-
C-Jun N-terminal kinase (JNK) pathway activation is essential for dental papilla cells polarization.PloS One 2021During tooth development, dental papilla cells differentiate into odontoblasts with polarized morphology and cell function. Our previous study indicated that the C-Jun...
During tooth development, dental papilla cells differentiate into odontoblasts with polarized morphology and cell function. Our previous study indicated that the C-Jun N-terminal kinase (JNK) pathway regulates human dental papilla cell adhesion, migration, and formation of focal adhesion complexes. The aim of this study was to further examine the role of the JNK pathway in dental papilla cell polarity formation. Histological staining, qPCR, and Western Blot suggested the activation of JNK signaling in polarized mouse dental papilla tissue. After performing an in vitro tooth germ organ culture and cell culture, we found that JNK inhibitor SP600125 postponed tooth germ development and reduced the polarization, migration and differentiation of mouse dental papilla cells (mDPCs). Next, we screened up-regulated polarity-related genes during dental papilla development and mDPCs or A11 differentiation. We found that Prickle3, Golga2, Golga5, and RhoA were all up-regulated, which is consistent with JNK signaling activation. Further, constitutively active RhoA mutant (RhoA Q63L) partly rescued the inhibition of SP600125 on cell differentiation and polarity formation of mDPCs. To sum up, this study suggests that JNK signaling has a positive role in the formation of dental papilla cell polarization.
Topics: Animals; Anthracenes; Cell Differentiation; Cell Movement; Cell Polarity; Cells, Cultured; Dental Papilla; JNK Mitogen-Activated Protein Kinases; MAP Kinase Signaling System; Mice; Mice, Inbred ICR; Mutagenesis; Tooth Germ; rhoA GTP-Binding Protein
PubMed: 33770099
DOI: 10.1371/journal.pone.0233944 -
Dental Clinics of North America Jul 2012The search for more accessible mesenchymal stem cells than those found in bone marrow has propelled interest in dental tissues. Human dental stem/progenitor cells... (Review)
Review
The search for more accessible mesenchymal stem cells than those found in bone marrow has propelled interest in dental tissues. Human dental stem/progenitor cells (collectively termed dental stem cells [DSCs]) that have been isolated and characterized include dental pulp stem cells, stem cells from exfoliated deciduous teeth, stem cells from apical papilla, periodontal ligament stem cells, and dental follicle progenitor cells. Common characteristics of these cell populations are the capacity for self-renewal and the ability to differentiate into multiple lineages. In vitro and animal studies have shown that DSCs can differentiate into osseous, odontogenic, adipose, endothelial, and neural-like tissues.
Topics: Cell Differentiation; Cell Lineage; Dental Papilla; Dental Pulp; Dental Sac; Guided Tissue Regeneration; Humans; Mesenchymal Stem Cells; Periodontal Ligament; Pluripotent Stem Cells
PubMed: 22835537
DOI: 10.1016/j.cden.2012.05.004 -
Oral Diseases Jan 2014In addition to their well-established self-renewal and multipotent differentiation properties, mesenchymal stem cells (MSCs) also possess potent immunomodulatory... (Review)
Review
In addition to their well-established self-renewal and multipotent differentiation properties, mesenchymal stem cells (MSCs) also possess potent immunomodulatory functions both in vitro and in vivo, which render them a potential novel immunotherapeutic tool for a variety of autoimmune and inflammation-related diseases. The major mechanisms may involve (1) the secretion of an array of soluble factors such as prostaglandin E2 (PGE2 ), indoleamine 2, 3-dioxygenase (IDO), transforming growth factor-β (TGF-β), and human leukocyte antigen G5 (HLA-G5); (2) interactions between MSCs and immune cells such as T cells, B cells, macrophages, and dendritic cells. Recently, increasing evidence has supported that MSCs derived from dental tissues are promising alternative sources of multipotent MSCs. We here provide a thorough and extensive review about new findings in the immunomodulatory functions of MSCs derived from several dental tissues, including dental pulp, periodontal ligament, gingiva, exfoliated deciduous teeth, apical papilla, and dental follicle, respectively. The immunomodulatory properties of dental MSCs place them as a more accessible cell source than bone marrow-derived MSCs for cell-based therapy of immune and inflammation-related diseases.
Topics: Dental Papilla; Dental Pulp; Dental Sac; Gingiva; Humans; Mesenchymal Stem Cells; Periodontal Ligament; Tooth, Deciduous
PubMed: 23463961
DOI: 10.1111/odi.12086 -
Stem Cells and Development Jul 2015Although regenerative endodontic procedures have yielded an impressive body of favorable outcomes, the treatment of necrotic immature permanent teeth in particular... (Review)
Review
Although regenerative endodontic procedures have yielded an impressive body of favorable outcomes, the treatment of necrotic immature permanent teeth in particular remains to be a challenge. Recent advances in dental stem cell (DSC) research have gained increasing insight in their regenerative potential and prospective use in the formation of viable dental tissues. Numerous studies have already reported successful dental pulp regeneration following application of dental pulp stem cells, stem cells from the apical papilla, or dental follicle precursor cells in different in vivo models. Next to responsive cells, dental tissue engineering also requires the support of an appropriate scaffold material, ranging from naturally occurring polymers to treated dentin matrix components. However, the routine use and banking of DSCs still holds some major challenges, such as culture-associated differences, patient-related variability, and the effects of culture medium additives. Only in-depth evaluation of these problems and the implementation of standardized models and protocols will effectively lead to better alternatives for patients who no longer benefit from current treatment protocols.
Topics: Dental Papilla; Dental Pulp; Dental Sac; Endodontics; Guided Tissue Regeneration; Humans; Neovascularization, Physiologic; Stem Cells; Tissue Engineering; Tissue Scaffolds
PubMed: 25869156
DOI: 10.1089/scd.2014.0510 -
Expert Opinion on Biological Therapy Feb 2018Human dental stem cells can be obtained from postnatal teeth, extracted wisdom teeth or exfoliated deciduous teeth. Due to their differentiation potential, these... (Review)
Review
INTRODUCTION
Human dental stem cells can be obtained from postnatal teeth, extracted wisdom teeth or exfoliated deciduous teeth. Due to their differentiation potential, these mesenchymal stem cells are promising for tooth repair. Therefore, the development of dental tissue regeneration represents a suitable but challenging, target for dental stem cell therapies. Areas covered: Expert opinion:
AREAS COVERED
In this review, the authors provide an overview of human dental stem cells and their properties for regeneration medicine. Numerous preclinical studies have shown that dental stem cells improve bone augmentation and healing of periodontal diseases. Clinical trials are ongoing to validate the clinical feasibility of these approaches. Dental stem cells are also important for basic research.
EXPERT OPINION
Dental stem cells offer numerous advantages for tooth repair and regeneration. Data obtained from different studies are encouraging. In the next few years, investigations on dental stem cells in basic research, pre-clinical research and clinical studies will pave the way to optimizing patient-tailored treatments for repair and regeneration of dental tissues.
Topics: Cell Differentiation; Dental Papilla; Dental Pulp; Dental Sac; Humans; Periodontal Ligament; Regeneration; Stem Cell Transplantation; Stem Cells; Tooth
PubMed: 29110535
DOI: 10.1080/14712598.2018.1402004 -
Pediatric Dentistry Nov 2016The purposes of this case report were to describe a growing two-cm gingival mass that developed after natal teeth were extracted in a four-month-old female patient,... (Review)
Review
The purposes of this case report were to describe a growing two-cm gingival mass that developed after natal teeth were extracted in a four-month-old female patient, present a review of the literature on the growth of a gingival mass after the extraction of natal teeth, and illustrate the clinical and histological features that differentiate this condition from other types of gingival masses in infants. Histological examination of the excised mass revealed that it contained tooth-like hard tissue (regular and irregular dentin) that intermingled with bone, dental pulp, and fibrous tissue. We found eight cases from 1962 to 2009 in which a soft-tissue mass with dentin-like hard tissue or a tooth-like structure had developed after the extraction of natal teeth. Based on clinical and histological findings, we deduced that the mass was the result of abnormal growth of a residual dental papilla, including mesenchymal stem cells. Consequently, dentists, obstetricians, gynecologists, and pediatricians should be aware of this potential complication and observe caution before they extract natal teeth.
Topics: Dental Papilla; Dental Pulp; Dentin, Secondary; Female; Gingiva; Humans; Infant; Mesenchymal Stem Cells; Natal Teeth; Tooth Extraction
PubMed: 28281945
DOI: No ID Found -
Annals of Medicine Dec 2017Since the disclosure of adult mesenchymal stem cells (MSCs), there have been an intense investigation on the characteristics of these cells and their potentialities.... (Review)
Review
Since the disclosure of adult mesenchymal stem cells (MSCs), there have been an intense investigation on the characteristics of these cells and their potentialities. Dental stem cells (DSCs) are MSC-like populations with self-renewal capacity and multidifferentiation potential. Currently, there are five main DSCs, dental pulp stem cells (DPSCs), stem cells from exfoliated deciduous teeth (SHED), stem cells from apical papilla (SCAP), periodontal ligament stem cells (PDLSCs) and dental follicle precursor cells (DFPCs). These cells are extremely accessible, prevail during all life and own an amazing multipotency. In the past decade, DPSCs and SHED have been thoroughly studied in regenerative medicine and tissue engineering as autologous stem cells therapies and have shown amazing therapeutic abilities in oro-facial, neurologic, corneal, cardiovascular, hepatic, diabetic, renal, muscular dystrophy and auto-immune conditions, in both animal and human models, and most recently some of them in human clinical trials. In this review, we focus the characteristics, the multiple roles of DSCs and its potential translation to clinical settings. These new insights of the apparently regenerative aptitude of these DSCs seems quite promising to investigate these cells abilities in a wide variety of pathologies. Key messages Dental stem cells (DSCs) have a remarkable self-renewal capacity and multidifferentiation potential; DSCs are extremely accessible and prevail during all life; DSCs, as stem cells therapies, have shown amazing therapeutic abilities in oro-facial, neurologic, corneal, cardiovascular, hepatic, diabetic, renal, muscular dystrophy and autoimmune conditions; DSCs are becoming extremely relevant in tissue engineering and regenerative medicine.
Topics: Adult; Dental Papilla; Dental Pulp; Dental Sac; Humans; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; Periodontal Ligament; Regenerative Medicine; Tissue Engineering; Tooth; Tooth, Deciduous
PubMed: 28649865
DOI: 10.1080/07853890.2017.1347705 -
Seminars in Diagnostic Pathology Nov 1999The odontogenic myxoma is an uncommon tumor that has the potential for extensive bony destruction, extension into surrounding structures, and a relatively high... (Review)
Review
The odontogenic myxoma is an uncommon tumor that has the potential for extensive bony destruction, extension into surrounding structures, and a relatively high recurrence rate. Treatment often requires bone resection. The bland histologic features of a monotonous proliferation of a loose, mesenchymal fibrous tissue that lacks atypia may easily lead to a misdiagnosis. The primitive dental pulp, the dental papilla, and the tooth follicle are histologically similar to myxoma. These soft tissue fragments often separate from extracted developing teeth that are submitted to the pathologist and may easily be misinterpreted as an odontogenic myxoma. The pathologist must have good clinical and radiographic correlation to avoid a misdiagnosis and to prevent unnecessary additional surgery.
Topics: Dental Papilla; Dental Pulp; Dental Sac; Diagnostic Errors; Humans; Jaw Neoplasms; Mexico; Myxoma; Neoplasm Recurrence, Local; Odontogenic Tumors
PubMed: 10587272
DOI: No ID Found -
PeerJ 2023Dental papilla cells (DPCs) are one of the key stem cells for tooth development, eventually forming dentin and pulp. Previous studies have reported that PER2 is...
BACKGROUND
Dental papilla cells (DPCs) are one of the key stem cells for tooth development, eventually forming dentin and pulp. Previous studies have reported that PER2 is expressed in a 24-hour oscillatory pattern in DPCs . , PER2 is highly expressed in odontoblasts (which are differentiated from DPCs). However, whether PER2 modulates the odontogenic differentiation of DPCs is uncertain. This research was to identify the function of PER2 in the odontogenic differentiation of DPCs and preliminarily explore its mechanisms.
METHODS
We monitored the expression of PER2 in DPCs differentiated . We used PER2 overexpression and knockdown studies to assess the role of PER2 in DPC differentiation and performed intracellular ATP content and reactive oxygen species (ROS) assays to further investigate the mechanism.
RESULTS
PER2 expression was considerably elevated throughout the odontoblastic differentiation of DPCs . Overexpressing boosted levels of odontogenic differentiation markers, such as dentin sialophosphoprotein (), dentin matrix protein 1 (), and alkaline phosphatase (), and enhanced mineralized nodule formation in DPCs. Conversely, the downregulation of inhibited the differentiation of DPCs. Additionally, downregulating further affected intracellular ATP content and ROS levels during DPC differentiation.
CONCLUSION
Overall, we demonstrated that PER2 positively regulates the odontogenic differentiation of DPCs, and the mechanism may be related to mitochondrial function as shown by intracellular ATP content and ROS levels.
Topics: Reactive Oxygen Species; Dental Papilla; Cell Differentiation; Odontoblasts; Adenosine Triphosphate
PubMed: 38084142
DOI: 10.7717/peerj.16489 -
Schweizer Monatsschrift Fur Zahnmedizin... 2010Stem cell biology, an emerging field of research, provides promising methods in vitro as well as in vivo in animal models which make speculation about a future... (Review)
Review
Stem cell biology, an emerging field of research, provides promising methods in vitro as well as in vivo in animal models which make speculation about a future application in human dentistry reasonable. The objective of this study was to review the literature of stem cell research concerning fields relevant for dentistry. In dentistry, different stem cells are discussed. Adult dental ectomesenchymal stem cells seem promising for future therapy. Human stem cells have been isolated from the dental pulp, exfoliated deciduous teeth, the periodontal ligament, the dental follicle and the dental papilla. Stem cell markers such as STRO-1 were used for the characterization and isolation of stem cells. Adult dental stem cells can differentiate into many dental components, such as dentin, periodontal ligament, cement and dental pulp tissue, but not into enamel.
Topics: Adult Stem Cells; Animals; Antigens, Differentiation; Biomarkers; Dental Papilla; Dental Pulp; Dental Sac; Epithelial Cells; Humans; Mesenchymal Stem Cells; Odontogenesis; Periodontal Ligament; Regeneration; Tooth, Deciduous
PubMed: 21207302
DOI: No ID Found