4164033
DOI: No ID Found -
Lancet (London, England) Dec 1964
Topics: Alpha-Globulins; Beta-Globulins; Blood Protein Disorders; Blood Protein Electrophoresis; Blood Proteins; Diagnosis; Humans; Sarcoidosis; Skin Tests; Statistics as Topic; gamma-Globulins
PubMed: 14224034
DOI: 10.1016/s0140-6736(64)91105-5 -
The New England Journal of Medicine Sep 1965
Topics: Adolescent; Beta-Globulins; Biopsy; Child; Clinical Laboratory Techniques; Fluorescent Antibody Technique; Glomerulonephritis; Humans; Immunochemistry; Infant; Nephrotic Syndrome; Pathology; Proteinuria
PubMed: 14324514
DOI: 10.1056/NEJM196509022731004 -
Revista Espanola de Anestesiologia Y... Jul 1976
Topics: Beta-Globulins; Blood Proteins; Body Weight; Curare; Female; Humans; Hysterectomy; Immunoglobulins; Laparotomy; Protein Binding
PubMed: 61605
DOI: No ID Found -
Immunochemistry Jun 1964
Topics: Alpha-Globulins; Antigen-Antibody Reactions; Beta-Globulins; Complement Fixation Tests; Complement System Proteins; Immunoelectrophoresis; Research
PubMed: 14249322
DOI: 10.1016/0019-2791(64)90073-4 -
Vox Sanguinis 1964
Topics: Beta-Globulins; Complement System Proteins; Globulins; Immune Sera; Research; Serum Globulins
PubMed: 14115416
DOI: 10.1111/j.1423-0410.1964.tb03777.x -
Biochimica Et Biophysica Acta Feb 1975A protein fraction was isolated from the supernatant of thrombin-aggregated washed human platelets and was shown, by immunodiffusion techniques, to contain a...
The isolation and characterisation of a platelet-specific beta-globulin (beta-thromboglobulin) and the detection of antiurokinase and antiplasmin released from thrombin-aggregated washed human platelets.
A protein fraction was isolated from the supernatant of thrombin-aggregated washed human platelets and was shown, by immunodiffusion techniques, to contain a platelet-specific beta-globulin (beta-thromboglobulin) as the major component. A molecular weight of 35 800 was determined for beta-thromboglobin from the measured sedimentation coefficient of3.0 S and Stokes radius of 2.85 nm. Beta-Thromboglobin was detected in the serum from whole blood and the supernatant of 48-h-old platelet-rich plasma and 28-day-old citrated whole blood, but not in platelet-poor plasma. The fraction containing beta-thromboglobulin was shown to possess an antiurokinase activity but was devoid of antiplasmin activity. A further fraction of approximate molecular weight 70 000 was also isolated which contained an antiplasmin but was devoid of antiurokinase activity.
Topics: Animals; Antifibrinolytic Agents; Beta-Globulins; Blood Platelets; Centrifugation, Density Gradient; Chromatography, Gel; Endopeptidases; Fibrinolysin; Humans; Immune Sera; Immunodiffusion; Molecular Weight; Platelet Aggregation; Rabbits; Time Factors; Ultrafiltration; Urokinase-Type Plasminogen Activator
PubMed: 47244
DOI: 10.1016/0005-2795(75)90143-9