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Colloids and Surfaces. B, Biointerfaces Apr 2021The salivary pellicle, an adlayer formed by adsorption of salivary components on teeth and dental biomaterials, has direct consequences on basic outcomes of dentistry.... (Review)
Review
The salivary pellicle, an adlayer formed by adsorption of salivary components on teeth and dental biomaterials, has direct consequences on basic outcomes of dentistry. Here, we provide an overview of salivary pellicle formation processes with a critical focus on dental biomaterials. We describe and critique the array of salivary pellicle measurement techniques. We also discuss factors that may affect salivary pellicle formation and the heterogeneity of the published literature describing salivary pellicle formation on dental biomaterials. Finally, we survey the many effects salivary pellicles have on dental biomaterials and highlight its implications on design criteria for dental biomaterials. Future investigations may lead to rationally designed dental biomaterials to control the salivary pellicle and enhance material function and patient outcomes.
Topics: Adsorption; Biocompatible Materials; Dental Pellicle; Humans; Saliva; Salivary Proteins and Peptides; Surface Properties
PubMed: 33460965
DOI: 10.1016/j.colsurfb.2021.111570 -
Archives of Oral Biology Sep 2021All soft and solid surfaces exposed to the oral cavity are covered by an acquired pellicle. While the pellicle adsorbed on enamel is well researched, only limited data... (Review)
Review
OBJECTIVE
All soft and solid surfaces exposed to the oral cavity are covered by an acquired pellicle. While the pellicle adsorbed on enamel is well researched, only limited data are available on the dentin pellicle. The purpose of the present review is to summarize studies considering the composition, structure and properties of the dentin pellicle and compare them with the current state of research on enamel pellicle.
METHODS
The literature search was conducted using Medline database and Google Scholar, including checking reference lists of journal articles by handsearching. Thereby, 19 studies were included in the present review.
RESULTS AND CONCLUSION
The dentin pellicle has a similar ultrastructure to the enamel pellicle, which is up to 1 μm thick depending on pellicle formation time and localization in the oral cavity. In contrast, due to the lack of studies on the dentin pellicle regarding its composition and properties, a comparison to the enamel pellicle is difficult. So far, only one study showed anti-abrasive properties and data on anti-erosive properties were controversial. Despite becoming more and more clinically relevant due to the increasing frequency of dentin exposure, the dentin pellicle is largely unexplored. For further investigations it is not only necessary to standardize dentin specimens, but also to assess fundamental research on dentin itself, as its complex morphology and composition may have a crucial influence on pellicle formation. Furthermore, a more detailed knowledge of the dentin pellicle may also reveal target sites for modification in favor of its protective properties.
Topics: Dental Enamel; Dental Pellicle; Dental Research; Dentin; Humans; Tooth Erosion
PubMed: 34325346
DOI: 10.1016/j.archoralbio.2021.105212 -
Archives of Oral Biology Jun 2014Dental erosion is a multifactorial condition that can result in the loss of tooth structure and function, potentially increasing tooth sensitivity. The exposure of... (Review)
Review
Dental erosion is a multifactorial condition that can result in the loss of tooth structure and function, potentially increasing tooth sensitivity. The exposure of enamel to acids from non-bacterial sources is responsible for the progression of erosion. These erosive challenges are counteracted by the anti-erosive properties of the acquired pellicle (AP), an integument formed in vivo as a result of selective adsorption of salivary proteins on the tooth surface, containing also lipids and glycoproteins. This review provides an in-depth discussion regarding how the physical structure of the AP, along with its composition, contributes to AP anti-erosive properties. The physical properties that contribute to AP protective nature include pellicle thickness, maturation time, and site of development. The pellicle contains salivary proteins embedded within its structure that demonstrate anti-erosive properties; however, rather than individual proteins, protein-protein interactions play a fundamental role in the protective nature of the AP. In addition, dietary and synthetic proteins can modify the pellicle, enhancing its protective efficiency against dental erosion. The salivary composition of the AP and its corresponding protein-profile may be employed as a diagnostic tool, since it likely contains salivary biomarkers for oral diseases that initiate at the enamel surface, including dental erosion. Finally, by modifying the composition and structure of the AP, this protein integument has the potential to be used as a target-specific treatment option for oral diseases related to tooth demineralization.
Topics: Biomarkers; Dental Pellicle; Diet; Humans; Saliva; Salivary Proteins and Peptides; Tooth Erosion
PubMed: 24731988
DOI: 10.1016/j.archoralbio.2014.02.002 -
Advances in Dental Research Dec 2000
Review
Topics: Adsorption; Amino Acids; Carbohydrates; Dental Deposits; Dental Enamel; Dental Pellicle; Humans; Saliva; Salivary Proteins and Peptides
PubMed: 11842920
DOI: 10.1177/08959374000140010301 -
Colloids and Surfaces. B, Biointerfaces Jun 2020Dental materials are susceptible to dental plaque formation, which increases the risk of biofilm-associated oral diseases. Physical-chemical properties of dental...
Dental materials are susceptible to dental plaque formation, which increases the risk of biofilm-associated oral diseases. Physical-chemical properties of dental material surfaces can affect salivary pellicle formation and bacteria attachment, but relationships between these properties have been understudied. We aimed to assess the effects of surface properties and adsorbed salivary pellicle on Streptococcus gordonii adhesion to traditional dental materials. Adsorption of salivary pellicle from one donor on gold, stainless steel, alumina and zirconia was monitored with a quartz crystal microbalance with dissipation monitoring (QCM-D). Surfaces were characterized by X-ray photoelectron spectroscopy, atomic force microscopy and water contact angles measurement before and after pellicle adsorption. Visualization and quantification of Live/Dead stained bacteria and scanning electron microscopy were used to study S. gordonii attachment to materials with and without pellicle. The work of adhesion between surfaces and bacteria was also determined. Adsorption kinetics and the final thickness of pellicle formed on the four materials were similar. Pellicle deposition on all materials increased surface hydrophilicity, surface energy and work of adhesion with bacteria. Surfaces with pellicle had significantly more attached bacteria than surfaces without pellicle, but the physical-chemical properties of the dental material did not significantly alter bacteria attachment. Our findings suggested that the critical factor increasing S. gordonii attachment was the salivary pellicle formed on dental materials. This is attributed to increased work of adhesion between bacteria and substrates with pellicle. New dental materials should be designed for controlling bacteria attachment by tuning thickness, composition and structure of the adsorbed salivary pellicle.
Topics: Adsorption; Anti-Bacterial Agents; Chemistry, Physical; Dental Materials; Dental Pellicle; Humans; Microbial Sensitivity Tests; Particle Size; Streptococcus gordonii; Surface Properties
PubMed: 32172164
DOI: 10.1016/j.colsurfb.2020.110938 -
Molecules (Basel, Switzerland) Sep 2023(1) Background: In the oral environment, sound enamel and dental restorative materials are immediately covered by a pellicle layer, which enables bacteria to attach. For...
(1) Background: In the oral environment, sound enamel and dental restorative materials are immediately covered by a pellicle layer, which enables bacteria to attach. For the development of new materials with repellent surface functions, information on the formation and maturation of salivary pellicles is crucial. Therefore, the present in situ study aimed to investigate the proteomic profile of salivary pellicles formed on different dental composites. (2) Methods: Light-cured composite and bovine enamel samples (controls) were exposed to the oral cavity for 30, 90, and 120 min. All samples were subjected to optical and mechanical profilometry, as well as SEM surface evaluation. Acquired pellicles and unstimulated whole saliva samples were analyzed by SELDI-TOF-MS. The significance was determined by the generalized estimation equation and the post-hoc bonferroni adjustment. (3) Results: SEM revealed the formation of homogeneous pellicles on all test and control surfaces. Profilometry showed that composite surfaces tend to be of higher roughness compared to enamel. SELDI-TOF-MS detected up to 102 different proteins in the saliva samples and up to 46 proteins in the pellicle. Significant differences among 14 pellicle proteins were found between the composite materials and the controls. (4) Conclusions: Pellicle formation was material- and time-dependent. Proteins differed among the composites and to the control.
Topics: Animals; Cattle; Saliva; Proteomics; Dental Pellicle; Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 37836647
DOI: 10.3390/molecules28196804 -
Monographs in Oral Science 2014All tooth surfaces exposed to the oral environment are naturally coated by the acquired salivary pellicle. The pellicle is composed of adsorbed macromolecular components... (Review)
Review
All tooth surfaces exposed to the oral environment are naturally coated by the acquired salivary pellicle. The pellicle is composed of adsorbed macromolecular components from saliva, gingival crevicular fluid, blood, bacteria, mucosa and diet. The pellicle (formed in situ/in vivo) functions as a semipermeable network of adsorbed salivary macromolecules and provides partial protection against acidic challenges; however, it cannot completely prevent demineralization of the tooth surface. The physiological pellicle reduces calcium and phosphate release from the enamel, and much less from the dentinal surface. With high probability, calcium- and phosphate-binding peptides and proteins adsorbed in the basal pellicle layer are of main relevance for the erosion-reducing effects of the natural salivary pellicle. Improvement of the pellicle's protective properties by dietary components (e.g. polyphenolic agents) might be a promising erosion-preventive approach that, however, needs validation by in situ experiments.
Topics: Adsorption; Calcium; Calcium-Binding Proteins; Dental Pellicle; Humans; Phosphate-Binding Proteins; Phosphates; Salivary Proteins and Peptides; Tooth Erosion
PubMed: 24993268
DOI: 10.1159/000360376 -
The Journal of Nihon University School... Sep 1994
Review
Topics: Bacteria, Anaerobic; Bacterial Adhesion; Dental Deposits; Dental Pellicle; Dental Plaque; Humans; Saliva
PubMed: 7989958
DOI: 10.2334/josnusd1959.36.157 -
Scientific Reports Dec 2016We aimed to comprehensively compare two compartmented oral proteomes, the salivary and the dental pellicle proteome. Systematic review and datamining was used to obtain... (Review)
Review
We aimed to comprehensively compare two compartmented oral proteomes, the salivary and the dental pellicle proteome. Systematic review and datamining was used to obtain the physico-chemical, structural, functional and interactional properties of 1,515 salivary and 60 identified pellicle proteins. Salivary and pellicle proteins did not differ significantly in their aliphatic index, hydrophaty, instability index, or isoelectric point. Pellicle proteins were significantly more charged at low and high pH and were significantly smaller (10-20 kDa) than salivary proteins. Protein structure and solvent accessible molecular surface did not differ significantly. Proteins of the pellicle were more phosphorylated and glycosylated than salivary proteins. Ion binding and enzymatic activities also differed significantly. Protein-protein-ligand interaction networks relied on few key proteins. The identified differences between salivary and pellicle proteins could guide proteome compartmentalization and result in specialized functionality. Key proteins could be potential targets for diagnostic or therapeutic application.
Topics: Animals; Data Mining; Dental Enamel Proteins; Dental Pellicle; Humans; Proteome; Salivary Proteins and Peptides
PubMed: 27966577
DOI: 10.1038/srep38882 -
Monographs in Oral Science 2014Tooth wear is the result of three processes: abrasion (wear produced by interaction between teeth and other materials), attrition (wear through tooth-tooth contact) and... (Review)
Review
Tooth wear is the result of three processes: abrasion (wear produced by interaction between teeth and other materials), attrition (wear through tooth-tooth contact) and erosion (dissolution of hard tissue by acidic substances). A further process (abfraction) might potentiate wear by abrasion and/or erosion. Knowledge of these tooth wear processes and their interactions is reviewed. Both clinical and experimental observations show that individual wear mechanisms rarely act alone but interact with each other. The most important interaction is the potentiation of abrasion by erosive damage to the dental hard tissues. This interaction seems to be the major factor in occlusal and cervical wear. The available evidence is insufficient to establish whether abfraction is an important contributor to tooth wear in vivo. Saliva can modulate erosive/abrasive tooth wear, especially through formation of pellicle, but cannot prevent it.
Topics: Dental Enamel; Dental Pellicle; Dentin; Humans; Saliva; Tooth Abrasion; Tooth Attrition; Tooth Cervix; Tooth Erosion; Tooth Wear
PubMed: 24993256
DOI: 10.1159/000359936