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Fa Yi Xue Za Zhi Jun 2018To study the decomposition kinetics of omethoate in blood.
OBJECTIVES
To study the decomposition kinetics of omethoate in blood.
METHODS
The acetonitrile precipitated protein was added into the blood, with the chromatographic column of a Waters BEH C column (2.1 mm×50 mm, 1.7 μm), the mobile phase of 5 mmol/L ammonium acetate aqueous solution-methanol, and the gradient elution with a flow rate of 0.3 mL/min and injection volume of 2 μL. With electrospray ionization (ESI) source and positive ion detection, qualitative and quantitative analyses were taken using multi-reaction monitoring mode. Omethoate standard was added into blank human blood to the mass concentrations of 0.78, 1.40, 2.30, 4.50, and 7.20 μg/mL, and each mass concentration was preserved at 3 temperatures of -20 ℃, 4 ℃, and 20 ℃, respectively. The content of omethoate was detected at different time points (0, 1, 3, 4, 7, 11, 15, 24, 32, 40, 48, 64, 80, 96, and 120 d).
RESULTS
Different concentrations of omethoate all showed a descended trend in human blood under different temperature conditions. The decomposition in storage environment of -20 ℃, 4 ℃, and 20 ℃ was fit to a one-compartment open model with a first-order kinetic process, which could be expressed as C=Ce, with the calculated theoretical values of omethoate concentration close to the measured values.
CONCLUSIONS
All concentrations of omethoate are decomposed in the blood, which vary a lot in different preservation conditions. It is suggested that blood samples should be frozen and detected timely in suspected omethoate poisoning cases.
Topics: Chromatography, High Pressure Liquid; Chromatography, Liquid; Dimethoate; Humans; Kinetics; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry
PubMed: 30896096
DOI: 10.12116/j.issn.1004-5619.2018.06.005 -
Biomedical and Environmental Sciences :... May 2022
Topics: Cholinesterases; Dimethoate; Humans; Occupational Exposure; Polymorphism, Genetic; Telomere
PubMed: 35676815
DOI: 10.3967/bes2022.060 -
Biomedical and Environmental Sciences :... Oct 2021
Topics: Adult; China; Dimethoate; Farmers; Female; Humans; Male; Middle Aged; Occupational Exposure; Pesticides; Polymorphism, Single Nucleotide; Telomerase; Telomere
PubMed: 34782052
DOI: 10.3967/bes2021.115 -
Behavioral and Brain Functions : BBF Feb 2015To observe the effects of neuregulin1β (NRG1β) on the level of phosphorylated ERK1/2 (p-ERK1/2), and explore the therapeutic mechanism of NRG1β on the cognitive...
OBJECTIVE
To observe the effects of neuregulin1β (NRG1β) on the level of phosphorylated ERK1/2 (p-ERK1/2), and explore the therapeutic mechanism of NRG1β on the cognitive dysfunction in rats with chronic omethoate poisoning.
METHODS
Rats with strong learning and memory ability, 50 in total, were selected by Y-electric maze test. Among which, 15 rats were randomly selected into control group, and the rest 35 rats were used to establish experimental cognitive impairment models by being injected with omethoate subcutaneously. The 30 cases of successful cognitive impairment models were randomly divided into model group and treated group consisting of 15 rats, respectively. Then rats in treated group were injected with NRG1β into their lateral ventricles, while rats in control and model groups were given equal volume of PBS simultaneously. The cognitive capacity of rats was evaluated with Y-electric maze. The morphology and ultrastructure of hippocampus were observed by hematoxylin eosin (HE) staining and transmission electron microscopy (TEM) respectively. The expression of p-ERK1/2 was determined by immunohistochemical (IHC) staining and Western blotting.
RESULTS
Compared with rats in model group, the cognitive ability of rats with omethoate exposed (model and treated groups) reduced significantly, along with the obvious damage of hippocampal neurons and the expression of p-ERK1/2 decreased significantly (P < 0.05). And after treatment with NRG1β, the cognitive activity of treated rats was improved obviously, and the injury of hippocampal neurons was milder and the expression of p-ERK1/2 increased significantly more than those in model rats (P < 0.05).
CONCLUSION
In chronic omethoate poisoning rats, NRG1β can promote the phosphorylation level of ERK1/2 in hippocampal neurons, and play an important role in the improvement of cognitive function.
Topics: Animals; Cognition Disorders; Dimethoate; Extracellular Signal-Regulated MAP Kinases; Hippocampus; Injections, Intraventricular; Insecticides; Learning Disabilities; Male; Neuregulin-1; Neurons; Neuroprotective Agents; Neurotoxicity Syndromes; Phosphorylation; Rats; Rats, Wistar; Up-Regulation
PubMed: 25886297
DOI: 10.1186/s12993-014-0050-8 -
Shokuhin Eiseigaku Zasshi. Journal of... 2010We studied the simultaneous determination of acephate, methamidophos, and omethoate in animal and fishery products, their processed foods, and honey by means of liquid...
We studied the simultaneous determination of acephate, methamidophos, and omethoate in animal and fishery products, their processed foods, and honey by means of liquid chromatography coupled with mass spectrometry (LC-MS). The sample was extracted with ethyl acetate in the presence of anhydrous Na(2)SO(4) (and diatomaceous earth for honey). An aliquot of the crude sample extract was loaded into the GPC system, and the pesticide fraction was selectively collected. The extract was cleaned up on a PSA mini-column, and determined by a column-switching ESI-SIM mode LC-MS. Mean recoveries (2 replicates x 5 days) of compounds from eleven kinds of samples, except honey, fortified at the analyte concentration of 0.05 microg/g were from 71.4% to 98.4%. The repeatability relative standard deviation values were < or =12.5%, and the intermediate reproducibility relative standard deviation values were < or =14.1%. In honey, the recoveries were improved to 97.6-98.6% by using highly purified surrogates.
Topics: Animals; Chromatography, Liquid; Dimethoate; Eggs; Fish Products; Food Handling; Honey; Insecticides; Mass Spectrometry; Meat; Organothiophosphorus Compounds; Phosphoramides
PubMed: 20595793
DOI: 10.3358/shokueishi.51.122 -
Biomedical and Environmental Sciences :... Aug 2018
Topics: Animals; Blood Glucose; Cholesterol; Dimethoate; Insulin; Insulin Resistance; Liver; Male; Mice, Inbred ICR; Pesticides; Signal Transduction; Triglycerides
PubMed: 30231969
DOI: 10.3967/bes2018.086 -
Sensors (Basel, Switzerland) 2012This work presents the development of bioassays and biosensors for the detection of insecticides widely used in the treatment of olive trees. The systems are based on...
This work presents the development of bioassays and biosensors for the detection of insecticides widely used in the treatment of olive trees. The systems are based on the covalent immobilisation of acetylcholinesterase on magnetic microbeads using either colorimetry or amperometry as detection technique. The magnetic beads were immobilised on screen-printed electrodes or microtitration plates and tested using standard solutions and real samples. The developed devices showed good analytical performances with limits of detection much lower than the maximum residue limit tolerated by international regulations, as well as a good reproducibility and stability.
Topics: Acetylcholinesterase; Animals; Biosensing Techniques; Colorimetry; Dimethoate; Electrochemical Techniques; Enzymes, Immobilized; Inhibitory Concentration 50; Insecticides; Kinetics; Magnetics; Malathion; Microspheres; Olive Oil; Organophosphates; Organothiophosphorus Compounds; Pesticides; Plant Oils; Time Factors
PubMed: 22969377
DOI: 10.3390/s120607893 -
Tumour Biology : the Journal of the... Jul 2017Organophosphorous pesticides (OPs), with high efficiency, broad-spectrum and low residue, are widely used in China. Omethoate is a broad category of organophosphorous...
Organophosphorous pesticides (OPs), with high efficiency, broad-spectrum and low residue, are widely used in China. Omethoate is a broad category of organophosphorous pesticides and is more domestically utilized which has chronic toxic effect on human health caused by long-term, low-dose exposure to Ops, recently its potential genotoxicity has attracted wide attention which can cause chromosomal DNA damage. Thus, the aim of this study is screen susceptible biomarkers and explore the mechanism of canceration induced by omethoate. 180 long-term organophosphorus pesticide-exposed workers and 115 healthy controls were recruited. Quantitative polymerase chain reaction method was applied to determine the relative telomere length in peripheral lymphocyte DNA as well as p53 and p21 gene expression levels. Genetic polymorphisms were determined by the polymerase chain reaction-restriction fragment length polymorphism method. Multiple linear regression was conducted to explore the effects of exposure, expression levels, and polymorphisms in genes on the telomere length. The results showed the relative telomere lengths in the exposure group were significantly longer than that in the control group. The messenger RNA expression levels of p53 and p21 in exposure group were significantly lower than that in the control group; telomere lengths of the CA genotype individuals of p21 rs1801270 polymorphism locus were significantly longer than that of the CC genotype in the control group that were estimated using the Bonferroni method; and bivariate correlation analysis showed that the messenger RNA expression level of gene p53 was negatively correlated with telomere length, and the messenger RNA expression level of gene p21 was positively correlated with telomere length. Multivariate analysis found that p53 messenger RNA and p21 messenger RNA had an impact on telomere length. These results demonstrated that the messenger RNA expression levels of p53 and p21 may have a relationship with the changes in telomere length induced by omethoate and provided strong evidence for the mechanism of canceration induced by poison.
Topics: Adult; Carcinogenesis; Cell Cycle; China; Cyclin-Dependent Kinase Inhibitor p21; DNA Damage; Dimethoate; Female; Gene Expression Regulation, Neoplastic; Genotype; Humans; Lymphocytes; Male; Middle Aged; Neoplasms; Occupational Exposure; Polymorphism, Single Nucleotide; RNA, Messenger; Telomere Homeostasis; Tumor Suppressor Protein p53
PubMed: 28718371
DOI: 10.1177/1010428317719782 -
PloS One 2015Residue levels of dimethoate and its oxon metabolite (omethoate) during tea planting, manufacturing, and brewing were investigated using a modified QuEChERS sample...
Residue levels of dimethoate and its oxon metabolite (omethoate) during tea planting, manufacturing, and brewing were investigated using a modified QuEChERS sample preparation and gas chromatography. Dissipation of dimethoate and its metabolite in tea plantation followed the first-order kinetic with a half-life of 1.08-1.27 d. Tea manufacturing has positive effects on dimethoate dissipation. Processing factors of dimethoate are in the range of 2.11-2.41 and 1.41-1.70 during green tea and black tea manufacturing, respectively. Omethoate underwent generation as well as dissipation during tea manufacturing. Sum of dimethoate and omethoate led to a large portion of 80.5-84.9% transferring into tea infusion. Results of safety evaluation indicated that omethoate could bring higher human health risk than dimethoate due to its higher hazard quotient by drinking tea. These results would provide information for the establishment of maximum residue limit and instruction for the application of dimethoate formulation on tea crop.
Topics: Camellia sinensis; Dimethoate; Food Analysis; Food Contamination; Humans; Tea
PubMed: 26406463
DOI: 10.1371/journal.pone.0138309 -
Sensors (Basel, Switzerland) Jul 2019In this study, a hyperspectral imaging system of 866.4-1701.0 nm was selected and combined with multivariate methods to identify wheat kernels with different...
In this study, a hyperspectral imaging system of 866.4-1701.0 nm was selected and combined with multivariate methods to identify wheat kernels with different concentrations of omethoate on the surface. In order to obtain the optimal model combination, three preprocessing methods (standard normal variate (SNV), Savitzky-Golay first derivative (SG1), and multivariate scatter correction (MSC)), three feature extraction algorithms (successive projections algorithm (SPA), random frog (RF), and neighborhood component analysis (NCA)), and three classifier models (decision tree (DT), k-nearest neighbor (KNN), and support vector machine (SVM)) were applied to make a comparison. Firstly, based on the full wavelengths modeling analysis, it was found that the spectral data after MSC processing performed best in the three classifier models. Secondly, three feature extraction algorithms were used to extract the feature wavelength of MSC processed data and based on feature wavelengths modeling analysis. As a result, the MSC-NCA-SVM model performed best and was selected as the best model. Finally, in order to verify the reliability of the selected model, the hyperspectral image was substituted into the MSC-NCA-SVM model and the object-wise method was used to visualize the image classification. The overall classification accuracy of the four types of wheat kernels reached 98.75%, which indicates that the selected model is reliable.
Topics: Algorithms; Dimethoate; Edible Grain; Principal Component Analysis; Seeds; Spectroscopy, Near-Infrared; Support Vector Machine; Triticum
PubMed: 31319577
DOI: 10.3390/s19143147