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Journal of Agricultural and Food... 1977
Topics: Dimethoate; Fruit; Insecticides; Methods; Pesticide Residues
PubMed: 881518
DOI: 10.1021/jf60212a026 -
Journal of Agricultural and Food... 1975
Topics: British Columbia; Dimethoate; Drug Stability; Evaluation Studies as Topic; Food Contamination; Fruit; Oxidation-Reduction; Pesticide Residues; Species Specificity; Time Factors
PubMed: 1141528
DOI: 10.1021/jf60200a058 -
Journal of Agricultural and Food... 1974
Comparative Study
Topics: Carbamates; Citrus; Dimethoate; Food Contamination; Organothiophosphorus Compounds; Pesticide Residues; Pesticide Synergists; Plants; Surface-Active Agents
PubMed: 4840571
DOI: 10.1021/jf60192a048 -
Journal of Agricultural and Food... 1981
Topics: Chromatography, Gas; Dimethoate; Insecticides; Pesticide Residues; Triticum
PubMed: 7276390
DOI: 10.1021/jf00106a046 -
Fa Yi Xue Za Zhi Jun 2018To study the decomposition kinetics of omethoate in blood.
OBJECTIVES
To study the decomposition kinetics of omethoate in blood.
METHODS
The acetonitrile precipitated protein was added into the blood, with the chromatographic column of a Waters BEH C column (2.1 mm×50 mm, 1.7 μm), the mobile phase of 5 mmol/L ammonium acetate aqueous solution-methanol, and the gradient elution with a flow rate of 0.3 mL/min and injection volume of 2 μL. With electrospray ionization (ESI) source and positive ion detection, qualitative and quantitative analyses were taken using multi-reaction monitoring mode. Omethoate standard was added into blank human blood to the mass concentrations of 0.78, 1.40, 2.30, 4.50, and 7.20 μg/mL, and each mass concentration was preserved at 3 temperatures of -20 ℃, 4 ℃, and 20 ℃, respectively. The content of omethoate was detected at different time points (0, 1, 3, 4, 7, 11, 15, 24, 32, 40, 48, 64, 80, 96, and 120 d).
RESULTS
Different concentrations of omethoate all showed a descended trend in human blood under different temperature conditions. The decomposition in storage environment of -20 ℃, 4 ℃, and 20 ℃ was fit to a one-compartment open model with a first-order kinetic process, which could be expressed as C=Ce, with the calculated theoretical values of omethoate concentration close to the measured values.
CONCLUSIONS
All concentrations of omethoate are decomposed in the blood, which vary a lot in different preservation conditions. It is suggested that blood samples should be frozen and detected timely in suspected omethoate poisoning cases.
Topics: Chromatography, High Pressure Liquid; Chromatography, Liquid; Dimethoate; Humans; Kinetics; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry
PubMed: 30896096
DOI: 10.12116/j.issn.1004-5619.2018.06.005 -
Xenobiotica; the Fate of Foreign... May 20231. Dimethoate is an organophosphate insecticide that is converted to omethoate, the active toxic moiety. Omethoate inhibits acetylcholinesterase (AChE) in the brain and...
1. Dimethoate is an organophosphate insecticide that is converted to omethoate, the active toxic moiety. Omethoate inhibits acetylcholinesterase (AChE) in the brain and red blood cells (RBCs). This paper describes the development of rat and human physiologically-based pharmacokinetic/pharmacodynamic (PBPK/PD) models for dimethoate.2. The model simulates the absorption and distribution of dimethoate and omethoate, the conversion of dimethoate to omethoate and to other metabolites, the metabolism and excretion of omethoate, and the inhibition of RBC and brain AChE. An extensive data collection program to estimate metabolism and inhibition parameters is described.3. The suite of models includes an adult rat, post-natal rat, and human model. The rat models were evaluated by comparing model predictions of dimethoate and omethoate to measured blood time course data, and with RBC and brain AChE inhibition estimates from an extensive database of AChE measurements.4. After the demonstration of adequately fitted rat models that were robust to sensitivity analysis, the human model was applied for estimation of points-of-departure (PODs) for risk assessment using the human-specific parameters in the human PBPK/PD model. Thus, the standard interspecies uncertainty factor can be reduced from 10X to 1X.
Topics: Adult; Rats; Humans; Animals; Insecticides; Dimethoate; Acetylcholinesterase
PubMed: 37706283
DOI: 10.1080/00498254.2023.2258507 -
International Journal of Environmental... May 2022To explore the association between polymorphisms in microRNAs (miRNAs) and the cholinesterase (ChE) activity in omethoate-exposed workers, we recruited 180...
To explore the association between polymorphisms in microRNAs (miRNAs) and the cholinesterase (ChE) activity in omethoate-exposed workers, we recruited 180 omethoate-exposed workers and 115 controls to measure their ChE activity using acetylcholine and dithio-bis-(nitrobenzoic acid) and genotype susceptible SNPs in their miRNA by time-of-flight mass spectrometry. ChE activity in the exposure group was lower than that in the control group ( < 0.001). The analysis of covariance result showed that ChE activity was lower in the (- -/- T) genotype in rs111456995 (1.97 ± 0.47) than in the TT genotype (2.23 ± 0.59) of the exposure group ( = 0.004). Multivariate linear regression was performed to find influencing factors on ChE activity, and variables kept in the model included omethoate exposure ( = -1.094, < 0.001), gender ( = -0.381, < 0.001), rs111456995 (- -/- T)( = -0.248, < 0.001), and drinking ( = 0.258, =0.019). The results suggest that individuals carrying a (- -/- T) genotype in rs111456995 were more susceptible to damage in their cholinesterase induced by omethoate exposure.
Topics: Case-Control Studies; Cholinesterases; Dimethoate; Genotype; Humans; MicroRNAs; Occupational Exposure; Polymorphism, Single Nucleotide
PubMed: 32962420
DOI: 10.1080/09603123.2020.1820453 -
Journal of Materials Chemistry. B Nov 2022Herein, the fluorescent enhancement of the split G-quadruplex (G4) towards DNA-templated AgNCs (DNA-AgNCs) was systematically studied. It was shown that the two G-rich...
Herein, the fluorescent enhancement of the split G-quadruplex (G4) towards DNA-templated AgNCs (DNA-AgNCs) was systematically studied. It was shown that the two G-rich probes with the ratio of the guanine (G) bases of 6 : 6 was the best split mode, and the spacer length of the zero T base at both two sides could present the optimal distance between the AgNCs and split G4. In light of these results, a split G4 coupled with DNA-AgNCs for omethoate (OME) detection was successfully fabricated. The developed fluorescent aptasensor could sense OME in the concentration range of 5-200 nM with a detection limit of 4.16 nM. Moreover, the aptasensor performed well in real-sample detection.
Topics: Fluorescence; Silver; Metal Nanoparticles; DNA
PubMed: 36314475
DOI: 10.1039/d2tb01755e -
Biomedical and Environmental Sciences :... May 2022
Topics: Cholinesterases; Dimethoate; Humans; Occupational Exposure; Polymorphism, Genetic; Telomere
PubMed: 35676815
DOI: 10.3967/bes2022.060 -
Journal - Association of Official... Nov 1972
Topics: Animals; Carbamates; Cattle; Chickens; Chromatography, Gas; Dimethoate; Eggs; Gels; Insecticides; Kidney; Liver; Methods; Milk; Muscles; Pesticide Residues; Plant Extracts; Plants, Edible; Silicon Dioxide; Tissue Extracts
PubMed: 4359930
DOI: No ID Found