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Journal of Dentistry Sep 1997The objectives of this paper are to review the structure of dentine as it pertains to adhesive bonding and to describe the importance of resin permeation into dentinal... (Meta-Analysis)
Meta-Analysis
OBJECTIVES
The objectives of this paper are to review the structure of dentine as it pertains to adhesive bonding and to describe the importance of resin permeation into dentinal tubules and into spaces created between collagen fibrils by acid-etching during resin bonding. The advantages and disadvantages of separate acid-etching, priming and adhesive applications are discussed.
DATA SOURCES
Although not an exhaustive review, the concepts included in the review were obtained from the dentine bonding literature.
STUDY SELECTION
Attempts were made to critically evaluate what is known about dentine permeability and adhesion and what remains to be discovered. Speculations were made on a number of controversial issues that are not yet resolved.
CONCLUSIONS
Acid-etching of dentine produces profound changes in the chemical composition and physical properties of the matrix which can influence the quality of resin-dentine bonds, their strength and perhaps their durability.
Topics: Acid Etching, Dental; Chemical Phenomena; Chemistry, Physical; Collagen; Dental Bonding; Dental Leakage; Dentin; Dentin Permeability; Dentin-Bonding Agents; Dentinal Fluid; Humans; Methacrylates; Resin Cements; Resins, Synthetic; Smear Layer; Stress, Mechanical; Surface Properties; Water
PubMed: 9241954
DOI: 10.1016/s0300-5712(96)00057-7 -
Computer Methods in Biomechanics and... 2014Dentine is the fundamental substrate of restorative dentistry, and its properties and characteristics are the key determinants of restorative processes. In contemporary...
Dentine is the fundamental substrate of restorative dentistry, and its properties and characteristics are the key determinants of restorative processes. In contemporary restorative techniques, bonding to Dentine is created by the impregnation of the demineralised dentine by blends of resin monomers. In this paper, a numerical model of dentinal infiltration is proposed. The aim is to follow the resin front and to point out the optimal parameter set. The main tool is a level set technique to follow the evolving interface. It is coupled with the Navier-Stokes equation where capillary effect gives rise to the appearance of a new term in the variational approach than discretised by finite elements. Using an appropriate geometry representing demineralised dentine, the moving front is observed. First, a simulation of porosimetry test is achieved in order to validate the model. The two expected pore sizes are detected and the simulation also points out limitations of mercury intrusion porosimetry test in an educational way. Then a wetting fluid (representing the dental resin) is numerically infiltrated. In the dentinal porous network, capillarity is taken into account in our model by including a capillary term. A crucial conclusion is drawn from this study: resin application time by practitioners is sufficient if, in the infiltration process, the wetting phase is the resin.
Topics: Collagen; Dental Bonding; Dentin; Finite Element Analysis; Humans; Mercury; Models, Anatomic; Porosity; Resins, Synthetic
PubMed: 22871161
DOI: 10.1080/10255842.2012.709848 -
The Journal of Clinical Dentistry 2009The aim of this paper is to highlight recent advances in the development of analytical and in vitro methodologies to assess the potential efficacy of dentine... (Review)
Review
The aim of this paper is to highlight recent advances in the development of analytical and in vitro methodologies to assess the potential efficacy of dentine hypersensitivity treatments. The paper will cover the utility of X-ray computed nanotomography, secondary ion mass spectrometry, dynamic laser scanning confocal microscopy, and x-ray microtomography in the context of characterizing dentine, understanding the permeability and mass transport properties of dentine, and exploring the mechanism of action for purported hypersensitivity treatments. It will be shown that X-ray tomography, secondary ion mass spectrometry, and dynamic fluid flow confocal microscopy provide unsurpassed information pertaining to the ultra-structural and mass transport properties of dentine, properties that underpin the hydrodynamic theory and the therapeutic route for desensitizing treatments. The paper concludes that advances in analytical instrumentation and in vitro methodologies have improved our understanding of the ultra-structural and chemical properties of dentine, mass transport within dentine, and the quality and predictive value of in vitro models. Such understandings aid the development of efficacious dentifrice formulations to treat dentine hypersensitivity.
Topics: Chemistry Techniques, Analytical; Dentin; Dentin Desensitizing Agents; Dentin Permeability; Dentin Sensitivity; Humans
PubMed: 19902639
DOI: No ID Found -
Journal de Biologie Buccale Dec 1980The effect of 2 methods of demineralization on the preservation of proteoglycans and glycoproteins was studied in the intertubular and peritubular dentine of the horse....
The effect of 2 methods of demineralization on the preservation of proteoglycans and glycoproteins was studied in the intertubular and peritubular dentine of the horse. The specimens embedded in Epon were demineralized with a 2% acid formic solution (Bonucci and Gheradi, 1975). Other fragments were treated with an organic solution of EDTA alkylammonium salt (Scott and Kyffin, 1979). These methods preserved in a satisfactory way these labile organic components. In the intertubular dentine, glycoproteins and proteoglycans were also identified, either associated with collagen fibres as a glue and granules, or present in the interfibrillar spaces. In the peritubular dentine, amorphous material formed a fine network between the mineral structures.
Topics: Animals; Collagen; Crystallography; Decalcification Technique; Dentin; Edetic Acid; Formates; Glycoproteins; Horses; Proteoglycans
PubMed: 6783643
DOI: No ID Found -
Endodontics & Dental Traumatology Dec 1990The ability of osteoclasts to colonize in vitro different preparations of dentin extracted with guanidinium hydrochloride (GuHCl) was studied in order to establish...
The ability of osteoclasts to colonize in vitro different preparations of dentin extracted with guanidinium hydrochloride (GuHCl) was studied in order to establish morphological evidence for an extractable inhibitor of osteoclast spreading within dentin. Osteoclasts were isolated from neonatal rats and seeded onto pieces of fully mineralized dentin, demineralized dentin and predentin extracted with GuHCl. Osteoclasts were also seeded onto unextracted tissues. The results were evaluated with scanning electron microscopy. Osteoclasts colonized and resorbed fully mineralized dentin, whereas clastic cells were not observed on unextracted demineralized dentin and predentin. In contrast to this, osteoclasts attached and spread on demineralized dentin and predentin extracted with GuHCl. It was concluded that the non-collagenous organic component of dentin contains an extractable inhibitor of osteoclastic attachment and spreading. It is tempting to speculate that the inhibitor may be responsible for the naturally occurring resistance of dentin to resorption.
Topics: Animals; Cell Adhesion; Collagen; Dentin; Humans; Osteoclasts; Rats; Rats, Inbred Strains; Tooth Resorption
PubMed: 2094599
DOI: 10.1111/j.1600-9657.1990.tb00428.x -
New Zealand School Dental Service... Apr 1979
Topics: Dentin; Dentin Sensitivity; Humans; Odontoblasts; Osmotic Pressure
PubMed: 293630
DOI: No ID Found -
The Journal of Pedodontics 1986
Topics: Adolescent; Calcium Hydroxide; Child; Dentin; Hardness; Humans; Tooth Remineralization
PubMed: 3465950
DOI: No ID Found -
Journal of the Royal Society, Interface Dec 2016Teeth are key to understanding the feeding ecology of both extant and extinct vertebrates. Recent studies have highlighted the previously unrecognized complexity of...
Teeth are key to understanding the feeding ecology of both extant and extinct vertebrates. Recent studies have highlighted the previously unrecognized complexity of dinosaur dentitions and how specific tooth tissues and tooth shapes differ between taxa with different diets. However, it is unknown how the ultrastructure of these tooth tissues contributes to the differences in feeding style between taxa. In this study, we use third harmonic generation microscopy and scanning electron microscopy to examine the ultrastructure of the dentine in herbivorous and carnivorous dinosaurs to understand how the structure of this tissue contributes to the overall utility of the tooth. Morphometric analyses of dentinal tubule diameter, density and branching rates reveal a strong signal for dietary preferences, with herbivorous saurischian and ornithischian dinosaurs consistently having higher dentinal tubule density than their carnivorous relatives. We hypothesize that this relates to the hardness of the dentine, where herbivorous taxa have dentine that is more resistant to breakage and wear at the dentine-enamel junction than carnivorous taxa. This study advocates the detailed study of dentine and the use of advanced microscopy techniques to understand the evolution of dentition and feeding ecology in extinct vertebrates.
Topics: Animals; Carnivory; Dentin; Dinosaurs
PubMed: 27974573
DOI: 10.1098/rsif.2016.0626 -
Operative Dentistry 2003This study evaluated the dentinal composition and Knoop hardness measurements of the cavity floor following the removal of carious dentin by the Carisolv...
This study evaluated the dentinal composition and Knoop hardness measurements of the cavity floor following the removal of carious dentin by the Carisolv chemo-mechanical caries removal system, in vitro. The carious dentin of 25 extracted human teeth was removed by using Carisolv for one minute with instruments and excavation that was performed until the gel was clear. Caries removal with a sharp explorer was verified according to the color and hardness of the lesion, then, by means of DIAGNOdent. Atomic analysis of treated cavities was performed by energy dispersive x-ray spectroscopy (SEM-EDX) and the Knoop hardness number (KHN) of the cavity floor was determined. Surface characteristics were observed by the scanning electron microscope (SEM). Adjacent sound dentin was used as a control reference. No significant differences were found between the quantities of calcium content (Ca weight %), phosphorus content (P weight %) and the Ca/P weight ratio of Carisolv cavities with that of the adjacent, sound dentin (p<0.01). KHN of the Carisolv cavity floor was almost similar to that of the adjacent sound dentin. SEM analysis revealed an extremely rough or irregular surface, and there remained a minimal debris-like smear layer; most of the dentinal tubules were opened. The results indicated that Carisolv does not produce any adverse side effects on dentinal compositions of the treated cavities. The possibility of remaining residual softened dentin was also minimal in this study.
Topics: Dental Caries; Dental Cavity Preparation; Dentin; Electron Probe Microanalysis; Glutamic Acid; Hardness; Humans; Leucine; Lysine; Surface Properties
PubMed: 12877418
DOI: No ID Found -
Scientific Reports Feb 2019It is believed that penetration of dentinal fluid into natural enamel caries (NEC) is negligible because of the barrier created by underlying sclerotic dentine, but...
It is believed that penetration of dentinal fluid into natural enamel caries (NEC) is negligible because of the barrier created by underlying sclerotic dentine, but there are conflicting evidences on whether dentine subjacent to NEC is sclerotic or demineralized. This study aimed at investigating the relationship between NEC, subjacent dentine reactions, modification of dentinal fluid, and composition of cariogenic biofilm formed on the NEC surface. Proximal NEC (PNEC) lesions of human permanent posterior teeth were included in five experiments. Histologically, microradiographic analysis with contrast solution (MRC) in dentine revealed a decreased proportion of sclerotic dentine and an increased proportion of deep dentine demineralization compared to the classical stereomicroscopic histological analysis based on dentin color and translucency. Real-time MRC and 3D optical profilometry, and 3D microtomographic analysis evidenced a facilitated transport of modified dentinal fluid towards PNEC lesions. Cariogenic biofilm formed in vitro on the PNEC surface presented lower amounts of insoluble and soluble matrix polysaccharides when 2% chlorexidine was inserted in the pulp chamber. In conclusion, this study evidenced that dentine subjacent to PNEC is mostly demineralized, providing facilitated pathway for dentinal fluid to penetrate into PNEC and alter the composition of the biofilm formed on the PNEC surface.
Topics: Biofilms; Dental Caries; Dental Enamel; Dentin; Dentinal Fluid; Humans; Surface Properties; X-Ray Microtomography
PubMed: 30808878
DOI: 10.1038/s41598-019-38684-7