Did you mean: edwardsiella hoshina
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Genome Announcements Feb 2017is a Gram-negative facultative anaerobe that has primarily been isolated from avians and reptiles. We report here the complete and annotated genome sequence of an...
is a Gram-negative facultative anaerobe that has primarily been isolated from avians and reptiles. We report here the complete and annotated genome sequence of an isolate from a monitor lizard ( sp.), which contains a chromosome of 3,811,650 bp and no plasmids.
PubMed: 28183769
DOI: 10.1128/genomeA.01605-16 -
Journal of Clinical Microbiology Sep 1991The pathogenic characteristics of 35 Edwardsiella strains from clinical and environmental sources were investigated. Overall, most Edwardsiella tarda strains were...
The pathogenic characteristics of 35 Edwardsiella strains from clinical and environmental sources were investigated. Overall, most Edwardsiella tarda strains were invasive in HEp-2 cell monolayers, produced a cell-associated hemolysin and siderophores, and bound Congo red; many strains also expressed mannose-resistant hemagglutination against guinea pig erythrocytes. Edwardsiella hoshinae strains bound Congo red and were variable in their invasive and hemolytic capabilities while Edwardsiella ictaluri strains did not produce either factor; neither E. hoshinae nor E. ictaluri expressed mannose-resistant hemagglutination nor elaborated siderophores under the tested conditions. Selected strains of each species tested for mouse lethality indicated strain variability in pathogenic potential, with E. tarda strains being the most virulent; 50% lethal doses in individual strains did not correlate with plasmid content, chemotactic motility, serum resistance, or expression of selected enzyme activities. The results suggest some potential important differences in pathogenic properties that may help explain their environmental distribution and ability to cause disease in humans.
Topics: Animals; Biomarkers; Enterobacteriaceae; Enterobacteriaceae Infections; Female; Hemolysin Proteins; Humans; Mice; Phenotype; Plasmids; Species Specificity; Virulence
PubMed: 1774326
DOI: 10.1128/jcm.29.9.1997-2001.1991 -
Antimicrobial Agents and Chemotherapy Aug 2001The natural antibiotic susceptibilities to 71 antibiotics of 102 Edwardsiella strains belonging to E. tarda (n = 42), E. ictaluri (n = 41), and E. hoshinae (n = 19) were... (Comparative Study)
Comparative Study
The natural antibiotic susceptibilities to 71 antibiotics of 102 Edwardsiella strains belonging to E. tarda (n = 42), E. ictaluri (n = 41), and E. hoshinae (n = 19) were investigated. MICs were determined using a microdilution procedure according to NCCLS criteria and German standards. All edwardsiellae were naturally sensitive to tetracyclines, aminoglycosides, most beta-lactams, quinolones, antifolates, chloramphenicol, nitrofurantoin, and fosfomycin. Edwardsiella species were naturally resistant to macrolides, lincosamides, streptogramins, glycopeptides, rifampin, fusidic acid, and oxacillin. Although slight species-dependent differences in natural susceptibilities to some antibiotics (e.g., macrolides and cefaclor) were seen, differences in natural susceptibility affecting clinical assessment criteria were only seen with benzylpenicillin. Whereas E. tarda was naturally resistant to benzylpenicillin, E. hoshinae was naturally sensitive. Natural sensitivity and resistance to this penicillin were found among the strains of E. ictaluri. The observed oxacillin sensitivity of E. ictaluri was attributed to the failure of the species to grow at higher salt concentrations found in oxacillin-containing microtiter plates. The present study describes a database concerning the natural susceptibility of Edwardsiella species to a wide range of antibiotics, which can be applied to validate forthcoming antibiotic susceptibility tests of these microorganisms.
Topics: Anti-Bacterial Agents; Databases, Factual; Drug Resistance, Microbial; Edwardsiella ictaluri; Edwardsiella tarda; Humans; Microbial Sensitivity Tests
PubMed: 11451681
DOI: 10.1128/AAC.45.8.2245-2255.2001 -
The Veterinary Record Jul 2004
Topics: Animals; Animals, Wild; Anti-Bacterial Agents; Edwardsiella; Kansas; Microbial Sensitivity Tests; Quail
PubMed: 15264489
DOI: 10.1136/vr.155.1.29 -
International Journal of Molecular... Mar 2023The genus presents five different pathogenic species: , , , and . These species cause infections mainly in fish, but they can also infect reptiles, birds or humans....
The genus presents five different pathogenic species: , , , and . These species cause infections mainly in fish, but they can also infect reptiles, birds or humans. Lipopolysaccharide (endotoxin) plays an important role in the pathogenesis of these bacteria. For the first time, the chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharides of , , and were studied. The complete gene assignments for all core biosynthesis gene functions were acquired. The structure of core oligosaccharides was investigated by ¹H and C nuclear magnetic resonance (NMR) spectroscopy. The structures of and core oligosaccharides show the presence of →3,4)-L--α-D--Hep, two terminal β-D-Glc, →2,3,7)-L--α-D--Hep, →7)-L--α-D--Hep, terminal α-D-GlcN, two →4)-α-D-GalA, → 3)-α-D-GlcNAc, terminal β-D-Gal and →5-substituted Kdo. core oligosaccharide shows only one terminal β-D-Glc, and instead of terminal β-D-Gal a terminal α-D-GlcNAc. core oligosaccharide shows only one terminal β-D-Glc, one →4)-α-D-GalA and do not have terminal α-D-GlcN (see complementary figure).
Topics: Animals; Humans; Carbohydrate Sequence; Lipopolysaccharides; Oligosaccharides; Magnetic Resonance Spectroscopy; Methylation
PubMed: 36902212
DOI: 10.3390/ijms24054768 -
Journal of Medical Microbiology Oct 1993Antimicrobial susceptibility profiles of clinical and environmental isolates of Edwardsiella demonstrate that the three species are susceptible to beta-lactam... (Comparative Study)
Comparative Study
Comparison of antimicrobial susceptibility, beta-lactamase production, plasmid analysis and serum bactericidal activity in Edwardsiella tarda, E. ictaluri and E. hoshinae.
Antimicrobial susceptibility profiles of clinical and environmental isolates of Edwardsiella demonstrate that the three species are susceptible to beta-lactam antibiotics. All strains were susceptible to two quinolones tested and to gentamicin and doxycycline. E. tarda and E. hoshinae were resistant to clindamycin, whereas E. ictaluri was moderately susceptible. beta-Lactamase was produced by all strains of E. tarda, but not by E. hoshinae or E. ictaluri. A 54-kb plasmid was detected in six of 13 E. hoshinae strains. Five of the 10 E. tarda isolates studied gave an identical plasmid pattern of four plasmids ranging in size from 76-kb to 5.0-kb. One strain exhibited a 54-kb plasmid; four strains did not contain plasmid DNA. All E. ictaluri isolates contained a 5.7-kb and a 4.9-kb plasmid. E. tarda and E. ictaluri strains were resistant to human serum 20%; 12 of 13 strains of E. hoshinae were also serum resistant. Serum resistance may play an important part in the pathogenicity of these species.
Topics: Anti-Bacterial Agents; Blood Bactericidal Activity; Enterobacteriaceae; Humans; Microbial Sensitivity Tests; Plasmids; Species Specificity; Virulence; beta-Lactamases
PubMed: 8411088
DOI: 10.1099/00222615-39-4-273 -
Systematic and Applied Microbiology Jan 2018Until 2012, the genus Edwardsiella was composed by three species Edwardsiella tarda, Edwardsiella hoshinae and Edwardsiella ictaluri. In 2013, Edwardsiella piscicida,...
Until 2012, the genus Edwardsiella was composed by three species Edwardsiella tarda, Edwardsiella hoshinae and Edwardsiella ictaluri. In 2013, Edwardsiella piscicida, compiling fish pathogenic strains previously identified as E. tarda was described, and more recently a new species isolated from diseased eel was reported, namely Edwardsiella anguillarum. The incorporation of these species into the genus makes necessary a revision of the taxonomic position of the isolates previously identified as E. tarda. Using AFLP technique, MLSA studies and in silico DNA-DNA hybridization, 46 of 49 E. tarda isolates were re-assigned as E. piscicida and 2 as E. anguillarum, whereas it was confirmed previous classification of the Edwardsiella types and reference strains used. The study of the taxonomic resolution of the genes 16S rRNA, adk, atpD, dnaJ, glnA, hsp60, tuf as well as the possible combinations among housekeeping genes, showed that the gene dnaJ was the more resolutive. In conclusion, the use of molecular techniques is necessary to accurately identify Edwardsiella isolates, especially when differentiating new species from E. tarda.
Topics: Amplified Fragment Length Polymorphism Analysis; Animals; Bacterial Proteins; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; Edwardsiella; Fishes; Multilocus Sequence Typing; Nucleic Acid Hybridization; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 29150173
DOI: 10.1016/j.syapm.2017.09.004 -
Infection and Immunity Jan 1991The ability of 22 Edwardsiella strains to penetrate and replicate in cultured epithelial cells was initially evaluated by light microscopy methods and by the recovery of...
The ability of 22 Edwardsiella strains to penetrate and replicate in cultured epithelial cells was initially evaluated by light microscopy methods and by the recovery of gentamicin-resistant (Gmr) bacteria from the Triton X-100 cell lysates of HEp-2-infected monolayers. Giemsa-stained HEp-2 cells revealed the presence of numerous internalized bacteria 3 h postinfection, often appearing as parallel rows of replicated bacteria within the cytosol and sometimes obliterating the cytoplasm because of the large numbers of bacilli present. Invasive bacteria were also sometimes found within cytoplasmic vacuoles in infected cells; thin-section electron micrographs of HEp-2-infected cells supported these conclusions. Results of light microscopy studies and cell lysate assays indicated that most Edwardsiella tarda (92%) and some Edwardsiella hoshinae strains were invasion positive on one or more occasions, while Edwardsiella ictaluri isolates were uniformly negative. HEp-2 invasion by E. tarda was a microfilament-dependent (cytochalasin B- and D-sensitive) process, with maximum numbers of Gmr CFU recorded between 3 and 6 h postinfection. The small percentage (0.01 to 1.0%) of the challenge inoculum recoverable as Gmr progeny 3 to 6 h postinfection was attributed to a strong cell-associated (not filterable) hemolysin that was produced by a majority (85%) of the E. tarda strains but not by E. ictaluri and only minimally by E. hoshinae. This cytolysin/hemolysin was responsible for the toxic effects observed in HEp-2 cells during the infection-replication process of edwardsiellae and appears to play a role in the release of internalized and replicated bacteria from infected cells. The results suggest an invasion strategy with some similarities to and differences from those of other recognized enteroinvasive pathogens.
Topics: Cells, Cultured; Enterobacteriaceae; Epithelium; Hemolysin Proteins; Shigella; Virulence
PubMed: 1987028
DOI: 10.1128/iai.59.1.154-161.1991 -
Journal of Fish Diseases Jun 2019The genus Edwardsiella is one of the major causes of fish diseases globally. Herein, we examined 37 isolates from ten different fish species from India, South Korea and...
The genus Edwardsiella is one of the major causes of fish diseases globally. Herein, we examined 37 isolates from ten different fish species from India, South Korea and Taiwan to gain insight into their phenotypic and genotypic properties, of which 30 were characterized as E. tarda with phenotypic homology estimated at 85.71% based on API-20E biochemical tests. Genotyping using 16S rRNA put all isolates together with E. anguillarum, E. hoshinae, E. tarda, E. piscicida and E. ictaluri reference strains in a monophyletic group. In contrast, the gyrB phylogenetic tree clearly separated E. ictaluri, E. tarda and E. hoshinae reference strains from our isolates and put our isolates into two groups with group I being homologous with the E. anguillarum reference strain while group II was homologous with the E. piscicida reference strain. Hence, our findings point to E. piscicida and E. anguillarum as species infecting different fish species in Asia. Homology of the ompW protein suggested that strains with broad protective coverage could be identified as vaccine candidates. This study underscores the importance of combining genotyping with phenotyping for valid species classification. In addition, it accentuates the importance of phylogenetic comparison of bacterial antigens for identification of potential vaccine candidates.
Topics: Animals; Aquaculture; Asia; Bacterial Vaccines; DNA, Bacterial; Disease Outbreaks; Edwardsiella; Edwardsiella tarda; Enterobacteriaceae Infections; Fish Diseases; Fishes; Genotype; Geography; India; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Seafood; Sequence Analysis, DNA
PubMed: 30851008
DOI: 10.1111/jfd.12984 -
Journal of Fish Diseases Sep 2018This report describes a case of systemic bacterial infection caused by Edwardsiella tarda in a Western African lungfish (Protopterus annectens) exposed to poor...
This report describes a case of systemic bacterial infection caused by Edwardsiella tarda in a Western African lungfish (Protopterus annectens) exposed to poor environmental and husbandry conditions. The fish presented with a large, external ulcerative lesion and died 2 weeks after developing anorexia. Histological evaluation revealed multifocal areas of necrosis and heterophilic and histiocytic inflammation throughout multiple tissues. Gram stain identified small numbers of intra- and extracellular monomorphic Gram-negative 1 to 2 μm rod-shaped bacilli. Cytology of lung granuloma, kidney and testes imprints identified heterophilic inflammation with phagocytosis of small monomorphic bacilli and some heterophils exhibiting cytoplasmic projections indicative of heterophil extracellular traps (HETs). Initial phenotypic analysis of isolates from coelomic fluid cultures identified E. tarda. Subsequent molecular analysis of spleen, liver and intestine DNA using an E. tarda-specific endpoint PCR assay targeting the bacterial fimbrial subunit yielded a 115 bp band. Sequencing and BLASTN search revealed the sequence was identical (76/76) to E. tarda strain FL95-01 (GenBank acc. CP011359) and displayed 93% sequence identity (66/71) to Edwardsiella hoshinae strain ATCC 35051 (GenBank acc. CP011359). This is the first report of systemic edwardsiellosis in a lungfish with concurrent cytologically identified structures suggestive of HETs.
Topics: Animals; Anorexia; Cytological Techniques; DNA, Bacterial; Edwardsiella tarda; Enterobacteriaceae Infections; Extracellular Traps; Fish Diseases; Fishes; Granulocytes; Kidney; Lung; Male; Phylogeny; Polymerase Chain Reaction; Sepsis; Testis
PubMed: 29882594
DOI: 10.1111/jfd.12831